(Circular) (Six-base) MAPSORT of: pFN21AB8374.seq Check: 3315 from: 1 to: 6589 pFN21AB8374 6589 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3309 4399 278 Size: 53 83 186 936 1773 1090 2468 Fragments arranged by size: 2468 1773 1090 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3345 3932 1937 Size: 1408 587 4594 Fragments arranged by size: 4594 1408 587 Acc65I G'GTAC_C Cuts at: 3910 3910 Size: 6589 AclI AA'CG_TT Cuts at: 1563 4716 5089 1563 Size: 3153 373 3063 Fragments arranged by size: 3153 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2511 2712 4652 6036 6304 1632 Size: 879 201 1940 1384 268 1917 Fragments arranged by size: 1940 1917 1384 879 268 201 AfeI AGC'GCT Cuts at: 2054 2054 Size: 6589 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6392 AflIII A'CryG_T Cuts at: 1203 5488 6465 1203 Size: 4285 977 1327 Fragments arranged by size: 4285 1327 977 AgeI A'CCGG_T Cuts at: 5420 5420 Size: 6589 AhdI GACnn_n'nnGTC Cuts at: 5318 5318 Size: 6589 AleI CACnn'nnGTG Cuts at: 1166 2417 1166 Size: 1251 5338 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6589 AlwNI CAG_nnn'CTG Cuts at: 3110 4332 5904 3110 Size: 1222 1572 3795 Fragments arranged by size: 3795 1572 1222 ApaI G_GGCC'C Cuts at: 3446 3715 3446 Size: 269 6320 ApaLI G'TGCA_C Cuts at: 4646 5802 4646 Size: 1156 5433 ApoI r'AATT_y Cuts at: 1493 2639 3834 3898 4124 4276 6385 1493 Size: 1146 1195 64 226 152 2109 1697 Fragments arranged by size: 2109 1697 1195 1146 226 152 64 AseI AT'TA_AT Cuts at: 160 1032 5143 160 Size: 872 4111 1606 Fragments arranged by size: 4111 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6589 AvaI C'yCGr_G Cuts at: 1870 1943 3037 3914 1870 Size: 73 1094 877 4545 Fragments arranged by size: 4545 1094 877 73 BamHI G'GATC_C Cuts at: 3919 4286 3919 Size: 367 6222 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3910 5365 618 Size: 325 217 627 2123 1455 1842 Fragments arranged by size: 2123 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2686 3446 3715 3908 1391 Size: 1295 760 269 193 4072 Fragments arranged by size: 4072 1295 760 269 193 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2389 2953 3546 928 Size: 971 490 564 593 3971 Fragments arranged by size: 3971 971 593 564 490 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4780 4814 1903 Size: 34 2843 34 3678 Fragments arranged by size: 3678 2843 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4481 5691 4481 Size: 1210 5379 BclI T'GATC_A Cuts at: 1548 1548 Size: 6589 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5200 136 Size: 107 122 71 631 605 3528 1525 Fragments arranged by size: 3528 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6584 6584 Size: 6589 BlpI GC'TnA_GC Cuts at: 2031 2056 4006 2031 Size: 25 1950 4614 Fragments arranged by size: 4614 1950 25 Bme1580I G_kGCm'C Cuts at: 1790 3446 3715 4650 5806 1790 Size: 1656 269 935 1156 2573 Fragments arranged by size: 2573 1656 1156 935 269 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6589 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2265 2405 4320 Size: 416 53 83 399 24 848 140 1915 Cuts at: 4320 5278 442 Size: 958 1753 Fragments arranged by size: 1915 1753 958 848 416 399 140 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6589 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2336 2781 2828 3066 3226 5249 1239 Size: 1097 445 47 238 160 2023 2579 Fragments arranged by size: 2579 2023 1097 445 238 160 47 Bpu10I CC'TnA_GC Cuts at: 2853 2853 Size: 6589 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4714 5579 5877 4714 Size: 865 298 5426 Fragments arranged by size: 5426 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2453 5252 882 Size: 617 954 2799 2219 Fragments arranged by size: 2799 2219 954 617 BsaAI yAC'GTr Cuts at: 493 1204 4380 493 Size: 711 3176 2702 Fragments arranged by size: 3176 2702 711 BsaBI GATnn'nnATC Cuts at: 2160 6583 2160 Size: 4423 2166 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3306 4396 4778 5413 Size: 53 83 186 936 1773 1090 382 635 Cuts at: 5413 275 Size: 1451 Fragments arranged by size: 1773 1451 1090 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2212 2658 3281 5022 5420 5694 5841 6252 2212 Size: 446 623 1741 398 274 147 411 2549 Fragments arranged by size: 2549 1741 623 446 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2452 2482 3236 3266 1178 Size: 30 165 30 1049 30 754 30 4501 Fragments arranged by size: 4501 1049 754 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2108 2239 2460 3875 1186 Size: 922 131 221 1415 3900 Fragments arranged by size: 3900 1415 922 221 131 BseYI C'CCAG_C Cuts at: 5792 6288 5792 Size: 496 6093 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3096 3107 1163 Size: 573 1360 11 4645 Fragments arranged by size: 4645 1360 573 11 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2725 4039 4800 5828 1540 Size: 275 186 724 1314 761 1028 2301 Fragments arranged by size: 2301 1314 1028 761 724 275 186 BsiHKAI G_wGCw'C Cuts at: 2267 2352 4650 4735 5806 2267 Size: 85 2298 85 1071 3050 Fragments arranged by size: 3050 2298 1071 85 85 BsmI GAATG_Cn' Cuts at: 3163 4100 4193 3163 Size: 937 93 5559 Fragments arranged by size: 5559 937 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3297 3297 Size: 6589 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2267 2352 2686 3446 3715 3908 4650 Size: 399 477 85 334 760 269 193 742 Cuts at: 4650 4735 5806 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 760 742 477 399 334 269 193 85 85 BspEI T'CCGG_A Cuts at: 6252 6252 Size: 6589 BspHI T'CATG_A Cuts at: 4478 6208 4478 Size: 1730 4859 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3365 3944 844 Size: 2521 579 3489 Fragments arranged by size: 3489 2521 579 BsrBI CCG'CTC Cuts at: 1394 1823 3526 4476 1394 Size: 429 1703 950 3507 Fragments arranged by size: 3507 1703 950 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5084 5258 65 Size: 1183 3836 174 1396 Fragments arranged by size: 3836 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5233 5420 1700 Size: 110 3423 187 2869 Fragments arranged by size: 3423 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3252 96 Size: 3156 3433 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6589 BssSI C'ACGA_G Cuts at: 4649 5661 4649 Size: 1012 5577 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6589 BstBI TT'CG_AA Cuts at: 5393 5393 Size: 6589 BstEII G'GTnAC_C Cuts at: 1184 2410 3398 1184 Size: 1226 988 4375 Fragments arranged by size: 4375 1226 988 BstYI r'GATC_y Cuts at: 1972 2622 3919 4286 4671 4688 6140 6226 6238 Size: 650 1297 367 385 17 1452 86 12 Cuts at: 6238 6584 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1297 650 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6037 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5850 Fragments arranged by size: 5850 683 56 BtsI GCAGTG_nn' Cuts at: 2333 3334 3444 4105 4899 4919 2333 Size: 1001 110 661 794 20 4003 Fragments arranged by size: 4003 1001 794 661 110 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6589 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6554 DraI TTT'AAA Cuts at: 3893 3893 Size: 6589 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3491 5596 809 Size: 507 2175 2105 1802 Fragments arranged by size: 2175 2105 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2533 2722 3672 Size: 54 1425 50 275 18 703 189 950 Cuts at: 3672 4036 4925 6554 8 Size: 364 889 1629 43 Fragments arranged by size: 1629 1425 950 889 703 364 275 189 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2722 4036 1537 Size: 275 910 1314 4090 Fragments arranged by size: 4090 1314 910 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2067 2085 2487 4519 1353 Size: 714 18 402 2032 3423 Fragments arranged by size: 3423 2032 714 402 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3679 5176 5550 5696 387 Size: 1064 138 2090 1497 374 146 1280 Fragments arranged by size: 2090 1497 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2336 2511 2712 2781 2828 3066 3226 Size: 393 704 175 201 69 47 238 160 Cuts at: 3226 4652 5249 6036 6304 1239 Size: 1426 597 787 268 1524 Fragments arranged by size: 1524 1426 787 704 597 393 268 238 201 175 160 69 47 EcoO109I rG'GnC_Cy Cuts at: 2319 3150 3822 2319 Size: 831 672 5086 Fragments arranged by size: 5086 831 672 EcoRI G'AATT_C Cuts at: 3898 4276 3898 Size: 378 6211 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2071 2071 Size: 6589 FspI TGC'GCA Cuts at: 5095 5095 Size: 6589 HaeII r_GCGC'y Cuts at: 1678 2056 3622 5736 1678 Size: 378 1566 2114 2531 Fragments arranged by size: 2531 2114 1566 378 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2452 2484 3206 3238 3671 Size: 32 135 32 880 32 722 32 433 Cuts at: 3671 3703 5230 5262 5304 5336 1373 Size: 32 1527 32 42 32 2626 Fragments arranged by size: 2626 1527 880 722 433 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2601 3933 4179 669 Size: 988 281 663 1332 246 3079 Fragments arranged by size: 3079 1332 988 663 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6589 HpaI GTT'AAC Cuts at: 4179 4179 Size: 6589 KpnI G_GTAC'C Cuts at: 3914 3914 Size: 6589 MfeI C'AATT_G Cuts at: 4188 4188 Size: 6589 MluI A'CGCG_T Cuts at: 6465 6465 Size: 6589 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2724 3683 3754 5703 5887 1305 Size: 1419 959 71 1949 184 2007 Fragments arranged by size: 2007 1949 1419 959 184 71 MscI TGG'CCA Cuts at: 10 64 1489 1832 2535 3674 10 Size: 54 1425 343 703 1139 2925 Fragments arranged by size: 2925 1425 1139 703 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2417 2828 3437 3798 4547 4906 5065 Size: 648 1251 411 609 361 749 359 159 Cuts at: 5065 518 Size: 2042 Fragments arranged by size: 2042 1251 749 648 609 411 361 359 159 MspA1I CmG'CkG Cuts at: 1606 2356 2721 3091 3107 4005 4682 5830 6075 Size: 750 365 370 16 898 677 1148 245 Cuts at: 6075 1606 Size: 2120 Fragments arranged by size: 2120 1148 898 750 677 370 365 245 16 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6037 NdeI CA'TA_TG Cuts at: 387 387 Size: 6589 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6589 NotI GC'GGCC_GC Cuts at: 4036 4036 Size: 6589 NspI r_CATG'y Cuts at: 1132 1229 3220 3947 5492 1132 Size: 97 1991 727 1545 2229 Fragments arranged by size: 2229 1991 1545 727 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6589 PciI A'CATG_T Cuts at: 5488 5488 Size: 6589 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6589 PfoI T'CCnGG_A Cuts at: 3203 3203 Size: 6589 PmeI GTTT'AAAC Cuts at: 3893 3893 Size: 6589 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4658 4690 6171 6203 4658 Size: 32 1481 32 5044 Fragments arranged by size: 5044 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2319 3150 3822 2319 Size: 831 672 5086 Fragments arranged by size: 5086 831 672 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6589 PsiI TTA'TAA Cuts at: 4159 4159 Size: 6589 PspOMI G'GGCC_C Cuts at: 3442 3711 3442 Size: 269 6320 PstI C_TGCA'G Cuts at: 830 2328 2361 3941 830 Size: 1498 33 1580 3478 Fragments arranged by size: 3478 1580 1498 33 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6589 PvuII CAG'CTG Cuts at: 2356 3107 2356 Size: 751 5838 SalI G'TCGA_C Cuts at: 1936 3931 1936 Size: 1995 4594 SanDI GG'GwC_CC Cuts at: 3150 3150 Size: 6589 SbfI CC_TGCA'GG Cuts at: 3941 3941 Size: 6589 ScaI AGT'ACT Cuts at: 4837 6549 4837 Size: 1712 4877 SfcI C'TryA_G Cuts at: 826 1045 2324 2357 2716 3937 5072 5753 5944 Size: 219 1279 33 359 1221 1135 681 191 Cuts at: 5944 826 Size: 1471 Fragments arranged by size: 1471 1279 1221 1135 681 359 219 191 33 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6589 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6589 SmaI CCC'GGG Cuts at: 1872 3916 1872 Size: 2044 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4693 5594 5856 820 Size: 197 926 2750 901 262 1553 Fragments arranged by size: 2750 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4380 493 Size: 3887 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6589 SphI G_CATG'C Cuts at: 1132 3947 1132 Size: 2815 3774 SspI AAT'ATT Cuts at: 5 52 4513 5 Size: 47 4461 2081 Fragments arranged by size: 4461 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6589 StyI C'CwwG_G Cuts at: 513 1065 2432 2916 3403 4028 513 Size: 552 1367 484 487 625 3074 Fragments arranged by size: 3074 1367 625 552 487 484 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2429 2977 4616 4633 4786 4971 5416 Size: 1255 849 548 1639 17 153 185 445 Cuts at: 5416 325 Size: 1498 Fragments arranged by size: 1639 1498 1255 849 548 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3129 3252 4835 Size: 274 80 33 51 1443 1152 123 1583 Cuts at: 4835 6547 96 Size: 1712 138 Fragments arranged by size: 1712 1583 1443 1152 274 138 123 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2957 2989 3640 3672 6549 6581 2957 Size: 32 651 32 2877 32 2965 Fragments arranged by size: 2965 2877 651 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6589 XbaI T'CTAG_A Cuts at: 3925 3925 Size: 6589 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2815 2903 3826 2815 Size: 88 923 5578 Fragments arranged by size: 5578 923 88 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6589 XmaI C'CCGG_G Cuts at: 1870 3914 1870 Size: 2044 4545 XmnI GAAnn'nnTTC Cuts at: 2139 4718 2139 Size: 2579 4010 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3307 4397 276 Size: 53 83 186 936 1773 1090 2468 Fragments arranged by size: 2468 1773 1090 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI