(Circular) (Six-base) MAPSORT of: pFN21AB8378.seq Check: 3583 from: 1 to: 6379 pFN21AB8378 6379 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2109 2167 3583 2109 Size: 58 1416 4905 Fragments arranged by size: 4905 1416 58 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4189 278 Size: 53 83 186 936 2653 2468 Fragments arranged by size: 2653 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3722 1937 Size: 1785 4594 Acc65I G'GTAC_C Cuts at: 3700 3700 Size: 6379 AclI AA'CG_TT Cuts at: 1563 4506 4879 1563 Size: 2943 373 3063 Fragments arranged by size: 3063 2943 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2293 2503 3041 4442 5826 6094 1632 Size: 661 210 538 1401 1384 268 1917 Fragments arranged by size: 1917 1401 1384 661 538 268 210 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6182 AflIII A'CryG_T Cuts at: 1203 3268 5278 6255 1203 Size: 2065 2010 977 1327 Fragments arranged by size: 2065 2010 1327 977 AgeI A'CCGG_T Cuts at: 3676 5210 3676 Size: 1534 4845 AhdI GACnn_n'nnGTC Cuts at: 5108 5108 Size: 6379 AleI CACnn'nnGTG Cuts at: 1166 3239 1166 Size: 2073 4306 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6379 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2963 2995 2963 Size: 32 6347 AlwNI CAG_nnn'CTG Cuts at: 2269 3451 4122 5694 2269 Size: 1182 671 1572 2954 Fragments arranged by size: 2954 1572 1182 671 ApaI G_GGCC'C Cuts at: 2301 2301 Size: 6379 ApaLI G'TGCA_C Cuts at: 2347 4436 5592 2347 Size: 2089 1156 3134 Fragments arranged by size: 3134 2089 1156 ApoI r'AATT_y Cuts at: 1493 2735 3688 3914 4066 6175 1493 Size: 1242 953 226 152 2109 1697 Fragments arranged by size: 2109 1697 1242 953 226 152 AseI AT'TA_AT Cuts at: 160 1032 4933 160 Size: 872 3901 1606 Fragments arranged by size: 3901 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6379 AvaI C'yCGr_G Cuts at: 1870 1943 2042 2455 2709 3628 3704 1870 Size: 73 99 413 254 919 76 4545 Fragments arranged by size: 4545 919 413 254 99 76 73 BamHI G'GATC_C Cuts at: 3102 3709 4076 3102 Size: 607 367 5405 Fragments arranged by size: 5405 607 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2097 3244 3700 5155 618 Size: 325 217 627 310 1147 456 1455 1842 Fragments arranged by size: 1842 1455 1147 627 456 325 310 217 BanII G_rGCy'C Cuts at: 1391 2018 2301 2481 2547 3100 3495 3537 3698 Size: 627 283 180 66 553 395 42 161 Cuts at: 3698 1391 Size: 4072 Fragments arranged by size: 4072 627 553 395 283 180 161 66 42 BbeI G_GCGC'C Cuts at: 3248 3248 Size: 6379 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2224 928 Size: 971 325 5083 Fragments arranged by size: 5083 971 325 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4570 4604 1903 Size: 34 2633 34 3678 Fragments arranged by size: 3678 2633 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4271 5481 4271 Size: 1210 5169 BclI T'GATC_A Cuts at: 1548 1548 Size: 6379 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3618 3656 4990 Size: 107 122 71 631 605 1946 38 1334 Cuts at: 4990 136 Size: 1525 Fragments arranged by size: 1946 1525 1334 631 605 122 107 71 38 BglII A'GATC_T Cuts at: 3045 6374 3045 Size: 3329 3050 BlpI GC'TnA_GC Cuts at: 2087 3796 2087 Size: 1709 4670 Bme1580I G_kGCm'C Cuts at: 1790 2301 2351 3525 4440 5596 1790 Size: 511 50 1174 915 1156 2573 Fragments arranged by size: 2573 1174 1156 915 511 50 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6379 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2782 4110 5068 Size: 416 53 83 399 24 1365 1328 958 Cuts at: 5068 442 Size: 1753 Fragments arranged by size: 1753 1365 1328 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6379 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2470 2470 Size: 6379 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2561 5039 1239 Size: 1322 2478 2579 Fragments arranged by size: 2579 2478 1322 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2384 2852 3521 4504 5369 5667 2384 Size: 468 669 983 865 298 3096 Fragments arranged by size: 3096 983 865 669 468 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2955 5042 882 Size: 617 1456 2087 2219 Fragments arranged by size: 2219 2087 1456 617 BsaAI yAC'GTr Cuts at: 493 1204 2473 4170 493 Size: 711 1269 1697 2702 Fragments arranged by size: 2702 1697 1269 711 BsaBI GATnn'nnATC Cuts at: 6373 6373 Size: 6379 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3245 3468 4186 4568 Size: 53 83 186 936 1712 223 718 382 Cuts at: 4568 5203 275 Size: 635 1451 Fragments arranged by size: 1712 1451 936 718 635 382 223 186 83 53 BsaWI w'CCGG_w Cuts at: 2199 3676 4812 5210 5484 5631 6042 2199 Size: 1477 1136 398 274 147 411 2536 Fragments arranged by size: 2536 1477 1136 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2838 2868 2963 2993 1178 Size: 30 165 30 1435 30 95 30 4564 Fragments arranged by size: 4564 1435 165 95 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2270 2681 2768 2789 3299 1186 Size: 1084 411 87 21 510 4266 Fragments arranged by size: 4266 1084 510 411 87 21 BseYI C'CCAG_C Cuts at: 2135 2300 5582 6078 2135 Size: 165 3282 496 2436 Fragments arranged by size: 3282 2436 496 165 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2195 2282 2969 3655 1163 Size: 573 459 87 687 686 3887 Fragments arranged by size: 3887 687 686 573 459 87 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3282 3829 4590 5618 1540 Size: 275 186 1281 547 761 1028 2301 Fragments arranged by size: 2301 1281 1028 761 547 275 186 BsiHKAI G_wGCw'C Cuts at: 2351 2481 2547 3293 3537 4440 4525 5596 2351 Size: 130 66 746 244 903 85 1071 3134 Fragments arranged by size: 3134 1071 903 746 244 130 85 66 BsmI GAATG_Cn' Cuts at: 3890 3983 3890 Size: 93 6286 BsmBI CGTCTCn'nnnn_ Cuts at: 2818 2818 Size: 6379 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2018 2301 2351 2481 2547 3100 3293 Size: 399 228 283 50 130 66 553 193 Cuts at: 3293 3495 3525 3537 3698 4440 4525 5596 1391 Size: 202 30 12 161 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 553 399 283 228 202 193 161 130 85 66 50 30 12 BspEI T'CCGG_A Cuts at: 2199 6042 2199 Size: 3843 2536 BspHI T'CATG_A Cuts at: 4268 5998 4268 Size: 1730 4649 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2109 2167 3436 3583 3734 844 Size: 1265 58 1269 147 151 3489 Fragments arranged by size: 3489 1269 1265 151 147 58 BsrBI CCG'CTC Cuts at: 1394 1823 2119 4266 1394 Size: 429 296 2147 3507 Fragments arranged by size: 3507 2147 429 296 BsrDI GCAATG_nn' Cuts at: 65 1248 4874 5048 65 Size: 1183 3626 174 1396 Fragments arranged by size: 3626 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2094 3676 5023 5210 1700 Size: 110 284 1582 1347 187 2869 Fragments arranged by size: 2869 1582 1347 284 187 110 BsrGI T'GTAC_A Cuts at: 96 2583 96 Size: 2487 3892 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6379 BssSI C'ACGA_G Cuts at: 4439 5451 4439 Size: 1012 5367 BstAPI GCAn_nnn'nTGC Cuts at: 794 2269 2346 3551 794 Size: 1475 77 1205 3622 Fragments arranged by size: 3622 1475 1205 77 BstBI TT'CG_AA Cuts at: 5183 5183 Size: 6379 BstEII G'GTnAC_C Cuts at: 1184 2380 3343 1184 Size: 1196 963 4220 Fragments arranged by size: 4220 1196 963 BstXI CCAn_nnnn'nTGG Cuts at: 2500 3241 2500 Size: 741 5638 BstYI r'GATC_y Cuts at: 1972 2919 3045 3102 3709 4076 4461 4478 5930 Size: 947 126 57 607 367 385 17 1452 Cuts at: 5930 6016 6028 6374 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 947 607 385 367 346 126 86 57 17 12 Bsu36I CC'TnA_GG Cuts at: 3153 3153 Size: 6379 BtgI C'CryG_G Cuts at: 513 1065 2931 2966 3036 3482 513 Size: 552 1866 35 70 446 3410 Fragments arranged by size: 3410 1866 552 446 70 35 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5640 Fragments arranged by size: 5640 683 56 BtsI GCAGTG_nn' Cuts at: 3895 4689 4709 3895 Size: 794 20 5565 Fragments arranged by size: 5565 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6379 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6344 DraI TTT'AAA Cuts at: 3683 3683 Size: 6379 DraIII CAC_nnn'GTG Cuts at: 2881 3488 2881 Size: 607 5772 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3446 3467 5386 809 Size: 507 2130 21 1919 1802 Fragments arranged by size: 2130 1919 1802 507 21 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2934 3279 3354 Size: 54 1425 50 275 18 1104 345 75 Cuts at: 3354 3561 3576 3826 4715 6344 8 Size: 207 15 250 889 1629 43 Fragments arranged by size: 1629 1425 1104 889 345 275 250 207 75 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 3279 3826 1537 Size: 275 1467 547 4090 Fragments arranged by size: 4090 1467 547 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2522 2816 4309 1353 Size: 1169 294 1493 3423 Fragments arranged by size: 3423 1493 1169 294 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4966 5340 5486 387 Size: 1064 138 3377 374 146 1280 Fragments arranged by size: 3377 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2479 2545 3535 2479 Size: 66 990 5323 Fragments arranged by size: 5323 990 66 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2293 2503 2561 3041 4442 5039 5826 Size: 393 661 210 58 480 1401 597 787 Cuts at: 5826 6094 1239 Size: 268 1524 Fragments arranged by size: 1524 1401 787 661 597 480 393 268 210 58 EcoO109I rG'GnC_Cy Cuts at: 2512 3448 3583 2512 Size: 936 135 5308 Fragments arranged by size: 5308 936 135 EcoRI G'AATT_C Cuts at: 2735 3688 4066 2735 Size: 953 378 5048 Fragments arranged by size: 5048 953 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2533 2740 2533 Size: 207 6172 FspI TGC'GCA Cuts at: 4885 4885 Size: 6379 HaeII r_GCGC'y Cuts at: 1678 3248 3560 5526 1678 Size: 1570 312 1966 2531 Fragments arranged by size: 2531 1966 1570 312 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2662 2690 2694 2722 2838 Size: 32 135 32 1090 28 4 28 116 Cuts at: 2838 2870 3087 3119 5020 5052 5094 5126 1373 Size: 32 217 32 1901 32 42 32 2626 Fragments arranged by size: 2626 1901 1090 217 135 116 42 32 32 32 32 32 32 28 28 4 HincII GTy'rAC Cuts at: 669 1657 1938 2557 3723 3969 669 Size: 988 281 619 1166 246 3079 Fragments arranged by size: 3079 1166 988 619 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6379 HpaI GTT'AAC Cuts at: 3969 3969 Size: 6379 KasI G'GCGC_C Cuts at: 3244 3244 Size: 6379 KpnI G_GTAC'C Cuts at: 3704 3704 Size: 6379 MfeI C'AATT_G Cuts at: 3978 3978 Size: 6379 MluI A'CGCG_T Cuts at: 3268 6255 3268 Size: 2987 3392 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2154 2553 5493 5677 1305 Size: 849 399 2940 184 2007 Fragments arranged by size: 2940 2007 849 399 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2936 3563 10 Size: 54 1425 343 1104 627 2826 Fragments arranged by size: 2826 1425 1104 627 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2435 3239 3410 3440 4337 4696 4855 Size: 648 1269 804 171 30 897 359 159 Cuts at: 4855 518 Size: 2042 Fragments arranged by size: 2042 1269 897 804 648 359 171 159 30 MspA1I CmG'CkG Cuts at: 1606 2091 2304 2313 2392 2578 2661 2968 2976 Size: 485 213 9 79 186 83 307 8 Cuts at: 2976 3795 4472 5620 5865 1606 Size: 819 677 1148 245 2120 Fragments arranged by size: 2120 1148 819 677 485 307 245 213 186 83 79 9 8 NaeI GCC'GGC Cuts at: 2096 2096 Size: 6379 NarI GG'CG_CC Cuts at: 3245 3245 Size: 6379 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5827 NdeI CA'TA_TG Cuts at: 387 387 Size: 6379 NgoMIV G'CCGG_C Cuts at: 2094 2094 Size: 6379 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6379 NotI GC'GGCC_GC Cuts at: 3826 3826 Size: 6379 NspI r_CATG'y Cuts at: 1132 1229 3445 3737 5282 1132 Size: 97 2216 292 1545 2229 Fragments arranged by size: 2229 2216 1545 292 97 PasI CC'CwG_GG Cuts at: 1348 2353 3213 1348 Size: 1005 860 4514 Fragments arranged by size: 4514 1005 860 PciI A'CATG_T Cuts at: 5278 5278 Size: 6379 PflMI CCAn_nnn'nTGG Cuts at: 1108 3184 1108 Size: 2076 4303 PfoI T'CCnGG_A Cuts at: 2538 2892 3450 3501 3528 2538 Size: 354 558 51 27 5389 Fragments arranged by size: 5389 558 354 51 27 PmeI GTTT'AAAC Cuts at: 3683 3683 Size: 6379 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2963 2995 4448 4480 5961 5993 2963 Size: 32 1453 32 1481 32 3349 Fragments arranged by size: 3349 1481 1453 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2512 3448 3583 2512 Size: 936 135 5308 Fragments arranged by size: 5308 936 135 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6379 PsiI TTA'TAA Cuts at: 2835 3949 2835 Size: 1114 5265 PspOMI G'GGCC_C Cuts at: 2297 2297 Size: 6379 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2453 2485 2795 2827 2453 Size: 32 310 32 6005 Fragments arranged by size: 6005 310 32 32 PstI C_TGCA'G Cuts at: 830 2154 2660 3731 830 Size: 1324 506 1071 3478 Fragments arranged by size: 3478 1324 1071 506 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6379 PvuII CAG'CTG Cuts at: 2091 2313 2578 2661 2976 2091 Size: 222 265 83 315 5494 Fragments arranged by size: 5494 315 265 222 83 SacI G_AGCT'C Cuts at: 2481 2547 3537 2481 Size: 66 990 5323 Fragments arranged by size: 5323 990 66 SacII CC_GC'GG Cuts at: 2969 2969 Size: 6379 SalI G'TCGA_C Cuts at: 1936 3721 1936 Size: 1785 4594 SbfI CC_TGCA'GG Cuts at: 3731 3731 Size: 6379 ScaI AGT'ACT Cuts at: 3605 4627 6339 3605 Size: 1022 1712 3645 Fragments arranged by size: 3645 1712 1022 SfcI C'TryA_G Cuts at: 826 1045 2150 2548 2656 3607 3727 4862 5543 Size: 219 1105 398 108 951 120 1135 681 Cuts at: 5543 5734 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1105 951 681 398 219 191 120 108 SfoI GGC'GCC Cuts at: 3246 3246 Size: 6379 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6379 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6379 SmaI CCC'GGG Cuts at: 1872 2044 3706 1872 Size: 172 1662 4545 Fragments arranged by size: 4545 1662 172 SmlI C'TyrA_G Cuts at: 820 1017 1943 2399 2709 2867 3536 4483 5384 Size: 197 926 456 310 158 669 947 901 Cuts at: 5384 5646 820 Size: 262 1553 Fragments arranged by size: 1553 947 926 901 669 456 310 262 197 158 SnaBI TAC'GTA Cuts at: 493 4170 493 Size: 3677 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6379 SphI G_CATG'C Cuts at: 1132 3737 1132 Size: 2605 3774 SspI AAT'ATT Cuts at: 5 52 4303 5 Size: 47 4251 2081 Fragments arranged by size: 4251 2081 47 StuI AGG'CCT Cuts at: 1517 2630 2677 2768 3194 3621 1517 Size: 1113 47 91 426 427 4275 Fragments arranged by size: 4275 1113 427 426 91 47 StyI C'CwwG_G Cuts at: 513 1065 2226 3258 3818 513 Size: 552 1161 1032 560 3074 Fragments arranged by size: 3074 1161 1032 560 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2161 3362 3629 4406 4423 4576 4761 Size: 1255 581 1201 267 777 17 153 185 Cuts at: 4761 5206 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1201 777 581 445 267 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2583 2883 3603 Size: 274 80 33 51 1443 606 300 720 Cuts at: 3603 4625 6337 96 Size: 1022 1712 138 Fragments arranged by size: 1712 1443 1022 720 606 300 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3149 3181 6339 6371 3149 Size: 32 3158 32 3157 Fragments arranged by size: 3158 3157 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3467 1316 Size: 2151 4228 XbaI T'CTAG_A Cuts at: 3715 3715 Size: 6379 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3409 3571 3409 Size: 162 6217 XhoI C'TCGA_G Cuts at: 1943 2709 1943 Size: 766 5613 XmaI C'CCGG_G Cuts at: 1870 2042 3704 1870 Size: 172 1662 4545 Fragments arranged by size: 4545 1662 172 XmnI GAAnn'nnTTC Cuts at: 4508 4508 Size: 6379 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4187 276 Size: 53 83 186 936 2653 2468 Fragments arranged by size: 2653 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I EcoNI EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI RsrII SanDI SapI SexAI SfiI SrfI SwaI