(Circular) (Six-base) MAPSORT of: pFN21AB8362.seq Check: 3059 from: 1 to: 6034 pFN21AB8362 6034 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2366 2487 2951 3844 Size: 53 83 186 936 830 121 464 893 Cuts at: 3844 278 Size: 2468 Fragments arranged by size: 2468 936 893 830 464 186 121 83 53 AccI GT'mk_AC Cuts at: 1937 2400 2645 3377 1937 Size: 463 245 732 4594 Fragments arranged by size: 4594 732 463 245 Acc65I G'GTAC_C Cuts at: 2226 3355 2226 Size: 1129 4905 AclI AA'CG_TT Cuts at: 1563 4161 4534 1563 Size: 2598 373 3063 Fragments arranged by size: 3063 2598 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3214 4097 5481 5749 1632 Size: 1582 883 1384 268 1917 Fragments arranged by size: 1917 1582 1384 883 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5837 AflIII A'CryG_T Cuts at: 1203 3201 4933 5910 1203 Size: 1998 1732 977 1327 Fragments arranged by size: 1998 1732 1327 977 AgeI A'CCGG_T Cuts at: 4865 4865 Size: 6034 AhdI GACnn_n'nnGTC Cuts at: 4763 4763 Size: 6034 AleI CACnn'nnGTG Cuts at: 1166 2607 1166 Size: 1441 4593 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3152 1184 Size: 1968 4066 AlwNI CAG_nnn'CTG Cuts at: 3777 5349 3777 Size: 1572 4462 ApaI G_GGCC'C Cuts at: 2391 2391 Size: 6034 ApaLI G'TGCA_C Cuts at: 4091 5247 4091 Size: 1156 4878 ApoI r'AATT_y Cuts at: 1493 3343 3569 3721 5830 1493 Size: 1850 226 152 2109 1697 Fragments arranged by size: 2109 1850 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4588 160 Size: 872 3556 1606 Fragments arranged by size: 3556 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6034 AvaI C'yCGr_G Cuts at: 1870 1943 2089 2383 2684 3359 1870 Size: 73 146 294 301 675 4545 Fragments arranged by size: 4545 675 301 294 146 73 BamHI G'GATC_C Cuts at: 2279 3364 3731 2279 Size: 1085 367 4582 Fragments arranged by size: 4582 1085 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2226 2566 3355 4810 618 Size: 325 217 627 439 340 789 1455 1842 Fragments arranged by size: 1842 1455 789 627 439 340 325 217 BanII G_rGCy'C Cuts at: 1391 2391 2871 3033 3353 1391 Size: 1000 480 162 320 4072 Fragments arranged by size: 4072 1000 480 320 162 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5063 BbvCI CC'TCA_GC Cuts at: 2096 2096 Size: 6034 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4225 4259 1903 Size: 34 2288 34 3678 Fragments arranged by size: 3678 2288 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3926 5136 3926 Size: 1210 4824 BclI T'GATC_A Cuts at: 1548 1548 Size: 6034 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2528 2724 4645 Size: 107 122 71 631 605 856 196 1921 Cuts at: 4645 136 Size: 1525 Fragments arranged by size: 1921 1525 856 631 605 196 122 107 71 BglII A'GATC_T Cuts at: 6029 6029 Size: 6034 BlpI GC'TnA_GC Cuts at: 3451 3451 Size: 6034 Bme1580I G_kGCm'C Cuts at: 1790 2391 2571 2685 4095 5251 1790 Size: 601 180 114 1410 1156 2573 Fragments arranged by size: 2573 1410 1156 601 180 114 BmgBI CAC'GTC Cuts at: 1318 2182 1318 Size: 864 5170 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3765 4723 442 Size: 416 53 83 399 24 2348 958 1753 Fragments arranged by size: 2348 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6034 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2247 2710 2247 Size: 463 5571 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2612 3007 3170 3178 3223 3295 4694 1239 Size: 1373 395 163 8 45 72 1399 2579 Fragments arranged by size: 2579 1399 1373 395 163 72 45 8 Bpu10I CC'TnA_GC Cuts at: 2096 3212 2096 Size: 1116 4918 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2540 4159 5024 5322 2540 Size: 1619 865 298 3252 Fragments arranged by size: 3252 1619 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4697 882 Size: 617 3198 2219 Fragments arranged by size: 3198 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2680 3825 493 Size: 711 1476 1145 2702 Fragments arranged by size: 2702 1476 1145 711 BsaBI GATnn'nnATC Cuts at: 6028 6028 Size: 6034 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2363 2484 2948 3841 Size: 53 83 186 936 830 121 464 893 Cuts at: 3841 4223 4858 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 893 830 635 464 382 186 121 83 53 BsaWI w'CCGG_w Cuts at: 2696 4467 4865 5139 5286 5697 2696 Size: 1771 398 274 147 411 3033 Fragments arranged by size: 3033 1771 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2315 2345 2363 2393 3191 Size: 30 165 30 912 30 18 30 798 Cuts at: 3191 3221 3293 3323 1178 Size: 30 72 30 3889 Fragments arranged by size: 3889 912 798 165 72 30 30 30 30 30 30 18 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2153 2410 2629 3004 1186 Size: 967 257 219 375 4216 Fragments arranged by size: 4216 967 375 257 219 BseYI C'CCAG_C Cuts at: 2031 2711 2722 5237 5733 2031 Size: 680 11 2515 496 2332 Fragments arranged by size: 2515 2332 680 496 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2240 1163 Size: 573 504 4957 Fragments arranged by size: 4957 573 504 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2523 3186 3484 4245 5273 1540 Size: 275 186 522 663 298 761 1028 2301 Fragments arranged by size: 2301 1028 761 663 522 298 275 186 BsiHKAI G_wGCw'C Cuts at: 2061 4095 4180 5251 2061 Size: 2034 85 1071 2844 Fragments arranged by size: 2844 2034 1071 85 BsmI GAATG_Cn' Cuts at: 3001 3545 3638 3001 Size: 544 93 5397 Fragments arranged by size: 5397 544 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2491 2491 Size: 6034 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2061 2391 2571 2685 2871 3033 3353 Size: 399 271 330 180 114 186 162 320 Cuts at: 3353 4095 4180 5251 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 330 320 271 186 180 162 114 85 BspEI T'CCGG_A Cuts at: 2696 5697 2696 Size: 3001 3033 BspHI T'CATG_A Cuts at: 3923 5653 3923 Size: 1730 4304 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3389 844 Size: 2545 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3921 1394 Size: 429 2098 3507 Fragments arranged by size: 3507 2098 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3025 4529 4703 65 Size: 1183 1777 1504 174 1396 Fragments arranged by size: 1777 1504 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2522 2738 2895 4678 4865 1700 Size: 110 712 216 157 1783 187 2869 Fragments arranged by size: 2869 1783 712 216 187 157 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6034 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6034 BssSI C'ACGA_G Cuts at: 4094 5106 4094 Size: 1012 5022 BstAPI GCAn_nnn'nTGC Cuts at: 794 2862 794 Size: 2068 3966 BstBI TT'CG_AA Cuts at: 4838 4838 Size: 6034 BstEII G'GTnAC_C Cuts at: 1184 3319 1184 Size: 2135 3899 BstXI CCAn_nnnn'nTGG Cuts at: 2609 2609 Size: 6034 BstYI r'GATC_y Cuts at: 1972 2279 3364 3731 4116 4133 5585 5671 5683 Size: 307 1085 367 385 17 1452 86 12 Cuts at: 5683 6029 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1085 385 367 346 307 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2214 2242 2374 2608 3327 513 Size: 552 939 210 28 132 234 719 3220 Fragments arranged by size: 3220 939 719 552 234 210 132 28 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2484 487 Size: 683 56 1258 4037 Fragments arranged by size: 4037 1258 683 56 BtsI GCAGTG_nn' Cuts at: 3550 4344 4364 3550 Size: 794 20 5220 Fragments arranged by size: 5220 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6034 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3007 3042 529 Size: 35 2443 35 3521 Fragments arranged by size: 3521 2443 35 35 DraI TTT'AAA Cuts at: 3338 3338 Size: 6034 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5041 809 Size: 507 3725 1802 Fragments arranged by size: 3725 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2520 3183 3481 Size: 54 1425 50 275 18 690 663 298 Cuts at: 3481 4370 5999 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 690 663 298 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2520 3183 3481 1537 Size: 275 708 663 298 4090 Fragments arranged by size: 4090 708 663 298 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2459 2708 3964 1353 Size: 1106 249 1256 3423 Fragments arranged by size: 3423 1256 1106 249 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2033 4621 4995 5141 387 Size: 1064 138 444 2588 374 146 1280 Fragments arranged by size: 2588 1280 1064 444 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2612 3007 3170 3178 3214 3223 3295 Size: 393 980 395 163 8 36 9 72 Cuts at: 3295 4097 4694 5481 5749 1239 Size: 802 597 787 268 1524 Fragments arranged by size: 1524 980 802 787 597 395 393 268 163 72 36 9 8 EcoO109I rG'GnC_Cy Cuts at: 2387 2388 3007 2387 Size: 1 619 5414 Fragments arranged by size: 5414 619 1 EcoRI G'AATT_C Cuts at: 3343 3721 3343 Size: 378 5656 FspI TGC'GCA Cuts at: 4540 4540 Size: 6034 HaeII r_GCGC'y Cuts at: 1678 5181 1678 Size: 3503 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4675 4707 4749 4781 1373 Size: 32 135 32 3103 32 42 32 2626 Fragments arranged by size: 3103 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2646 3378 3624 669 Size: 988 281 708 732 246 3079 Fragments arranged by size: 3079 988 732 708 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6034 HpaI GTT'AAC Cuts at: 3624 3624 Size: 6034 KpnI G_GTAC'C Cuts at: 2230 3359 2230 Size: 1129 4905 MfeI C'AATT_G Cuts at: 3633 3633 Size: 6034 MluI A'CGCG_T Cuts at: 5910 5910 Size: 6034 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2258 5148 5332 1305 Size: 953 2890 184 2007 Fragments arranged by size: 2890 2007 953 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4212 Fragments arranged by size: 4212 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2607 3266 3326 3992 4351 4510 Size: 648 843 598 659 60 666 359 159 Cuts at: 4510 518 Size: 2042 Fragments arranged by size: 2042 843 666 659 648 598 359 159 60 MspA1I CmG'CkG Cuts at: 1606 2131 3450 4127 5275 5520 1606 Size: 525 1319 677 1148 245 2120 Fragments arranged by size: 2120 1319 1148 677 525 245 NaeI GCC'GGC Cuts at: 2524 2524 Size: 6034 NcoI C'CATG_G Cuts at: 513 1065 2004 3327 513 Size: 552 939 1323 3220 Fragments arranged by size: 3220 1323 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6034 NgoMIV G'CCGG_C Cuts at: 2522 2522 Size: 6034 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6034 NotI GC'GGCC_GC Cuts at: 3481 3481 Size: 6034 NspI r_CATG'y Cuts at: 1132 1229 3205 3250 3392 4937 1132 Size: 97 1976 45 142 1545 2229 Fragments arranged by size: 2229 1976 1545 142 97 45 PasI CC'CwG_GG Cuts at: 1348 2311 2626 1348 Size: 963 315 4756 Fragments arranged by size: 4756 963 315 PciI A'CATG_T Cuts at: 3201 4933 3201 Size: 1732 4302 PflMI CCAn_nnn'nTGG Cuts at: 1108 2985 1108 Size: 1877 4157 PmeI GTTT'AAAC Cuts at: 3338 3338 Size: 6034 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4103 4135 5616 5648 4103 Size: 32 1481 32 4489 Fragments arranged by size: 4489 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3007 3007 Size: 6034 PshAI GACnn'nnGTC Cuts at: 1613 3202 1613 Size: 1589 4445 PsiI TTA'TAA Cuts at: 3604 3604 Size: 6034 PspOMI G'GGCC_C Cuts at: 2387 2387 Size: 6034 PstI C_TGCA'G Cuts at: 830 2932 3386 830 Size: 2102 454 3478 Fragments arranged by size: 3478 2102 454 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6034 SalI G'TCGA_C Cuts at: 1936 2644 3376 1936 Size: 708 732 4594 Fragments arranged by size: 4594 732 708 SanDI GG'GwC_CC Cuts at: 3007 3007 Size: 6034 SbfI CC_TGCA'GG Cuts at: 3386 3386 Size: 6034 ScaI AGT'ACT Cuts at: 4282 5994 4282 Size: 1712 4322 SexAI A'CCwGG_T Cuts at: 2562 2562 Size: 6034 SfcI C'TryA_G Cuts at: 826 1045 2928 3382 4517 5198 5389 826 Size: 219 1883 454 1135 681 191 1471 Fragments arranged by size: 1883 1471 1135 681 454 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2528 2528 Size: 6034 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6034 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6034 SmaI CCC'GGG Cuts at: 1872 2091 2385 3361 1872 Size: 219 294 976 4545 Fragments arranged by size: 4545 976 294 219 SmlI C'TyrA_G Cuts at: 820 1017 1943 2555 4138 5039 5301 820 Size: 197 926 612 1583 901 262 1553 Fragments arranged by size: 1583 1553 926 901 612 262 197 SnaBI TAC'GTA Cuts at: 493 3825 493 Size: 3332 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6034 SphI G_CATG'C Cuts at: 1132 3392 1132 Size: 2260 3774 SspI AAT'ATT Cuts at: 5 52 3052 3958 5 Size: 47 3000 906 2081 Fragments arranged by size: 3000 2081 906 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6034 StyI C'CwwG_G Cuts at: 513 1065 2004 2763 3327 3473 513 Size: 552 939 759 564 146 3074 Fragments arranged by size: 3074 939 759 564 552 146 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2023 2194 2618 3320 4061 4078 4231 Size: 1255 443 171 424 702 741 17 153 Cuts at: 4231 4416 4861 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 741 702 445 443 424 185 171 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2688 4280 5992 Size: 274 80 33 51 1443 711 1592 1712 Cuts at: 5992 96 Size: 138 Fragments arranged by size: 1712 1592 1443 711 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2315 2347 2363 2395 2938 2970 3191 3223 3263 Size: 32 16 32 543 32 221 32 40 Cuts at: 3263 3293 3295 3325 5994 6026 2315 Size: 30 2 30 2669 32 2323 Fragments arranged by size: 2669 2323 543 221 40 32 32 32 32 32 30 30 16 2 Tth111I GACn'n_nGTC Cuts at: 1316 3011 1316 Size: 1695 4339 XbaI T'CTAG_A Cuts at: 3370 3370 Size: 6034 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2719 2719 Size: 6034 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6034 XmaI C'CCGG_G Cuts at: 1870 2089 2383 3359 1870 Size: 219 294 976 4545 Fragments arranged by size: 4545 976 294 219 XmnI GAAnn'nnTTC Cuts at: 4163 4163 Size: 6034 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2364 2485 2949 3842 Size: 53 83 186 936 830 121 464 893 Cuts at: 3842 276 Size: 2468 Fragments arranged by size: 2468 936 893 830 464 186 121 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SapI SfoI SrfI SwaI