(Circular) (Six-base) MAPSORT of: pFN21AB8396.seq Check: 9861 from: 1 to: 6097 pFN21AB8396 6097 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3907 278 Size: 53 83 186 936 2371 2468 Fragments arranged by size: 2468 2371 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3440 1937 Size: 1503 4594 Acc65I G'GTAC_C Cuts at: 3418 3418 Size: 6097 AclI AA'CG_TT Cuts at: 1563 4224 4597 1563 Size: 2661 373 3063 Fragments arranged by size: 3063 2661 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4160 5544 5812 1632 Size: 2528 1384 268 1917 Fragments arranged by size: 2528 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5900 AflIII A'CryG_T Cuts at: 1203 2289 4996 5973 1203 Size: 1086 2707 977 1327 Fragments arranged by size: 2707 1327 1086 977 AgeI A'CCGG_T Cuts at: 4928 4928 Size: 6097 AhdI GACnn_n'nnGTC Cuts at: 3196 4826 3196 Size: 1630 4467 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6097 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3015 1184 Size: 1831 4266 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2276 2308 2276 Size: 32 6065 AlwNI CAG_nnn'CTG Cuts at: 3840 5412 3840 Size: 1572 4525 ApaI G_GGCC'C Cuts at: 2654 2698 2654 Size: 44 6053 ApaLI G'TGCA_C Cuts at: 4154 5310 4154 Size: 1156 4941 ApoI r'AATT_y Cuts at: 1493 3406 3632 3784 5893 1493 Size: 1913 226 152 2109 1697 Fragments arranged by size: 2109 1913 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4651 160 Size: 872 3619 1606 Fragments arranged by size: 3619 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6097 AvaI C'yCGr_G Cuts at: 1870 1943 2060 2137 2227 2452 2665 2698 2933 Size: 73 117 77 90 225 213 33 235 Cuts at: 2933 3422 1870 Size: 489 4545 Fragments arranged by size: 4545 489 235 225 213 117 90 77 73 33 BamHI G'GATC_C Cuts at: 2566 3427 3794 2566 Size: 861 367 4869 Fragments arranged by size: 4869 861 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2462 3418 4873 618 Size: 325 217 627 675 956 1455 1842 Fragments arranged by size: 1842 1455 956 675 627 325 217 BanII G_rGCy'C Cuts at: 1391 2226 2541 2654 2698 2944 3119 3416 1391 Size: 835 315 113 44 246 175 297 4072 Fragments arranged by size: 4072 835 315 297 246 175 113 44 BbeI G_GCGC'C Cuts at: 2466 2466 Size: 6097 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2572 928 Size: 971 673 4453 Fragments arranged by size: 4453 971 673 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4288 4322 1903 Size: 34 50 34 2267 34 3678 Fragments arranged by size: 3678 2267 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3989 5199 3989 Size: 1210 4887 BclI T'GATC_A Cuts at: 1548 1548 Size: 6097 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2549 2645 2669 Size: 107 122 71 631 605 877 96 24 Cuts at: 2669 2724 4708 136 Size: 55 1984 1525 Fragments arranged by size: 1984 1525 877 631 605 122 107 96 71 55 24 BglII A'GATC_T Cuts at: 6092 6092 Size: 6097 BlpI GC'TnA_GC Cuts at: 3514 3514 Size: 6097 Bme1580I G_kGCm'C Cuts at: 1790 2490 2622 2641 2654 2698 4158 5314 1790 Size: 700 132 19 13 44 1460 1156 2573 Fragments arranged by size: 2573 1460 1156 700 132 44 19 13 BmgBI CAC'GTC Cuts at: 1318 2022 2839 3074 1318 Size: 704 817 235 4341 Fragments arranged by size: 4341 817 704 235 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2318 2440 3828 Size: 416 53 83 399 24 901 122 1388 Cuts at: 3828 4786 442 Size: 958 1753 Fragments arranged by size: 1753 1388 958 901 416 399 122 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6097 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2086 2233 2086 Size: 147 5950 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2324 2402 3191 3287 4757 1239 Size: 1085 78 789 96 1470 2579 Fragments arranged by size: 2579 1470 1085 789 96 78 Bpu10I CC'TnA_GC Cuts at: 2783 3215 2783 Size: 432 5665 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2273 3065 3133 4222 5087 5385 2273 Size: 792 68 1089 865 298 2985 Fragments arranged by size: 2985 1089 865 792 298 68 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4760 882 Size: 617 3261 2219 Fragments arranged by size: 3261 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3019 3226 3888 493 Size: 711 1815 207 662 2702 Fragments arranged by size: 2702 1815 711 662 207 BsaBI GATnn'nnATC Cuts at: 6091 6091 Size: 6097 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2463 3065 3904 4286 Size: 53 83 186 936 930 602 839 382 Cuts at: 4286 4921 275 Size: 635 1451 Fragments arranged by size: 1451 936 930 839 635 602 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2768 4530 4928 5202 5349 5760 2768 Size: 1762 398 274 147 411 3105 Fragments arranged by size: 3105 1762 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2276 2306 1178 Size: 30 165 30 873 30 4969 Fragments arranged by size: 4969 873 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2297 2857 3326 1186 Size: 1111 560 469 3957 Fragments arranged by size: 3957 1111 560 469 BseYI C'CCAG_C Cuts at: 2192 2232 2686 5300 5796 2192 Size: 40 454 2614 496 2493 Fragments arranged by size: 2614 2493 496 454 40 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5524 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2280 2416 2512 2553 2661 2728 Size: 275 186 279 136 96 41 108 67 Cuts at: 2728 3547 4308 5336 1540 Size: 819 761 1028 2301 Fragments arranged by size: 2301 1028 819 761 279 275 186 136 108 96 67 41 BsiHKAI G_wGCw'C Cuts at: 2214 2541 3318 4158 4243 5314 2214 Size: 327 777 840 85 1071 2997 Fragments arranged by size: 2997 1071 840 777 327 85 BsmI GAATG_Cn' Cuts at: 3608 3701 3608 Size: 93 6004 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2214 2226 2490 2541 2622 2641 2654 Size: 399 424 12 264 51 81 19 13 Cuts at: 2654 2698 2944 3119 3318 3416 4158 4243 5314 Size: 44 246 175 199 98 742 85 1071 Cuts at: 5314 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 424 399 264 246 199 175 98 85 81 51 44 19 13 12 BspEI T'CCGG_A Cuts at: 2768 5760 2768 Size: 2992 3105 BspHI T'CATG_A Cuts at: 3986 5716 3986 Size: 1730 4367 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3452 844 Size: 2608 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2358 3079 3984 1394 Size: 429 535 721 905 3507 Fragments arranged by size: 3507 905 721 535 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4592 4766 65 Size: 1183 3344 174 1396 Fragments arranged by size: 3344 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2432 2459 2507 4741 4928 1700 Size: 110 622 27 48 2234 187 2869 Fragments arranged by size: 2869 2234 622 187 110 48 27 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6097 BssHII G'CGCG_C Cuts at: 1925 2386 1925 Size: 461 5636 BssSI C'ACGA_G Cuts at: 4157 5169 4157 Size: 1012 5085 BstAPI GCAn_nnn'nTGC Cuts at: 794 2043 3364 794 Size: 1249 1321 3527 Fragments arranged by size: 3527 1321 1249 BstBI TT'CG_AA Cuts at: 4901 4901 Size: 6097 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6097 BstYI r'GATC_y Cuts at: 1972 2566 3427 3794 4179 4196 5648 5734 5746 Size: 594 861 367 385 17 1452 86 12 Cuts at: 5746 6092 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 861 594 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2197 2416 2574 513 Size: 552 1132 219 158 4036 Fragments arranged by size: 4036 1132 552 219 158 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5358 Fragments arranged by size: 5358 683 56 BtsI GCAGTG_nn' Cuts at: 3613 4407 4427 3613 Size: 794 20 5283 Fragments arranged by size: 5283 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6097 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6062 DraI TTT'AAA Cuts at: 3401 3401 Size: 6097 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5104 809 Size: 507 3788 1802 Fragments arranged by size: 3788 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2277 2413 2509 Size: 54 1425 50 275 18 447 136 96 Cuts at: 2509 2550 2631 2725 3544 4433 6062 8 Size: 41 81 94 819 889 1629 43 Fragments arranged by size: 1629 1425 889 819 447 275 136 96 94 81 54 50 43 41 18 EagI C'GGCC_G Cuts at: 1537 1812 2277 2413 2509 2550 2725 3544 1537 Size: 275 465 136 96 41 175 819 4090 Fragments arranged by size: 4090 819 465 275 175 136 96 41 EarI CTCTTCn'nnn_ Cuts at: 1353 2805 2817 4027 1353 Size: 1452 12 1210 3423 Fragments arranged by size: 3423 1452 1210 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2356 4684 5058 5204 387 Size: 1064 138 767 2328 374 146 1280 Fragments arranged by size: 2328 1280 1064 767 374 146 138 EcoICRI GAG'CTC Cuts at: 2539 2539 Size: 6097 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2324 2402 3191 3287 4160 4757 5544 Size: 393 692 78 789 96 873 597 787 Cuts at: 5544 5812 1239 Size: 268 1524 Fragments arranged by size: 1524 873 789 787 692 597 393 268 96 78 EcoO109I rG'GnC_Cy Cuts at: 2651 2694 2695 2755 2930 2651 Size: 43 1 60 175 5818 Fragments arranged by size: 5818 175 60 43 1 EcoRI G'AATT_C Cuts at: 3406 3784 3406 Size: 378 5719 FspI TGC'GCA Cuts at: 4603 4603 Size: 6097 HaeII r_GCGC'y Cuts at: 1678 2466 2615 3178 3184 5244 1678 Size: 788 149 563 6 2060 2531 Fragments arranged by size: 2531 2060 788 563 149 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3182 3214 4738 4770 4812 Size: 32 135 32 1610 32 1524 32 42 Cuts at: 4812 4844 1373 Size: 32 2626 Fragments arranged by size: 2626 1610 1524 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3441 3687 669 Size: 988 281 1503 246 3079 Fragments arranged by size: 3079 1503 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6097 HpaI GTT'AAC Cuts at: 3687 3687 Size: 6097 KasI G'GCGC_C Cuts at: 2462 2462 Size: 6097 KpnI G_GTAC'C Cuts at: 3422 3422 Size: 6097 MfeI C'AATT_G Cuts at: 3696 3696 Size: 6097 MluI A'CGCG_T Cuts at: 2289 5973 2289 Size: 3684 2413 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2895 5211 5395 1305 Size: 1590 2316 184 2007 Fragments arranged by size: 2316 2007 1590 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4275 Fragments arranged by size: 4275 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2243 2903 2997 4055 4414 4573 518 Size: 648 1077 660 94 1058 359 159 2042 Fragments arranged by size: 2042 1077 1058 660 648 359 159 94 MspA1I CmG'CkG Cuts at: 1606 2185 2418 2739 3513 4190 5338 5583 1606 Size: 579 233 321 774 677 1148 245 2120 Fragments arranged by size: 2120 1148 774 677 579 321 245 233 NaeI GCC'GGC Cuts at: 2461 2509 2461 Size: 48 6049 NarI GG'CG_CC Cuts at: 2463 2463 Size: 6097 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5545 NdeI CA'TA_TG Cuts at: 387 387 Size: 6097 NgoMIV G'CCGG_C Cuts at: 2459 2507 2459 Size: 48 6049 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6097 NotI GC'GGCC_GC Cuts at: 2725 3544 2725 Size: 819 5278 NspI r_CATG'y Cuts at: 1132 1229 2353 3455 5000 1132 Size: 97 1124 1102 1545 2229 Fragments arranged by size: 2229 1545 1124 1102 97 PasI CC'CwG_GG Cuts at: 1348 2926 1348 Size: 1578 4519 PciI A'CATG_T Cuts at: 4996 4996 Size: 6097 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6097 PfoI T'CCnGG_A Cuts at: 2340 3168 2340 Size: 828 5269 PmeI GTTT'AAAC Cuts at: 3401 3401 Size: 6097 PmlI CAC'GTG Cuts at: 3226 3226 Size: 6097 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2276 2308 4166 4198 5679 5711 2276 Size: 32 1858 32 1481 32 2662 Fragments arranged by size: 2662 1858 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2930 2930 Size: 6097 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6097 PsiI TTA'TAA Cuts at: 3667 3667 Size: 6097 PspOMI G'GGCC_C Cuts at: 2650 2694 2650 Size: 44 6053 PspXI vC'TCGA_Gb Cuts at: 2060 2060 Size: 6097 PstI C_TGCA'G Cuts at: 830 2409 3449 830 Size: 1579 1040 3478 Fragments arranged by size: 3478 1579 1040 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6097 SacI G_AGCT'C Cuts at: 2541 2541 Size: 6097 SacII CC_GC'GG Cuts at: 2419 2419 Size: 6097 SalI G'TCGA_C Cuts at: 1936 3439 1936 Size: 1503 4594 SanDI GG'GwC_CC Cuts at: 2930 2930 Size: 6097 SbfI CC_TGCA'GG Cuts at: 3449 3449 Size: 6097 ScaI AGT'ACT Cuts at: 4345 6057 4345 Size: 1712 4385 SfcI C'TryA_G Cuts at: 826 1045 2075 2405 3445 4580 5261 5452 826 Size: 219 1030 330 1040 1135 681 191 1471 Fragments arranged by size: 1471 1135 1040 1030 681 330 219 191 SfoI GGC'GCC Cuts at: 2464 2464 Size: 6097 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6097 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6097 SmaI CCC'GGG Cuts at: 1872 2139 2454 2700 2935 3424 1872 Size: 267 315 246 235 489 4545 Fragments arranged by size: 4545 489 315 267 246 235 SmlI C'TyrA_G Cuts at: 820 1017 1943 2060 2252 3080 3112 4201 5102 Size: 197 926 117 192 828 32 1089 901 Cuts at: 5102 5364 820 Size: 262 1553 Fragments arranged by size: 1553 1089 926 901 828 262 197 192 117 32 SnaBI TAC'GTA Cuts at: 493 3888 493 Size: 3395 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6097 SphI G_CATG'C Cuts at: 1132 3455 1132 Size: 2323 3774 SspI AAT'ATT Cuts at: 5 52 4021 5 Size: 47 3969 2081 Fragments arranged by size: 3969 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6097 StyI C'CwwG_G Cuts at: 513 1065 2877 3258 3536 513 Size: 552 1812 381 278 3074 Fragments arranged by size: 3074 1812 552 381 278 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2300 3186 4124 4141 4294 4479 4924 Size: 1255 720 886 938 17 153 185 445 Cuts at: 4924 325 Size: 1498 Fragments arranged by size: 1498 1255 938 886 720 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4343 6055 96 Size: 274 80 33 51 1443 2366 1712 138 Fragments arranged by size: 2366 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2336 2368 2893 2925 6057 6089 2336 Size: 32 525 32 3132 32 2344 Fragments arranged by size: 3132 2344 525 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2840 1316 Size: 1524 4573 XbaI T'CTAG_A Cuts at: 3433 3433 Size: 6097 XhoI C'TCGA_G Cuts at: 1943 2060 1943 Size: 117 5980 XmaI C'CCGG_G Cuts at: 1870 2137 2452 2698 2933 3422 1870 Size: 267 315 246 235 489 4545 Fragments arranged by size: 4545 489 315 267 246 235 XmnI GAAnn'nnTTC Cuts at: 4226 4226 Size: 6097 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3905 276 Size: 53 83 186 936 2371 2468 Fragments arranged by size: 2468 2371 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PsrI PvuII RsrII SapI SexAI SfiI SrfI SwaI XcmI