(Circular) (Six-base) MAPSORT of: pFN21AB8356.seq Check: 6025 from: 1 to: 5848 pFN21AB8356 5848 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2623 2623 Size: 5848 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3658 278 Size: 53 83 186 936 2122 2468 Fragments arranged by size: 2468 2122 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2571 3084 3191 1937 Size: 634 513 107 4594 Fragments arranged by size: 4594 634 513 107 Acc65I G'GTAC_C Cuts at: 3169 3169 Size: 5848 AclI AA'CG_TT Cuts at: 1563 3975 4348 1563 Size: 2412 373 3063 Fragments arranged by size: 3063 2412 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3911 5295 5563 1632 Size: 2279 1384 268 1917 Fragments arranged by size: 2279 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5651 AflIII A'CryG_T Cuts at: 1203 2940 4747 5724 1203 Size: 1737 1807 977 1327 Fragments arranged by size: 1807 1737 1327 977 AgeI A'CCGG_T Cuts at: 4679 4679 Size: 5848 AhdI GACnn_n'nnGTC Cuts at: 4577 4577 Size: 5848 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5848 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3074 1184 Size: 1890 3958 AlwNI CAG_nnn'CTG Cuts at: 3591 5163 3591 Size: 1572 4276 ApaI G_GGCC'C Cuts at: 2039 2039 Size: 5848 ApaLI G'TGCA_C Cuts at: 3905 5061 3905 Size: 1156 4692 ApoI r'AATT_y Cuts at: 1493 3157 3383 3535 5644 1493 Size: 1664 226 152 2109 1697 Fragments arranged by size: 2109 1697 1664 226 152 AseI AT'TA_AT Cuts at: 160 1032 4402 160 Size: 872 3370 1606 Fragments arranged by size: 3370 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5848 AvaI C'yCGr_G Cuts at: 1870 1943 2094 2615 3173 1870 Size: 73 151 521 558 4545 Fragments arranged by size: 4545 558 521 151 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3013 3046 3013 Size: 33 5815 BamHI G'GATC_C Cuts at: 3178 3545 3178 Size: 367 5481 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3169 4624 618 Size: 325 217 627 1382 1455 1842 Fragments arranged by size: 1842 1455 1382 627 325 217 BanII G_rGCy'C Cuts at: 1391 2039 2550 3167 1391 Size: 648 511 617 4072 Fragments arranged by size: 4072 648 617 511 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4877 BbvCI CC'TCA_GC Cuts at: 2384 2604 2910 2384 Size: 220 306 5322 Fragments arranged by size: 5322 306 220 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2387 2421 4039 4073 1903 Size: 34 450 34 1618 34 3678 Fragments arranged by size: 3678 1618 450 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3740 4950 3740 Size: 1210 4638 BclI T'GATC_A Cuts at: 1548 1548 Size: 5848 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2730 4459 136 Size: 107 122 71 631 605 1058 1729 1525 Fragments arranged by size: 1729 1525 1058 631 605 122 107 71 BglII A'GATC_T Cuts at: 3007 5843 3007 Size: 2836 3012 BlpI GC'TnA_GC Cuts at: 3265 3265 Size: 5848 Bme1580I G_kGCm'C Cuts at: 1790 2039 2239 3909 5065 1790 Size: 249 200 1670 1156 2573 Fragments arranged by size: 2573 1670 1156 249 200 BmgBI CAC'GTC Cuts at: 1318 2374 1318 Size: 1056 4792 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2720 3579 4537 Size: 416 53 83 399 24 1303 859 958 Cuts at: 4537 442 Size: 1753 Fragments arranged by size: 1753 1303 958 859 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5848 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2545 4508 1239 Size: 1306 1963 2579 Fragments arranged by size: 2579 1963 1306 Bpu10I CC'TnA_GC Cuts at: 2384 2604 2910 2384 Size: 220 306 5322 Fragments arranged by size: 5322 306 220 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2169 3128 3973 4838 5136 2169 Size: 959 845 865 298 2881 Fragments arranged by size: 2881 959 865 845 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4511 882 Size: 617 3012 2219 Fragments arranged by size: 3012 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3639 493 Size: 711 2435 2702 Fragments arranged by size: 2702 2435 711 BsaBI GATnn'nnATC Cuts at: 5842 5842 Size: 5848 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3655 4037 4672 275 Size: 53 83 186 936 2122 382 635 1451 Fragments arranged by size: 2122 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2055 4281 4679 4953 5100 5511 2055 Size: 2226 398 274 147 411 2392 Fragments arranged by size: 2392 2226 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2358 2388 2476 2506 2619 Size: 30 165 30 955 30 88 30 113 Cuts at: 2619 2649 1178 Size: 30 4377 Fragments arranged by size: 4377 955 165 113 88 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2379 2497 2558 2572 2610 2621 2927 1186 Size: 1193 118 61 14 38 11 306 4107 Fragments arranged by size: 4107 1193 306 118 61 38 14 11 BseYI C'CCAG_C Cuts at: 2038 5051 5547 2038 Size: 3013 496 2339 Fragments arranged by size: 3013 2339 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2651 2941 1163 Size: 573 915 290 4070 Fragments arranged by size: 4070 915 573 290 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3298 4059 5087 1540 Size: 275 186 1297 761 1028 2301 Fragments arranged by size: 2301 1297 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2919 3909 3994 5065 2919 Size: 990 85 1071 3702 Fragments arranged by size: 3702 1071 990 85 BsmI GAATG_Cn' Cuts at: 3051 3359 3452 3051 Size: 308 93 5447 Fragments arranged by size: 5447 308 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2039 2239 2550 2919 3167 3909 3994 Size: 399 249 200 311 369 248 742 85 Cuts at: 3994 5065 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 369 311 249 248 200 85 BspEI T'CCGG_A Cuts at: 2055 5511 2055 Size: 3456 2392 BspHI T'CATG_A Cuts at: 3737 5467 3737 Size: 1730 4118 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2623 3203 844 Size: 1779 580 3489 Fragments arranged by size: 3489 1779 580 BsrBI CCG'CTC Cuts at: 1394 1823 3735 1394 Size: 429 1912 3507 Fragments arranged by size: 3507 1912 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4343 4517 65 Size: 1183 3095 174 1396 Fragments arranged by size: 3095 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4492 4679 1700 Size: 110 2682 187 2869 Fragments arranged by size: 2869 2682 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5848 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5848 BssSI C'ACGA_G Cuts at: 3908 4920 3908 Size: 1012 4836 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5848 BstBI TT'CG_AA Cuts at: 4652 4652 Size: 5848 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5848 BstXI CCAn_nnnn'nTGG Cuts at: 2341 2341 Size: 5848 BstYI r'GATC_y Cuts at: 1972 2988 3007 3178 3545 3930 3947 5399 5485 Size: 1016 19 171 367 385 17 1452 86 Cuts at: 5485 5497 5843 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1016 385 367 346 171 86 19 17 12 Bsu36I CC'TnA_GG Cuts at: 2301 2301 Size: 5848 BtgI C'CryG_G Cuts at: 513 1065 2004 2464 513 Size: 552 939 460 3897 Fragments arranged by size: 3897 939 552 460 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5109 Fragments arranged by size: 5109 683 56 BtsI GCAGTG_nn' Cuts at: 2114 2762 3364 4158 4178 2114 Size: 648 602 794 20 3784 Fragments arranged by size: 3784 794 648 602 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5848 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5813 DraI TTT'AAA Cuts at: 3152 3152 Size: 5848 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4855 809 Size: 507 3539 1802 Fragments arranged by size: 3539 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2823 2863 3295 Size: 54 1425 50 275 18 993 40 432 Cuts at: 3295 4184 5813 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 993 889 432 275 54 50 43 40 18 EagI C'GGCC_G Cuts at: 1537 1812 3295 1537 Size: 275 1483 4090 Fragments arranged by size: 4090 1483 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2799 3778 1353 Size: 1446 979 3423 Fragments arranged by size: 3423 1446 979 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4435 4809 4955 387 Size: 1064 138 2846 374 146 1280 Fragments arranged by size: 2846 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2545 3911 4508 5295 5563 1239 Size: 393 913 1366 597 787 268 1524 Fragments arranged by size: 1524 1366 913 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2035 2169 2035 Size: 134 5714 EcoRI G'AATT_C Cuts at: 3157 3535 3157 Size: 378 5470 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2677 2677 Size: 5848 FspI TGC'GCA Cuts at: 4354 4354 Size: 5848 HaeII r_GCGC'y Cuts at: 1678 2052 2659 4995 1678 Size: 374 607 2336 2531 Fragments arranged by size: 2531 2336 607 374 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4489 4521 4563 4595 1373 Size: 32 135 32 2917 32 42 32 2626 Fragments arranged by size: 2917 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3192 3438 669 Size: 988 281 1254 246 3079 Fragments arranged by size: 3079 1254 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5848 HpaI GTT'AAC Cuts at: 3438 3438 Size: 5848 KpnI G_GTAC'C Cuts at: 3173 3173 Size: 5848 MfeI C'AATT_G Cuts at: 3447 3447 Size: 5848 MluI A'CGCG_T Cuts at: 5724 5724 Size: 5848 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4962 5146 1305 Size: 3657 184 2007 Fragments arranged by size: 3657 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2825 10 Size: 54 1425 343 993 3033 Fragments arranged by size: 3033 1425 993 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2274 3056 3806 4165 4324 518 Size: 648 1108 782 750 359 159 2042 Fragments arranged by size: 2042 1108 782 750 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2586 3264 3941 5089 5334 1606 Size: 980 678 677 1148 245 2120 Fragments arranged by size: 2120 1148 980 678 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4357 Fragments arranged by size: 4357 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5848 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5848 NotI GC'GGCC_GC Cuts at: 3295 3295 Size: 5848 NspI r_CATG'y Cuts at: 1132 1229 2944 3206 4751 1132 Size: 97 1715 262 1545 2229 Fragments arranged by size: 2229 1715 1545 262 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5848 PciI A'CATG_T Cuts at: 2940 4747 2940 Size: 1807 4041 PflMI CCAn_nnn'nTGG Cuts at: 1108 2523 1108 Size: 1415 4433 PmeI GTTT'AAAC Cuts at: 3152 3152 Size: 5848 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3917 3949 5430 5462 3917 Size: 32 1481 32 4303 Fragments arranged by size: 4303 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2169 2169 Size: 5848 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5848 PsiI TTA'TAA Cuts at: 3418 3418 Size: 5848 PspOMI G'GGCC_C Cuts at: 2035 2035 Size: 5848 PstI C_TGCA'G Cuts at: 830 3200 830 Size: 2370 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5848 SalI G'TCGA_C Cuts at: 1936 3190 1936 Size: 1254 4594 SanDI GG'GwC_CC Cuts at: 2169 2169 Size: 5848 SbfI CC_TGCA'GG Cuts at: 3200 3200 Size: 5848 ScaI AGT'ACT Cuts at: 4096 5808 4096 Size: 1712 4136 SexAI A'CCwGG_T Cuts at: 2499 2499 Size: 5848 SfcI C'TryA_G Cuts at: 826 1045 2148 2923 3196 4331 5012 5203 826 Size: 219 1103 775 273 1135 681 191 1471 Fragments arranged by size: 1471 1135 1103 775 681 273 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5848 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5848 SmaI CCC'GGG Cuts at: 1872 3175 1872 Size: 1303 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2184 3143 3952 4853 5115 820 Size: 197 926 241 959 809 901 262 1553 Fragments arranged by size: 1553 959 926 901 809 262 241 197 SnaBI TAC'GTA Cuts at: 493 3639 493 Size: 3146 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5848 SphI G_CATG'C Cuts at: 1132 3206 1132 Size: 2074 3774 SspI AAT'ATT Cuts at: 5 52 3772 5 Size: 47 3720 2081 Fragments arranged by size: 3720 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5848 StyI C'CwwG_G Cuts at: 513 1065 2004 2517 3287 513 Size: 552 939 513 770 3074 Fragments arranged by size: 3074 939 770 552 513 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3875 3892 4045 4230 4675 325 Size: 1255 2295 17 153 185 445 1498 Fragments arranged by size: 2295 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4094 5806 96 Size: 274 80 33 51 1443 2117 1712 138 Fragments arranged by size: 2117 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2358 2390 2476 2508 2619 2651 2762 2794 5808 Size: 32 86 32 111 32 111 32 3014 Cuts at: 5808 5840 2358 Size: 32 2366 Fragments arranged by size: 3014 2366 111 111 86 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5848 XbaI T'CTAG_A Cuts at: 3184 3184 Size: 5848 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2978 2999 2978 Size: 21 5827 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5848 XmaI C'CCGG_G Cuts at: 1870 3173 1870 Size: 1303 4545 XmnI GAAnn'nnTTC Cuts at: 3977 3977 Size: 5848 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3656 276 Size: 53 83 186 936 2122 2468 Fragments arranged by size: 2468 2122 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SapI SfiI SfoI SrfI SwaI