Restriction map for
pFN21AB8383
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BcnI BfaI BfrI
BglI BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsmFI BsoBI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DraII DraIII DrdI DsaI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT14I
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MaeI MamI MfeI MluI
MroI MspA1I MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I StuI StyI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AciI 824 GCGG CCGC(-3/-1)
AflIII 260 ACGTGT A^C[GA][CT]GT
AhdI 1665 GACCTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 380 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 595 ATTAAT AT^TAAT
AsnI 595 ATTAAT AT^TAAT
AspEI 1665 GACCTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 75 GGACC G^G[AT]CC
BalI 630 TGGCCA TGG^CCA
BbsI 1662 GAAGAC GAAGAC(2/6)
BclI 576 TGATCA T^GATCA
BglII 700 AGATCT A^GATCT
BpuAI 1662 GAAGAC GAAGAC(2/6)
BsmI 588 GAATGC GAATGC(1/-1)
Bsp1407I 1524 TGTACA T^GTACA
BsrDI 1317 GCAATG GCAATG(2/0)
BsrGI 1524 TGTACA T^GTACA
BstAPI 815 GCAGTATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr13I 75 GGACC G^G[ACGT]CC
DraI 762 TTTAAA TTT^AAA
EaeI 630 TGGCCA [CT]^GGCC[GA]
Eam1105I 1665 GACCTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1703 GAATTC G^AATTC
FauI 824 GCGGG CCCGC(4/6)
FbaI 576 TGATCA T^GATCA
HaeIII 631 GGCC GG^CC
MaeII 260 ACGT A^CGT
MluNI 630 TGGCCA TGG^CCA
MscI 630 TGGCCA TGG^CCA
MwoI 815 GCAGTATTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflMI 622 CCAAGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 595 ATTAAT AT^TAAT
SapI 190 GAAGAGC GCTCTTC(1/4)
Sau96I 75 GGACC G^G[ACGT]CC
SspBI 1524 TGTACA T^GTACA
SspI 445 AATATT AAT^ATT
TaqI 179 TCGA T^CGA
TthHB8I 179 TCGA T^CGA
Van91I 622 CCAAGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 235 CCAGAAAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 108 GATCC GGATC(4/5)
609 GGATC
BbvI 272 GCAGC GCAGC(8/12)
679 GCAGC
BsmAI 715 GAGAC GTCTC(1/5)
133 GTCTC
BsrI 1543 ACTGG ACTGG(1/-1)
225 CCAGT
BstYI 609 GGATCT [GA]^GATC[CT]
700 AGATCT
EarI 58 GAAGAG CTCTTC(1/4)
190 GAAGAG
EcoT22I 1400 ATGCAT ATGCA^T
1652 ATGCAT
Fnu4HI 272 GCAGC GC^[ACGT]GC
679 GCAGC
ItaI 272 GCAGC GC^[ACGT]GC
679 GCAGC
Ksp632I 58 GAAGAG CTCTTC(1/4)
190 GAAGAG
MflI 609 GGATCT [GA]^GATC[CT]
700 AGATCT
MslI 1556 CATATAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1710 CACAAGGGTG
NlaIV 75 GGACCC GG[ACGT]^[ACGT]CC
801 GGAGCC
NsiI 1400 ATGCAT ATGCA^T
1652 ATGCAT
NspI 1402 GCATGT [GA]CATG^[CT]
1654 GCATGT
PleI 421 GACTC GAGTC(4/5)
1620 GACTC
SacI 866 GAGCTC GAGCT^C
1115 GAGCTC
SfaNI 812 GATGC GCATC(5/9)
1443 GCATC
TseI 272 GCAGC G^C[AT]GC
679 GCAGC
XhoII 609 GGATCT [GA]^GATC[CT]
700 AGATCT
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AcsI AfaI AluI ApoI Asp700I
AspHI BanII BmyI BsaJI BsiHKAI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI DdeI DpnII EcoRII FokI
HinfI HphI MaeIII MboI MboII
MnlI MseI MvaI NdeII NlaIII
RsaI Sau3AI ScrFI TfiI Tru9I
Tsp45I Tsp509I TspRI XmnI