Restriction map for
pFN21AB8358
(Linear) (Six-base) MAPSORT of: KIBB8358.seq Check: 6638 from: 1 to: 1032
KIBB8358.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AccI GT'mk_AC
Cuts at: 0 236 1032
Size: 236 796
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 1032
Size: 625 407
AleI CACnn'nnGTG
Cuts at: 0 91 1032
Size: 91 941
ApaI G_GGCC'C
Cuts at: 0 448 1032
Size: 448 584
ApoI r'AATT_y
Cuts at: 0 406 1032
Size: 406 626
BanI G'GyrC_C
Cuts at: 0 418 720 739 1032
Size: 418 302 19 293
Fragments arranged by size:
418 302 293 19
BanII G_rGCy'C
Cuts at: 0 372 448 1032
Size: 372 76 584
Fragments arranged by size:
584 372 76
BfrBI ATG'CAT
Cuts at: 0 3 525 1032
Size: 3 522 507
Fragments arranged by size:
522 507 3
BglI GCCn_nnn'nGGC
Cuts at: 0 922 1032
Size: 922 110
BlpI GC'TnA_GC
Cuts at: 0 199 1032
Size: 199 833
Bme1580I G_kGCm'C
Cuts at: 0 57 448 563 725 742 1022 1032
Size: 57 391 115 162 17 280 10
Fragments arranged by size:
391 280 162 115 57 17 10
BmgBI CAC'GTC
Cuts at: 0 171 1032
Size: 171 861
BmrI ACTGGGnnnn_n'
Cuts at: 0 854 1032
Size: 854 178
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 472 860 1032
Size: 160 312 388 172
Fragments arranged by size:
388 312 172 160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 658 1032
Size: 658 374
BsaI GGTCTCn'nnnn_
Cuts at: 0 136 1032
Size: 136 896
BsaBI GATnn'nnATC
Cuts at: 0 22 1032
Size: 22 1010
BsaHI Gr'CG_yC
Cuts at: 0 80 757 1032
Size: 80 677 275
Fragments arranged by size:
677 275 80
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 571 601 877 907 1032
Size: 571 30 276 30 125
Fragments arranged by size:
571 276 125 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 807 810 1032
Size: 807 3 222
Fragments arranged by size:
807 222 3
BseYI C'CCAG_C
Cuts at: 0 504 562 790 829 834 1032
Size: 504 58 228 39 5 198
Fragments arranged by size:
504 228 198 58 39 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 1032
Size: 193 839
BsiEI CG_ry'CG
Cuts at: 0 193 1032
Size: 193 839
BsmI GAATG_Cn'
Cuts at: 0 105 1032
Size: 105 927
Bsp1286I G_dGCh'C
Cuts at: 0 57 372 448 563 725 742 1022 1032
Size: 57 315 76 115 162 17 280 10
Fragments arranged by size:
315 280 162 115 76 57 17 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 130 165 1032
Size: 130 35 867
Fragments arranged by size:
867 130 35
BstAPI GCAn_nnn'nTGC
Cuts at: 0 122 1032
Size: 122 910
BstXI CCAn_nnnn'nTGG
Cuts at: 0 93 1032
Size: 93 939
BtgI C'CryG_G
Cuts at: 0 339 716 849 867 1032
Size: 339 377 133 18 165
Fragments arranged by size:
377 339 165 133 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 513 615 1032
Size: 513 102 417
Fragments arranged by size:
513 417 102
DraI TTT'AAA
Cuts at: 0 261 1032
Size: 261 771
DrdI GACnn_nn'nnGTC
Cuts at: 0 175 1032
Size: 175 857
EaeI y'GGCC_r
Cuts at: 0 190 1032
Size: 190 842
EagI C'GGCC_G
Cuts at: 0 190 1032
Size: 190 842
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 669 1032
Size: 669 363
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 472 625 860 1032
Size: 160 312 153 235 172
Fragments arranged by size:
312 235 172 160 153
EcoNI CCTnn'n_nnAGG
Cuts at: 0 310 1032
Size: 310 722
EcoO109I rG'GnC_Cy
Cuts at: 0 315 444 445 1032
Size: 315 129 1 587
Fragments arranged by size:
587 315 129 1
EcoRI G'AATT_C
Cuts at: 0 406 1032
Size: 406 626
HaeII r_GCGC'y
Cuts at: 0 1013 1032
Size: 1013 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 754 786 1032
Size: 754 32 246
Fragments arranged by size:
754 246 32
HincII GTy'rAC
Cuts at: 0 1005 1032
Size: 1005 27
MslI CAynn'nnrTG
Cuts at: 0 91 622 1032
Size: 91 531 410
Fragments arranged by size:
531 410 91
MspA1I CmG'CkG
Cuts at: 0 195 341 752 934 1032
Size: 195 146 411 182 98
Fragments arranged by size:
411 195 182 146 98
NcoI C'CATG_G
Cuts at: 0 849 867 1032
Size: 849 18 165
Fragments arranged by size:
849 165 18
NsiI A_TGCA'T
Cuts at: 0 5 527 1032
Size: 5 522 505
Fragments arranged by size:
522 505 5
PasI CC'CwG_GG
Cuts at: 0 250 1032
Size: 250 782
PflMI CCAn_nnn'nTGG
Cuts at: 0 850 1032
Size: 850 182
PpuMI rG'GwC_Cy
Cuts at: 0 315 1032
Size: 315 717
PshAI GACnn'nnGTC
Cuts at: 0 313 1032
Size: 313 719
PspOMI G'GGCC_C
Cuts at: 0 444 1032
Size: 444 588
PstI C_TGCA'G
Cuts at: 0 965 1032
Size: 965 67
PvuII CAG'CTG
Cuts at: 0 934 1032
Size: 934 98
SacII CC_GC'GG
Cuts at: 0 342 1032
Size: 342 690
SfcI C'TryA_G
Cuts at: 0 961 1032
Size: 961 71
SmlI C'TyrA_G
Cuts at: 0 673 1032
Size: 673 359
StyI C'CwwG_G
Cuts at: 0 849 867 1032
Size: 849 18 165
Fragments arranged by size:
849 165 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 898 1032
Size: 898 134
TatI w'GTAC_w
Cuts at: 0 569 1032
Size: 569 463
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 877 909 1032
Size: 877 32 123
Fragments arranged by size:
877 123 32
Tth111I GACn'n_nGTC
Cuts at: 0 232 1032
Size: 232 800
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 90 1032
Size: 90 942
Enzymes that do cut:
AccI AcuI AleI ApaI ApoI BanI BanII BfrBI
BglI BlpI Bme1580I BmgBI BmrI BpmI BpuEI BsaI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsmI
Bsp1286I BspMI BstAPI BstXI BtgI BtgZI DraI DrdI
EaeI EagI EciI Eco57MI EcoNI EcoO109I EcoRI HaeII
Hin4I HincII MslI MspA1I NcoI NsiI PasI PflMI
PpuMI PshAI PspOMI PstI PvuII SacII SfcI SmlI
StyI TaqII TatI TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI
BciVI BclI BglII BmtI BplI Bpu10I BsaAI BsaWI
BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I
BtsI ClaI CspCI DraIII EarI EcoICRI EcoRV FalI
FseI FspI FspAI HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NspI PacI PciI PfoI PmeI PmlI
PpiI PsiI PspXI PsrI PvuI RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI XbaI XhoI XmaI XmnI ZraI