(Circular) (Six-base) MAPSORT of: pFN21AB8387.seq Check: 216 from: 1 to: 6409 pFN21AB8387 6409 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4219 278 Size: 53 83 186 936 2683 2468 Fragments arranged by size: 2683 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2505 3752 1937 Size: 568 1247 4594 Fragments arranged by size: 4594 1247 568 Acc65I G'GTAC_C Cuts at: 2180 2982 3730 2180 Size: 802 748 4859 Fragments arranged by size: 4859 802 748 AclI AA'CG_TT Cuts at: 1563 4536 4909 1563 Size: 2973 373 3063 Fragments arranged by size: 3063 2973 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2577 3090 3597 3657 3699 4472 5856 6124 Size: 945 513 507 60 42 773 1384 268 Cuts at: 6124 1632 Size: 1917 Fragments arranged by size: 1917 1384 945 773 513 507 268 60 42 AflII C'TTAA_G Cuts at: 820 1017 2312 2351 820 Size: 197 1295 39 4878 Fragments arranged by size: 4878 1295 197 39 AflIII A'CryG_T Cuts at: 1203 3688 5308 6285 1203 Size: 2485 1620 977 1327 Fragments arranged by size: 2485 1620 1327 977 AgeI A'CCGG_T Cuts at: 2786 5240 2786 Size: 2454 3955 AhdI GACnn_n'nnGTC Cuts at: 5138 5138 Size: 6409 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6409 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3247 1184 Size: 2063 4346 AlwNI CAG_nnn'CTG Cuts at: 4152 5724 4152 Size: 1572 4837 ApaLI G'TGCA_C Cuts at: 4466 5622 4466 Size: 1156 5253 ApoI r'AATT_y Cuts at: 1493 2122 2765 3555 3718 3944 4096 6205 1493 Size: 629 643 790 163 226 152 2109 1697 Fragments arranged by size: 2109 1697 790 643 629 226 163 152 AseI AT'TA_AT Cuts at: 160 1032 4963 160 Size: 872 3931 1606 Fragments arranged by size: 3931 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6409 AvaI C'yCGr_G Cuts at: 1870 1943 3117 3734 1870 Size: 73 1174 617 4545 Fragments arranged by size: 4545 1174 617 73 AvrII C'CTAG_G Cuts at: 3277 3277 Size: 6409 BamHI G'GATC_C Cuts at: 3739 4106 3739 Size: 367 6042 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2180 2982 3296 3730 5185 Size: 325 217 627 393 802 314 434 1455 Cuts at: 5185 618 Size: 1842 Fragments arranged by size: 1842 1455 802 627 434 393 325 314 217 BanII G_rGCy'C Cuts at: 1391 3728 1391 Size: 2337 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5438 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4600 4634 1903 Size: 34 2663 34 3678 Fragments arranged by size: 3678 2663 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3187 4301 5511 3187 Size: 1114 1210 4085 Fragments arranged by size: 4085 1210 1114 BclI T'GATC_A Cuts at: 1548 1548 Size: 6409 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2030 5020 136 Size: 107 122 71 631 605 358 2990 1525 Fragments arranged by size: 2990 1525 631 605 358 122 107 71 BglII A'GATC_T Cuts at: 3448 6404 3448 Size: 2956 3453 BlpI GC'TnA_GC Cuts at: 3826 3826 Size: 6409 Bme1580I G_kGCm'C Cuts at: 1790 3299 4470 5626 1790 Size: 1509 1171 1156 2573 Fragments arranged by size: 2573 1509 1171 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6409 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4140 5098 442 Size: 416 53 83 399 24 2723 958 1753 Fragments arranged by size: 2723 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6409 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2421 2665 3422 5069 1239 Size: 1182 244 757 1647 2579 Fragments arranged by size: 2579 1647 1182 757 244 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2530 3671 4534 5399 5697 2530 Size: 1141 863 865 298 3242 Fragments arranged by size: 3242 1141 865 863 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3319 3581 5072 882 Size: 617 1820 262 1491 2219 Fragments arranged by size: 2219 1820 1491 617 262 BsaAI yAC'GTr Cuts at: 493 1204 4200 493 Size: 711 2996 2702 Fragments arranged by size: 2996 2702 711 BsaBI GATnn'nnATC Cuts at: 6403 6403 Size: 6409 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4216 4598 5233 275 Size: 53 83 186 936 2683 382 635 1451 Fragments arranged by size: 2683 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2786 4842 5240 5514 5661 6072 2786 Size: 2056 398 274 147 411 3123 Fragments arranged by size: 3123 2056 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3624 3654 1178 Size: 30 165 30 2221 30 3933 Fragments arranged by size: 3933 2221 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6409 BseYI C'CCAG_C Cuts at: 2099 5612 6108 2099 Size: 3513 496 2400 Fragments arranged by size: 3513 2400 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2988 1163 Size: 573 1252 4584 Fragments arranged by size: 4584 1252 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2107 3859 4620 5648 1540 Size: 275 186 106 1752 761 1028 2301 Fragments arranged by size: 2301 1752 1028 761 275 186 106 BsiHKAI G_wGCw'C Cuts at: 4470 4555 5626 4470 Size: 85 1071 5253 Fragments arranged by size: 5253 1071 85 BsiWI C'GTAC_G Cuts at: 2901 2901 Size: 6409 BsmI GAATG_Cn' Cuts at: 3118 3320 3920 4013 3118 Size: 202 600 93 5514 Fragments arranged by size: 5514 600 202 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3299 3728 4470 4555 5626 1391 Size: 399 1509 429 742 85 1071 2174 Fragments arranged by size: 2174 1509 1071 742 429 399 85 BspEI T'CCGG_A Cuts at: 6072 6072 Size: 6409 BspHI T'CATG_A Cuts at: 2958 3430 4298 6028 2958 Size: 472 868 1730 3339 Fragments arranged by size: 3339 1730 868 472 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3764 844 Size: 2920 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4296 1394 Size: 429 2473 3507 Fragments arranged by size: 3507 2473 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4904 5078 65 Size: 1183 3656 174 1396 Fragments arranged by size: 3656 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2061 2786 5053 5240 1700 Size: 110 251 725 2267 187 2869 Fragments arranged by size: 2869 2267 725 251 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6409 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6409 BssSI C'ACGA_G Cuts at: 4469 5481 4469 Size: 1012 5397 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6409 BstBI TT'CG_AA Cuts at: 2455 5213 2455 Size: 2758 3651 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6409 BstXI CCAn_nnnn'nTGG Cuts at: 3235 3451 3235 Size: 216 6193 BstYI r'GATC_y Cuts at: 1972 2887 3448 3739 4106 4491 4508 5960 6046 Size: 915 561 291 367 385 17 1452 86 Cuts at: 6046 6058 6404 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 915 561 385 367 346 291 86 17 12 BstZ17I GTA'TAC Cuts at: 2506 2506 Size: 6409 BtgI C'CryG_G Cuts at: 513 1065 2028 2986 513 Size: 552 963 958 3936 Fragments arranged by size: 3936 963 958 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2089 487 Size: 683 56 863 4807 Fragments arranged by size: 4807 863 683 56 BtsI GCAGTG_nn' Cuts at: 3925 4719 4739 3925 Size: 794 20 5595 Fragments arranged by size: 5595 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6409 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6374 DraI TTT'AAA Cuts at: 2764 3713 2764 Size: 949 5460 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2472 5416 809 Size: 507 1156 2944 1802 Fragments arranged by size: 2944 1802 1156 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2019 3856 4745 Size: 54 1425 50 275 18 189 1837 889 Cuts at: 4745 6374 8 Size: 1629 43 Fragments arranged by size: 1837 1629 1425 889 275 189 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3856 1537 Size: 275 2044 4090 Fragments arranged by size: 4090 2044 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4339 1353 Size: 2986 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4996 5370 5516 387 Size: 1064 138 3407 374 146 1280 Fragments arranged by size: 3407 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2421 2577 2665 3090 3422 3597 3657 Size: 393 789 156 88 425 332 175 60 Cuts at: 3657 3699 4472 5069 5856 6124 1239 Size: 42 773 597 787 268 1524 Fragments arranged by size: 1524 789 787 773 597 425 393 332 268 175 156 88 60 42 EcoRI G'AATT_C Cuts at: 3718 4096 3718 Size: 378 6031 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2327 2366 2327 Size: 39 6370 FspI TGC'GCA Cuts at: 4915 4915 Size: 6409 HaeII r_GCGC'y Cuts at: 1678 5556 1678 Size: 3878 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2354 2386 2477 2509 5050 Size: 32 135 32 782 32 91 32 2541 Cuts at: 5050 5082 5124 5156 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2541 782 135 91 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3753 3999 669 Size: 988 281 1815 246 3079 Fragments arranged by size: 3079 1815 988 281 246 HindIII A'AGCT_T Cuts at: 748 2140 748 Size: 1392 5017 HpaI GTT'AAC Cuts at: 3999 3999 Size: 6409 KpnI G_GTAC'C Cuts at: 2184 2986 3734 2184 Size: 802 748 4859 Fragments arranged by size: 4859 802 748 MfeI C'AATT_G Cuts at: 4008 4008 Size: 6409 MluI A'CGCG_T Cuts at: 6285 6285 Size: 6409 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5523 5707 1305 Size: 4218 184 2007 Fragments arranged by size: 4218 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4587 Fragments arranged by size: 4587 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3233 4367 4726 4885 518 Size: 648 2067 1134 359 159 2042 Fragments arranged by size: 2067 2042 1134 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2030 3825 4502 5650 5895 1606 Size: 424 1795 677 1148 245 2120 Fragments arranged by size: 2120 1795 1148 677 424 245 NaeI GCC'GGC Cuts at: 2063 2063 Size: 6409 NcoI C'CATG_G Cuts at: 513 1065 2986 513 Size: 552 1921 3936 Fragments arranged by size: 3936 1921 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6409 NgoMIV G'CCGG_C Cuts at: 2061 2061 Size: 6409 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6409 NotI GC'GGCC_GC Cuts at: 3856 3856 Size: 6409 NspI r_CATG'y Cuts at: 1132 1229 3692 3767 5312 1132 Size: 97 2463 75 1545 2229 Fragments arranged by size: 2463 2229 1545 97 75 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6409 PciI A'CATG_T Cuts at: 3688 5308 3688 Size: 1620 4789 PflMI CCAn_nnn'nTGG Cuts at: 1108 2177 1108 Size: 1069 5340 PfoI T'CCnGG_A Cuts at: 3399 3399 Size: 6409 PmeI GTTT'AAAC Cuts at: 3713 3713 Size: 6409 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4478 4510 5991 6023 4478 Size: 32 1481 32 4864 Fragments arranged by size: 4864 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6409 PsiI TTA'TAA Cuts at: 3218 3979 3218 Size: 761 5648 PstI C_TGCA'G Cuts at: 830 3205 3761 830 Size: 2375 556 3478 Fragments arranged by size: 3478 2375 556 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6409 SacII CC_GC'GG Cuts at: 2031 2031 Size: 6409 SalI G'TCGA_C Cuts at: 1936 3751 1936 Size: 1815 4594 SbfI CC_TGCA'GG Cuts at: 3761 3761 Size: 6409 ScaI AGT'ACT Cuts at: 4657 6369 4657 Size: 1712 4697 SfcI C'TryA_G Cuts at: 826 1045 2161 3201 3757 4892 5573 5764 826 Size: 219 1116 1040 556 1135 681 191 1471 Fragments arranged by size: 1471 1135 1116 1040 681 556 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6409 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6409 SmaI CCC'GGG Cuts at: 1872 3736 1872 Size: 1864 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2312 2351 2509 3650 4513 5414 Size: 197 926 369 39 158 1141 863 901 Cuts at: 5414 5676 820 Size: 262 1553 Fragments arranged by size: 1553 1141 926 901 863 369 262 197 158 39 SnaBI TAC'GTA Cuts at: 493 4200 493 Size: 3707 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6409 SphI G_CATG'C Cuts at: 1132 3767 1132 Size: 2635 3774 SspI AAT'ATT Cuts at: 5 52 2637 3616 4333 5 Size: 47 2585 979 717 2081 Fragments arranged by size: 2585 2081 979 717 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6409 StyI C'CwwG_G Cuts at: 513 1065 2986 3277 3848 513 Size: 552 1921 291 571 3074 Fragments arranged by size: 3074 1921 571 552 291 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2954 3278 4436 4453 4606 4791 5236 Size: 1255 1374 324 1158 17 153 185 445 Cuts at: 5236 325 Size: 1498 Fragments arranged by size: 1498 1374 1255 1158 445 324 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2202 2376 3170 Size: 274 80 33 51 1443 225 174 794 Cuts at: 3170 3379 4655 6367 96 Size: 209 1276 1712 138 Fragments arranged by size: 1712 1443 1276 794 274 225 209 174 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2706 2738 6369 6401 2706 Size: 32 3631 32 2714 Fragments arranged by size: 3631 2714 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2472 1316 Size: 1156 5253 XbaI T'CTAG_A Cuts at: 2443 3745 2443 Size: 1302 5107 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6409 XmaI C'CCGG_G Cuts at: 1870 3734 1870 Size: 1864 4545 XmnI GAAnn'nnTTC Cuts at: 2143 2390 2451 3027 4538 2143 Size: 247 61 576 1511 4014 Fragments arranged by size: 4014 1511 576 247 61 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4217 276 Size: 53 83 186 936 2683 2468 Fragments arranged by size: 2683 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsmBI Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI