Restriction map for
pFN21AB8355
(Linear) (Six-base) MAPSORT of: KIBB8355.seq Check: 5370 from: 1 to: 1953
KIBB8355.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1748 1953
Size: 1748 205
AccI GT'mk_AC
Cuts at: 0 341 1953
Size: 341 1612
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1782 1953
Size: 1782 171
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 41 1953
Size: 41 1912
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1255 1287 1953
Size: 1255 32 666
Fragments arranged by size:
1255 666 32
ApaI G_GGCC'C
Cuts at: 0 1071 1491 1953
Size: 1071 420 462
Fragments arranged by size:
1071 462 420
AvaI C'yCGr_G
Cuts at: 0 1238 1503 1953
Size: 1238 265 450
Fragments arranged by size:
1238 450 265
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1499 1532 1785 1818 1953
Size: 1499 33 253 33 135
Fragments arranged by size:
1499 253 135 33 33
BanI G'GyrC_C
Cuts at: 0 385 484 990 1230 1530 1953
Size: 385 99 506 240 300 423
Fragments arranged by size:
506 423 385 300 240 99
BanII G_rGCy'C
Cuts at: 0 96 479 1018 1071 1145 1217 1484 1491
Size: 96 383 539 53 74 72 267 7
Cuts at: 1491 1664 1736 1841 1953
Size: 173 72 105 112
Fragments arranged by size:
539 383 267 173 112 105 96 74
72 72 53 7
BbeI G_GCGC'C
Cuts at: 0 994 1234 1953
Size: 994 240 719
Fragments arranged by size:
994 719 240
BbsI GAAGACnn'nnnn_
Cuts at: 0 377 505 1953
Size: 377 128 1448
Fragments arranged by size:
1448 377 128
BbvCI CC'TCA_GC
Cuts at: 0 1695 1953
Size: 1695 258
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1567 1601 1953
Size: 1567 34 352
Fragments arranged by size:
1567 352 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1189 1953
Size: 1189 764
BlpI GC'TnA_GC
Cuts at: 0 1052 1953
Size: 1052 901
Bme1580I G_kGCm'C
Cuts at: 0 487 1071 1491 1535 1953
Size: 487 584 420 44 418
Fragments arranged by size:
584 487 420 418 44
BmgBI CAC'GTC
Cuts at: 0 345 1953
Size: 345 1608
BmrI ACTGGGnnnn_n'
Cuts at: 0 267 1909 1953
Size: 267 1642 44
Fragments arranged by size:
1642 267 44
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 92 1101 1953
Size: 92 1009 852
Fragments arranged by size:
1009 852 92
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 411 1011 1456 1732 1865 1953
Size: 72 339 600 445 276 133 88
Fragments arranged by size:
600 445 339 276 133 88 72
Bpu10I CC'TnA_GC
Cuts at: 0 314 616 1616 1695 1953
Size: 314 302 1000 79 258
Fragments arranged by size:
1000 314 302 258 79
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 1723 1953
Size: 112 1611 230
Fragments arranged by size:
1611 230 112
BsaI GGTCTCn'nnnn_
Cuts at: 0 796 1126 1162 1750 1953
Size: 796 330 36 588 203
Fragments arranged by size:
796 588 330 203 36
BsaAI yAC'GTr
Cuts at: 0 246 309 1953
Size: 246 63 1644
Fragments arranged by size:
1644 246 63
BsaBI GATnn'nnATC
Cuts at: 0 1891 1953
Size: 1891 62
BsaHI Gr'CG_yC
Cuts at: 0 991 1231 1331 1953
Size: 991 240 100 622
Fragments arranged by size:
991 622 240 100
BsaWI w'CCGG_w
Cuts at: 0 980 1554 1953
Size: 980 574 399
Fragments arranged by size:
980 574 399
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1804 1834 1953
Size: 1804 30 119
Fragments arranged by size:
1804 119 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 598 601 676 718 808 850 1077 1180
Size: 598 3 75 42 90 42 227 103
Cuts at: 1180 1225 1720 1828 1831 1942 1945 1953
Size: 45 495 108 3 111 3 8
Fragments arranged by size:
598 495 227 111 108 103 90 75
45 42 42 8 3 3 3
BseYI C'CCAG_C
Cuts at: 0 4 857 1144 1901 1953
Size: 4 853 287 757 52
Fragments arranged by size:
853 757 287 52 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 1953
Size: 424 1529
BsiHKAI G_wGCw'C
Cuts at: 0 251 710 1841 1953
Size: 251 459 1131 112
Fragments arranged by size:
1131 459 251 112
Bsp1286I G_dGCh'C
Cuts at: 0 96 251 479 487 710 1018 1071 1145
Size: 96 155 228 8 223 308 53 74
Cuts at: 1145 1217 1484 1491 1535 1664 1736 1841 1953
Size: 72 267 7 44 129 72 105 112
Fragments arranged by size:
308 267 228 223 155 129 112 105
96 74 72 72 53 44 8 7
BspEI T'CCGG_A
Cuts at: 0 1554 1953
Size: 1554 399
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1748 1953
Size: 1748 205
BsrBI CCG'CTC
Cuts at: 0 591 633 648 672 687 1065 1953
Size: 591 42 15 24 15 378 888
Fragments arranged by size:
888 591 378 42 24 15 15
BsrFI r'CCGG_y
Cuts at: 0 1198 1953
Size: 1198 755
BsrGI T'GTAC_A
Cuts at: 0 98 1953
Size: 98 1855
BssSI C'ACGA_G
Cuts at: 0 79 1953
Size: 79 1874
BstAPI GCAn_nnn'nTGC
Cuts at: 0 447 1953
Size: 447 1506
BstEII G'GTnAC_C
Cuts at: 0 778 1080 1515 1953
Size: 778 302 435 438
Fragments arranged by size:
778 438 435 302
BstXI CCAn_nnnn'nTGG
Cuts at: 0 309 366 1953
Size: 309 57 1587
Fragments arranged by size:
1587 309 57
Bsu36I CC'TnA_GG
Cuts at: 0 1187 1220 1953
Size: 1187 33 733
Fragments arranged by size:
1187 733 33
BtgI C'CryG_G
Cuts at: 0 13 479 1205 1310 1334 1953
Size: 13 466 726 105 24 619
Fragments arranged by size:
726 619 466 105 24 13
BtsI GCAGTG_nn'
Cuts at: 0 216 1102 1373 1379 1561 1953
Size: 216 886 271 6 182 392
Fragments arranged by size:
886 392 271 216 182 6
EaeI y'GGCC_r
Cuts at: 0 35 1953
Size: 35 1918
EarI CTCTTCn'nnn_
Cuts at: 0 563 734 794 1829 1919 1953
Size: 563 171 60 1035 90 34
Fragments arranged by size:
1035 563 171 90 60 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1844 1953
Size: 1844 109
EcoICRI GAG'CTC
Cuts at: 0 1839 1953
Size: 1839 114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 411 1011 1456 1732 1782 1865 1953
Size: 72 339 600 445 276 50 83 88
Fragments arranged by size:
600 445 339 276 88 83 72 50
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1075 1525 1953
Size: 1075 450 428
Fragments arranged by size:
1075 450 428
EcoO109I rG'GnC_Cy
Cuts at: 0 600 1068 1460 1487 1488 1775 1953
Size: 600 468 392 27 1 287 178
Fragments arranged by size:
600 468 392 287 178 27 1
FseI GG_CCGG'CC
Cuts at: 0 1202 1953
Size: 1202 751
HaeII r_GCGC'y
Cuts at: 0 678 994 1234 1953
Size: 678 316 240 719
Fragments arranged by size:
719 678 316 240
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 62 94 922 954 1671 1703 1953
Size: 62 32 828 32 717 32 250
Fragments arranged by size:
828 717 250 62 32 32 32
HincII GTy'rAC
Cuts at: 0 342 1953
Size: 342 1611
HindIII A'AGCT_T
Cuts at: 0 130 1953
Size: 130 1823
KasI G'GCGC_C
Cuts at: 0 990 1230 1953
Size: 990 240 723
Fragments arranged by size:
990 723 240
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 806 1953
Size: 806 1147
MscI TGG'CCA
Cuts at: 0 37 1953
Size: 37 1916
MslI CAynn'nnrTG
Cuts at: 0 788 1953
Size: 788 1165
MspA1I CmG'CkG
Cuts at: 0 399 511 861 926 1327 1354 1417 1953
Size: 399 112 350 65 401 27 63 536
Fragments arranged by size:
536 401 399 350 112 65 63 27
NaeI GCC'GGC
Cuts at: 0 1200 1953
Size: 1200 753
NarI GG'CG_CC
Cuts at: 0 991 1231 1953
Size: 991 240 722
Fragments arranged by size:
991 722 240
NcoI C'CATG_G
Cuts at: 0 13 1205 1953
Size: 13 1192 748
Fragments arranged by size:
1192 748 13
NdeI CA'TA_TG
Cuts at: 0 105 1953
Size: 105 1848
NgoMIV G'CCGG_C
Cuts at: 0 1198 1953
Size: 1198 755
NspI r_CATG'y
Cuts at: 0 1901 1953
Size: 1901 52
PasI CC'CwG_GG
Cuts at: 0 1484 1953
Size: 1484 469
PflMI CCAn_nnn'nTGG
Cuts at: 0 19 1953
Size: 19 1934
PmlI CAC'GTG
Cuts at: 0 309 1953
Size: 309 1644
PpuMI rG'GwC_Cy
Cuts at: 0 1775 1953
Size: 1775 178
PspOMI G'GGCC_C
Cuts at: 0 1067 1487 1953
Size: 1067 420 466
Fragments arranged by size:
1067 466 420
PstI C_TGCA'G
Cuts at: 0 30 91 211 1260 1287 1953
Size: 30 61 120 1049 27 666
Fragments arranged by size:
1049 666 120 61 30 27
PvuII CAG'CTG
Cuts at: 0 511 926 1327 1354 1953
Size: 511 415 401 27 599
Fragments arranged by size:
599 511 415 401 27
SacI G_AGCT'C
Cuts at: 0 1841 1953
Size: 1841 112
SalI G'TCGA_C
Cuts at: 0 340 1953
Size: 340 1613
SanDI GG'GwC_CC
Cuts at: 0 1775 1953
Size: 1775 178
SapI GCTCTTCn'nnn_
Cuts at: 0 563 1829 1953
Size: 563 1266 124
Fragments arranged by size:
1266 563 124
SbfI CC_TGCA'GG
Cuts at: 0 91 1953
Size: 91 1862
SfcI C'TryA_G
Cuts at: 0 26 87 207 1256 1283 1953
Size: 26 61 120 1049 27 670
Fragments arranged by size:
1049 670 120 61 27 26
SfoI GGC'GCC
Cuts at: 0 992 1232 1953
Size: 992 240 721
Fragments arranged by size:
992 721 240
SmaI CCC'GGG
Cuts at: 0 1240 1953
Size: 1240 713
SmlI C'TyrA_G
Cuts at: 0 127 1702 1953
Size: 127 1575 251
Fragments arranged by size:
1575 251 127
StuI AGG'CCT
Cuts at: 0 49 1192 1953
Size: 49 1143 761
Fragments arranged by size:
1143 761 49
StyI C'CwwG_G
Cuts at: 0 13 874 1205 1847 1953
Size: 13 861 331 642 106
Fragments arranged by size:
861 642 331 106 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 249 1777 1953
Size: 249 1528 176
Fragments arranged by size:
1528 249 176
TatI w'GTAC_w
Cuts at: 0 98 1953
Size: 98 1855
XmaI C'CCGG_G
Cuts at: 0 1238 1953
Size: 1238 715
Enzymes that do cut:
AarI AccI AcuI AlfI AloI ApaI AvaI BaeI
BanI BanII BbeI BbsI BbvCI BcgI BglI BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BssSI
BstAPI BstEII BstXI Bsu36I BtgI BtsI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FseI HaeII Hin4I
HincII HindIII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PasI PflMI PmlI
PpuMI PspOMI PstI PvuII SacI SalI SanDI SapI
SbfI SfcI SfoI SmaI SmlI StuI StyI TaqII
TatI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII
BamHI BciVI BclI BfrBI BglII BmtI BsiEI BsiWI
BsmI BsmBI BspHI BsrDI BssHII BstBI BstYI BstZ17I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoRI
EcoRV FalI FspI FspAI HpaI KpnI MfeI MluI
NheI NotI NruI NsiI PacI PciI PfoI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TstI Tth111I XbaI XcmI XhoI
XmnI ZraI