(Circular) (Six-base) MAPSORT of: pFN21AB8355.seq Check: 8874 from: 1 to: 6658 pFN21AB8355 6658 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3753 3753 Size: 6658 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4468 278 Size: 53 83 186 936 2932 2468 Fragments arranged by size: 2932 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2346 4001 1937 Size: 409 1655 4594 Fragments arranged by size: 4594 1655 409 Acc65I G'GTAC_C Cuts at: 3979 3979 Size: 6658 AclI AA'CG_TT Cuts at: 1563 4785 5158 1563 Size: 3222 373 3063 Fragments arranged by size: 3222 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3787 4721 6105 6373 1632 Size: 2155 934 1384 268 1917 Fragments arranged by size: 2155 1917 1384 934 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6461 AflIII A'CryG_T Cuts at: 1203 5557 6534 1203 Size: 4354 977 1327 Fragments arranged by size: 4354 1327 977 AgeI A'CCGG_T Cuts at: 5489 5489 Size: 6658 AhdI GACnn_n'nnGTC Cuts at: 5387 5387 Size: 6658 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6658 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2046 1184 Size: 862 5796 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3260 3292 3260 Size: 32 6626 AlwNI CAG_nnn'CTG Cuts at: 4401 5973 4401 Size: 1572 5086 ApaI G_GGCC'C Cuts at: 3076 3496 3076 Size: 420 6238 ApaLI G'TGCA_C Cuts at: 4715 5871 4715 Size: 1156 5502 ApoI r'AATT_y Cuts at: 1493 3967 4193 4345 6454 1493 Size: 2474 226 152 2109 1697 Fragments arranged by size: 2474 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5212 160 Size: 872 4180 1606 Fragments arranged by size: 4180 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6658 AvaI C'yCGr_G Cuts at: 1870 1943 3243 3508 3983 1870 Size: 73 1300 265 475 4545 Fragments arranged by size: 4545 1300 475 265 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3504 3537 3790 3823 3504 Size: 33 253 33 6339 Fragments arranged by size: 6339 253 33 33 BamHI G'GATC_C Cuts at: 3988 4355 3988 Size: 367 6291 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2390 2489 2995 3235 3535 Size: 325 217 627 603 99 506 240 300 Cuts at: 3535 3979 5434 618 Size: 444 1455 1842 Fragments arranged by size: 1842 1455 627 603 506 444 325 300 240 217 99 BanII G_rGCy'C Cuts at: 1391 2101 2484 3023 3076 3150 3222 3489 3496 Size: 710 383 539 53 74 72 267 7 Cuts at: 3496 3669 3741 3846 3977 1391 Size: 173 72 105 131 4072 Fragments arranged by size: 4072 710 539 383 267 173 131 105 74 72 72 53 7 BbeI G_GCGC'C Cuts at: 2999 3239 2999 Size: 240 6418 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2382 2510 928 Size: 971 483 128 5076 Fragments arranged by size: 5076 971 483 128 BbvCI CC'TCA_GC Cuts at: 3700 3700 Size: 6658 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3572 3606 4849 4883 1903 Size: 34 1635 34 1243 34 3678 Fragments arranged by size: 3678 1635 1243 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4550 5760 4550 Size: 1210 5448 BclI T'GATC_A Cuts at: 1548 1548 Size: 6658 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3194 5269 136 Size: 107 122 71 631 605 1522 2075 1525 Fragments arranged by size: 2075 1525 1522 631 605 122 107 71 BglII A'GATC_T Cuts at: 6653 6653 Size: 6658 BlpI GC'TnA_GC Cuts at: 3057 4075 3057 Size: 1018 5640 Bme1580I G_kGCm'C Cuts at: 1790 2492 3076 3496 3540 4719 5875 1790 Size: 702 584 420 44 1179 1156 2573 Fragments arranged by size: 2573 1179 1156 702 584 420 44 BmgBI CAC'GTC Cuts at: 1318 2350 1318 Size: 1032 5626 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2272 3914 4389 Size: 416 53 83 399 24 855 1642 475 Cuts at: 4389 5347 442 Size: 958 1753 Fragments arranged by size: 1753 1642 958 855 475 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6658 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2097 3106 2097 Size: 1009 5649 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2077 2416 3016 3461 3737 3870 5318 1239 Size: 838 339 600 445 276 133 1448 2579 Fragments arranged by size: 2579 1448 838 600 445 339 276 133 Bpu10I CC'TnA_GC Cuts at: 2319 2621 3621 3700 2319 Size: 302 1000 79 5277 Fragments arranged by size: 5277 1000 302 79 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2117 3728 4783 5648 5946 2117 Size: 1611 1055 865 298 2829 Fragments arranged by size: 2829 1611 1055 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2801 3131 3167 3755 5321 882 Size: 617 1302 330 36 588 1566 2219 Fragments arranged by size: 2219 1566 1302 617 588 330 36 BsaAI yAC'GTr Cuts at: 493 1204 2251 2314 4449 493 Size: 711 1047 63 2135 2702 Fragments arranged by size: 2702 2135 1047 711 63 BsaBI GATnn'nnATC Cuts at: 3896 6652 3896 Size: 2756 3902 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2996 3236 3336 4465 Size: 53 83 186 936 1463 240 100 1129 Cuts at: 4465 4847 5482 275 Size: 382 635 1451 Fragments arranged by size: 1463 1451 1129 936 635 382 240 186 100 83 53 BsaWI w'CCGG_w Cuts at: 2985 3559 5091 5489 5763 5910 6321 2985 Size: 574 1532 398 274 147 411 3322 Fragments arranged by size: 3322 1532 574 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3809 3839 1178 Size: 30 165 30 2406 30 3997 Fragments arranged by size: 3997 2406 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2603 2606 2681 2723 2813 2855 3082 3185 Size: 1417 3 75 42 90 42 227 103 Cuts at: 3185 3230 3725 3833 3836 3947 3950 1186 Size: 45 495 108 3 111 3 3894 Fragments arranged by size: 3894 1417 495 227 111 108 103 90 75 45 42 42 3 3 3 BseYI C'CCAG_C Cuts at: 2009 2862 3149 3906 5861 6357 2009 Size: 853 287 757 1955 496 2310 Fragments arranged by size: 2310 1955 853 757 496 287 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2429 1163 Size: 573 693 5392 Fragments arranged by size: 5392 693 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4108 4869 5897 1540 Size: 275 186 2107 761 1028 2301 Fragments arranged by size: 2301 2107 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2256 2715 3846 4719 4804 5875 2256 Size: 459 1131 873 85 1071 3039 Fragments arranged by size: 3039 1131 1071 873 459 85 BsmI GAATG_Cn' Cuts at: 4169 4262 4169 Size: 93 6565 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2101 2256 2484 2492 2715 3023 3076 Size: 399 311 155 228 8 223 308 53 Cuts at: 3076 3150 3222 3489 3496 3540 3669 3741 3846 Size: 74 72 267 7 44 129 72 105 Cuts at: 3846 3977 4719 4804 5875 1391 Size: 131 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 311 308 267 228 223 155 131 129 105 85 74 72 72 53 44 8 7 BspEI T'CCGG_A Cuts at: 3559 6321 3559 Size: 2762 3896 BspHI T'CATG_A Cuts at: 4547 6277 4547 Size: 1730 4928 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3753 4013 844 Size: 2909 260 3489 Fragments arranged by size: 3489 2909 260 BsrBI CCG'CTC Cuts at: 1394 1823 2596 2638 2653 2677 2692 3070 4545 Size: 429 773 42 15 24 15 378 1475 Cuts at: 4545 1394 Size: 3507 Fragments arranged by size: 3507 1475 773 429 378 42 24 15 15 BsrDI GCAATG_nn' Cuts at: 65 1248 5153 5327 65 Size: 1183 3905 174 1396 Fragments arranged by size: 3905 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3203 5302 5489 1700 Size: 110 1393 2099 187 2869 Fragments arranged by size: 2869 2099 1393 187 110 BsrGI T'GTAC_A Cuts at: 96 2103 96 Size: 2007 4651 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6658 BssSI C'ACGA_G Cuts at: 2084 4718 5730 2084 Size: 2634 1012 3012 Fragments arranged by size: 3012 2634 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2452 794 Size: 1658 5000 BstBI TT'CG_AA Cuts at: 5462 5462 Size: 6658 BstEII G'GTnAC_C Cuts at: 1184 2783 3085 3520 1184 Size: 1599 302 435 4322 Fragments arranged by size: 4322 1599 435 302 BstXI CCAn_nnnn'nTGG Cuts at: 2314 2371 2314 Size: 57 6601 BstYI r'GATC_y Cuts at: 1972 3988 4355 4740 4757 6209 6295 6307 6653 Size: 2016 367 385 17 1452 86 12 346 Cuts at: 6653 1972 Size: 1977 Fragments arranged by size: 2016 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3192 3225 3192 Size: 33 6625 BtgI C'CryG_G Cuts at: 513 1065 2004 2018 2484 3210 3315 3339 513 Size: 552 939 14 466 726 105 24 3832 Fragments arranged by size: 3832 939 726 552 466 105 24 14 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5919 Fragments arranged by size: 5919 683 56 BtsI GCAGTG_nn' Cuts at: 2221 3107 3378 3384 3566 4174 4968 4988 2221 Size: 886 271 6 182 608 794 20 3891 Fragments arranged by size: 3891 886 794 608 271 182 20 6 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6658 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6623 DraI TTT'AAA Cuts at: 3962 3962 Size: 6658 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5665 809 Size: 507 4349 1802 Fragments arranged by size: 4349 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 4105 4994 Size: 54 1425 50 275 18 210 2065 889 Cuts at: 4994 6623 8 Size: 1629 43 Fragments arranged by size: 2065 1629 1425 889 275 210 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4105 1537 Size: 275 2293 4090 Fragments arranged by size: 4090 2293 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2568 2739 2799 3834 3924 4588 1353 Size: 1215 171 60 1035 90 664 3423 Fragments arranged by size: 3423 1215 1035 664 171 90 60 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3849 5245 5619 5765 387 Size: 1064 138 2260 1396 374 146 1280 Fragments arranged by size: 2260 1396 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3844 3844 Size: 6658 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2077 2416 3016 3461 3737 3787 3870 Size: 393 445 339 600 445 276 50 83 Cuts at: 3870 4721 5318 6105 6373 1239 Size: 851 597 787 268 1524 Fragments arranged by size: 1524 851 787 600 597 445 445 393 339 276 268 83 50 EcoNI CCTnn'n_nnAGG Cuts at: 3080 3530 3080 Size: 450 6208 EcoO109I rG'GnC_Cy Cuts at: 2605 3073 3465 3492 3493 3780 2605 Size: 468 392 27 1 287 5483 Fragments arranged by size: 5483 468 392 287 27 1 EcoRI G'AATT_C Cuts at: 3967 4345 3967 Size: 378 6280 FseI GG_CCGG'CC Cuts at: 3207 3207 Size: 6658 FspI TGC'GCA Cuts at: 5164 5164 Size: 6658 HaeII r_GCGC'y Cuts at: 1678 2683 2999 3239 5805 1678 Size: 1005 316 240 2566 2531 Fragments arranged by size: 2566 2531 1005 316 240 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2067 2099 2927 2959 3676 Size: 32 135 32 495 32 828 32 717 Cuts at: 3676 3708 5299 5331 5373 5405 1373 Size: 32 1591 32 42 32 2626 Fragments arranged by size: 2626 1591 828 717 495 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2347 4002 4248 669 Size: 988 281 409 1655 246 3079 Fragments arranged by size: 3079 1655 988 409 281 246 HindIII A'AGCT_T Cuts at: 748 2135 748 Size: 1387 5271 HpaI GTT'AAC Cuts at: 4248 4248 Size: 6658 KasI G'GCGC_C Cuts at: 2995 3235 2995 Size: 240 6418 KpnI G_GTAC'C Cuts at: 3983 3983 Size: 6658 MfeI C'AATT_G Cuts at: 4257 4257 Size: 6658 MluI A'CGCG_T Cuts at: 6534 6534 Size: 6658 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2811 5772 5956 1305 Size: 1506 2961 184 2007 Fragments arranged by size: 2961 2007 1506 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2042 10 Size: 54 1425 343 210 4626 Fragments arranged by size: 4626 1425 343 210 54 MslI CAynn'nnrTG Cuts at: 518 1166 2793 4616 4975 5134 518 Size: 648 1627 1823 359 159 2042 Fragments arranged by size: 2042 1823 1627 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2404 2516 2866 2931 3332 3359 3422 4074 Size: 798 112 350 65 401 27 63 652 Cuts at: 4074 4751 5899 6144 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 798 677 652 401 350 245 112 65 63 27 NaeI GCC'GGC Cuts at: 3205 3205 Size: 6658 NarI GG'CG_CC Cuts at: 2996 3236 2996 Size: 240 6418 NcoI C'CATG_G Cuts at: 513 1065 2004 2018 3210 513 Size: 552 939 14 1192 3961 Fragments arranged by size: 3961 1192 939 552 14 NdeI CA'TA_TG Cuts at: 387 2110 387 Size: 1723 4935 NgoMIV G'CCGG_C Cuts at: 3203 3203 Size: 6658 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6658 NotI GC'GGCC_GC Cuts at: 4105 4105 Size: 6658 NspI r_CATG'y Cuts at: 1132 1229 3906 4016 5561 1132 Size: 97 2677 110 1545 2229 Fragments arranged by size: 2677 2229 1545 110 97 PasI CC'CwG_GG Cuts at: 1348 3489 1348 Size: 2141 4517 PciI A'CATG_T Cuts at: 5557 5557 Size: 6658 PflMI CCAn_nnn'nTGG Cuts at: 1108 2024 1108 Size: 916 5742 PmeI GTTT'AAAC Cuts at: 3962 3962 Size: 6658 PmlI CAC'GTG Cuts at: 2314 2314 Size: 6658 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4727 4759 6240 6272 4727 Size: 32 1481 32 5113 Fragments arranged by size: 5113 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3780 3780 Size: 6658 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6658 PsiI TTA'TAA Cuts at: 4228 4228 Size: 6658 PspOMI G'GGCC_C Cuts at: 3072 3492 3072 Size: 420 6238 PstI C_TGCA'G Cuts at: 830 2035 2096 2216 3265 3292 4010 830 Size: 1205 61 120 1049 27 718 3478 Fragments arranged by size: 3478 1205 1049 718 120 61 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6658 PvuII CAG'CTG Cuts at: 2516 2931 3332 3359 2516 Size: 415 401 27 5815 Fragments arranged by size: 5815 415 401 27 SacI G_AGCT'C Cuts at: 3846 3846 Size: 6658 SalI G'TCGA_C Cuts at: 1936 2345 4000 1936 Size: 409 1655 4594 Fragments arranged by size: 4594 1655 409 SanDI GG'GwC_CC Cuts at: 3780 3780 Size: 6658 SapI GCTCTTCn'nnn_ Cuts at: 2568 3834 2568 Size: 1266 5392 SbfI CC_TGCA'GG Cuts at: 2096 4010 2096 Size: 1914 4744 ScaI AGT'ACT Cuts at: 4906 6618 4906 Size: 1712 4946 SfcI C'TryA_G Cuts at: 826 1045 2031 2092 2212 3261 3288 4006 5141 Size: 219 986 61 120 1049 27 718 1135 Cuts at: 5141 5822 6013 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1049 986 718 681 219 191 120 61 27 SfoI GGC'GCC Cuts at: 2997 3237 2997 Size: 240 6418 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6658 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6658 SmaI CCC'GGG Cuts at: 1872 3245 3985 1872 Size: 1373 740 4545 Fragments arranged by size: 4545 1373 740 SmlI C'TyrA_G Cuts at: 820 1017 1943 2132 3707 4762 5663 5925 820 Size: 197 926 189 1575 1055 901 262 1553 Fragments arranged by size: 1575 1553 1055 926 901 262 197 189 SnaBI TAC'GTA Cuts at: 493 4449 493 Size: 3956 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6658 SphI G_CATG'C Cuts at: 1132 4016 1132 Size: 2884 3774 SspI AAT'ATT Cuts at: 5 52 4582 5 Size: 47 4530 2081 Fragments arranged by size: 4530 2081 47 StuI AGG'CCT Cuts at: 1517 2054 3197 1517 Size: 537 1143 4978 Fragments arranged by size: 4978 1143 537 StyI C'CwwG_G Cuts at: 513 1065 2004 2018 2879 3210 3852 4097 513 Size: 552 939 14 861 331 642 245 3074 Fragments arranged by size: 3074 939 861 642 552 331 245 14 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2254 3782 4685 4702 4855 5040 5485 Size: 1255 674 1528 903 17 153 185 445 Cuts at: 5485 325 Size: 1498 Fragments arranged by size: 1528 1498 1255 903 674 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2103 4904 6616 Size: 274 80 33 51 1443 126 2801 1712 Cuts at: 6616 96 Size: 138 Fragments arranged by size: 2801 1712 1443 274 138 126 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6618 6650 6618 Size: 32 6626 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6658 XbaI T'CTAG_A Cuts at: 3994 3994 Size: 6658 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6658 XmaI C'CCGG_G Cuts at: 1870 3243 3983 1870 Size: 1373 740 4545 Fragments arranged by size: 4545 1373 740 XmnI GAAnn'nnTTC Cuts at: 4787 4787 Size: 6658 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4466 276 Size: 53 83 186 936 2932 2468 Fragments arranged by size: 2932 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FspAI NruI NsiI PacI PfoI PspXI PsrI RsrII SacII SexAI SfiI SrfI SwaI XcmI