Restriction map for
pFN21AB8355
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BciVI
BclI BfrI BglII BlnI BsiEI
BsiWI BsmBI BsmI BspDI BspHI
BspLU11I BsrDI BssHII Bst1107I BstBI
BstYI BstZ17I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FbaI FspI HpaI KpnI KspI
MfeI MflI MluI MunI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI SspI
SwaI TfiI Tsp509I Tth111I XbaI
XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 340 GTCGAC GT^[AC][GT]AC
AccIII 1554 TCCGGA T^CCGGA
BalI 35 TGGCCA TGG^CCA
BbrPI 307 CACGTG CAC^GTG
BbvCI 1694 CCTCAGC CCTCAGC(-5/-2)
BcgI 1578 CGAAGGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1156 CTAG C^TAG
BglI 1183 GCCCCTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1051 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1051 GCTGAGC GC^T[ACGT]AGC
BsaBI 1887 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1554 TCCGGA T^CCGGA
BsgI 439 CTGCAC GTGCAG(16/14)
Bsp1407I 98 TGTACA T^GTACA
BspEI 1554 TCCGGA T^CCGGA
BspMI 1739 ACCTGC ACCTGC(4/8)
BsrFI 1198 GCCGGC [GA]^CCGG[CT]
BsrGI 98 TGTACA T^GTACA
BssSI 79 CTCGTG CACGAG(-5/-1)
BstAPI 441 GCACCGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1051 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1198 GCCGGC [GA]^CCGG[CT]
EaeI 35 TGGCCA [CT]^GGCC[GA]
FseI 1197 GGCCGGCC GGCCGG^CC
HincII 340 GTCGAC GT[CT]^[GA]AC
HindII 340 GTCGAC GT[CT]^[GA]AC
HindIII 130 AAGCTT A^AGCTT
MaeI 1156 CTAG C^TAG
MamI 1887 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 35 TGGCCA TGG^CCA
MroI 1554 TCCGGA T^CCGGA
MscI 35 TGGCCA TGG^CCA
MseI 530 TTAA T^TAA
MslI 784 CATCGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1198 GCCGGC GCC^GGC
NdeI 104 CATATG CA^TATG
NgoMIV 1198 GCCGGC G^CCGGC
NspI 1897 ACATGC [GA]CATG^[CT]
PflMI 13 CCATGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 307 CACGTG CAC^GTG
PmlI 307 CACGTG CAC^GTG
PpuMI 1774 GGGACCC [GA]G^G[AT]CC[CT]
SacI 1837 GAGCTC GAGCT^C
SalI 340 GTCGAC G^TCGAC
SbfI 86 CCTGCAGG CCTGCA^GG
SmaI 1238 CCCGGG CCC^GGG
Sse8387I 86 CCTGCAGG CCTGCA^GG
SspBI 98 TGTACA T^GTACA
Tru9I 530 TTAA T^TAA
Van91I 13 CCATGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1238 CCCGGG C^CCGGG
XmaI 1238 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AocI 1186 CCTCAGG CC^T[ACGT]AGG
1219 CCTCAGG
ApaI 1067 GGGCCC GGGCC^C
1487 GGGCCC
AvaI 1238 CCCGGG C^[CT]CG[GA]G
1503 CTCGGG
AvaII 1341 GGTCC G^G[AT]CC
1775 GGACC
BbeI 990 GGCGCC GGCGC^C
1230 GGCGCC
BbsI 370 GAAGAC GAAGAC(2/6)
498 GAAGAC
BpuAI 370 GAAGAC GAAGAC(2/6)
498 GAAGAC
BsaAI 244 TACGTG [CT]AC^GT[GA]
307 CACGTG
BsaWI 980 ACCGGA [AT]^CCGG[AT]
1554 TCCGGA
BsmFI 171 GGGAC GGGAC(10/14)
1774 GGGAC
BsoBI 1238 CCCGGG C^[CT]CG[GA]G
1503 CTCGGG
BstXI 302 CCAGCCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
359 CCAGCAGCGTGG
Bsu36I 1186 CCTCAGG CC^T[ACGT]AGG
1219 CCTCAGG
Eco81I 1186 CCTCAGG CC^T[ACGT]AGG
1219 CCTCAGG
EcoNI 1071 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1521 CCTGGCAGAGG
KasI 990 GGCGCC G^GCGCC
1230 GGCGCC
NarI 990 GGCGCC GG^CGCC
1230 GGCGCC
NcoI 13 CCATGG C^CATGG
1205 CCATGG
SapI 568 GAAGAGC GCTCTTC(1/4)
1834 GAAGAGC
SfoI 990 GGCGCC GGC^GCC
1230 GGCGCC
SmlI 127 CTCAAG C^T[CT][GA]AG
1702 CTTGAG
StuI 47 AGGCCT AGG^CCT
1190 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI BanI BanII BbvI BcnI
BmyI BpmI BsaHI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BsrBI BsrI BssKI BstEII
BstF5I BstNI BstPI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TseI Tsp45I TspRI TthHB8I