(Circular) (Six-base) MAPSORT of: pFN21AB8379.seq Check: 628 from: 1 to: 6547 pFN21AB8379 6547 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2742 3061 2742 Size: 319 6228 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4357 278 Size: 53 83 186 936 2821 2468 Fragments arranged by size: 2821 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2277 3372 3890 1937 Size: 340 1095 518 4594 Fragments arranged by size: 4594 1095 518 340 Acc65I G'GTAC_C Cuts at: 3868 3868 Size: 6547 AclI AA'CG_TT Cuts at: 1563 4674 5047 1563 Size: 3111 373 3063 Fragments arranged by size: 3111 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2183 3118 4610 5994 6262 1632 Size: 551 935 1492 1384 268 1917 Fragments arranged by size: 1917 1492 1384 935 551 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6350 AflIII A'CryG_T Cuts at: 1203 2397 2490 5446 6423 1203 Size: 1194 93 2956 977 1327 Fragments arranged by size: 2956 1327 1194 977 93 AgeI A'CCGG_T Cuts at: 5378 5378 Size: 6547 AhdI GACnn_n'nnGTC Cuts at: 3148 5276 3148 Size: 2128 4419 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6547 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2750 2802 1184 Size: 1566 52 4929 Fragments arranged by size: 4929 1566 52 AlwNI CAG_nnn'CTG Cuts at: 2023 2629 2938 3076 3523 4290 5862 2023 Size: 606 309 138 447 767 1572 2708 Fragments arranged by size: 2708 1572 767 606 447 309 138 ApaI G_GGCC'C Cuts at: 2180 3127 2180 Size: 947 5600 ApaLI G'TGCA_C Cuts at: 3791 4604 5760 3791 Size: 813 1156 4578 Fragments arranged by size: 4578 1156 813 ApoI r'AATT_y Cuts at: 1493 3856 4082 4234 6343 1493 Size: 2363 226 152 2109 1697 Fragments arranged by size: 2363 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5101 160 Size: 872 4069 1606 Fragments arranged by size: 4069 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6547 AvaI C'yCGr_G Cuts at: 1870 1943 2171 2599 3260 3366 3573 3872 1870 Size: 73 228 428 661 106 207 299 4545 Fragments arranged by size: 4545 661 428 299 228 207 106 73 BamHI G'GATC_C Cuts at: 3465 3559 3877 4244 3465 Size: 94 318 367 5768 Fragments arranged by size: 5768 367 318 94 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2342 2786 3362 3430 3868 Size: 325 217 627 555 444 576 68 438 Cuts at: 3868 5323 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 576 555 444 438 325 217 68 BanII G_rGCy'C Cuts at: 1391 2180 2253 3104 3127 3573 3619 3782 3866 Size: 789 73 851 23 446 46 163 84 Cuts at: 3866 1391 Size: 4072 Fragments arranged by size: 4072 851 789 446 163 84 73 46 23 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2171 2971 928 Size: 971 272 800 4504 Fragments arranged by size: 4504 971 800 272 BbvCI CC'TCA_GC Cuts at: 2097 2097 Size: 6547 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4738 4772 1903 Size: 34 2801 34 3678 Fragments arranged by size: 3678 2801 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4439 5649 4439 Size: 1210 5337 BclI T'GATC_A Cuts at: 1548 1548 Size: 6547 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3338 5158 136 Size: 107 122 71 631 605 1666 1820 1525 Fragments arranged by size: 1820 1666 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6542 6542 Size: 6547 BlpI GC'TnA_GC Cuts at: 2372 3618 3716 3964 2372 Size: 1246 98 248 4955 Fragments arranged by size: 4955 1246 248 98 Bme1580I G_kGCm'C Cuts at: 1790 2180 2345 2791 3127 3326 3367 3435 3795 Size: 390 165 446 336 199 41 68 360 Cuts at: 3795 4608 5764 1790 Size: 813 1156 2573 Fragments arranged by size: 2573 1156 813 446 390 360 336 199 165 68 41 BmgBI CAC'GTC Cuts at: 1318 2491 1318 Size: 1173 5374 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3544 4278 5236 Size: 416 53 83 399 24 2127 734 958 Cuts at: 5236 442 Size: 1753 Fragments arranged by size: 2127 1753 958 734 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6547 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3035 3035 Size: 6547 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2856 3671 3796 5207 1239 Size: 1617 815 125 1411 2579 Fragments arranged by size: 2579 1617 1411 815 125 Bpu10I CC'TnA_GC Cuts at: 2097 3290 2097 Size: 1193 5354 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2952 4672 5537 5835 2952 Size: 1720 865 298 3664 Fragments arranged by size: 3664 1720 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2618 3570 5210 882 Size: 617 1119 952 1640 2219 Fragments arranged by size: 2219 1640 1119 952 617 BsaAI yAC'GTr Cuts at: 493 1204 2959 4338 493 Size: 711 1755 1379 2702 Fragments arranged by size: 2702 1755 1379 711 BsaBI GATnn'nnATC Cuts at: 6541 6541 Size: 6547 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4354 4736 5371 275 Size: 53 83 186 936 2821 382 635 1451 Fragments arranged by size: 2821 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2245 2982 4980 5378 5652 5799 6210 2245 Size: 737 1998 398 274 147 411 2582 Fragments arranged by size: 2582 1998 737 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2043 2073 3737 3767 1178 Size: 30 165 30 640 30 1664 30 3958 Fragments arranged by size: 3958 1664 640 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2051 2054 2057 2100 2470 3515 1186 Size: 865 3 3 43 370 1045 4218 Fragments arranged by size: 4218 1045 865 370 43 3 3 BseYI C'CCAG_C Cuts at: 2747 3333 3341 3516 5750 6246 2747 Size: 586 8 175 2234 496 3048 Fragments arranged by size: 3048 2234 586 496 175 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2012 2666 1163 Size: 573 276 654 5044 Fragments arranged by size: 5044 654 573 276 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3647 3997 4758 5786 1540 Size: 275 186 1646 350 761 1028 2301 Fragments arranged by size: 2301 1646 1028 761 350 275 186 BsiHKAI G_wGCw'C Cuts at: 3782 3795 4608 4693 5764 3782 Size: 13 813 85 1071 4565 Fragments arranged by size: 4565 1071 813 85 13 BsmI GAATG_Cn' Cuts at: 2154 4058 4151 2154 Size: 1904 93 4550 Fragments arranged by size: 4550 1904 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2180 2253 2345 2791 3104 3127 3326 Size: 399 390 73 92 446 313 23 199 Cuts at: 3326 3367 3435 3573 3619 3782 3795 3866 4608 Size: 41 68 138 46 163 13 71 742 Cuts at: 4608 4693 5764 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 446 399 390 313 199 163 138 92 85 73 71 68 46 41 23 13 BspEI T'CCGG_A Cuts at: 6210 6210 Size: 6547 BspHI T'CATG_A Cuts at: 4436 6166 4436 Size: 1730 4817 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2742 2923 3061 3648 3902 844 Size: 1898 181 138 587 254 3489 Fragments arranged by size: 3489 1898 587 254 181 138 BsrBI CCG'CTC Cuts at: 1394 1823 2784 4434 1394 Size: 429 961 1650 3507 Fragments arranged by size: 3507 1650 961 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2611 5042 5216 65 Size: 1183 1363 2431 174 1396 Fragments arranged by size: 2431 1396 1363 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2142 5191 5378 1700 Size: 110 332 3049 187 2869 Fragments arranged by size: 3049 2869 332 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6547 BssHII G'CGCG_C Cuts at: 1925 3670 1925 Size: 1745 4802 BssSI C'ACGA_G Cuts at: 4607 5619 4607 Size: 1012 5535 BstAPI GCAn_nnn'nTGC Cuts at: 794 2023 2644 2962 794 Size: 1229 621 318 4379 Fragments arranged by size: 4379 1229 621 318 BstBI TT'CG_AA Cuts at: 5351 5351 Size: 6547 BstEII G'GTnAC_C Cuts at: 1184 2672 1184 Size: 1488 5059 BstXI CCAn_nnnn'nTGG Cuts at: 2848 3341 2848 Size: 493 6054 BstYI r'GATC_y Cuts at: 1972 2448 3465 3559 3877 4244 4629 4646 6098 Size: 476 1017 94 318 367 385 17 1452 Cuts at: 6098 6184 6196 6542 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1017 476 385 367 346 318 94 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3278 3278 Size: 6547 BtgI C'CryG_G Cuts at: 513 1065 2220 2808 3061 3327 3436 513 Size: 552 1155 588 253 266 109 3624 Fragments arranged by size: 3624 1155 588 552 266 253 109 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3309 487 Size: 683 56 2083 3725 Fragments arranged by size: 3725 2083 683 56 BtsI GCAGTG_nn' Cuts at: 2024 2645 4063 4857 4877 2024 Size: 621 1418 794 20 3694 Fragments arranged by size: 3694 1418 794 621 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6547 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6512 DraI TTT'AAA Cuts at: 3851 3851 Size: 6547 DraIII CAC_nnn'GTG Cuts at: 2860 2860 Size: 6547 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3314 5554 809 Size: 507 1998 2240 1802 Fragments arranged by size: 2240 1998 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2500 3177 3644 Size: 54 1425 50 275 18 670 677 467 Cuts at: 3644 3994 4883 6512 8 Size: 350 889 1629 43 Fragments arranged by size: 1629 1425 889 677 670 467 350 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3644 3994 1537 Size: 275 1832 350 4090 Fragments arranged by size: 4090 1832 350 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4477 1353 Size: 3124 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3758 5134 5508 5654 387 Size: 1064 138 2169 1376 374 146 1280 Fragments arranged by size: 2169 1376 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3780 3780 Size: 6547 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2183 2856 3118 3671 3796 4610 5207 Size: 393 551 673 262 553 125 814 597 Cuts at: 5207 5994 6262 1239 Size: 787 268 1524 Fragments arranged by size: 1524 814 787 673 597 553 551 393 268 262 125 EcoNI CCTnn'n_nnAGG Cuts at: 2265 3281 2265 Size: 1016 5531 EcoO109I rG'GnC_Cy Cuts at: 2010 2176 2177 2225 2475 2596 2755 2935 3037 Size: 166 1 48 250 121 159 180 102 Cuts at: 3037 3123 3124 3265 3286 3586 2010 Size: 86 1 141 21 300 4971 Fragments arranged by size: 4971 300 250 180 166 159 141 121 102 86 48 21 1 1 EcoRI G'AATT_C Cuts at: 3856 4234 3856 Size: 378 6169 FspI TGC'GCA Cuts at: 5053 5053 Size: 6547 HaeII r_GCGC'y Cuts at: 1678 3723 3751 5694 1678 Size: 2045 28 1943 2531 Fragments arranged by size: 2531 2045 1943 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2666 2698 3086 3118 3134 Size: 32 135 32 1094 32 388 32 16 Cuts at: 3134 3166 3821 3853 5188 5220 5262 5294 1373 Size: 32 655 32 1335 32 42 32 2626 Fragments arranged by size: 2626 1335 1094 655 388 135 42 32 32 32 32 32 32 32 32 16 HincII GTy'rAC Cuts at: 669 1657 1938 3891 4137 669 Size: 988 281 1953 246 3079 Fragments arranged by size: 3079 1953 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6547 HpaI GTT'AAC Cuts at: 4137 4137 Size: 6547 KpnI G_GTAC'C Cuts at: 3872 3872 Size: 6547 MfeI C'AATT_G Cuts at: 4146 4146 Size: 6547 MluI A'CGCG_T Cuts at: 6423 6423 Size: 6547 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5661 5845 1305 Size: 4356 184 2007 Fragments arranged by size: 4356 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4725 Fragments arranged by size: 4725 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2348 4505 4864 5023 518 Size: 648 1182 2157 359 159 2042 Fragments arranged by size: 2157 2042 1182 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2848 3337 3649 3774 3963 4640 5788 6033 Size: 1242 489 312 125 189 677 1148 245 Cuts at: 6033 1606 Size: 2120 Fragments arranged by size: 2120 1242 1148 677 489 312 245 189 125 NaeI GCC'GGC Cuts at: 2144 2144 Size: 6547 NcoI C'CATG_G Cuts at: 513 1065 2220 513 Size: 552 1155 4840 Fragments arranged by size: 4840 1155 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6547 NgoMIV G'CCGG_C Cuts at: 2142 2142 Size: 6547 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6547 NotI GC'GGCC_GC Cuts at: 3644 3994 3644 Size: 350 6197 NspI r_CATG'y Cuts at: 1132 1229 2731 3905 5450 1132 Size: 97 1502 1174 1545 2229 Fragments arranged by size: 2229 1545 1502 1174 97 PasI CC'CwG_GG Cuts at: 1348 2802 3358 1348 Size: 1454 556 4537 Fragments arranged by size: 4537 1454 556 PciI A'CATG_T Cuts at: 5446 5446 Size: 6547 PflMI CCAn_nnn'nTGG Cuts at: 1108 2314 2860 1108 Size: 1206 546 4795 Fragments arranged by size: 4795 1206 546 PfoI T'CCnGG_A Cuts at: 2616 2616 Size: 6547 PmeI GTTT'AAAC Cuts at: 3851 3851 Size: 6547 PmlI CAC'GTG Cuts at: 2959 2959 Size: 6547 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3437 3469 4616 4648 6129 6161 3437 Size: 32 1147 32 1481 32 3823 Fragments arranged by size: 3823 1481 1147 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2225 2935 3265 2225 Size: 710 330 5507 Fragments arranged by size: 5507 710 330 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6547 PsiI TTA'TAA Cuts at: 4117 4117 Size: 6547 PspOMI G'GGCC_C Cuts at: 2176 3123 2176 Size: 947 5600 PstI C_TGCA'G Cuts at: 830 2640 2781 3072 3899 830 Size: 1810 141 291 827 3478 Fragments arranged by size: 3478 1810 827 291 141 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6547 PvuII CAG'CTG Cuts at: 2848 3337 2848 Size: 489 6058 SacI G_AGCT'C Cuts at: 3782 3782 Size: 6547 SalI G'TCGA_C Cuts at: 1936 3889 1936 Size: 1953 4594 SanDI GG'GwC_CC Cuts at: 2225 2225 Size: 6547 SbfI CC_TGCA'GG Cuts at: 3072 3899 3072 Size: 827 5720 ScaI AGT'ACT Cuts at: 4795 6507 4795 Size: 1712 4835 SexAI A'CCwGG_T Cuts at: 3843 3843 Size: 6547 SfcI C'TryA_G Cuts at: 826 1045 2467 2636 2777 3068 3817 3895 5030 Size: 219 1422 169 141 291 749 78 1135 Cuts at: 5030 5711 5902 826 Size: 681 191 1471 Fragments arranged by size: 1471 1422 1135 749 681 291 219 191 169 141 78 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6547 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6547 SmaI CCC'GGG Cuts at: 1872 2173 2601 3368 3874 1872 Size: 301 428 767 506 4545 Fragments arranged by size: 4545 767 506 428 301 SmlI C'TyrA_G Cuts at: 820 1017 1943 2967 4651 5552 5814 820 Size: 197 926 1024 1684 901 262 1553 Fragments arranged by size: 1684 1553 1024 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4338 493 Size: 3845 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6547 SphI G_CATG'C Cuts at: 1132 2731 3905 1132 Size: 1599 1174 3774 Fragments arranged by size: 3774 1599 1174 SspI AAT'ATT Cuts at: 5 52 4471 5 Size: 47 4419 2081 Fragments arranged by size: 4419 2081 47 StuI AGG'CCT Cuts at: 1517 3680 1517 Size: 2163 4384 StyI C'CwwG_G Cuts at: 513 1065 2220 2261 2484 3582 3986 513 Size: 552 1155 41 223 1098 404 3074 Fragments arranged by size: 3074 1155 1098 552 404 223 41 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2069 2233 2632 3071 4574 4591 4744 Size: 1255 489 164 399 439 1503 17 153 Cuts at: 4744 4929 5374 325 Size: 185 445 1498 Fragments arranged by size: 1503 1498 1255 489 445 439 399 185 164 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4793 6505 96 Size: 274 80 33 51 1443 2816 1712 138 Fragments arranged by size: 2816 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2021 2043 2053 2075 2723 2755 3383 3415 3544 Size: 22 10 22 648 32 628 32 129 Cuts at: 3544 3576 3737 3769 6507 6539 2021 Size: 32 161 32 2738 32 2029 Fragments arranged by size: 2738 2029 648 628 161 129 32 32 32 32 32 22 22 10 Tth111I GACn'n_nGTC Cuts at: 1316 2229 1316 Size: 913 5634 XbaI T'CTAG_A Cuts at: 3883 3883 Size: 6547 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2815 2815 Size: 6547 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6547 XmaI C'CCGG_G Cuts at: 1870 2171 2599 3366 3872 1870 Size: 301 428 767 506 4545 Fragments arranged by size: 4545 767 506 428 301 XmnI GAAnn'nnTTC Cuts at: 3509 3698 3806 4676 3509 Size: 189 108 870 5380 Fragments arranged by size: 5380 870 189 108 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4355 276 Size: 53 83 186 936 2821 2468 Fragments arranged by size: 2821 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacII SapI SfiI SfoI SrfI SwaI