(Circular) (Six-base) MAPSORT of: pFN21AB7638.seq Check: 2274 from: 1 to: 5848 pFN21AB7638 5848 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3658 278 Size: 53 83 186 936 2122 2468 Fragments arranged by size: 2468 2122 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2677 3191 1937 Size: 740 514 4594 Fragments arranged by size: 4594 740 514 Acc65I G'GTAC_C Cuts at: 3169 3169 Size: 5848 AclI AA'CG_TT Cuts at: 1563 3975 4348 1563 Size: 2412 373 3063 Fragments arranged by size: 3063 2412 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2478 2725 3911 5295 5563 1632 Size: 846 247 1186 1384 268 1917 Fragments arranged by size: 1917 1384 1186 846 268 247 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5651 AflIII A'CryG_T Cuts at: 1203 4747 5724 1203 Size: 3544 977 1327 Fragments arranged by size: 3544 1327 977 AgeI A'CCGG_T Cuts at: 4679 4679 Size: 5848 AhdI GACnn_n'nnGTC Cuts at: 2141 4577 2141 Size: 2436 3412 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5848 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2334 1184 Size: 1150 4698 AlwNI CAG_nnn'CTG Cuts at: 2886 3121 3591 5163 2886 Size: 235 470 1572 3571 Fragments arranged by size: 3571 1572 470 235 ApaI G_GGCC'C Cuts at: 2152 2333 2847 2969 2152 Size: 181 514 122 5031 Fragments arranged by size: 5031 514 181 122 ApaLI G'TGCA_C Cuts at: 3905 5061 3905 Size: 1156 4692 ApoI r'AATT_y Cuts at: 1493 2424 3157 3383 3535 5644 1493 Size: 931 733 226 152 2109 1697 Fragments arranged by size: 2109 1697 931 733 226 152 AseI AT'TA_AT Cuts at: 160 1032 4402 160 Size: 872 3370 1606 Fragments arranged by size: 3370 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5848 AvaI C'yCGr_G Cuts at: 1870 1943 2264 2622 2754 3173 1870 Size: 73 321 358 132 419 4545 Fragments arranged by size: 4545 419 358 321 132 73 BamHI G'GATC_C Cuts at: 2669 3178 3545 2669 Size: 509 367 4972 Fragments arranged by size: 4972 509 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3027 3169 4624 618 Size: 325 217 627 1240 142 1455 1842 Fragments arranged by size: 1842 1455 1240 627 325 217 142 BanII G_rGCy'C Cuts at: 1391 2152 2333 2699 2755 2847 2969 2992 3167 Size: 761 181 366 56 92 122 23 175 Cuts at: 3167 1391 Size: 4072 Fragments arranged by size: 4072 761 366 181 175 122 92 56 23 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4877 BbvCI CC'TCA_GC Cuts at: 2691 2691 Size: 5848 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2171 2205 2222 2256 4039 4073 1903 Size: 34 234 34 17 34 1783 34 3678 Fragments arranged by size: 3678 1783 234 34 34 34 34 17 BciVI GTATCCnnnnn_n' Cuts at: 3740 4950 3740 Size: 1210 4638 BclI T'GATC_A Cuts at: 1548 1548 Size: 5848 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2795 2957 4459 Size: 107 122 71 631 605 1123 162 1502 Cuts at: 4459 136 Size: 1525 Fragments arranged by size: 1525 1502 1123 631 605 162 122 107 71 BglII A'GATC_T Cuts at: 5843 5843 Size: 5848 BlpI GC'TnA_GC Cuts at: 2366 3265 2366 Size: 899 4949 Bme1580I G_kGCm'C Cuts at: 1790 2152 2227 2333 2847 2907 2969 3909 5065 Size: 362 75 106 514 60 62 940 1156 Cuts at: 5065 1790 Size: 2573 Fragments arranged by size: 2573 1156 940 514 362 106 75 62 60 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5848 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3579 4537 442 Size: 416 53 83 399 24 2162 958 1753 Fragments arranged by size: 2162 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5848 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2614 2614 Size: 5848 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2147 2495 2543 2683 4508 1239 Size: 908 348 48 140 1825 2579 Fragments arranged by size: 2579 1825 908 348 140 48 Bpu10I CC'TnA_GC Cuts at: 2691 2691 Size: 5848 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3025 3128 3973 4838 5136 3025 Size: 103 845 865 298 3737 Fragments arranged by size: 3737 865 845 298 103 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2405 3062 4511 882 Size: 617 906 657 1449 2219 Fragments arranged by size: 2219 1449 906 657 617 BsaAI yAC'GTr Cuts at: 493 1204 2687 3639 493 Size: 711 1483 952 2702 Fragments arranged by size: 2702 1483 952 711 BsaBI GATnn'nnATC Cuts at: 5842 5842 Size: 5848 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3655 4037 4672 275 Size: 53 83 186 936 2122 382 635 1451 Fragments arranged by size: 2122 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4281 4679 4953 5100 5511 4281 Size: 398 274 147 411 4618 Fragments arranged by size: 4618 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2493 2516 2523 2546 1178 Size: 30 165 30 1090 23 7 23 4480 Fragments arranged by size: 4480 1090 165 30 30 23 23 7 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2150 2477 2738 2741 2880 3010 1186 Size: 964 327 261 3 139 130 4024 Fragments arranged by size: 4024 964 327 261 139 130 3 BseYI C'CCAG_C Cuts at: 2192 2433 3079 5051 5547 2192 Size: 241 646 1972 496 2493 Fragments arranged by size: 2493 1972 646 496 241 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2195 1163 Size: 573 459 4816 Fragments arranged by size: 4816 573 459 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3298 4059 5087 1540 Size: 275 186 1297 761 1028 2301 Fragments arranged by size: 2301 1297 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2859 3909 3994 5065 2859 Size: 1050 85 1071 3642 Fragments arranged by size: 3642 1071 1050 85 BsmI GAATG_Cn' Cuts at: 3359 3452 3359 Size: 93 5755 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2152 2227 2333 2699 2755 2847 2859 Size: 399 362 75 106 366 56 92 12 Cuts at: 2859 2907 2969 2992 3167 3909 3994 5065 1391 Size: 48 62 23 175 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 366 362 175 106 92 85 75 62 56 48 23 12 BspEI T'CCGG_A Cuts at: 5511 5511 Size: 5848 BspHI T'CATG_A Cuts at: 3737 5467 3737 Size: 1730 4118 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3007 3203 844 Size: 2163 196 3489 Fragments arranged by size: 3489 2163 196 BsrBI CCG'CTC Cuts at: 1394 1823 2491 2870 3735 1394 Size: 429 668 379 865 3507 Fragments arranged by size: 3507 865 668 429 379 BsrDI GCAATG_nn' Cuts at: 65 1248 4343 4517 65 Size: 1183 3095 174 1396 Fragments arranged by size: 3095 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2058 4492 4679 1700 Size: 110 248 2434 187 2869 Fragments arranged by size: 2869 2434 248 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5848 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5848 BssSI C'ACGA_G Cuts at: 3908 4920 3908 Size: 1012 4836 BstAPI GCAn_nnn'nTGC Cuts at: 794 2312 794 Size: 1518 4330 BstBI TT'CG_AA Cuts at: 4652 4652 Size: 5848 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5848 BstYI r'GATC_y Cuts at: 1972 2669 3178 3545 3930 3947 5399 5485 5497 Size: 697 509 367 385 17 1452 86 12 Cuts at: 5497 5843 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 697 509 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2073 2293 2385 2940 513 Size: 552 1008 220 92 555 3421 Fragments arranged by size: 3421 1008 555 552 220 92 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5109 Fragments arranged by size: 5109 683 56 BtsI GCAGTG_nn' Cuts at: 2207 2306 3105 3364 4158 4178 2207 Size: 99 799 259 794 20 3877 Fragments arranged by size: 3877 799 794 259 99 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5848 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5813 DraI TTT'AAA Cuts at: 3152 3152 Size: 5848 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4855 809 Size: 507 3539 1802 Fragments arranged by size: 3539 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2419 2958 3295 Size: 54 1425 50 275 18 589 539 337 Cuts at: 3295 4184 5813 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 589 539 337 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3295 1537 Size: 275 1483 4090 Fragments arranged by size: 4090 1483 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2454 2744 2763 2847 3778 1353 Size: 1101 290 19 84 931 3423 Fragments arranged by size: 3423 1101 931 290 84 19 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2371 4435 4809 4955 387 Size: 1064 138 782 2064 374 146 1280 Fragments arranged by size: 2064 1280 1064 782 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2147 2478 2495 2543 2683 2725 3911 Size: 393 515 331 17 48 140 42 1186 Cuts at: 3911 4508 5295 5563 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1186 787 597 515 393 331 268 140 48 42 17 EcoNI CCTnn'n_nnAGG Cuts at: 2922 3113 2922 Size: 191 5657 EcoO109I rG'GnC_Cy Cuts at: 2155 2329 2438 2929 2965 2966 3118 3139 2155 Size: 174 109 491 36 1 152 21 4864 Fragments arranged by size: 4864 491 174 152 109 36 21 1 EcoRI G'AATT_C Cuts at: 3157 3535 3157 Size: 378 5470 FspI TGC'GCA Cuts at: 4354 4354 Size: 5848 HaeII r_GCGC'y Cuts at: 1678 4995 1678 Size: 3317 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2127 2159 2465 2493 2497 Size: 32 135 32 555 32 306 28 4 Cuts at: 2497 2525 4489 4521 4563 4595 1373 Size: 28 1964 32 42 32 2626 Fragments arranged by size: 2626 1964 555 306 135 42 32 32 32 32 32 28 28 4 HincII GTy'rAC Cuts at: 669 1657 1938 2678 3192 3438 669 Size: 988 281 740 514 246 3079 Fragments arranged by size: 3079 988 740 514 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5848 HpaI GTT'AAC Cuts at: 3438 3438 Size: 5848 KpnI G_GTAC'C Cuts at: 3173 3173 Size: 5848 MfeI C'AATT_G Cuts at: 3447 3447 Size: 5848 MluI A'CGCG_T Cuts at: 5724 5724 Size: 5848 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4962 5146 1305 Size: 3657 184 2007 Fragments arranged by size: 3657 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2421 2960 10 Size: 54 1425 343 589 539 2898 Fragments arranged by size: 2898 1425 589 539 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3806 4165 4324 518 Size: 648 2640 359 159 2042 Fragments arranged by size: 2640 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2362 2373 2734 3024 3264 3941 5089 5334 Size: 756 11 361 290 240 677 1148 245 Cuts at: 5334 1606 Size: 2120 Fragments arranged by size: 2120 1148 756 677 361 290 245 240 11 NaeI GCC'GGC Cuts at: 2060 2060 Size: 5848 NcoI C'CATG_G Cuts at: 513 1065 2073 2293 2385 2940 513 Size: 552 1008 220 92 555 3421 Fragments arranged by size: 3421 1008 555 552 220 92 NdeI CA'TA_TG Cuts at: 387 387 Size: 5848 NgoMIV G'CCGG_C Cuts at: 2058 2058 Size: 5848 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5848 NotI GC'GGCC_GC Cuts at: 3295 3295 Size: 5848 NspI r_CATG'y Cuts at: 1132 1229 2315 2633 3206 4751 1132 Size: 97 1086 318 573 1545 2229 Fragments arranged by size: 2229 1545 1086 573 318 97 PasI CC'CwG_GG Cuts at: 1348 2445 2847 2918 1348 Size: 1097 402 71 4278 Fragments arranged by size: 4278 1097 402 71 PciI A'CATG_T Cuts at: 4747 4747 Size: 5848 PflMI CCAn_nnn'nTGG Cuts at: 1108 2428 1108 Size: 1320 4528 PmeI GTTT'AAAC Cuts at: 3152 3152 Size: 5848 PmlI CAC'GTG Cuts at: 2687 2687 Size: 5848 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3917 3949 5430 5462 3917 Size: 32 1481 32 4303 Fragments arranged by size: 4303 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2438 2929 2438 Size: 491 5357 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5848 PsiI TTA'TAA Cuts at: 3418 3418 Size: 5848 PspOMI G'GGCC_C Cuts at: 2148 2329 2843 2965 2148 Size: 181 514 122 5031 Fragments arranged by size: 5031 514 181 122 PstI C_TGCA'G Cuts at: 830 2134 2950 3048 3200 830 Size: 1304 816 98 152 3478 Fragments arranged by size: 3478 1304 816 152 98 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5848 SalI G'TCGA_C Cuts at: 1936 2676 3190 1936 Size: 740 514 4594 Fragments arranged by size: 4594 740 514 SanDI GG'GwC_CC Cuts at: 2438 2929 2438 Size: 491 5357 SapI GCTCTTCn'nnn_ Cuts at: 2744 2763 2847 2744 Size: 19 84 5745 Fragments arranged by size: 5745 84 19 SbfI CC_TGCA'GG Cuts at: 3200 3200 Size: 5848 ScaI AGT'ACT Cuts at: 4096 5808 4096 Size: 1712 4136 SfcI C'TryA_G Cuts at: 826 1045 2130 2946 3044 3196 4331 5012 5203 Size: 219 1085 816 98 152 1135 681 191 Cuts at: 5203 826 Size: 1471 Fragments arranged by size: 1471 1135 1085 816 681 219 191 152 98 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5848 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5848 SmaI CCC'GGG Cuts at: 1872 2624 2756 3175 1872 Size: 752 132 419 4545 Fragments arranged by size: 4545 752 419 132 SmlI C'TyrA_G Cuts at: 820 1017 1943 3004 3143 3952 4853 5115 820 Size: 197 926 1061 139 809 901 262 1553 Fragments arranged by size: 1553 1061 926 901 809 262 197 139 SnaBI TAC'GTA Cuts at: 493 3639 493 Size: 3146 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5848 SphI G_CATG'C Cuts at: 1132 2315 3206 1132 Size: 1183 891 3774 Fragments arranged by size: 3774 1183 891 SrfI GCCC'GGGC Cuts at: 2756 2756 Size: 5848 SspI AAT'ATT Cuts at: 5 52 3772 5 Size: 47 3720 2081 Fragments arranged by size: 3720 2081 47 StuI AGG'CCT Cuts at: 1517 2178 1517 Size: 661 5187 StyI C'CwwG_G Cuts at: 513 1065 2073 2293 2385 2484 2760 2940 3287 Size: 552 1008 220 92 99 276 180 347 Cuts at: 3287 513 Size: 3074 Fragments arranged by size: 3074 1008 552 347 276 220 180 99 92 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2187 2832 3875 3892 4045 4230 4675 Size: 1255 607 645 1043 17 153 185 445 Cuts at: 4675 325 Size: 1498 Fragments arranged by size: 1498 1255 1043 645 607 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4094 5806 96 Size: 274 80 33 51 1443 2117 1712 138 Fragments arranged by size: 2117 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2516 2548 5808 5840 2516 Size: 32 3260 32 2524 Fragments arranged by size: 3260 2524 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5848 XbaI T'CTAG_A Cuts at: 3184 3184 Size: 5848 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2071 2291 2071 Size: 220 5628 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5848 XmaI C'CCGG_G Cuts at: 1870 2622 2754 3173 1870 Size: 752 132 419 4545 Fragments arranged by size: 4545 752 419 132 XmnI GAAnn'nnTTC Cuts at: 3977 3977 Size: 5848 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3656 276 Size: 53 83 186 936 2122 2468 Fragments arranged by size: 2468 2122 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PspXI PsrI PvuII RsrII SacI SacII SexAI SfiI SfoI SwaI