Restriction map for
pFN21AB8357
##################################
# NO CUT ENZYMES ( 144 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BanI BbeI BbrPI BbsI BciVI
BclI BfaI BfrI BglI BglII
BlnI BpuAI BsaAI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CfoI ClaI CpoI
DraI DraIII EagI EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HgaI HhaI HinP1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I MaeI MfeI MluI MroI
MseI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccI 52 GTCTAC GT^[AC][GT]AC
AccII 68 CGCG CG^CG
AcsI 400 GAATTT [GA]^AATT[CT]
AhdI 181 GACAGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 400 GAATTT [GA]^AATT[CT]
AspEI 181 GACAGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 524 CCCGAG C^[CT]CG[GA]G
BalI 685 TGGCCA TGG^CCA
BamHI 631 GGATCC G^GATCC
BanII 196 GAGCTC G[GA]GC[CT]^C
BbvCI 135 GCTGAGG CCTCAGC(-5/-2)
BcgI 263 GCAGGTACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 213 CCCGG CC^[GC]GG
BlpI 552 GCTGAGC GC^T[ACGT]AGC
BpmI 175 CTGGAG CTGGAG(16/14)
Bpu1102I 552 GCTGAGC GC^T[ACGT]AGC
BsaBI 276 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 4 GAGGAG GAGGAG(10/8)
BsmAI 722 GAGAC GTCTC(1/5)
BsoBI 524 CCCGAG C^[CT]CG[GA]G
BspMI 263 GCAGGT ACCTGC(4/8)
BsrBI 9 GAGCGG CCGCTC(-3/-3)
BsrDI 362 CATTGC GCAATG(2/0)
BsrFI 131 GCCGGC [GA]^CCGG[CT]
BstF5I 60 CATCC GGATG(2/0)
BstUI 68 CGCG CG^CG
BstYI 631 GGATCC [GA]^GATC[CT]
CelII 552 GCTGAGC GC^T[ACGT]AGC
Cfr10I 131 GCCGGC [GA]^CCGG[CT]
DrdI 43 GACGTCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 181 GACAGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 568 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 429 CCAAGG C^C[AT][AT]GG
FokI 60 CATCC GGATG(9/13)
KspI 67 CCGCGG CCGC^GG
MaeIII 250 GTGAC ^GT[ACGT]AC
MamI 276 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 631 GGATCC [GA]^GATC[CT]
MluNI 685 TGGCCA TGG^CCA
MscI 685 TGGCCA TGG^CCA
MslI 234 CACAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 68 CGCG CG^CG
NaeI 131 GCCGGC GCC^GGC
NciI 213 CCCGG CC^[GC]GG
NgoMIV 131 GCCGGC G^CCGGC
SacI 196 GAGCTC GAGCT^C
SacII 67 CCGCGG CCGC^GG
StyI 429 CCAAGG C^C[AT][AT]GG
TaqI 284 TCGA T^CGA
Tsp45I 250 GTGAC ^GT[GC]AC
TthHB8I 284 TCGA T^CGA
XcmI 766 CCAGGCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 631 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AatII 43 GACGTC GACGT^C
186 GACGTC
AcyI 43 GACGTC G[GA]^CG[CT]C
186 GACGTC
BsaHI 43 GACGTC G[GA]^CG[CT]C
186 GACGTC
BsmFI 153 GGGAC GGGAC(10/14)
329 GGGAC
DraII 209 AGGCCCC [GA]G^G[ACGT]CC[CT]
432 AGGCCCT
DsaI 67 CCGCGG C^C[GA][CT]GG
81 CCGTGG
EaeI 129 TGGCCG [CT]^GGCC[GA]
685 TGGCCA
EcoO109I 209 AGGCCCC [GA]G^G[ACGT]CC[CT]
432 AGGCCCT
FauI 11 GCGGG CCCGC(4/6)
69 GCGGG
Hin1I 43 GACGTC G[GA]^CG[CT]C
186 GACGTC
HphI 337 GGTGA GGTGA(8/7)
384 GGTGA
NlaIII 122 CATG CATG^
492 CATG
PleI 19 GAGTC GAGTC(4/5)
527 GAGTC
SfcI 87 CTACAG C^T[GA][CT]AG
737 CTACAG
TfiI 256 GATTC G^A[AT]TC
519 GAATC
Tsp509I 318 AATT ^AATT
401 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BbvI BmyI BsaJI BsiHKAI
BsiYI BslI Bsp1286I BsrI BssKI
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII Fnu4HI HaeIII HapII HinfI
HpaII ItaI MaeII MboI MboII
MnlI MspA1I MspI MvaI MwoI
NdeII NlaIV PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI TseI TspRI