Restriction map for
pFN21AB8358
(Circular) (Six-base) MAPSORT of: pFN21AB8358.seq Check: 767 from: 1 to: 5737
pFN21AB8358 5737 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3547 278
Size: 53 83 186 936 2011 2468
Fragments arranged by size:
2468 2011 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2241 3080 1937
Size: 304 839 4594
Fragments arranged by size:
4594 839 304
Acc65I G'GTAC_C
Cuts at: 3058 3058
Size: 5737
AclI AA'CG_TT
Cuts at: 1563 3864 4237 1563
Size: 2301 373 3063
Fragments arranged by size:
3063 2301 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2630 3800 5184 5452 1632
Size: 998 1170 1384 268 1917
Fragments arranged by size:
1917 1384 1170 998 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5540
AflIII A'CryG_T
Cuts at: 1203 4636 5613 1203
Size: 3433 977 1327
Fragments arranged by size:
3433 1327 977
AgeI A'CCGG_T
Cuts at: 4568 4568
Size: 5737
AhdI GACnn_n'nnGTC
Cuts at: 4466 4466
Size: 5737
AleI CACnn'nnGTG
Cuts at: 1166 2096 1166
Size: 930 4807
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5737
AlwNI CAG_nnn'CTG
Cuts at: 3480 5052 3480
Size: 1572 4165
ApaI G_GGCC'C
Cuts at: 2453 2453
Size: 5737
ApaLI G'TGCA_C
Cuts at: 3794 4950 3794
Size: 1156 4581
ApoI r'AATT_y
Cuts at: 1493 2411 3046 3272 3424 5533 1493
Size: 918 635 226 152 2109 1697
Fragments arranged by size:
2109 1697 918 635 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4291 160
Size: 872 3259 1606
Fragments arranged by size:
3259 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5737
AvaI C'yCGr_G
Cuts at: 1870 1943 3062 1870
Size: 73 1119 4545
Fragments arranged by size:
4545 1119 73
BamHI G'GATC_C
Cuts at: 3067 3434 3067
Size: 367 5370
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2423 2725 2744 3058 4513
Size: 325 217 627 636 302 19 314 1455
Cuts at: 4513 618
Size: 1842
Fragments arranged by size:
1842 1455 636 627 325 314 302 217
19
BanII G_rGCy'C
Cuts at: 1391 2377 2453 3056 1391
Size: 986 76 603 4072
Fragments arranged by size:
4072 986 603 76
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4766
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3928 3962 1903
Size: 34 50 34 1907 34 3678
Fragments arranged by size:
3678 1907 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3629 4839 3629
Size: 1210 4527
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5737
BfrBI ATG'CAT
Cuts at: 2008 2530 2008
Size: 522 5215
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2927 4348 136
Size: 107 122 71 631 605 1255 1421 1525
Fragments arranged by size:
1525 1421 1255 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5732 5732
Size: 5737
BlpI GC'TnA_GC
Cuts at: 2204 3154 2204
Size: 950 4787
Bme1580I G_kGCm'C
Cuts at: 1790 2062 2453 2568 2730 2747 3027 3798 4954
Size: 272 391 115 162 17 280 771 1156
Cuts at: 4954 1790
Size: 2573
Fragments arranged by size:
2573 1156 771 391 280 272 162 115
17
BmgBI CAC'GTC
Cuts at: 1318 2176 1318
Size: 858 4879
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2859 3468 4426
Size: 416 53 83 399 24 1442 609 958
Cuts at: 4426 442
Size: 1753
Fragments arranged by size:
1753 1442 958 609 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5737
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2165 2477 2865 4397 1239
Size: 926 312 388 1532 2579
Fragments arranged by size:
2579 1532 926 388 312
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2663 3862 4727 5025 2663
Size: 1199 865 298 3375
Fragments arranged by size:
3375 1199 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2141 4400 882
Size: 617 642 2259 2219
Fragments arranged by size:
2259 2219 642 617
BsaAI yAC'GTr
Cuts at: 493 1204 3528 493
Size: 711 2324 2702
Fragments arranged by size:
2702 2324 711
BsaBI GATnn'nnATC
Cuts at: 2027 5731 2027
Size: 3704 2033
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2085 2762 3544 3926
Size: 53 83 186 936 552 677 782 382
Cuts at: 3926 4561 275
Size: 635 1451
Fragments arranged by size:
1451 936 782 677 635 552 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4170 4568 4842 4989 5400 4170
Size: 398 274 147 411 4507
Fragments arranged by size:
4507 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2576 2606 2882 2912 1178
Size: 30 165 30 1173 30 276 30 4003
Fragments arranged by size:
4003 1173 276 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2812 2815 1186
Size: 1626 3 4108
Fragments arranged by size:
4108 1626 3
BseYI C'CCAG_C
Cuts at: 2509 2567 2795 2834 2839 4940 5436 2509
Size: 58 228 39 5 2101 496 2810
Fragments arranged by size:
2810 2101 496 228 58 39 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2198 1163
Size: 573 462 4702
Fragments arranged by size:
4702 573 462
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2198 3187 3948 4976 1540
Size: 275 186 197 989 761 1028 2301
Fragments arranged by size:
2301 1028 989 761 275 197 186
BsiHKAI G_wGCw'C
Cuts at: 3798 3883 4954 3798
Size: 85 1071 4581
Fragments arranged by size:
4581 1071 85
BsmI GAATG_Cn'
Cuts at: 2110 3248 3341 2110
Size: 1138 93 4506
Fragments arranged by size:
4506 1138 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2062 2377 2453 2568 2730 2747 3027
Size: 399 272 315 76 115 162 17 280
Cuts at: 3027 3056 3798 3883 4954 1391
Size: 29 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 315 280 272 162
115 85 76 29 17
BspEI T'CCGG_A
Cuts at: 5400 5400
Size: 5737
BspHI T'CATG_A
Cuts at: 3626 5356 3626
Size: 1730 4007
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2135 2170 3092 844
Size: 1291 35 922 3489
Fragments arranged by size:
3489 1291 922 35
BsrBI CCG'CTC
Cuts at: 1394 1823 3624 1394
Size: 429 1801 3507
Fragments arranged by size:
3507 1801 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4232 4406 65
Size: 1183 2984 174 1396
Fragments arranged by size:
2984 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4381 4568 1700
Size: 110 2571 187 2869
Fragments arranged by size:
2869 2571 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5737
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5737
BssSI C'ACGA_G
Cuts at: 3797 4809 3797
Size: 1012 4725
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2127 794
Size: 1333 4404
BstBI TT'CG_AA
Cuts at: 4541 4541
Size: 5737
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5737
BstXI CCAn_nnnn'nTGG
Cuts at: 2098 2098
Size: 5737
BstYI r'GATC_y
Cuts at: 1972 3067 3434 3819 3836 5288 5374 5386 5732
Size: 1095 367 385 17 1452 86 12 346
Cuts at: 5732 1972
Size: 1977
Fragments arranged by size:
1977 1452 1095 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2344 2721 2854 2872 513
Size: 552 1279 377 133 18 3378
Fragments arranged by size:
3378 1279 552 377 133 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2518 2620 487
Size: 683 56 1292 102 3604
Fragments arranged by size:
3604 1292 683 102 56
BtsI GCAGTG_nn'
Cuts at: 3253 4047 4067 3253
Size: 794 20 4923
Fragments arranged by size:
4923 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5737
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5702
DraI TTT'AAA
Cuts at: 2266 3041 2266
Size: 775 4962
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2180 4744 809
Size: 507 864 2564 1802
Fragments arranged by size:
2564 1802 864 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2195 3184 4073
Size: 54 1425 50 275 18 365 989 889
Cuts at: 4073 5702 8
Size: 1629 43
Fragments arranged by size:
1629 1425 989 889 365 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2195 3184 1537
Size: 275 383 989 4090
Fragments arranged by size:
4090 989 383 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3667 1353
Size: 2314 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2674 4324 4698 4844 387
Size: 1064 138 1085 1650 374 146 1280
Fragments arranged by size:
1650 1280 1085 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2165 2477 2630 2865 3800 4397 5184
Size: 393 533 312 153 235 935 597 787
Cuts at: 5184 5452 1239
Size: 268 1524
Fragments arranged by size:
1524 935 787 597 533 393 312 268
235 153
EcoNI CCTnn'n_nnAGG
Cuts at: 2315 2315
Size: 5737
EcoO109I rG'GnC_Cy
Cuts at: 2320 2449 2450 2320
Size: 129 1 5607
Fragments arranged by size:
5607 129 1
EcoRI G'AATT_C
Cuts at: 2411 3046 3424 2411
Size: 635 378 4724
Fragments arranged by size:
4724 635 378
FspI TGC'GCA
Cuts at: 4243 4243
Size: 5737
HaeII r_GCGC'y
Cuts at: 1678 3018 4884 1678
Size: 1340 1866 2531
Fragments arranged by size:
2531 1866 1340
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2759 2791 4378 4410 4452
Size: 32 135 32 1187 32 1587 32 42
Cuts at: 4452 4484 1373
Size: 32 2626
Fragments arranged by size:
2626 1587 1187 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3010 3081 3327 669
Size: 988 281 1072 71 246 3079
Fragments arranged by size:
3079 1072 988 281 246 71
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5737
HpaI GTT'AAC
Cuts at: 3327 3327
Size: 5737
KpnI G_GTAC'C
Cuts at: 3062 3062
Size: 5737
MfeI C'AATT_G
Cuts at: 3336 3336
Size: 5737
MluI A'CGCG_T
Cuts at: 5613 5613
Size: 5737
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4851 5035 1305
Size: 3546 184 2007
Fragments arranged by size:
3546 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3915
Fragments arranged by size:
3915 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2096 2627 3695 4054 4213 518
Size: 648 930 531 1068 359 159 2042
Fragments arranged by size:
2042 1068 930 648 531 359 159
MspA1I CmG'CkG
Cuts at: 1606 2200 2346 2757 2939 3153 3830 4978 5223
Size: 594 146 411 182 214 677 1148 245
Cuts at: 5223 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 594 411 245 214 182
146
NcoI C'CATG_G
Cuts at: 513 1065 2854 2872 513
Size: 552 1789 18 3378
Fragments arranged by size:
3378 1789 552 18
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5737
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5737
NotI GC'GGCC_GC
Cuts at: 3184 3184
Size: 5737
NsiI A_TGCA'T
Cuts at: 2010 2532 2010
Size: 522 5215
NspI r_CATG'y
Cuts at: 1132 1229 3095 4640 1132
Size: 97 1866 1545 2229
Fragments arranged by size:
2229 1866 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2255 1348
Size: 907 4830
PciI A'CATG_T
Cuts at: 4636 4636
Size: 5737
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2855 1108
Size: 1747 3990
PmeI GTTT'AAAC
Cuts at: 3041 3041
Size: 5737
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3806 3838 5319 5351 3806
Size: 32 1481 32 4192
Fragments arranged by size:
4192 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2320 2320
Size: 5737
PshAI GACnn'nnGTC
Cuts at: 1613 2318 1613
Size: 705 5032
PsiI TTA'TAA
Cuts at: 3307 3307
Size: 5737
PspOMI G'GGCC_C
Cuts at: 2449 2449
Size: 5737
PstI C_TGCA'G
Cuts at: 830 2970 3089 830
Size: 2140 119 3478
Fragments arranged by size:
3478 2140 119
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5737
PvuII CAG'CTG
Cuts at: 2939 2939
Size: 5737
SacII CC_GC'GG
Cuts at: 2347 2347
Size: 5737
SalI G'TCGA_C
Cuts at: 1936 3079 1936
Size: 1143 4594
SbfI CC_TGCA'GG
Cuts at: 3089 3089
Size: 5737
ScaI AGT'ACT
Cuts at: 3985 5697 3985
Size: 1712 4025
SfcI C'TryA_G
Cuts at: 826 1045 2966 3085 4220 4901 5092 826
Size: 219 1921 119 1135 681 191 1471
Fragments arranged by size:
1921 1471 1135 681 219 191 119
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5737
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5737
SmaI CCC'GGG
Cuts at: 1872 3064 1872
Size: 1192 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2678 3841 4742 5004 820
Size: 197 926 735 1163 901 262 1553
Fragments arranged by size:
1553 1163 926 901 735 262 197
SnaBI TAC'GTA
Cuts at: 493 3528 493
Size: 3035 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5737
SphI G_CATG'C
Cuts at: 1132 3095 1132
Size: 1963 3774
SspI AAT'ATT
Cuts at: 5 52 3661 5
Size: 47 3609 2081
Fragments arranged by size:
3609 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5737
StyI C'CwwG_G
Cuts at: 513 1065 2854 2872 3176 513
Size: 552 1789 18 304 3074
Fragments arranged by size:
3074 1789 552 304 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2903 3764 3781 3934 4119 4564 325
Size: 1255 1323 861 17 153 185 445 1498
Fragments arranged by size:
1498 1323 1255 861 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2574 3983 5695
Size: 274 80 33 51 1443 597 1409 1712
Cuts at: 5695 96
Size: 138
Fragments arranged by size:
1712 1443 1409 597 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2882 2914 5697 5729 2882
Size: 32 2783 32 2890
Fragments arranged by size:
2890 2783 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2237 1316
Size: 921 4816
XbaI T'CTAG_A
Cuts at: 3073 3073
Size: 5737
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2095 2095
Size: 5737
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5737
XmaI C'CCGG_G
Cuts at: 1870 3062 1870
Size: 1192 4545
XmnI GAAnn'nnTTC
Cuts at: 3866 3866
Size: 5737
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3545 276
Size: 53 83 186 936 2011 2468
Fragments arranged by size:
2468 2011 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PspXI PsrI RsrII SacI
SanDI SapI SexAI SfiI SfoI SrfI SwaI