Restriction map for
pFN21AB8374
##################################
# NO CUT ENZYMES ( 128 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BsaAI BseAI BsiWI
BspDI BspEI BspHI BspLU11I BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraIII
DsaI Eam1105I Eco81I EcoNI EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HgaI HindIII HpaI KasI
KpnI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AatII 1300 GACGTC GACGT^C
AccI 1339 GTCTAC GT^[AC][GT]AC
AcyI 1300 GACGTC G[GA]^CG[CT]C
AlwNI 1100 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 47 AGCGCT AGC^GCT
Asp700I 130 GAACCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1032 CTCGGG C^[CT]CG[GA]G
BsaBI 151 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1300 GACGTC G[GA]^CG[CT]C
BsaI 454 GAGACC GGTCTC(1/5)
BsiEI 717 CGGCCG CG[GA][CT]^CG
BsmBI 1298 GAGACG CGTCTC(1/5)
BsmI 1152 GAATGC GAATGC(1/-1)
BsoBI 1032 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1247 TGTACA T^GTACA
BspMI 1369 GCAGGT ACCTGC(4/8)
BsrBI 1519 CCGCTC CCGCTC(-3/-3)
BsrGI 1247 TGTACA T^GTACA
BstYI 617 AGATCC [GA]^GATC[CT]
DrdI 1480 GACTGGACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 717 CGGCCG C^GGCCG
EclXI 717 CGGCCG C^GGCCG
Eco47III 47 AGCGCT AGC^GCT
Eco52I 717 CGGCCG C^GGCCG
Hin1I 1300 GACGTC G[GA]^CG[CT]C
HincII 594 GTTGAC GT[CT]^[GA]AC
HindII 594 GTTGAC GT[CT]^[GA]AC
MamI 151 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 617 AGATCC [GA]^GATC[CT]
NspI 1211 ACATGC [GA]CATG^[CT]
SspBI 1247 TGTACA T^GTACA
XhoII 617 AGATCC [GA]^GATC[CT]
XmnI 130 GAACCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 634 GAATTT [GA]^AATT[CT]
1829 AAATTC
AlwI 618 GATCC GGATC(4/5)
156 GGATC
ApaI 1437 GGGCCC GGGCC^C
1706 GGGCCC
ApoI 634 GAATTT [GA]^AATT[CT]
1829 AAATTC
AspHI 258 GAGCAC G[AT]GC[AT]^C
343 GTGCTC
BalI 528 TGGCCA TGG^CCA
1667 TGGCCA
BfaI 31 CTAG C^TAG
220 CTAG
BlpI 25 GCTGAGC GC^T[ACGT]AGC
50 GCTTAGC
Bpu1102I 25 GCTGAGC GC^T[ACGT]AGC
50 GCTTAGC
BsgI 1106 CTGCAC GTGCAG(16/14)
1081 GTGCAG
BsiHKAI 258 GAGCAC G[AT]GC[AT]^C
343 GTGCTC
BstEII 405 GGTCACC G^GT[ACGT]ACC
1393 GGTCACC
BstPI 405 GGTCACC G^GT[ACGT]ACC
1393 GGTCACC
CelII 25 GCTGAGC GC^T[ACGT]AGC
50 GCTTAGC
EcoO65I 405 GGTCACC G^GT[ACGT]ACC
1393 GGTCACC
HaeII 47 AGCGCT [GA]GCGC^[CT]
1613 GGCGCT
MaeI 31 CTAG C^TAG
220 CTAG
MluNI 528 TGGCCA TGG^CCA
1667 TGGCCA
MscI 528 TGGCCA TGG^CCA
1667 TGGCCA
PstI 319 CTGCAG CTGCA^G
352 CTGCAG
PvuII 349 CAGCTG CAG^CTG
1100 CAGCTG
TfiI 517 GAATC G^A[AT]TC
691 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AfaI AluI AvaII BanII
BbsI BbvI BmyI BpmI BpuAI
BsaJI BsaWI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI