Restriction map for
pFN21AB7896
(Circular) (Six-base) MAPSORT of: pFN21AB7896.seq Check: 631 from: 1 to: 6679
pFN21AB7896 6679 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4489 278
Size: 53 83 186 936 2953 2468
Fragments arranged by size:
2953 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4022 1937
Size: 2085 4594
Acc65I G'GTAC_C
Cuts at: 4000 4000
Size: 6679
AclI AA'CG_TT
Cuts at: 1563 4806 5179 1563
Size: 3243 373 3063
Fragments arranged by size:
3243 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2056 2117 2128 2334 2908 2926 3076 3244
Size: 424 61 11 206 574 18 150 168
Cuts at: 3244 3328 3580 3664 3748 4742 6126 6394 1632
Size: 84 252 84 84 994 1384 268 1917
Fragments arranged by size:
1917 1384 994 574 424 268 252 206
168 150 84 84 84 61 18 11
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6482
AflIII A'CryG_T
Cuts at: 1203 2579 5578 6555 1203
Size: 1376 2999 977 1327
Fragments arranged by size:
2999 1376 1327 977
AgeI A'CCGG_T
Cuts at: 5510 5510
Size: 6679
AhdI GACnn_n'nnGTC
Cuts at: 2279 5408 2279
Size: 3129 3550
AleI CACnn'nnGTG
Cuts at: 1166 2456 2883 1166
Size: 1290 427 4962
Fragments arranged by size:
4962 1290 427
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6679
AlwNI CAG_nnn'CTG
Cuts at: 3037 3918 4422 5994 3037
Size: 881 504 1572 3722
Fragments arranged by size:
3722 1572 881 504
ApaLI G'TGCA_C
Cuts at: 4736 5892 4736
Size: 1156 5523
ApoI r'AATT_y
Cuts at: 1493 3988 4214 4366 6475 1493
Size: 2495 226 152 2109 1697
Fragments arranged by size:
2495 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5233 160
Size: 872 4201 1606
Fragments arranged by size:
4201 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6679
AvaI C'yCGr_G
Cuts at: 1870 1943 4004 1870
Size: 73 2061 4545
Fragments arranged by size:
4545 2061 73
BamHI G'GATC_C
Cuts at: 3630 3882 4009 4376 3630
Size: 252 127 367 5933
Fragments arranged by size:
5933 367 252 127
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4000 5455 618
Size: 325 217 627 2213 1455 1842
Fragments arranged by size:
2213 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3692 3998 1391
Size: 2301 306 4072
Fragments arranged by size:
4072 2301 306
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2468 928
Size: 971 569 5139
Fragments arranged by size:
5139 971 569
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4870 4904 1903
Size: 34 2933 34 3678
Fragments arranged by size:
3678 2933 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4571 5781 4571
Size: 1210 5469
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6679
BfrBI ATG'CAT
Cuts at: 3908 3908
Size: 6679
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5290 136
Size: 107 122 71 631 605 3618 1525
Fragments arranged by size:
3618 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6674 6674
Size: 6679
BlpI GC'TnA_GC
Cuts at: 2683 3782 4096 2683
Size: 1099 314 5266
Fragments arranged by size:
5266 1099 314
Bme1580I G_kGCm'C
Cuts at: 1790 4740 5896 1790
Size: 2950 1156 2573
Fragments arranged by size:
2950 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2059 1318
Size: 741 5938
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2410 2808 3060
Size: 416 53 83 399 24 993 398 252
Cuts at: 3060 3564 3816 4410 5368 442
Size: 504 252 594 958 1753
Fragments arranged by size:
1753 993 958 594 504 416 399 398
252 252 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6679
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2222 2495 3996 5339 1239
Size: 983 273 1501 1343 2579
Fragments arranged by size:
2579 1501 1343 983 273
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4804 5669 5967 4804
Size: 865 298 5516
Fragments arranged by size:
5516 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2161 2716 5342 882
Size: 617 662 555 2626 2219
Fragments arranged by size:
2626 2219 662 617 555
BsaAI yAC'GTr
Cuts at: 493 1204 2582 4470 493
Size: 711 1378 1888 2702
Fragments arranged by size:
2702 1888 1378 711
BsaBI GATnn'nnATC
Cuts at: 6673 6673
Size: 6679
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2465 4486 4868 5503
Size: 53 83 186 936 932 2021 382 635
Cuts at: 5503 275
Size: 1451
Fragments arranged by size:
2021 1451 936 932 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2703 2757 3876 5112 5510 5784 5931 6342 2703
Size: 54 1119 1236 398 274 147 411 3040
Fragments arranged by size:
3040 1236 1119 411 398 274 147 54
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3929 3959 1178
Size: 30 165 30 2526 30 3898
Fragments arranged by size:
3898 2526 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2096 3346 3766 3934 1186
Size: 910 1250 420 168 3931
Fragments arranged by size:
3931 1250 910 420 168
BseYI C'CCAG_C
Cuts at: 3030 5882 6378 3030
Size: 2852 496 3331
Fragments arranged by size:
3331 2852 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3007 3427 1163
Size: 573 1271 420 4415
Fragments arranged by size:
4415 1271 573 420
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4129 4890 5918 1540
Size: 275 186 2128 761 1028 2301
Fragments arranged by size:
2301 2128 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3272 3440 3692 3860 4740 4825 5896 3272
Size: 168 252 168 880 85 1071 4055
Fragments arranged by size:
4055 1071 880 252 168 168 85
BsmI GAATG_Cn'
Cuts at: 2663 2747 2906 3494 3509 3578 4190 4283 2663
Size: 84 159 588 15 69 612 93 5059
Fragments arranged by size:
5059 612 588 159 93 84 69 15
BsmBI CGTCTCn'nnnn_
Cuts at: 2029 2029
Size: 6679
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3272 3440 3692 3860 3998 4740 4825
Size: 399 1482 168 252 168 138 742 85
Cuts at: 4825 5896 1391
Size: 1071 2174
Fragments arranged by size:
2174 1482 1071 742 399 252 168 168
138 85
BspEI T'CCGG_A
Cuts at: 2757 6342 2757
Size: 3585 3094
BspHI T'CATG_A
Cuts at: 4568 6298 4568
Size: 1730 4949
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4034 844
Size: 3190 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3376 4566 1394
Size: 429 1553 1190 3507
Fragments arranged by size:
3507 1553 1190 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3499 3751 5174 5348 65
Size: 1183 2251 252 1423 174 1396
Fragments arranged by size:
2251 1423 1396 1183 252 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2440 5323 5510 1700
Size: 110 630 2883 187 2869
Fragments arranged by size:
2883 2869 630 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6679
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6679
BssSI C'ACGA_G
Cuts at: 2951 4739 5751 2951
Size: 1788 1012 3879
Fragments arranged by size:
3879 1788 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3498 794
Size: 2704 3975
BstBI TT'CG_AA
Cuts at: 5483 5483
Size: 6679
BstEII G'GTnAC_C
Cuts at: 1184 2172 1184
Size: 988 5691
BstXI CCAn_nnnn'nTGG
Cuts at: 3164 3164
Size: 6679
BstYI r'GATC_y
Cuts at: 1972 3630 3882 4009 4376 4761 4778 6230 6316
Size: 1658 252 127 367 385 17 1452 86
Cuts at: 6316 6328 6674 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1658 1452 385 367 346 252 127
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6127
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5940
Fragments arranged by size:
5940 683 56
BtsI GCAGTG_nn'
Cuts at: 2442 2486 2995 4195 4989 5009 2442
Size: 44 509 1200 794 20 4112
Fragments arranged by size:
4112 1200 794 509 44 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6679
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3313 3348 3565 3600 3733 3768 529
Size: 35 2749 35 217 35 133 35 3440
Fragments arranged by size:
3440 2749 217 133 35 35 35 35
DraI TTT'AAA
Cuts at: 3983 3983
Size: 6679
DraIII CAC_nnn'GTG
Cuts at: 2826 2826
Size: 6679
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5686 809
Size: 507 4370 1802
Fragments arranged by size:
4370 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4126 5015 6644
Size: 54 1425 50 275 18 2296 889 1629
Cuts at: 6644 8
Size: 43
Fragments arranged by size:
2296 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4126 1537
Size: 275 2314 4090
Fragments arranged by size:
4090 2314 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4609 1353
Size: 3256 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5266 5640 5786 387
Size: 1064 138 3677 374 146 1280
Fragments arranged by size:
3677 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3690 3690
Size: 6679
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2056 2117 2128 2222 2334 2495 2908
Size: 393 424 61 11 94 112 161 413
Cuts at: 2908 2926 3076 3244 3328 3580 3664 3748 3996
Size: 18 150 168 84 252 84 84 248
Cuts at: 3996 4742 5339 6126 6394 1239
Size: 746 597 787 268 1524
Fragments arranged by size:
1524 787 746 597 424 413 393 268
252 248 168 161 150 112 94 84
84 84 61 18 11
EcoO109I rG'GnC_Cy
Cuts at: 2112 2835 2934 3210 2112
Size: 723 99 276 5581
Fragments arranged by size:
5581 723 276 99
EcoRI G'AATT_C
Cuts at: 3988 4366 3988
Size: 378 6301
FspI TGC'GCA
Cuts at: 5185 5185
Size: 6679
HaeII r_GCGC'y
Cuts at: 1678 5826 1678
Size: 4148 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2265 2297 3271 3303 5320
Size: 32 135 32 693 32 974 32 2017
Cuts at: 5320 5352 5394 5426 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2017 974 693 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4023 4269 669
Size: 988 281 2085 246 3079
Fragments arranged by size:
3079 2085 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6679
HpaI GTT'AAC
Cuts at: 4269 4269
Size: 6679
KpnI G_GTAC'C
Cuts at: 4004 4004
Size: 6679
MfeI C'AATT_G
Cuts at: 4278 4278
Size: 6679
MluI A'CGCG_T
Cuts at: 6555 6555
Size: 6679
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2349 5793 5977 1305
Size: 1044 3444 184 2007
Fragments arranged by size:
3444 2007 1044 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4857
Fragments arranged by size:
4857 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2456 2883 3162 3907 4637 4996 5155
Size: 648 1290 427 279 745 730 359 159
Cuts at: 5155 518
Size: 2042
Fragments arranged by size:
2042 1290 745 730 648 427 359 279
159
MspA1I CmG'CkG
Cuts at: 1606 2095 4095 4772 5920 6165 1606
Size: 489 2000 677 1148 245 2120
Fragments arranged by size:
2120 2000 1148 677 489 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6127
NdeI CA'TA_TG
Cuts at: 387 3904 387
Size: 3517 3162
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6679
NotI GC'GGCC_GC
Cuts at: 4126 4126
Size: 6679
NsiI A_TGCA'T
Cuts at: 3910 3910
Size: 6679
NspI r_CATG'y
Cuts at: 1132 1229 3912 4037 5582 1132
Size: 97 2683 125 1545 2229
Fragments arranged by size:
2683 2229 1545 125 97
PasI CC'CwG_GG
Cuts at: 1348 2012 1348
Size: 664 6015
PciI A'CATG_T
Cuts at: 5578 5578
Size: 6679
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6679
PmeI GTTT'AAAC
Cuts at: 3983 3983
Size: 6679
PmlI CAC'GTG
Cuts at: 2582 2582
Size: 6679
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4748 4780 6261 6293 4748
Size: 32 1481 32 5134
Fragments arranged by size:
5134 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2112 2835 2934 3210 2112
Size: 723 99 276 5581
Fragments arranged by size:
5581 723 276 99
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6679
PsiI TTA'TAA
Cuts at: 4249 4249
Size: 6679
PstI C_TGCA'G
Cuts at: 830 2378 4031 830
Size: 1548 1653 3478
Fragments arranged by size:
3478 1653 1548
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6679
PvuII CAG'CTG
Cuts at: 2095 2095
Size: 6679
SacI G_AGCT'C
Cuts at: 3692 3692
Size: 6679
SalI G'TCGA_C
Cuts at: 1936 4021 1936
Size: 2085 4594
SbfI CC_TGCA'GG
Cuts at: 4031 4031
Size: 6679
ScaI AGT'ACT
Cuts at: 4927 6639 4927
Size: 1712 4967
SfcI C'TryA_G
Cuts at: 826 1045 2374 4027 5162 5843 6034 826
Size: 219 1329 1653 1135 681 191 1471
Fragments arranged by size:
1653 1471 1329 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6679
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6679
SmaI CCC'GGG
Cuts at: 1872 4006 1872
Size: 2134 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4783 5684 5946 820
Size: 197 926 2840 901 262 1553
Fragments arranged by size:
2840 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4470 493
Size: 3977 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6679
SphI G_CATG'C
Cuts at: 1132 3912 4037 1132
Size: 2780 125 3774
Fragments arranged by size:
3774 2780 125
SspI AAT'ATT
Cuts at: 5 52 4603 5
Size: 47 4551 2081
Fragments arranged by size:
4551 2081 47
StuI AGG'CCT
Cuts at: 1517 2669 3089 3425 3929 1517
Size: 1152 420 336 504 4267
Fragments arranged by size:
4267 1152 504 420 336
StyI C'CwwG_G
Cuts at: 513 1065 2245 3723 4118 513
Size: 552 1180 1478 395 3074
Fragments arranged by size:
3074 1478 1180 552 395
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3044 3194 3296 3464 3729 3875 4706
Size: 1255 1464 150 102 168 265 146 831
Cuts at: 4706 4723 4876 5061 5506 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1464 1255 831 445 265 185 168
153 150 146 102 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4925 6637 96
Size: 274 80 33 51 1443 2948 1712 138
Fragments arranged by size:
2948 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2351 2383 3089 3121 3509 3541 3929 3961 6639
Size: 32 706 32 388 32 388 32 2678
Cuts at: 6639 6671 2351
Size: 32 2359
Fragments arranged by size:
2678 2359 706 388 388 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6679
XbaI T'CTAG_A
Cuts at: 2234 2422 4015 2234
Size: 188 1593 4898
Fragments arranged by size:
4898 1593 188
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6679
XmaI C'CCGG_G
Cuts at: 1870 4004 1870
Size: 2134 4545
XmnI GAAnn'nnTTC
Cuts at: 2921 3005 3257 3341 3425 3761 4808 2921
Size: 84 252 84 84 336 1047 4792
Fragments arranged by size:
4792 1047 336 252 84 84 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4487 276
Size: 53 83 186 936 2953 2468
Fragments arranged by size:
2953 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI