(Circular) (Six-base) MAPSORT of: pFN21AB8381.seq Check: 7624 from: 1 to: 6211 pFN21AB8381 6211 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4021 278 Size: 53 83 186 936 2485 2468 Fragments arranged by size: 2485 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3554 1937 Size: 1617 4594 Acc65I G'GTAC_C Cuts at: 3532 3532 Size: 6211 AclI AA'CG_TT Cuts at: 1563 4338 4711 1563 Size: 2775 373 3063 Fragments arranged by size: 3063 2775 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2039 2375 3527 4274 5658 5926 1632 Size: 407 336 1152 747 1384 268 1917 Fragments arranged by size: 1917 1384 1152 747 407 336 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6014 AflIII A'CryG_T Cuts at: 1203 5110 6087 1203 Size: 3907 977 1327 Fragments arranged by size: 3907 1327 977 AgeI A'CCGG_T Cuts at: 5042 5042 Size: 6211 AhdI GACnn_n'nnGTC Cuts at: 4940 4940 Size: 6211 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6211 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6211 AlwNI CAG_nnn'CTG Cuts at: 2101 3954 5526 2101 Size: 1853 1572 2786 Fragments arranged by size: 2786 1853 1572 ApaLI G'TGCA_C Cuts at: 2570 4268 5424 2570 Size: 1698 1156 3357 Fragments arranged by size: 3357 1698 1156 ApoI r'AATT_y Cuts at: 1493 2292 2448 3399 3520 3746 3898 6007 1493 Size: 799 156 951 121 226 152 2109 1697 Fragments arranged by size: 2109 1697 951 799 226 156 152 121 AseI AT'TA_AT Cuts at: 160 1032 2337 4765 160 Size: 872 1305 2428 1606 Fragments arranged by size: 2428 1606 1305 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6211 AvaI C'yCGr_G Cuts at: 1870 1943 2846 3536 1870 Size: 73 903 690 4545 Fragments arranged by size: 4545 903 690 73 AvrII C'CTAG_G Cuts at: 2492 2492 Size: 6211 BamHI G'GATC_C Cuts at: 3541 3908 3541 Size: 367 5844 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2111 2623 3532 4987 618 Size: 325 217 627 324 512 909 1455 1842 Fragments arranged by size: 1842 1455 909 627 512 325 324 217 BanII G_rGCy'C Cuts at: 1391 2017 3530 1391 Size: 626 1513 4072 Fragments arranged by size: 4072 1513 626 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5240 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3005 3039 4402 4436 1903 Size: 34 1068 34 1363 34 3678 Fragments arranged by size: 3678 1363 1068 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4103 5313 4103 Size: 1210 5001 BclI T'GATC_A Cuts at: 1548 1548 Size: 6211 BfrBI ATG'CAT Cuts at: 3143 3143 Size: 6211 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4822 136 Size: 107 122 71 631 605 3150 1525 Fragments arranged by size: 3150 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6206 6206 Size: 6211 BlpI GC'TnA_GC Cuts at: 3003 3339 3628 3003 Size: 336 289 5586 Fragments arranged by size: 5586 336 289 Bme1580I G_kGCm'C Cuts at: 1790 2574 4272 5428 1790 Size: 784 1698 1156 2573 Fragments arranged by size: 2573 1698 1156 784 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6211 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2111 3942 4900 Size: 416 53 83 399 24 694 1831 958 Cuts at: 4900 442 Size: 1753 Fragments arranged by size: 1831 1753 958 694 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2079 1056 Size: 1023 5188 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2190 2190 Size: 6211 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2217 2443 2460 2943 4871 1239 Size: 978 226 17 483 1928 2579 Fragments arranged by size: 2579 1928 978 483 226 17 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4336 5201 5499 4336 Size: 865 298 5048 Fragments arranged by size: 5048 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4874 882 Size: 617 3375 2219 Fragments arranged by size: 3375 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2856 4002 493 Size: 711 1652 1146 2702 Fragments arranged by size: 2702 1652 1146 711 BsaBI GATnn'nnATC Cuts at: 3104 3492 6205 3104 Size: 388 2713 3110 Fragments arranged by size: 3110 2713 388 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4018 4400 5035 275 Size: 53 83 186 936 2485 382 635 1451 Fragments arranged by size: 2485 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4644 5042 5316 5463 5874 4644 Size: 398 274 147 411 4981 Fragments arranged by size: 4981 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2516 2546 1178 Size: 30 165 30 1113 30 4843 Fragments arranged by size: 4843 1113 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6211 BseYI C'CCAG_C Cuts at: 5414 5910 5414 Size: 496 5715 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2081 2751 1163 Size: 573 345 670 4623 Fragments arranged by size: 4623 670 573 345 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3661 4422 5450 1540 Size: 275 186 1660 761 1028 2301 Fragments arranged by size: 2301 1660 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2574 2685 3411 4272 4357 5428 2574 Size: 111 726 861 85 1071 3357 Fragments arranged by size: 3357 1071 861 726 111 85 BsmI GAATG_Cn' Cuts at: 2304 3722 3815 2304 Size: 1418 93 4700 Fragments arranged by size: 4700 1418 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2017 2574 2685 3411 3530 4272 4357 Size: 399 227 557 111 726 119 742 85 Cuts at: 4357 5428 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 726 557 399 227 119 111 85 BspEI T'CCGG_A Cuts at: 5874 5874 Size: 6211 BspHI T'CATG_A Cuts at: 2782 4100 5830 2782 Size: 1318 1730 3163 Fragments arranged by size: 3163 1730 1318 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3566 844 Size: 2722 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4098 1394 Size: 429 2275 3507 Fragments arranged by size: 3507 2275 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2138 4706 4880 65 Size: 1183 890 2568 174 1396 Fragments arranged by size: 2568 1396 1183 890 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4855 5042 1700 Size: 110 3045 187 2869 Fragments arranged by size: 3045 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6211 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6211 BssSI C'ACGA_G Cuts at: 4271 5283 4271 Size: 1012 5199 BstAPI GCAn_nnn'nTGC Cuts at: 794 3013 794 Size: 2219 3992 BstBI TT'CG_AA Cuts at: 2296 5015 2296 Size: 2719 3492 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6211 BstXI CCAn_nnnn'nTGG Cuts at: 3110 3110 Size: 6211 BstYI r'GATC_y Cuts at: 1972 3541 3908 4293 4310 5762 5848 5860 6206 Size: 1569 367 385 17 1452 86 12 346 Cuts at: 6206 1972 Size: 1977 Fragments arranged by size: 1977 1569 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2994 2994 Size: 6211 BtgI C'CryG_G Cuts at: 513 1065 2004 2840 513 Size: 552 939 836 3884 Fragments arranged by size: 3884 939 836 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5472 Fragments arranged by size: 5472 683 56 BtsI GCAGTG_nn' Cuts at: 2244 3014 3197 3727 4521 4541 2244 Size: 770 183 530 794 20 3914 Fragments arranged by size: 3914 794 770 530 183 20 ClaI AT'CG_AT Cuts at: 1554 2487 1554 Size: 933 5278 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6176 DraI TTT'AAA Cuts at: 3515 3515 Size: 6211 DraIII CAC_nnn'GTG Cuts at: 2832 2832 Size: 6211 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5218 809 Size: 507 3902 1802 Fragments arranged by size: 3902 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2031 2092 2694 Size: 54 1425 50 275 18 201 61 602 Cuts at: 2694 3658 4547 6176 8 Size: 964 889 1629 43 Fragments arranged by size: 1629 1425 964 889 602 275 201 61 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3658 1537 Size: 275 1846 4090 Fragments arranged by size: 4090 1846 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2154 2348 4141 1353 Size: 801 194 1793 3423 Fragments arranged by size: 3423 1793 801 194 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4798 5172 5318 387 Size: 1064 138 3209 374 146 1280 Fragments arranged by size: 3209 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2039 2217 2375 2443 2460 2943 3527 Size: 393 407 178 158 68 17 483 584 Cuts at: 3527 4274 4871 5658 5926 1239 Size: 747 597 787 268 1524 Fragments arranged by size: 1524 787 747 597 584 483 407 393 268 178 158 68 17 EcoO109I rG'GnC_Cy Cuts at: 2536 2536 Size: 6211 EcoRI G'AATT_C Cuts at: 2448 3399 3520 3898 2448 Size: 951 121 378 4761 Fragments arranged by size: 4761 951 378 121 EcoRV GAT'ATC Cuts at: 2464 2464 Size: 6211 FspI TGC'GCA Cuts at: 4717 4717 Size: 6211 HaeII r_GCGC'y Cuts at: 1678 5358 1678 Size: 3680 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3148 3180 3245 3277 4852 Size: 32 135 32 1576 32 65 32 1575 Cuts at: 4852 4884 4926 4958 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1576 1575 135 65 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3174 3555 3801 669 Size: 988 281 1236 381 246 3079 Fragments arranged by size: 3079 1236 988 381 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6211 HpaI GTT'AAC Cuts at: 3174 3801 3174 Size: 627 5584 KpnI G_GTAC'C Cuts at: 3536 3536 Size: 6211 MfeI C'AATT_G Cuts at: 3810 3810 Size: 6211 MluI A'CGCG_T Cuts at: 6087 6087 Size: 6211 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2528 5325 5509 1305 Size: 1223 2797 184 2007 Fragments arranged by size: 2797 2007 1223 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4389 Fragments arranged by size: 4389 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3252 4169 4528 4687 518 Size: 648 2086 917 359 159 2042 Fragments arranged by size: 2086 2042 917 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2700 3314 3627 4304 5452 5697 1606 Size: 1094 614 313 677 1148 245 2120 Fragments arranged by size: 2120 1148 1094 677 614 313 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2840 513 Size: 552 939 836 3884 Fragments arranged by size: 3884 939 836 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6211 NheI G'CTAG_C Cuts at: 1052 2075 1052 Size: 1023 5188 NotI GC'GGCC_GC Cuts at: 3658 3658 Size: 6211 NsiI A_TGCA'T Cuts at: 3145 3145 Size: 6211 NspI r_CATG'y Cuts at: 1132 1229 3453 3569 5114 1132 Size: 97 2224 116 1545 2229 Fragments arranged by size: 2229 2224 1545 116 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6211 PciI A'CATG_T Cuts at: 5110 5110 Size: 6211 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2101 2879 1108 Size: 902 91 778 4440 Fragments arranged by size: 4440 902 778 91 PfoI T'CCnGG_A Cuts at: 2084 2084 Size: 6211 PmeI GTTT'AAAC Cuts at: 3515 3515 Size: 6211 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4280 4312 5793 5825 4280 Size: 32 1481 32 4666 Fragments arranged by size: 4666 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2536 2536 Size: 6211 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6211 PsiI TTA'TAA Cuts at: 3781 3781 Size: 6211 PstI C_TGCA'G Cuts at: 830 3121 3204 3563 830 Size: 2291 83 359 3478 Fragments arranged by size: 3478 2291 359 83 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6211 PvuII CAG'CTG Cuts at: 2700 3314 2700 Size: 614 5597 SalI G'TCGA_C Cuts at: 1936 3553 1936 Size: 1617 4594 SanDI GG'GwC_CC Cuts at: 2536 2536 Size: 6211 SbfI CC_TGCA'GG Cuts at: 3563 3563 Size: 6211 ScaI AGT'ACT Cuts at: 4459 6171 4459 Size: 1712 4499 SfcI C'TryA_G Cuts at: 826 1045 2116 3117 3200 3559 4694 5375 5566 Size: 219 1071 1001 83 359 1135 681 191 Cuts at: 5566 826 Size: 1471 Fragments arranged by size: 1471 1135 1071 1001 681 359 219 191 83 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6211 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6211 SmaI CCC'GGG Cuts at: 1872 3538 1872 Size: 1666 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4315 5216 5478 820 Size: 197 926 2372 901 262 1553 Fragments arranged by size: 2372 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4002 493 Size: 3509 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6211 SphI G_CATG'C Cuts at: 1132 3569 1132 Size: 2437 3774 SspI AAT'ATT Cuts at: 5 52 4135 5 Size: 47 4083 2081 Fragments arranged by size: 4083 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6211 StyI C'CwwG_G Cuts at: 513 1065 2004 2492 2840 3167 3650 513 Size: 552 939 488 348 327 483 3074 Fragments arranged by size: 3074 939 552 488 483 348 327 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2093 4238 4255 4408 4593 5038 325 Size: 1255 513 2145 17 153 185 445 1498 Fragments arranged by size: 2145 1498 1255 513 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2394 2635 4457 Size: 274 80 33 51 1443 417 241 1822 Cuts at: 4457 6169 96 Size: 1712 138 Fragments arranged by size: 1822 1712 1443 417 274 241 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2516 2548 2593 2625 3390 3422 6171 6203 2516 Size: 32 45 32 765 32 2749 32 2524 Fragments arranged by size: 2749 2524 765 45 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6211 XbaI T'CTAG_A Cuts at: 3298 3547 3298 Size: 249 5962 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6211 XmaI C'CCGG_G Cuts at: 1870 3536 1870 Size: 1666 4545 XmnI GAAnn'nnTTC Cuts at: 2926 4340 2926 Size: 1414 4797 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4019 276 Size: 53 83 186 936 2485 2468 Fragments arranged by size: 2485 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI