(Circular) (Six-base) MAPSORT of: pFN21AB8357.seq Check: 9939 from: 1 to: 5494 pFN21AB8357 5494 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2052 2195 3304 278 Size: 53 83 186 936 516 143 1109 2468 Fragments arranged by size: 2468 1109 936 516 186 143 83 53 AccI GT'mk_AC Cuts at: 1937 2058 2837 1937 Size: 121 779 4594 Fragments arranged by size: 4594 779 121 Acc65I G'GTAC_C Cuts at: 2815 2815 Size: 5494 AclI AA'CG_TT Cuts at: 1563 3621 3994 1563 Size: 2058 373 3063 Fragments arranged by size: 3063 2058 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3557 4941 5209 1632 Size: 1925 1384 268 1917 Fragments arranged by size: 1925 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5297 AflIII A'CryG_T Cuts at: 1203 4393 5370 1203 Size: 3190 977 1327 Fragments arranged by size: 3190 1327 977 AgeI A'CCGG_T Cuts at: 4325 4325 Size: 5494 AhdI GACnn_n'nnGTC Cuts at: 2191 4223 2191 Size: 2032 3462 AleI CACnn'nnGTG Cuts at: 1166 2243 1166 Size: 1077 4417 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5494 AlwNI CAG_nnn'CTG Cuts at: 3237 4809 3237 Size: 1572 3922 ApaLI G'TGCA_C Cuts at: 3551 4707 3551 Size: 1156 4338 ApoI r'AATT_y Cuts at: 1493 2405 2803 3029 3181 5290 1493 Size: 912 398 226 152 2109 1697 Fragments arranged by size: 2109 1697 912 398 226 152 AseI AT'TA_AT Cuts at: 160 1032 4048 160 Size: 872 3016 1606 Fragments arranged by size: 3016 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5494 AvaI C'yCGr_G Cuts at: 1870 1943 2529 2819 1870 Size: 73 586 290 4545 Fragments arranged by size: 4545 586 290 73 BamHI G'GATC_C Cuts at: 2636 2824 3191 2636 Size: 188 367 4939 Fragments arranged by size: 4939 367 188 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2815 4270 618 Size: 325 217 627 1028 1455 1842 Fragments arranged by size: 1842 1455 1028 627 325 217 BanII G_rGCy'C Cuts at: 1391 2205 2813 1391 Size: 814 608 4072 Fragments arranged by size: 4072 814 608 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4523 BbvCI CC'TCA_GC Cuts at: 2141 2141 Size: 5494 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2257 2291 3685 3719 1903 Size: 34 320 34 1394 34 3678 Fragments arranged by size: 3678 1394 320 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3386 4596 3386 Size: 1210 4284 BclI T'GATC_A Cuts at: 1548 1548 Size: 5494 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4105 136 Size: 107 122 71 631 605 2433 1525 Fragments arranged by size: 2433 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5489 5489 Size: 5494 BlpI GC'TnA_GC Cuts at: 2558 2911 2558 Size: 353 5141 Bme1580I G_kGCm'C Cuts at: 1790 3555 4711 1790 Size: 1765 1156 2573 Fragments arranged by size: 2573 1765 1156 BmgBI CAC'GTC Cuts at: 1318 2056 1318 Size: 738 4756 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2658 3225 4183 Size: 416 53 83 399 24 1241 567 958 Cuts at: 4183 442 Size: 1753 Fragments arranged by size: 1753 1241 958 567 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5494 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2201 4154 1239 Size: 962 1953 2579 Fragments arranged by size: 2579 1953 962 Bpu10I CC'TnA_GC Cuts at: 2141 2211 2141 Size: 70 5424 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3619 4484 4782 3619 Size: 865 298 4331 Fragments arranged by size: 4331 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4157 882 Size: 617 2658 2219 Fragments arranged by size: 2658 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3285 493 Size: 711 2081 2702 Fragments arranged by size: 2702 2081 711 BsaBI GATnn'nnATC Cuts at: 2285 5488 2285 Size: 3203 2291 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2049 2192 3301 3683 Size: 53 83 186 936 516 143 1109 382 Cuts at: 3683 4318 275 Size: 635 1451 Fragments arranged by size: 1451 1109 936 635 516 382 186 143 83 53 BsaWI w'CCGG_w Cuts at: 3927 4325 4599 4746 5157 3927 Size: 398 274 147 411 4264 Fragments arranged by size: 4264 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2003 2033 1178 Size: 30 165 30 600 30 4639 Fragments arranged by size: 4639 600 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 1186 Size: 838 4656 BseYI