(Circular) (Six-base) MAPSORT of: pFN21AB8385.seq Check: 5007 from: 1 to: 5974 pFN21AB8385 5974 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2318 2908 2318 Size: 590 5384 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3784 278 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2946 3317 1937 Size: 1009 371 4594 Fragments arranged by size: 4594 1009 371 Acc65I G'GTAC_C Cuts at: 3295 3295 Size: 5974 AclI AA'CG_TT Cuts at: 1563 4101 4474 1563 Size: 2538 373 3063 Fragments arranged by size: 3063 2538 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2671 4037 5421 5689 1632 Size: 1039 1366 1384 268 1917 Fragments arranged by size: 1917 1384 1366 1039 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5777 AflIII A'CryG_T Cuts at: 1203 2941 3255 4873 5850 1203 Size: 1738 314 1618 977 1327 Fragments arranged by size: 1738 1618 1327 977 314 AgeI A'CCGG_T Cuts at: 4805 4805 Size: 5974 AhdI GACnn_n'nnGTC Cuts at: 2749 4703 2749 Size: 1954 4020 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5974 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3131 1184 Size: 1947 4027 AlwNI CAG_nnn'CTG Cuts at: 2385 3717 5289 2385 Size: 1332 1572 3070 Fragments arranged by size: 3070 1572 1332 ApaI G_GGCC'C Cuts at: 2225 2707 2225 Size: 482 5492 ApaLI G'TGCA_C Cuts at: 4031 5187 4031 Size: 1156 4818 ApoI r'AATT_y Cuts at: 1493 3283 3509 3661 5770 1493 Size: 1790 226 152 2109 1697 Fragments arranged by size: 2109 1790 1697 226 152 AscI GG'CGCG_CC Cuts at: 2145 2145 Size: 5974 AseI AT'TA_AT Cuts at: 160 1032 4528 160 Size: 872 3496 1606 Fragments arranged by size: 3496 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 AvaI C'yCGr_G Cuts at: 1870 1943 2140 2167 3299 1870 Size: 73 197 27 1132 4545 Fragments arranged by size: 4545 1132 197 73 27 BamHI G'GATC_C Cuts at: 3304 3671 3304 Size: 367 5607 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2111 2824 3295 4750 618 Size: 325 217 627 324 713 471 1455 1842 Fragments arranged by size: 1842 1455 713 627 471 325 324 217 BanII G_rGCy'C Cuts at: 1391 2141 2225 2595 2707 3293 1391 Size: 750 84 370 112 586 4072 Fragments arranged by size: 4072 750 586 370 112 84 BbeI G_GCGC'C Cuts at: 2115 2828 2115 Size: 713 5261 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5003 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4165 4199 1903 Size: 34 2228 34 3678 Fragments arranged by size: 3678 2228 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3866 5076 3866 Size: 1210 4764 BclI T'GATC_A Cuts at: 1548 1548 Size: 5974 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3105 4585 136 Size: 107 122 71 631 605 1433 1480 1525 Fragments arranged by size: 1525 1480 1433 631 605 122 107 71 BglII A'GATC_T Cuts at: 5969 5969 Size: 5974 BlpI GC'TnA_GC Cuts at: 3391 3391 Size: 5974 Bme1580I G_kGCm'C Cuts at: 1790 2225 2707 4035 5191 1790 Size: 435 482 1328 1156 2573 Fragments arranged by size: 2573 1328 1156 482 435 BmgBI CAC'GTC Cuts at: 1318 2047 2604 2944 2991 1318 Size: 729 557 340 47 4301 Fragments arranged by size: 4301 729 557 340 47 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2794 3705 4663 Size: 416 53 83 399 24 1377 911 958 Cuts at: 4663 442 Size: 1753 Fragments arranged by size: 1753 1377 958 911 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5974 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2791 3080 4634 1239 Size: 1552 289 1554 2579 Fragments arranged by size: 2579 1554 1552 289 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2417 2915 4099 4964 5262 2417 Size: 498 1184 865 298 3129 Fragments arranged by size: 3129 1184 865 498 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4637 882 Size: 617 3138 2219 Fragments arranged by size: 3138 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2821 3070 3765 493 Size: 711 1617 249 695 2702 Fragments arranged by size: 2702 1617 711 695 249 BsaBI GATnn'nnATC Cuts at: 5968 5968 Size: 5974 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2112 2825 2997 3781 Size: 53 83 186 936 579 713 172 784 Cuts at: 3781 4163 4798 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 784 713 635 579 382 186 172 83 53 BsaWI w'CCGG_w Cuts at: 4407 4805 5079 5226 5637 4407 Size: 398 274 147 411 4744 Fragments arranged by size: 4744 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5749 Fragments arranged by size: 5749 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2353 3077 3088 3139 1186 Size: 1167 724 11 51 4021 Fragments arranged by size: 4021 1167 724 51 11 BseYI C'CCAG_C Cuts at: 2496 3095 5177 5673 2496 Size: 599 2082 496 2797 Fragments arranged by size: 2797 2082 599 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2801 1163 Size: 573 1065 4336 Fragments arranged by size: 4336 1065 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2875 3424 4185 5213 1540 Size: 275 186 874 549 761 1028 2301 Fragments arranged by size: 2301 1028 874 761 549 275 186 BsiHKAI G_wGCw'C Cuts at: 2790 2807 3272 4035 4120 5191 2790 Size: 17 465 763 85 1071 3573 Fragments arranged by size: 3573 1071 763 465 85 17 BsmI GAATG_Cn' Cuts at: 2490 3485 3578 2490 Size: 995 93 4886 Fragments arranged by size: 4886 995 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2141 2225 2595 2707 2790 2807 3272 Size: 399 351 84 370 112 83 17 465 Cuts at: 3272 3293 4035 4120 5191 1391 Size: 21 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 465 399 370 351 112 85 84 83 21 17 BspEI T'CCGG_A Cuts at: 5637 5637 Size: 5974 BspHI T'CATG_A Cuts at: 3863 5593 3863 Size: 1730 4244 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2318 2908 3329 844 Size: 1474 590 421 3489 Fragments arranged by size: 3489 1474 590 421 BsrBI CCG'CTC Cuts at: 1394 1823 2286 3861 1394 Size: 429 463 1575 3507 Fragments arranged by size: 3507 1575 463 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2016 4469 4643 65 Size: 1183 768 2453 174 1396 Fragments arranged by size: 2453 1396 1183 768 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2114 2843 4618 4805 1700 Size: 110 304 729 1775 187 2869 Fragments arranged by size: 2869 1775 729 304 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5974 BssHII G'CGCG_C Cuts at: 1925 2145 2584 2675 1925 Size: 220 439 91 5224 Fragments arranged by size: 5224 439 220 91 BssSI C'ACGA_G Cuts at: 4034 5046 4034 Size: 1012 4962 BstAPI GCAn_nnn'nTGC Cuts at: 794 2415 2622 794 Size: 1621 207 4146 Fragments arranged by size: 4146 1621 207 BstBI TT'CG_AA Cuts at: 4778 4778 Size: 5974 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5974 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5974 BstYI r'GATC_y Cuts at: 1972 2032 2728 3304 3671 4056 4073 5525 5611 Size: 60 696 576 367 385 17 1452 86 Cuts at: 5611 5623 5969 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 696 576 385 367 346 86 60 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2095 513 Size: 552 939 91 4392 Fragments arranged by size: 4392 939 552 91 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5235 Fragments arranged by size: 5235 683 56 BtsI GCAGTG_nn' Cuts at: 2548 3056 3490 4284 4304 2548 Size: 508 434 794 20 4218 Fragments arranged by size: 4218 794 508 434 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5974 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5939 DraI TTT'AAA Cuts at: 3278 3278 Size: 5974 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4981 809 Size: 507 3665 1802 Fragments arranged by size: 3665 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2049 2299 2545 Size: 54 1425 50 275 18 219 250 246 Cuts at: 2545 2670 2835 3421 4310 5939 8 Size: 125 165 586 889 1629 43 Fragments arranged by size: 1629 1425 889 586 275 250 246 219 165 125 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3421 1537 Size: 275 1609 4090 Fragments arranged by size: 4090 1609 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2690 3173 3904 1353 Size: 1337 483 731 3423 Fragments arranged by size: 3423 1337 731 483 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4561 4935 5081 387 Size: 1064 138 2972 374 146 1280 Fragments arranged by size: 2972 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2671 2791 3080 4037 4634 5421 5689 Size: 393 1039 120 289 957 597 787 268 Cuts at: 5689 1239 Size: 1524 Fragments arranged by size: 1524 1039 957 787 597 393 289 268 120 EcoO109I rG'GnC_Cy Cuts at: 2221 2533 2221 Size: 312 5662 EcoRI G'AATT_C Cuts at: 3283 3661 3283 Size: 378 5596 FspI TGC'GCA Cuts at: 3055 3137 4480 3055 Size: 82 1343 4549 Fragments arranged by size: 4549 1343 82 FspAI rTGC'GCAy Cuts at: 3055 3055 Size: 5974 HaeII r_GCGC'y Cuts at: 1678 2115 2186 2694 2828 2939 5121 1678 Size: 437 71 508 134 111 2182 2531 Fragments arranged by size: 2531 2182 508 437 134 111 71 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2735 2767 4615 4647 4689 Size: 32 135 32 1163 32 1848 32 42 Cuts at: 4689 4721 1373 Size: 32 2626 Fragments arranged by size: 2626 1848 1163 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2947 3318 3564 669 Size: 988 281 1009 371 246 3079 Fragments arranged by size: 3079 1009 988 371 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5974 HpaI GTT'AAC Cuts at: 3564 3564 Size: 5974 KasI G'GCGC_C Cuts at: 2111 2824 2111 Size: 713 5261 KpnI G_GTAC'C Cuts at: 3299 3299 Size: 5974 MfeI C'AATT_G Cuts at: 3573 3573 Size: 5974 MluI A'CGCG_T Cuts at: 5850 5850 Size: 5974 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2516 2991 5088 5272 1305 Size: 1211 475 2097 184 2007 Fragments arranged by size: 2097 2007 1211 475 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4152 Fragments arranged by size: 4152 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3932 4291 4450 518 Size: 648 843 1923 359 159 2042 Fragments arranged by size: 2042 1923 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2025 2097 2815 2964 3390 4067 5215 5460 Size: 419 72 718 149 426 677 1148 245 Cuts at: 5460 1606 Size: 2120 Fragments arranged by size: 2120 1148 718 677 426 419 245 149 72 NaeI GCC'GGC Cuts at: 2116 2845 2116 Size: 729 5245 NarI GG'CG_CC Cuts at: 2112 2825 2112 Size: 713 5261 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4483 Fragments arranged by size: 4483 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5974 NgoMIV G'CCGG_C Cuts at: 2114 2843 2114 Size: 729 5245 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5974 NotI GC'GGCC_GC Cuts at: 3421 3421 Size: 5974 NspI r_CATG'y Cuts at: 1132 1229 2370 2629 2855 3259 3332 4877 1132 Size: 97 1141 259 226 404 73 1545 2229 Fragments arranged by size: 2229 1545 1141 404 259 226 97 73 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5974 PciI A'CATG_T Cuts at: 3255 4873 3255 Size: 1618 4356 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5974 PfoI T'CCnGG_A Cuts at: 2724 2910 2724 Size: 186 5788 PmeI GTTT'AAAC Cuts at: 3278 3278 Size: 5974 PmlI CAC'GTG Cuts at: 2821 2821 Size: 5974 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4043 4075 5556 5588 4043 Size: 32 1481 32 4429 Fragments arranged by size: 4429 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2533 2533 Size: 5974 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5974 PsiI TTA'TAA Cuts at: 3544 3544 Size: 5974 PspOMI G'GGCC_C Cuts at: 2221 2703 2221 Size: 482 5492 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2345 2377 2345 Size: 32 5942 PstI C_TGCA'G Cuts at: 830 2736 3039 3326 830 Size: 1906 303 287 3478 Fragments arranged by size: 3478 1906 303 287 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5974 PvuII CAG'CTG Cuts at: 2025 2964 2025 Size: 939 5035 SacII CC_GC'GG Cuts at: 2098 2098 Size: 5974 SalI G'TCGA_C Cuts at: 1936 2945 3316 1936 Size: 1009 371 4594 Fragments arranged by size: 4594 1009 371 SanDI GG'GwC_CC Cuts at: 2533 2533 Size: 5974 SapI GCTCTTCn'nnn_ Cuts at: 2690 3173 2690 Size: 483 5491 SbfI CC_TGCA'GG Cuts at: 3039 3326 3039 Size: 287 5687 ScaI AGT'ACT Cuts at: 4222 5934 4222 Size: 1712 4262 SexAI A'CCwGG_T Cuts at: 2745 2745 Size: 5974 SfcI C'TryA_G Cuts at: 826 1045 2732 3035 3322 4457 5138 5329 826 Size: 219 1687 303 287 1135 681 191 1471 Fragments arranged by size: 1687 1471 1135 681 303 287 219 191 SfoI GGC'GCC Cuts at: 2113 2826 2113 Size: 713 5261 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 SgrAI Cr'CCGG_yG Cuts at: 1700 2114 1700 Size: 414 5560 SmaI CCC'GGG Cuts at: 1872 2169 3301 1872 Size: 297 1132 4545 Fragments arranged by size: 4545 1132 297 SmlI C'TyrA_G Cuts at: 820 1017 1943 2432 2930 4078 4979 5241 820 Size: 197 926 489 498 1148 901 262 1553 Fragments arranged by size: 1553 1148 926 901 498 489 262 197 SnaBI TAC'GTA Cuts at: 493 3765 493 Size: 3272 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5974 SphI G_CATG'C Cuts at: 1132 2629 2855 3332 1132 Size: 1497 226 477 3774 Fragments arranged by size: 3774 1497 477 226 SspI AAT'ATT Cuts at: 5 52 3898 5 Size: 47 3846 2081 Fragments arranged by size: 3846 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5974 StyI C'CwwG_G Cuts at: 513 1065 2004 3413 513 Size: 552 939 1409 3074 Fragments arranged by size: 3074 1409 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4001 4018 4171 4356 4801 325 Size: 1255 2421 17 153 185 445 1498 Fragments arranged by size: 2421 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2958 4220 5932 Size: 274 80 33 51 1443 981 1262 1712 Cuts at: 5932 96 Size: 138 Fragments arranged by size: 1712 1443 1262 981 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5934 5966 5934 Size: 32 5942 Tth111I GACn'n_nGTC Cuts at: 1316 2876 1316 Size: 1560 4414 XbaI T'CTAG_A Cuts at: 3310 3310 Size: 5974 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2641 2641 Size: 5974 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5974 XmaI C'CCGG_G Cuts at: 1870 2167 3299 1870 Size: 297 1132 4545 Fragments arranged by size: 4545 1132 297 XmnI GAAnn'nnTTC Cuts at: 2469 4103 2469 Size: 1634 4340 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3782 276 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI NruI NsiI PacI PspXI RsrII SacI SfiI SrfI SwaI