(Circular) (Six-base) MAPSORT of: pFN21AB8383.seq Check: 6802 from: 1 to: 6442 pFN21AB8383 6442 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4252 278 Size: 53 83 186 936 2716 2468 Fragments arranged by size: 2716 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3785 1937 Size: 1848 4594 Acc65I G'GTAC_C Cuts at: 3763 3763 Size: 6442 AclI AA'CG_TT Cuts at: 1563 4569 4942 1563 Size: 3006 373 3063 Fragments arranged by size: 3063 3006 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4505 5889 6157 1632 Size: 2873 1384 268 1917 Fragments arranged by size: 2873 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6245 AflIII A'CryG_T Cuts at: 1203 2265 5341 6318 1203 Size: 1062 3076 977 1327 Fragments arranged by size: 3076 1327 1062 977 AgeI A'CCGG_T Cuts at: 5273 5273 Size: 6442 AhdI GACnn_n'nnGTC Cuts at: 3675 5171 3675 Size: 1496 4946 AleI CACnn'nnGTG Cuts at: 1166 3719 1166 Size: 2553 3889 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6442 AlwNI CAG_nnn'CTG Cuts at: 2390 4185 5757 2390 Size: 1795 1572 3075 Fragments arranged by size: 3075 1795 1572 ApaLI G'TGCA_C Cuts at: 4499 5655 4499 Size: 1156 5286 ApoI r'AATT_y Cuts at: 1493 2440 2848 3708 3751 3977 4129 6238 1493 Size: 947 408 860 43 226 152 2109 1697 Fragments arranged by size: 2109 1697 947 860 408 226 152 43 AseI AT'TA_AT Cuts at: 160 1032 2601 4996 160 Size: 872 1569 2395 1606 Fragments arranged by size: 2395 1606 1569 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6442 AvaI C'yCGr_G Cuts at: 1870 1943 3767 1870 Size: 73 1824 4545 Fragments arranged by size: 4545 1824 73 BamHI G'GATC_C Cuts at: 3772 4139 3772 Size: 367 6075 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3763 5218 618 Size: 325 217 627 1976 1455 1842 Fragments arranged by size: 1976 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2202 2875 3124 3761 1391 Size: 811 673 249 637 4072 Fragments arranged by size: 4072 811 673 637 249 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3674 928 Size: 971 1775 3696 Fragments arranged by size: 3696 1775 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4633 4667 1903 Size: 34 2696 34 3678 Fragments arranged by size: 3678 2696 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4334 5544 4334 Size: 1210 5232 BclI T'GATC_A Cuts at: 1548 2581 1548 Size: 1033 5409 BfrBI ATG'CAT Cuts at: 3407 3659 3407 Size: 252 6190 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5053 136 Size: 107 122 71 631 605 3381 1525 Fragments arranged by size: 3381 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2705 6437 2705 Size: 3732 2710 BlpI GC'TnA_GC Cuts at: 3859 3859 Size: 6442 Bme1580I G_kGCm'C Cuts at: 1790 2384 4503 5659 1790 Size: 594 2119 1156 2573 Fragments arranged by size: 2573 2119 1156 594 BmgBI CAC'GTC Cuts at: 1318 2266 1318 Size: 948 5494 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3558 4173 5131 Size: 416 53 83 399 24 2141 615 958 Cuts at: 5131 442 Size: 1753 Fragments arranged by size: 2141 1753 958 615 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6442 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5102 1239 Size: 3863 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4567 5432 5730 4567 Size: 865 298 5279 Fragments arranged by size: 5279 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5105 882 Size: 617 3606 2219 Fragments arranged by size: 3606 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4233 493 Size: 711 3029 2702 Fragments arranged by size: 3029 2702 711 BsaBI GATnn'nnATC Cuts at: 6436 6436 Size: 6442 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4249 4631 5266 275 Size: 53 83 186 936 2716 382 635 1451 Fragments arranged by size: 2716 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4875 5273 5547 5694 6105 4875 Size: 398 274 147 411 5212 Fragments arranged by size: 5212 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2709 2739 3461 3491 3629 Size: 30 165 30 1306 30 722 30 138 Cuts at: 3629 3659 1178 Size: 30 3961 Fragments arranged by size: 3961 1306 722 165 138 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6442 BseYI C'CCAG_C Cuts at: 3108 5645 6141 3108 Size: 2537 496 3409 Fragments arranged by size: 3409 2537 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5869 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3892 4653 5681 1540 Size: 275 186 1891 761 1028 2301 Fragments arranged by size: 2301 1891 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2875 3124 3693 4503 4588 5659 2875 Size: 249 569 810 85 1071 3658 Fragments arranged by size: 3658 1071 810 569 249 85 BsmI GAATG_Cn' Cuts at: 2599 3953 4046 2599 Size: 1354 93 4995 Fragments arranged by size: 4995 1354 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2202 2384 2875 3124 3693 3761 4503 Size: 399 412 182 491 249 569 68 742 Cuts at: 4503 4588 5659 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 569 491 412 399 249 182 85 68 BspEI T'CCGG_A Cuts at: 6105 6105 Size: 6442 BspHI T'CATG_A Cuts at: 4331 6061 4331 Size: 1730 4712 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3797 844 Size: 2953 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4329 1394 Size: 429 2506 3507 Fragments arranged by size: 3507 2506 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3329 4937 5111 65 Size: 1183 2081 1608 174 1396 Fragments arranged by size: 2081 1608 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5086 5273 1700 Size: 110 3276 187 2869 Fragments arranged by size: 3276 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3529 96 Size: 3433 3009 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6442 BssSI C'ACGA_G Cuts at: 4502 5514 4502 Size: 1012 5430 BstAPI GCAn_nnn'nTGC Cuts at: 794 2826 794 Size: 2032 4410 BstBI TT'CG_AA Cuts at: 5246 5246 Size: 6442 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6442 BstYI r'GATC_y Cuts at: 1972 2614 2705 3772 4139 4524 4541 5993 6079 Size: 642 91 1067 367 385 17 1452 86 Cuts at: 6079 6091 6437 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1067 642 385 367 346 91 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5890 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5703 Fragments arranged by size: 5703 683 56 BtsI GCAGTG_nn' Cuts at: 3958 4752 4772 3958 Size: 794 20 5628 Fragments arranged by size: 5628 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6442 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6407 DraI TTT'AAA Cuts at: 2769 3746 2769 Size: 977 5465 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5449 809 Size: 507 4133 1802 Fragments arranged by size: 4133 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2635 3889 4778 Size: 54 1425 50 275 18 805 1254 889 Cuts at: 4778 6407 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1254 889 805 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3889 1537 Size: 275 2077 4090 Fragments arranged by size: 4090 2077 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2058 2190 4372 1353 Size: 705 132 2182 3423 Fragments arranged by size: 3423 2182 705 132 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5029 5403 5549 387 Size: 1064 138 3440 374 146 1280 Fragments arranged by size: 3440 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2873 3122 2873 Size: 249 6193 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4505 5102 5889 6157 1239 Size: 393 2873 597 787 268 1524 Fragments arranged by size: 2873 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 3708 3751 4129 3708 Size: 43 378 6021 Fragments arranged by size: 6021 378 43 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2385 2385 Size: 6442 FspI TGC'GCA Cuts at: 4948 4948 Size: 6442 HaeII r_GCGC'y Cuts at: 1678 5589 1678 Size: 3911 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2097 2129 2709 2741 3265 Size: 32 135 32 525 32 580 32 524 Cuts at: 3265 3297 3423 3455 3661 3693 5083 5115 5157 Size: 32 126 32 206 32 1390 32 42 Cuts at: 5157 5189 1373 Size: 32 2626 Fragments arranged by size: 2626 1390 580 525 524 206 135 126 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3786 4032 669 Size: 988 281 1848 246 3079 Fragments arranged by size: 3079 1848 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6442 HpaI GTT'AAC Cuts at: 4032 4032 Size: 6442 KpnI G_GTAC'C Cuts at: 3767 3767 Size: 6442 MfeI C'AATT_G Cuts at: 4041 4041 Size: 6442 MluI A'CGCG_T Cuts at: 6318 6318 Size: 6442 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5556 5740 1305 Size: 4251 184 2007 Fragments arranged by size: 4251 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2637 10 Size: 54 1425 343 805 3815 Fragments arranged by size: 3815 1425 805 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3565 3719 4400 4759 4918 518 Size: 648 2399 154 681 359 159 2042 Fragments arranged by size: 2399 2042 681 648 359 159 154 MspA1I CmG'CkG Cuts at: 1606 3858 4535 5683 5928 1606 Size: 2252 677 1148 245 2120 Fragments arranged by size: 2252 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5890 NdeI CA'TA_TG Cuts at: 387 387 Size: 6442 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6442 NotI GC'GGCC_GC Cuts at: 3889 3889 Size: 6442 NsiI A_TGCA'T Cuts at: 3409 3661 3409 Size: 252 6190 NspI r_CATG'y Cuts at: 1132 1229 3411 3663 3800 5345 1132 Size: 97 2182 252 137 1545 2229 Fragments arranged by size: 2229 2182 1545 252 137 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6442 PciI A'CATG_T Cuts at: 5341 5341 Size: 6442 PflMI CCAn_nnn'nTGG Cuts at: 1108 2633 1108 Size: 1525 4917 PfoI T'CCnGG_A Cuts at: 2115 2115 Size: 6442 PmeI GTTT'AAAC Cuts at: 3746 3746 Size: 6442 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4511 4543 6024 6056 4511 Size: 32 1481 32 4897 Fragments arranged by size: 4897 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6442 PsiI TTA'TAA Cuts at: 2574 4012 2574 Size: 1438 5004 PstI C_TGCA'G Cuts at: 830 3794 830 Size: 2964 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6442 SacI G_AGCT'C Cuts at: 2875 3124 2875 Size: 249 6193 SalI G'TCGA_C Cuts at: 1936 3784 1936 Size: 1848 4594 SapI GCTCTTCn'nnn_ Cuts at: 2190 2190 Size: 6442 SbfI CC_TGCA'GG Cuts at: 3794 3794 Size: 6442 ScaI AGT'ACT Cuts at: 4690 6402 4690 Size: 1712 4730 SfcI C'TryA_G Cuts at: 826 1045 3790 4925 5606 5797 826 Size: 219 2745 1135 681 191 1471 Fragments arranged by size: 2745 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6442 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6442 SmaI CCC'GGG Cuts at: 1872 3769 1872 Size: 1897 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4546 5447 5709 820 Size: 197 926 2603 901 262 1553 Fragments arranged by size: 2603 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4233 493 Size: 3740 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6442 SphI G_CATG'C Cuts at: 1132 3800 1132 Size: 2668 3774 SspI AAT'ATT Cuts at: 5 52 2452 4366 5 Size: 47 2400 1914 2081 Fragments arranged by size: 2400 2081 1914 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6442 StyI C'CwwG_G Cuts at: 513 1065 3881 513 Size: 552 2816 3074 Fragments arranged by size: 3074 2816 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2253 4469 4486 4639 4824 5269 325 Size: 1255 673 2216 17 153 185 445 1498 Fragments arranged by size: 2216 1498 1255 673 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3037 3233 3529 Size: 274 80 33 51 1443 1060 196 296 Cuts at: 3529 4688 6400 96 Size: 1159 1712 138 Fragments arranged by size: 1712 1443 1159 1060 296 274 196 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6402 6434 6402 Size: 32 6410 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6442 XbaI T'CTAG_A Cuts at: 3778 3778 Size: 6442 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2247 2247 Size: 6442 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6442 XmaI C'CCGG_G Cuts at: 1870 3767 1870 Size: 1897 4545 XmnI GAAnn'nnTTC Cuts at: 2837 3167 3419 3587 4571 2837 Size: 330 252 168 984 4708 Fragments arranged by size: 4708 984 330 252 168 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4250 276 Size: 53 83 186 936 2716 2468 Fragments arranged by size: 2716 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI