Restriction map for
pFN21AB7630
##################################
# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BcgI BclI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmAI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRI
EcoRV FbaI FseI FspI Hin1I
HindIII HpaI KasI KspI MamI
MfeI MflI MluI MluNI MroI
MscI MspA1I MunI NaeI NarI
NcoI NgoMIV NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SwaI
TfiI Tth111I XbaI XcmI XhoII
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 891 GGTACC G^GTACC
AccII 691 CGCG CG^CG
AflII 319 CTTAAG C^TTAAG
AflIII 757 ACACGT A^C[GA][CT]GT
AlwNI 1053 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 18 GGGCCC GGGCC^C
Asp718I 891 GGTACC G^GTACC
AvaI 637 CTCGAG C^[CT]CG[GA]G
BanI 891 GGTACC G^G[CT][GA]CC
BbvCI 681 CCTCAGC CCTCAGC(-5/-2)
BciVI 33 GTATCC GTATCC(6/5)
BcnI 968 CCCGG CC^[GC]GG
BfaI 866 CTAG C^TAG
BfrI 319 CTTAAG C^TTAAG
BseRI 139 GAGGAG GAGGAG(10/8)
BsgI 1037 CTGCAC GTGCAG(16/14)
BsoBI 637 CTCGAG C^[CT]CG[GA]G
BspMI 706 ACCTGC ACCTGC(4/8)
BsrFI 431 GCCGGT [GA]^CCGG[CT]
BstAPI 739 GCACCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 384 GGTGACC G^GT[ACGT]ACC
BstPI 384 GGTGACC G^GT[ACGT]ACC
BstUI 691 CGCG CG^CG
BstXI 920 CCACCCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 431 GCCGGT [GA]^CCGG[CT]
DraIII 582 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 249 TGGCCG [CT]^GGCC[GA]
EarI 855 GAAGAG CTCTTC(1/4)
EcoO65I 384 GGTGACC G^GT[ACGT]ACC
EcoT14I 329 CCAAGG C^C[AT][AT]GG
HaeII 1045 GGCGCT [GA]GCGC^[CT]
HgaI 991 GACGC GACGC(5/10)
HincII 201 GTTGAC GT[CT]^[GA]AC
HindII 201 GTTGAC GT[CT]^[GA]AC
HinfI 623 GACTC G^A[ACGT]TC
KpnI 891 GGTACC GGTAC^C
Ksp632I 855 GAAGAG CTCTTC(1/4)
MaeI 866 CTAG C^TAG
MvnI 691 CGCG CG^CG
NciI 968 CCCGG CC^[GC]GG
NdeI 77 CATATG CA^TATG
NheI 865 GCTAGC G^CTAGC
NspI 537 GCATGC [GA]CATG^[CT]
PaeR7I 637 CTCGAG C^TCGAG
PleI 623 GACTC GAGTC(4/5)
SapI 855 GAAGAGC GCTCTTC(1/4)
SphI 537 GCATGC GCATG^C
SspI 102 AATATT AAT^ATT
StuI 1115 AGGCCT AGG^CCT
StyI 329 CCAAGG C^C[AT][AT]GG
Tsp509I 601 AATT ^AATT
TspRI 1040 CACTG CA[GC]TG[ACGT][ACGT]^
XhoI 637 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 261 GATCC GGATC(4/5)
561 GGATC
AvaII 229 GGACC G^G[AT]CC
254 GGACC
BsmFI 944 GGGAC GGGAC(10/14)
357 GTCCC
BsmI 166 GCATTC GAATGC(1/-1)
337 GCATTC
CfoI 729 GCGC GCG^C
1046 GCGC
DdeI 176 CTGAG C^T[ACGT]AG
682 CTCAG
DpnII 261 GATC ^GATC
562 GATC
EcoT22I 164 ATGCAT ATGCA^T
535 ATGCAT
HhaI 729 GCGC GCG^C
1046 GCGC
HinP1I 729 GCGC G^CGC
1046 GCGC
MboI 261 GATC ^GATC
562 GATC
MslI 411 CACCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
921 CACCCTGATG
NdeII 261 GATC ^GATC
562 GATC
NsiI 164 ATGCAT ATGCA^T
535 ATGCAT
PflMI 55 CCAGGGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
552 CCAGATGTTGG
Sau3AI 261 GATC ^GATC
562 GATC
SfcI 96 CTACAG C^T[GA][CT]AG
675 CTACAG
TaqI 362 TCGA T^CGA
638 TCGA
TthHB8I 362 TCGA T^CGA
638 TCGA
Van91I 55 CCAGGGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
552 CCAGATGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AfaI AluI AspHI BanII
BbvI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HpaII HphI ItaI MaeII MaeIII
MboII MnlI MseI MspI MvaI
MwoI NlaIII NlaIV RsaI SacI
Sau96I ScrFI SfaNI SmlI Tru9I
TseI Tsp45I