Restriction map for
pFN21AB7896
(Linear) (Six-base) MAPSORT of: KIBB7896.seq Check: 8008 from: 1 to: 1974
KIBB7896.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 112 123 329 903 921 1071 1239
Size: 51 61 11 206 574 18 150 168
Cuts at: 1239 1323 1575 1659 1743 1974
Size: 84 252 84 84 231
Fragments arranged by size:
574 252 231 206 168 150 84 84
84 61 51 18 11
AflIII A'CryG_T
Cuts at: 0 574 1974
Size: 574 1400
AhdI GACnn_n'nnGTC
Cuts at: 0 274 1974
Size: 274 1700
AleI CACnn'nnGTG
Cuts at: 0 451 878 1974
Size: 451 427 1096
Fragments arranged by size:
1096 451 427
AlwNI CAG_nnn'CTG
Cuts at: 0 1032 1913 1974
Size: 1032 881 61
Fragments arranged by size:
1032 881 61
BamHI G'GATC_C
Cuts at: 0 1625 1877 1974
Size: 1625 252 97
Fragments arranged by size:
1625 252 97
BanII G_rGCy'C
Cuts at: 0 1687 1974
Size: 1687 287
BbsI GAAGACnn'nnnn_
Cuts at: 0 463 1974
Size: 463 1511
BfrBI ATG'CAT
Cuts at: 0 1903 1974
Size: 1903 71
BlpI GC'TnA_GC
Cuts at: 0 678 1777 1974
Size: 678 1099 197
Fragments arranged by size:
1099 678 197
BmgBI CAC'GTC
Cuts at: 0 54 1974
Size: 54 1920
BmrI ACTGGGnnnn_n'
Cuts at: 0 405 803 1055 1559 1811 1974
Size: 405 398 252 504 252 163
Fragments arranged by size:
504 405 398 252 252 163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 490 1974
Size: 217 273 1484
Fragments arranged by size:
1484 273 217
BsaI GGTCTCn'nnnn_
Cuts at: 0 156 711 1974
Size: 156 555 1263
Fragments arranged by size:
1263 555 156
BsaAI yAC'GTr
Cuts at: 0 577 1974
Size: 577 1397
BsaHI Gr'CG_yC
Cuts at: 0 460 1974
Size: 460 1514
BsaWI w'CCGG_w
Cuts at: 0 698 752 1871 1974
Size: 698 54 1119 103
Fragments arranged by size:
1119 698 103 54
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1924 1954 1974
Size: 1924 30 20
Fragments arranged by size:
1924 30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 91 1341 1761 1929 1974
Size: 91 1250 420 168 45
Fragments arranged by size:
1250 420 168 91 45
BseYI C'CCAG_C
Cuts at: 0 1025 1974
Size: 1025 949
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1002 1422 1974
Size: 1002 420 552
Fragments arranged by size:
1002 552 420
BsiHKAI G_wGCw'C
Cuts at: 0 1267 1435 1687 1855 1974
Size: 1267 168 252 168 119
Fragments arranged by size:
1267 252 168 168 119
BsmI GAATG_Cn'
Cuts at: 0 658 742 901 1489 1504 1573 1974
Size: 658 84 159 588 15 69 401
Fragments arranged by size:
658 588 401 159 84 69 15
BsmBI CGTCTCn'nnnn_
Cuts at: 0 24 1974
Size: 24 1950
Bsp1286I G_dGCh'C
Cuts at: 0 1267 1435 1687 1855 1974
Size: 1267 168 252 168 119
Fragments arranged by size:
1267 252 168 168 119
BspEI T'CCGG_A
Cuts at: 0 752 1974
Size: 752 1222
BsrBI CCG'CTC
Cuts at: 0 1371 1974
Size: 1371 603
BsrDI GCAATG_nn'
Cuts at: 0 1494 1746 1974
Size: 1494 252 228
Fragments arranged by size:
1494 252 228
BsrFI r'CCGG_y
Cuts at: 0 435 1974
Size: 435 1539
BssSI C'ACGA_G
Cuts at: 0 946 1974
Size: 946 1028
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1493 1974
Size: 1493 481
BstEII G'GTnAC_C
Cuts at: 0 167 1974
Size: 167 1807
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1159 1974
Size: 1159 815
BstYI r'GATC_y
Cuts at: 0 1625 1877 1974
Size: 1625 252 97
Fragments arranged by size:
1625 252 97
BtsI GCAGTG_nn'
Cuts at: 0 437 481 990 1974
Size: 437 44 509 984
Fragments arranged by size:
984 509 437 44
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1308 1343 1560 1595 1728 1763 1974
Size: 1308 35 217 35 133 35 211
Fragments arranged by size:
1308 217 211 133 35 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 821 1974
Size: 821 1153
EcoICRI GAG'CTC
Cuts at: 0 1685 1974
Size: 1685 289
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 112 123 217 329 490 903 921
Size: 51 61 11 94 112 161 413 18
Cuts at: 921 1071 1239 1323 1575 1659 1743 1974
Size: 150 168 84 252 84 84 231
Fragments arranged by size:
413 252 231 168 161 150 112 94
84 84 84 61 51 18 11
EcoO109I rG'GnC_Cy
Cuts at: 0 107 830 929 1205 1974
Size: 107 723 99 276 769
Fragments arranged by size:
769 723 276 107 99
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 260 292 1266 1298 1974
Size: 260 32 974 32 676
Fragments arranged by size:
974 676 260 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 1974
Size: 344 1630
MslI CAynn'nnrTG
Cuts at: 0 451 878 1157 1902 1974
Size: 451 427 279 745 72
Fragments arranged by size:
745 451 427 279 72
MspA1I CmG'CkG
Cuts at: 0 90 1974
Size: 90 1884
NdeI CA'TA_TG
Cuts at: 0 1899 1974
Size: 1899 75
NsiI A_TGCA'T
Cuts at: 0 1905 1974
Size: 1905 69
NspI r_CATG'y
Cuts at: 0 1907 1974
Size: 1907 67
PasI CC'CwG_GG
Cuts at: 0 7 1974
Size: 7 1967
PmlI CAC'GTG
Cuts at: 0 577 1974
Size: 577 1397
PpuMI rG'GwC_Cy
Cuts at: 0 107 830 929 1205 1974
Size: 107 723 99 276 769
Fragments arranged by size:
769 723 276 107 99
PstI C_TGCA'G
Cuts at: 0 373 1974
Size: 373 1601
PvuII CAG'CTG
Cuts at: 0 90 1974
Size: 90 1884
SacI G_AGCT'C
Cuts at: 0 1687 1974
Size: 1687 287
SfcI C'TryA_G
Cuts at: 0 369 1974
Size: 369 1605
SphI G_CATG'C
Cuts at: 0 1907 1974
Size: 1907 67
StuI AGG'CCT
Cuts at: 0 664 1084 1420 1924 1974
Size: 664 420 336 504 50
Fragments arranged by size:
664 504 420 336 50
StyI C'CwwG_G
Cuts at: 0 240 1718 1974
Size: 240 1478 256
Fragments arranged by size:
1478 256 240
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1039 1189 1291 1459 1724 1870 1974
Size: 1039 150 102 168 265 146 104
Fragments arranged by size:
1039 265 168 150 146 104 102
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 346 378 1084 1116 1504 1536 1924 1956
Size: 346 32 706 32 388 32 388 32
Cuts at: 1956 1974
Size: 18
Fragments arranged by size:
706 388 388 346 32 32 32 32
18
XbaI T'CTAG_A
Cuts at: 0 229 417 1974
Size: 229 188 1557
Fragments arranged by size:
1557 229 188
XmnI GAAnn'nnTTC
Cuts at: 0 916 1000 1252 1336 1420 1756 1974
Size: 916 84 252 84 84 336 218
Fragments arranged by size:
916 336 252 218 84 84 84
Enzymes that do cut:
AcuI AflIII AhdI AleI AlwNI BamHI BanII BbsI
BfrBI BlpI BmgBI BmrI BpmI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BsrBI BsrDI BsrFI BssSI BstAPI BstEII
BstXI BstYI BtsI CspCI DraIII EcoICRI Eco57MI EcoO109I
Hin4I MmeI MslI MspA1I NdeI NsiI NspI PasI
PmlI PpuMI PstI PvuII SacI SfcI SphI StuI
StyI TaqII TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BanI BbeI BbvCI BcgI BciVI
BclI BglI BglII Bme1580I BmtI BplI Bpu10I BpuEI
BsaBI BsiEI BsiWI BspHI BspMI BsrGI BssHII BstBI
BstZ17I Bsu36I BtgI BtgZI ClaI DraI DrdI EaeI
EagI EarI EciI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NcoI NgoMIV NheI
NotI NruI PacI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI
SwaI TatI Tth111I XcmI XhoI XmaI ZraI