Restriction map for
pFN21AB8363
##################################
# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AgeI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I AvrII BalI BamHI BbeI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmI Bsp1407I BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstUI BstZ17I Bsu36I CelII Cfr10I
CpoI DraI DraIII DrdI EaeI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRV FauI FbaI FseI
HaeII Hin1I HindIII HpaI KasI
KspI MaeI MfeI MluI MluNI
MroI MscI MspA1I MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
StuI SwaI XbaI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 1094 GGTACC G^GTACC
AciI 1092 GCGG CCGC(-3/-1)
AflIII 557 ACGTGT A^C[GA][CT]GT
AhdI 925 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1297 GGGCCC GGGCC^C
Asp718I 1094 GGTACC G^GTACC
AspEI 925 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 415 GAGCTC G[AT]GC[AT]^C
AspI 1111 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1210 CTCGAG C^[CT]CG[GA]G
AviII 1121 TGCGCA TGC^GCA
BanI 1094 GGTACC G^G[CT][GA]CC
BbrPI 1375 CACGTG CAC^GTG
BbsI 1380 GAAGAC GAAGAC(2/6)
BpmI 269 CTGGAG CTGGAG(16/14)
BpuAI 1380 GAAGAC GAAGAC(2/6)
BsaAI 1375 CACGTG [CT]AC^GT[GA]
BsaBI 201 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1310 GTGCAG GTGCAG(16/14)
BsiHKAI 415 GAGCTC G[AT]GC[AT]^C
BsmBI 1365 GAGACG CGTCTC(1/5)
BsmFI 1171 GGGAC GGGAC(10/14)
BsoBI 1210 CTCGAG C^[CT]CG[GA]G
BspDI 1147 ATCGAT AT^CGAT
BstAPI 212 GCAGTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 412 CCAGAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 1122 GCGC GCG^C
ClaI 1147 ATCGAT AT^CGAT
Eam1105I 925 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 247 GAATTC G^AATTC
FspI 1121 TGCGCA TGC^GCA
HhaI 1122 GCGC GCG^C
HinP1I 1122 GCGC G^CGC
HincII 561 GTTGAC GT[CT]^[GA]AC
HindII 561 GTTGAC GT[CT]^[GA]AC
KpnI 1094 GGTACC GGTAC^C
MamI 201 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 1328 CCATGG C^CATGG
PaeR7I 1210 CTCGAG C^TCGAG
PflFI 1111 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 142 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1375 CACGTG CAC^GTG
PmlI 1375 CACGTG CAC^GTG
PpuMI 144 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1492 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 364 CTGCAG CTGCA^G
SacI 415 GAGCTC GAGCT^C
SapI 603 GCTCTTC GCTCTTC(1/4)
SexAI 141 ACCAGGT A^CC[AT]GGT
SfcI 364 CTGCAG C^T[GA][CT]AG
SphI 1088 GCATGC GCATG^C
SspI 1189 AATATT AAT^ATT
Tth111I 1111 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 142 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1008 CCAGACTGTTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1210 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AvaII 145 GGTCC G^G[AT]CC
169 GGACC
BanII 415 GAGCTC G[GA]GC[CT]^C
1297 GGGCCC
BcnI 234 CCGGG CC^[GC]GG
463 CCCGG
BsiYI 142 CCAGGTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
195 CCTGTGGATGG
BslI 142 CCAGGTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
195 CCTGTGGATGG
BsrDI 963 CATTGC GCAATG(2/0)
1385 CATTGC
BstEII 531 GGTGACC G^GT[ACGT]ACC
865 GGTGACC
BstNI 142 CCAGG CC^[AT]GG
148 CCTGG
BstPI 531 GGTGACC G^GT[ACGT]ACC
865 GGTGACC
BstYI 263 GGATCT [GA]^GATC[CT]
614 GGATCT
EcoO65I 531 GGTGACC G^GT[ACGT]ACC
865 GGTGACC
EcoRII 142 CCAGG ^CC[AT]GG
148 CCTGG
EcoT14I 651 CCAAGG C^C[AT][AT]GG
1328 CCATGG
EcoT22I 379 ATGCAT ATGCA^T
665 ATGCAT
HapII 234 CCGG C^CGG
464 CCGG
HgaI 788 GACGC GACGC(5/10)
573 GCGTC
HpaII 234 CCGG C^CGG
464 CCGG
MflI 263 GGATCT [GA]^GATC[CT]
614 GGATCT
MspI 234 CCGG C^CGG
464 CCGG
MvaI 142 CCAGG CC^[AT]GG
148 CCTGG
NciI 234 CCGGG CC^[GC]GG
463 CCCGG
NsiI 379 ATGCAT ATGCA^T
665 ATGCAT
NspI 1088 GCATGC [GA]CATG^[CT]
1457 ACATGC
StyI 651 CCAAGG C^C[AT][AT]GG
1328 CCATGG
XhoII 263 GGATCT [GA]^GATC[CT]
614 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI BbvI BmyI BsaJI BseRI
BsmAI Bsp1286I BsrI BssKI BstF5I
Cac8I Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MseI MslI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I