(Circular) (Six-base) MAPSORT of: pFN21AB8376.seq Check: 7980 from: 1 to: 6583 pFN21AB8376 6583 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2587 2587 Size: 6583 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2712 4393 278 Size: 53 83 186 936 1176 1681 2468 Fragments arranged by size: 2468 1681 1176 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3926 1937 Size: 1989 4594 Acc65I G'GTAC_C Cuts at: 3904 3904 Size: 6583 AclI AA'CG_TT Cuts at: 1563 4710 5083 1563 Size: 3147 373 3063 Fragments arranged by size: 3147 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4646 6030 6298 1632 Size: 3014 1384 268 1917 Fragments arranged by size: 3014 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6386 AflIII A'CryG_T Cuts at: 1203 5482 6459 1203 Size: 4279 977 1327 Fragments arranged by size: 4279 1327 977 AgeI A'CCGG_T Cuts at: 5414 5414 Size: 6583 AhdI GACnn_n'nnGTC Cuts at: 5312 5312 Size: 6583 AleI CACnn'nnGTG Cuts at: 1166 2070 2465 2591 1166 Size: 904 395 126 5158 Fragments arranged by size: 5158 904 395 126 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2327 2609 1184 Size: 1143 282 5158 Fragments arranged by size: 5158 1143 282 AlwNI CAG_nnn'CTG Cuts at: 3100 3295 4326 5898 3100 Size: 195 1031 1572 3785 Fragments arranged by size: 3785 1572 1031 195 ApaLI G'TGCA_C Cuts at: 4640 5796 4640 Size: 1156 5427 ApoI r'AATT_y Cuts at: 1493 3892 4118 4270 6379 1493 Size: 2399 226 152 2109 1697 Fragments arranged by size: 2399 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5137 160 Size: 872 4105 1606 Fragments arranged by size: 4105 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6583 AvaI C'yCGr_G Cuts at: 1870 1943 2055 2330 3155 3276 3908 1870 Size: 73 112 275 825 121 632 4545 Fragments arranged by size: 4545 825 632 275 121 112 73 BamHI G'GATC_C Cuts at: 3913 4280 3913 Size: 367 6216 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2072 2437 2485 2585 3488 Size: 325 217 627 285 365 48 100 903 Cuts at: 3488 3557 3703 3729 3904 5359 618 Size: 69 146 26 175 1455 1842 Fragments arranged by size: 1842 1455 903 627 365 325 285 217 175 146 100 69 48 26 BanII G_rGCy'C Cuts at: 1391 2553 2768 2964 3523 3641 3774 3902 1391 Size: 1162 215 196 559 118 133 128 4072 Fragments arranged by size: 4072 1162 559 215 196 133 128 118 BbeI G_GCGC'C Cuts at: 3492 3561 3733 3492 Size: 69 172 6342 Fragments arranged by size: 6342 172 69 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2215 2907 928 Size: 971 316 692 4604 Fragments arranged by size: 4604 971 692 316 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3388 3422 4774 4808 1903 Size: 34 1451 34 1352 34 3678 Fragments arranged by size: 3678 1451 1352 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4475 5685 4475 Size: 1210 5373 BclI T'GATC_A Cuts at: 1548 1548 Size: 6583 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2921 5194 136 Size: 107 122 71 631 605 1249 2273 1525 Fragments arranged by size: 2273 1525 1249 631 605 122 107 71 BglII A'GATC_T Cuts at: 6578 6578 Size: 6583 BlpI GC'TnA_GC Cuts at: 2315 2519 4000 2315 Size: 204 1481 4898 Fragments arranged by size: 4898 1481 204 Bme1580I G_kGCm'C Cuts at: 1790 2442 2588 2622 3706 4644 5800 1790 Size: 652 146 34 1084 938 1156 2573 Fragments arranged by size: 2573 1156 1084 938 652 146 34 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6583 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2077 4314 5272 Size: 416 53 83 399 24 660 2237 958 Cuts at: 5272 442 Size: 1753 Fragments arranged by size: 2237 1753 958 660 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3857 1056 Size: 2801 3782 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2758 3049 3813 2758 Size: 291 764 5528 Fragments arranged by size: 5528 764 291 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2894 2900 3155 3275 3521 5243 1239 Size: 1655 6 255 120 246 1722 2579 Fragments arranged by size: 2579 1722 1655 255 246 120 6 Bpu10I CC'TnA_GC Cuts at: 2514 3788 2514 Size: 1274 5309 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4708 5573 5871 4708 Size: 865 298 5420 Fragments arranged by size: 5420 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3037 5246 882 Size: 617 1538 2209 2219 Fragments arranged by size: 2219 2209 1538 617 BsaAI yAC'GTr Cuts at: 493 1204 2112 2773 4374 493 Size: 711 908 661 1601 2702 Fragments arranged by size: 2702 1601 908 711 661 BsaBI GATnn'nnATC Cuts at: 3587 6577 3587 Size: 2990 3593 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2709 2907 3489 3558 Size: 53 83 186 936 1176 198 582 69 Cuts at: 3558 3730 4390 4772 5407 275 Size: 172 660 382 635 1451 Fragments arranged by size: 1451 1176 936 660 635 582 382 198 186 172 83 69 53 BsaWI w'CCGG_w Cuts at: 5016 5414 5688 5835 6246 5016 Size: 398 274 147 411 5353 Fragments arranged by size: 5353 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3371 3401 1178 Size: 30 165 30 1968 30 4360 Fragments arranged by size: 4360 1968 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2734 2897 3239 3760 3863 1186 Size: 1548 163 342 521 103 3906 Fragments arranged by size: 3906 1548 521 342 163 103 BseYI C'CCAG_C Cuts at: 2335 2527 2542 2560 2647 3641 3754 5786 6282 Size: 192 15 18 87 994 113 2032 496 Cuts at: 6282 2335 Size: 2636 Fragments arranged by size: 2636 2032 994 496 192 113 87 18 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2287 2594 2615 3308 3451 1163 Size: 573 551 307 21 693 143 4295 Fragments arranged by size: 4295 693 573 551 307 143 21 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3147 4033 4794 5822 1540 Size: 275 186 1146 886 761 1028 2301 Fragments arranged by size: 2301 1146 1028 886 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2400 3047 3249 3414 4644 4729 5800 2400 Size: 647 202 165 1230 85 1071 3183 Fragments arranged by size: 3183 1230 1071 647 202 165 85 BsmI GAATG_Cn' Cuts at: 2935 4094 4187 2935 Size: 1159 93 5331 Fragments arranged by size: 5331 1159 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2122 3020 2122 Size: 898 5685 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2400 2442 2553 2588 2622 2768 2964 Size: 399 610 42 111 35 34 146 196 Cuts at: 2964 3047 3249 3414 3523 3641 3706 3774 3902 Size: 83 202 165 109 118 65 68 128 Cuts at: 3902 4644 4729 5800 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 610 399 202 196 165 146 128 118 111 109 85 83 68 65 42 35 34 BspEI T'CCGG_A Cuts at: 6246 6246 Size: 6583 BspHI T'CATG_A Cuts at: 4472 6202 4472 Size: 1730 4853 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2587 3938 844 Size: 1743 1351 3489 Fragments arranged by size: 3489 1743 1351 BsrBI CCG'CTC Cuts at: 1394 1823 3376 4470 1394 Size: 429 1553 1094 3507 Fragments arranged by size: 3507 1553 1094 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3358 5078 5252 65 Size: 1183 2110 1720 174 1396 Fragments arranged by size: 2110 1720 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2565 3142 5227 5414 1700 Size: 110 755 577 2085 187 2869 Fragments arranged by size: 2869 2085 755 577 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6583 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6583 BssSI C'ACGA_G Cuts at: 2249 2534 2623 3407 4643 5655 2249 Size: 285 89 784 1236 1012 3177 Fragments arranged by size: 3177 1236 1012 784 285 89 BstAPI GCAn_nnn'nTGC Cuts at: 794 2617 794 Size: 1823 4760 BstBI TT'CG_AA Cuts at: 2103 5387 2103 Size: 3284 3299 BstEII G'GTnAC_C Cuts at: 1184 2887 1184 Size: 1703 4880 BstXI CCAn_nnnn'nTGG Cuts at: 2434 3499 2434 Size: 1065 5518 BstYI r'GATC_y Cuts at: 1972 3096 3737 3913 4280 4665 4682 6134 6220 Size: 1124 641 176 367 385 17 1452 86 Cuts at: 6220 6232 6578 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1124 641 385 367 346 176 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2356 2955 3105 3327 3561 513 Size: 552 1291 599 150 222 234 3535 Fragments arranged by size: 3535 1291 599 552 234 222 150 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3330 487 Size: 683 56 2104 3740 Fragments arranged by size: 3740 2104 683 56 BtsI GCAGTG_nn' Cuts at: 2021 2603 3262 3296 4099 4893 4913 2021 Size: 582 659 34 803 794 20 3691 Fragments arranged by size: 3691 803 794 659 582 34 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6583 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6548 DraI TTT'AAA Cuts at: 3887 3887 Size: 6583 DraIII CAC_nnn'GTG Cuts at: 2617 2617 Size: 6583 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2837 5590 809 Size: 507 1521 2753 1802 Fragments arranged by size: 2753 1802 1521 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2638 2856 2922 Size: 54 1425 50 275 18 808 218 66 Cuts at: 2922 2938 3012 3144 3199 4030 4919 6548 8 Size: 16 74 132 55 831 889 1629 43 Fragments arranged by size: 1629 1425 889 831 808 275 218 132 74 66 55 54 50 43 18 16 EagI C'GGCC_G Cuts at: 1537 1812 3144 4030 1537 Size: 275 1332 886 4090 Fragments arranged by size: 4090 1332 886 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4513 1353 Size: 3160 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5170 5544 5690 387 Size: 1064 138 3581 374 146 1280 Fragments arranged by size: 3581 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2894 2900 3155 3275 3521 4646 5243 Size: 393 1262 6 255 120 246 1125 597 Cuts at: 5243 6030 6298 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1262 1125 787 597 393 268 255 246 120 6 EcoNI CCTnn'n_nnAGG Cuts at: 2846 2846 Size: 6583 EcoO109I rG'GnC_Cy Cuts at: 2284 2362 3448 2284 Size: 78 1086 5419 Fragments arranged by size: 5419 1086 78 EcoRI G'AATT_C Cuts at: 3892 4270 3892 Size: 378 6205 EcoRV GAT'ATC Cuts at: 2413 2413 Size: 6583 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2368 2368 Size: 6583 FspI TGC'GCA Cuts at: 5089 5089 Size: 6583 HaeII r_GCGC'y Cuts at: 1678 3492 3561 3733 5730 1678 Size: 1814 69 172 1997 2531 Fragments arranged by size: 2531 1997 1814 172 69 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3329 3361 5224 5256 5298 Size: 32 135 32 1757 32 1863 32 42 Cuts at: 5298 5330 1373 Size: 32 2626 Fragments arranged by size: 2626 1863 1757 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2419 3927 4173 669 Size: 988 281 481 1508 246 3079 Fragments arranged by size: 3079 1508 988 481 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6583 HpaI GTT'AAC Cuts at: 4173 4173 Size: 6583 KasI G'GCGC_C Cuts at: 3488 3557 3729 3488 Size: 69 172 6342 Fragments arranged by size: 6342 172 69 KpnI G_GTAC'C Cuts at: 3908 3908 Size: 6583 MfeI C'AATT_G Cuts at: 4182 4182 Size: 6583 MluI A'CGCG_T Cuts at: 6459 6459 Size: 6583 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3336 5697 5881 1305 Size: 2031 2361 184 2007 Fragments arranged by size: 2361 2031 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2940 3201 10 Size: 54 1425 343 1108 261 3392 Fragments arranged by size: 3392 1425 1108 343 261 54 MslI CAynn'nnrTG Cuts at: 518 1166 2070 2435 2465 2591 4541 4900 5059 Size: 648 904 365 30 126 1950 359 159 Cuts at: 5059 518 Size: 2042 Fragments arranged by size: 2042 1950 904 648 365 359 159 126 30 MspA1I CmG'CkG Cuts at: 1606 2162 2478 2555 2812 3133 3402 3999 4676 Size: 556 316 77 257 321 269 597 677 Cuts at: 4676 5824 6069 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 597 556 321 316 269 257 245 77 NaeI GCC'GGC Cuts at: 2567 3144 2567 Size: 577 6006 NarI GG'CG_CC Cuts at: 3489 3558 3730 3489 Size: 69 172 6342 Fragments arranged by size: 6342 172 69 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6031 NdeI CA'TA_TG Cuts at: 387 387 Size: 6583 NgoMIV G'CCGG_C Cuts at: 2565 3142 2565 Size: 577 6006 NheI G'CTAG_C Cuts at: 1052 3853 1052 Size: 2801 3782 NotI GC'GGCC_GC Cuts at: 4030 4030 Size: 6583 NspI r_CATG'y Cuts at: 1132 1229 3941 5486 1132 Size: 97 2712 1545 2229 Fragments arranged by size: 2712 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6583 PciI A'CATG_T Cuts at: 5482 5482 Size: 6583 PflMI CCAn_nnn'nTGG Cuts at: 1108 2647 1108 Size: 1539 5044 PfoI T'CCnGG_A Cuts at: 2142 2406 2844 2871 3252 2142 Size: 264 438 27 381 5473 Fragments arranged by size: 5473 438 381 264 27 PmeI GTTT'AAAC Cuts at: 3887 3887 Size: 6583 PmlI CAC'GTG Cuts at: 2112 2773 2112 Size: 661 5922 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3365 3397 4652 4684 6165 6197 3365 Size: 32 1255 32 1481 32 3751 Fragments arranged by size: 3751 1481 1255 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2284 2362 2284 Size: 78 6505 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6583 PsiI TTA'TAA Cuts at: 4153 4153 Size: 6583 PstI C_TGCA'G Cuts at: 830 2685 3132 3935 830 Size: 1855 447 803 3478 Fragments arranged by size: 3478 1855 803 447 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6583 PvuII CAG'CTG Cuts at: 2478 3133 2478 Size: 655 5928 SalI G'TCGA_C Cuts at: 1936 3925 1936 Size: 1989 4594 SbfI CC_TGCA'GG Cuts at: 3935 3935 Size: 6583 ScaI AGT'ACT Cuts at: 2348 3173 4831 6543 2348 Size: 825 1658 1712 2388 Fragments arranged by size: 2388 1712 1658 825 SfcI C'TryA_G Cuts at: 826 1045 2681 3128 3931 5066 5747 5938 826 Size: 219 1636 447 803 1135 681 191 1471 Fragments arranged by size: 1636 1471 1135 803 681 447 219 191 SfoI GGC'GCC Cuts at: 3490 3559 3731 3490 Size: 69 172 6342 Fragments arranged by size: 6342 172 69 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6583 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6583 SmaI CCC'GGG Cuts at: 1872 3910 1872 Size: 2038 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4687 5588 5850 820 Size: 197 926 2744 901 262 1553 Fragments arranged by size: 2744 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4374 493 Size: 3881 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6583 SphI G_CATG'C Cuts at: 1132 3941 1132 Size: 2809 3774 SspI AAT'ATT Cuts at: 5 52 4507 5 Size: 47 4455 2081 Fragments arranged by size: 4455 2081 47 StuI AGG'CCT Cuts at: 1517 2660 1517 Size: 1143 5440 StyI C'CwwG_G Cuts at: 513 1065 3825 4022 513 Size: 552 2760 197 3074 Fragments arranged by size: 3074 2760 552 197 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2252 2581 4610 4627 4780 4965 5410 Size: 1255 672 329 2029 17 153 185 445 Cuts at: 5410 325 Size: 1498 Fragments arranged by size: 2029 1498 1255 672 445 329 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2346 2382 3171 Size: 274 80 33 51 1443 369 36 789 Cuts at: 3171 3387 4829 6541 96 Size: 216 1442 1712 138 Fragments arranged by size: 1712 1443 1442 789 369 274 216 138 80 51 36 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6543 6575 6543 Size: 32 6551 Tth111I GACn'n_nGTC Cuts at: 1316 2906 1316 Size: 1590 4993 XbaI T'CTAG_A Cuts at: 3919 3919 Size: 6583 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6583 XmaI C'CCGG_G Cuts at: 1870 3908 1870 Size: 2038 4545 XmnI GAAnn'nnTTC Cuts at: 4712 4712 Size: 6583 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2710 4391 276 Size: 53 83 186 936 1176 1681 2468 Fragments arranged by size: 2468 1681 1176 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I Bsu36I EcoICRI FseI FspAI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI