Restriction map for
pFN21AB7896
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspI AvaI AviII
AvrII BalI BanI BbeI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BsaBI BsiEI BsiWI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrGI BssHII Bst1107I BstBI
BstZ17I Bsu36I ClaI CpoI DraI
DrdI DsaI EaeI EagI EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoRV FauI FbaI FseI
FspI HaeII HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MamI MfeI MluI MluNI MscI
MunI NaeI NarI NcoI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
Van91I XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 16 CGCG CG^CG
AccIII 752 TCCGGA T^CCGGA
AcyI 459 GACGCC G[GA]^CG[CT]C
AflIII 574 ACACGT A^C[GA][CT]GT
AhdI 269 GACCCAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 269 GACCCAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII 1683 GAGCTC G[GA]GC[CT]^C
BbrPI 575 CACGTG CAC^GTG
BbsI 456 GAAGAC GAAGAC(2/6)
BcnI 30 CCCGG CC^[GC]GG
BpuAI 456 GAAGAC GAAGAC(2/6)
BsaAI 575 CACGTG [CT]AC^GT[GA]
BsaHI 459 GACGCC G[GA]^CG[CT]C
BseAI 752 TCCGGA T^CCGGA
BsmBI 18 CGTCTC CGTCTC(1/5)
BspEI 752 TCCGGA T^CCGGA
BsrBI 1369 GAGCGG CCGCTC(-3/-3)
BsrFI 435 GCCGGT [GA]^CCGG[CT]
BssSI 946 CACGAG CACGAG(-5/-1)
BstAPI 1487 GCAATGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 167 GGTTACC G^GT[ACGT]ACC
BstPI 167 GGTTACC G^GT[ACGT]ACC
BstUI 16 CGCG CG^CG
BstXI 1152 CCATGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 15 GCGC GCG^C
Cfr10I 435 GCCGGT [GA]^CCGG[CT]
DraIII 816 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 269 GACCCAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 167 GGTTACC G^GT[ACGT]ACC
EcoT22I 1901 ATGCAT ATGCA^T
HhaI 15 GCGC GCG^C
Hin1I 459 GACGCC G[GA]^CG[CT]C
HinP1I 15 GCGC G^CGC
MroI 752 TCCGGA T^CCGGA
MseI 742 TTAA T^TAA
MspA1I 88 CAGCTG C[AC]G^C[GT]G
MvnI 16 CGCG CG^CG
NciI 30 CCCGG CC^[GC]GG
NdeI 1898 CATATG CA^TATG
NsiI 1901 ATGCAT ATGCA^T
NspI 1903 GCATGC [GA]CATG^[CT]
PmaCI 575 CACGTG CAC^GTG
PmlI 575 CACGTG CAC^GTG
PstI 369 CTGCAG CTGCA^G
PvuII 88 CAGCTG CAG^CTG
SacI 1683 GAGCTC GAGCT^C
SfcI 369 CTGCAG C^T[GA][CT]AG
SphI 1903 GCATGC GCATG^C
Tru9I 742 TTAA T^TAA
Tsp509I 843 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwNI 1027 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
1908 CAGGGACTG
BamHI 1625 GGATCC G^GATCC
1877 GGATCC
BbvI 87 GCAGC GCAGC(8/12)
899 GCAGC
BlpI 677 GCTCAGC GC^T[ACGT]AGC
1776 GCTCAGC
BpmI 196 CTGGAG CTGGAG(16/14)
469 CTGGAG
Bpu1102I 677 GCTCAGC GC^T[ACGT]AGC
1776 GCTCAGC
BsaI 717 GAGACC GGTCTC(1/5)
150 GGTCTC
BsgI 981 GTGCAG GTGCAG(16/14)
1401 GTGCAG
BsrDI 1487 GCAATG GCAATG(2/0)
1739 GCAATG
BstYI 1625 GGATCC [GA]^GATC[CT]
1877 GGATCC
CelII 677 GCTCAGC GC^T[ACGT]AGC
1776 GCTCAGC
EcoT14I 240 CCAAGG C^C[AT][AT]GG
1718 CCAAGG
MboII 310 GAAGA GAAGA(8/7)
456 GAAGA
MflI 1625 GGATCC [GA]^GATC[CT]
1877 GGATCC
StyI 240 CCAAGG C^C[AT][AT]GG
1718 CCAAGG
TaqI 689 TCGA T^CGA
1697 TCGA
TseI 87 GCAGC G^C[AT]GC
899 GCAGC
TthHB8I 689 TCGA T^CGA
1697 TCGA
XbaI 229 TCTAGA T^CTAGA
417 TCTAGA
XhoII 1625 GGATCC [GA]^GATC[CT]
1877 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AfaI AluI AlwI Asp700I
AspHI AvaII BfaI BmyI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MnlI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI StuI TfiI
Tsp45I TspRI XmnI