C'CCAG_C Cuts at: 2519 2766 2781 4697 5193 2519 Size: 247 15 1916 496 2820 Fragments arranged by size: 2820 1916 496 247 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4921 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2944 3705 4733 1540 Size: 275 186 943 761 1028 2301 Fragments arranged by size: 2301 1028 943 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2034 2205 2680 3555 3640 4711 2034 Size: 171 475 875 85 1071 2817 Fragments arranged by size: 2817 1071 875 475 171 85 BsmI GAATG_Cn' Cuts at: 3005 3098 3005 Size: 93 5401 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2205 2680 2813 3555 3640 4711 Size: 399 244 171 475 133 742 85 1071 Cuts at: 4711 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 475 399 244 171 133 85 BspEI T'CCGG_A Cuts at: 5157 5157 Size: 5494 BspHI T'CATG_A Cuts at: 3383 5113 3383 Size: 1730 3764 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2259 2849 844 Size: 1415 590 3489 Fragments arranged by size: 3489 1415 590 BsrBI CCG'CTC Cuts at: 1394 1823 2016 3381 1394 Size: 429 193 1365 3507 Fragments arranged by size: 3507 1365 429 193 BsrDI GCAATG_nn' Cuts at: 65 1248 2366 3989 4163 65 Size: 1183 1118 1623 174 1396 Fragments arranged by size: 1623 1396 1183 1118 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2136 4138 4325 1700 Size: 110 326 2002 187 2869 Fragments arranged by size: 2869 2002 326 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5494 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5494 BssSI C'ACGA_G Cuts at: 3554 4566 3554 Size: 1012 4482 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5494 BstBI TT'CG_AA Cuts at: 4298 4298 Size: 5494 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5494 BstYI r'GATC_y Cuts at: 1972 2636 2824 3191 3576 3593 5045 5131 5143 Size: 664 188 367 385 17 1452 86 12 Cuts at: 5143 5489 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 664 385 367 346 188 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2072 2086 513 Size: 552 939 68 14 3921 Fragments arranged by size: 3921 939 552 68 14 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2473 487 Size: 683 56 1247 3508 Fragments arranged by size: 3508 1247 683 56 BtsI GCAGTG_nn' Cuts at: 2148 2405 2709 3010 3804 3824 2148 Size: 257 304 301 794 20 3818 Fragments arranged by size: 3818 794 304 301 257 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5494 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5459 DraI TTT'AAA Cuts at: 2798 2798 Size: 5494 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2054 4501 809 Size: 507 738 2447 1802 Fragments arranged by size: 2447 1802 738 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2134 2690 2941 Size: 54 1425 50 275 18 304 556 251 Cuts at: 2941 3830 5459 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 556 304 275 251 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2941 1537 Size: 275 1129 4090 Fragments arranged by size: 4090 1129 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3424 1353 Size: 2071 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4081 4455 4601 387 Size: 1064 138 2492 374 146 1280 Fragments arranged by size: 2492 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2203 2203 Size: 5494 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2201 3557 4154 4941 5209 1239 Size: 393 569 1356 597 787 268 1524 Fragments arranged by size: 1524 1356 787 597 569 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2577 2577 Size: 5494 EcoO109I rG'GnC_Cy Cuts at: 2215 2438 2215 Size: 223 5271 EcoRI G'AATT_C Cuts at: 2803 3181 2803 Size: 378 5116 FspI TGC'GCA Cuts at: 4000 4000 Size: 5494 HaeII r_GCGC'y Cuts at: 1678 4641 1678 Size: 2963 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2108 2140 2177 2209 2628 Size: 32 135 32 536 32 37 32 419 Cuts at: 2628 2660 4135 4167 4209 4241 1373 Size: 32 1475 32 42 32 2626 Fragments arranged by size: 2626 1475 536 419 135 42 37 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2838 3084 669 Size: 988 281 900 246 3079 Fragments arranged by size: 3079 988 900 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5494 HpaI GTT'AAC Cuts at: 3084 3084 Size: 5494 KpnI G_GTAC'C Cuts at: 2819 2819 Size: 5494 MfeI C'AATT_G Cuts at: 3093 3093 Size: 5494 MluI A'CGCG_T Cuts at: 5370 5370 Size: 5494 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4608 4792 1305 Size: 3303 184 2007 Fragments arranged by size: 3303 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2692 10 Size: 54 1425 343 860 2812 Fragments arranged by size: 2812 1425 860 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2243 3452 3811 3970 518 Size: 648 1077 1209 359 159 2042 Fragments arranged by size: 2042 1209 1077 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2074 2113 2243 2519 2910 3587 4735 4980 Size: 468 39 130 276 391 677 1148 245 Cuts at: 4980 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 468 391 276 245 130 39 NaeI GCC'GGC Cuts at: 2138 2138 Size: 5494 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4003 Fragments arranged by size: 4003 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5494 NgoMIV G'CCGG_C Cuts at: 2136 2136 Size: 5494 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5494 NotI GC'GGCC_GC Cuts at: 2941 2941 Size: 5494 NspI r_CATG'y Cuts at: 1132 1229 2852 4397 1132 Size: 97 1623 1545 2229 Fragments arranged by size: 2229 1623 1545 97 PasI CC'CwG_GG Cuts at: 1348 2579 2612 1348 Size: 1231 33 4230 Fragments arranged by size: 4230 1231 33 PciI A'CATG_T Cuts at: 4393 4393 Size: 5494 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5494 PmeI GTTT'AAAC Cuts at: 2798 2798 Size: 5494 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3563 3595 5076 5108 3563 Size: 32 1481 32 3949 Fragments arranged by size: 3949 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5494 PsiI TTA'TAA Cuts at: 3064 3064 Size: 5494 PstI C_TGCA'G Cuts at: 830 2846 830 Size: 2016 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5494 PvuII CAG'CTG Cuts at: 2113 2243 2519 2113 Size: 130 276 5088 Fragments arranged by size: 5088 276 130 SacI G_AGCT'C Cuts at: 2205 2205 Size: 5494 SacII CC_GC'GG Cuts at: 2075 2075 Size: 5494 SalI G'TCGA_C Cuts at: 1936 2836 1936 Size: 900 4594 SbfI CC_TGCA'GG Cuts at: 2846 2846 Size: 5494 ScaI AGT'ACT Cuts at: 3742 5454 3742 Size: 1712 3782 SfcI C'TryA_G Cuts at: 826 1045 2092 2742 2842 3977 4658 4849 826 Size: 219 1047 650 100 1135 681 191 1471 Fragments arranged by size: 1471 1135 1047 681 650 219 191 100 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5494 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5494 SmaI CCC'GGG Cuts at: 1872 2821 1872 Size: 949 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3598 4499 4761 820 Size: 197 926 1655 901 262 1553 Fragments arranged by size: 1655 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3285 493 Size: 2792 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5494 SphI G_CATG'C Cuts at: 1132 2852 1132 Size: 1720 3774 SspI AAT'ATT Cuts at: 5 52 3418 5 Size: 47 3366 2081 Fragments arranged by size: 3366 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5494 StyI C'CwwG_G Cuts at: 513 1065 2004 2434 2933 513 Size: 552 939 430 499 3074 Fragments arranged by size: 3074 939 552 499 430 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2352 2377 3521 3538 3691 3876 4321 Size: 1255 772 25 1144 17 153 185 445 Cuts at: 4321 325 Size: 1498 Fragments arranged by size: 1498 1255 1144 772 445 185 153 25 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3740 5452 96 Size: 274 80 33 51 1443 1763 1712 138 Fragments arranged by size: 1763 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5454 5486 5454 Size: 32 5462 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5494 XbaI T'CTAG_A Cuts at: 2830 2830 Size: 5494 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2778 2778 Size: 5494 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5494 XmaI C'CCGG_G Cuts at: 1870 2819 1870 Size: 949 4545 XmnI GAAnn'nnTTC Cuts at: 3623 3623 Size: 5494 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2050 2193 3302 276 Size: 53 83 186 936 516 143 1109 2468 Fragments arranged by size: 2468 1109 936 516 186 143 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI