(Circular) (Six-base) MAPSORT of: pFN21AB8354.seq Check: 5037 from: 1 to: 6076 pFN21AB8354 6076 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3886 278 Size: 53 83 186 936 2350 2468 Fragments arranged by size: 2468 2350 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2835 3419 1937 Size: 898 584 4594 Fragments arranged by size: 4594 898 584 Acc65I G'GTAC_C Cuts at: 3397 3397 Size: 6076 AclI AA'CG_TT Cuts at: 1563 4203 4576 1563 Size: 2640 373 3063 Fragments arranged by size: 3063 2640 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3008 3288 4139 5523 5791 1632 Size: 1376 280 851 1384 268 1917 Fragments arranged by size: 1917 1384 1376 851 280 268 AflII C'TTAA_G Cuts at: 820 1017 2744 820 Size: 197 1727 4152 Fragments arranged by size: 4152 1727 197 AflIII A'CryG_T Cuts at: 1203 3237 4975 5952 1203 Size: 2034 1738 977 1327 Fragments arranged by size: 2034 1738 1327 977 AgeI A'CCGG_T Cuts at: 4907 4907 Size: 6076 AhdI GACnn_n'nnGTC Cuts at: 4805 4805 Size: 6076 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6076 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2873 1184 Size: 1689 4387 AlwNI CAG_nnn'CTG Cuts at: 2054 3819 5391 2054 Size: 1765 1572 2739 Fragments arranged by size: 2739 1765 1572 ApaLI G'TGCA_C Cuts at: 4133 5289 4133 Size: 1156 4920 ApoI r'AATT_y Cuts at: 1493 2367 2699 3385 3611 3763 5872 1493 Size: 874 332 686 226 152 2109 1697 Fragments arranged by size: 2109 1697 874 686 332 226 152 AseI AT'TA_AT Cuts at: 160 1032 4630 160 Size: 872 3598 1606 Fragments arranged by size: 3598 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6076 AvaI C'yCGr_G Cuts at: 1870 1943 3401 1870 Size: 73 1458 4545 Fragments arranged by size: 4545 1458 73 BamHI G'GATC_C Cuts at: 3406 3773 3406 Size: 367 5709 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2021 3397 4852 618 Size: 325 217 627 234 1376 1455 1842 Fragments arranged by size: 1842 1455 1376 627 325 234 217 BanII G_rGCy'C Cuts at: 1391 2406 3395 1391 Size: 1015 989 4072 Fragments arranged by size: 4072 1015 989 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2592 3305 928 Size: 971 693 713 3699 Fragments arranged by size: 3699 971 713 693 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2328 2362 4267 4301 1903 Size: 34 391 34 1905 34 3678 Fragments arranged by size: 3678 1905 391 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2776 3968 5178 2776 Size: 1192 1210 3674 Fragments arranged by size: 3674 1210 1192 BclI T'GATC_A Cuts at: 1548 1548 Size: 6076 BfrBI ATG'CAT Cuts at: 2339 2339 Size: 6076 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4687 136 Size: 107 122 71 631 605 3015 1525 Fragments arranged by size: 3015 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3174 6071 3174 Size: 2897 3179 BlpI GC'TnA_GC Cuts at: 2061 2721 3493 2061 Size: 660 772 4644 Fragments arranged by size: 4644 772 660 Bme1580I G_kGCm'C Cuts at: 1790 4137 5293 1790 Size: 2347 1156 2573 Fragments arranged by size: 2573 2347 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6076 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3807 4765 442 Size: 416 53 83 399 24 2390 958 1753 Fragments arranged by size: 2390 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6076 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2642 4736 1239 Size: 1403 2094 2579 Fragments arranged by size: 2579 2094 1403 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4201 5066 5364 4201 Size: 865 298 4913 Fragments arranged by size: 4913 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4739 882 Size: 617 3240 2219 Fragments arranged by size: 3240 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3373 3867 493 Size: 711 2169 494 2702 Fragments arranged by size: 2702 2169 711 494 BsaBI GATnn'nnATC Cuts at: 6070 6070 Size: 6076 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3883 4265 4900 275 Size: 53 83 186 936 2350 382 635 1451 Fragments arranged by size: 2350 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2451 4509 4907 5181 5328 5739 2451 Size: 2058 398 274 147 411 2788 Fragments arranged by size: 2788 2058 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2145 2175 2490 2520 1178 Size: 30 165 30 742 30 315 30 4734 Fragments arranged by size: 4734 742 315 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6076 BseYI C'CCAG_C Cuts at: 3084 5279 5775 3084 Size: 2195 496 3385 Fragments arranged by size: 3385 2195 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5503 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3526 4287 5315 1540 Size: 275 186 1525 761 1028 2301 Fragments arranged by size: 2301 1525 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2406 3207 4137 4222 5293 2406 Size: 801 930 85 1071 3189 Fragments arranged by size: 3189 1071 930 801 85 BsmI GAATG_Cn' Cuts at: 2383 2564 3023 3587 3680 2383 Size: 181 459 564 93 4779 Fragments arranged by size: 4779 564 459 181 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2406 3207 3395 4137 4222 5293 1391 Size: 399 616 801 188 742 85 1071 2174 Fragments arranged by size: 2174 1071 801 742 616 399 188 85 BspEI T'CCGG_A Cuts at: 2451 5739 2451 Size: 3288 2788 BspHI T'CATG_A Cuts at: 2215 3093 3965 5695 2215 Size: 878 872 1730 2596 Fragments arranged by size: 2596 1730 878 872 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3431 844 Size: 2587 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3214 3963 1394 Size: 429 1391 749 3507 Fragments arranged by size: 3507 1391 749 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3294 3351 4571 4745 65 Size: 1183 2046 57 1220 174 1396 Fragments arranged by size: 2046 1396 1220 1183 174 57 BsrFI r'CCGG_y Cuts at: 1700 1810 4720 4907 1700 Size: 110 2910 187 2869 Fragments arranged by size: 2910 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6076 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6076 BssSI C'ACGA_G Cuts at: 4136 5148 4136 Size: 1012 5064 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6076 BstBI TT'CG_AA Cuts at: 2754 4880 2754 Size: 2126 3950 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6076 BstYI r'GATC_y Cuts at: 1972 3174 3406 3773 4158 4175 5627 5713 5725 Size: 1202 232 367 385 17 1452 86 12 Cuts at: 5725 6071 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1202 385 367 346 232 86 17 12 BstZ17I GTA'TAC Cuts at: 2836 2836 Size: 6076 Bsu36I CC'TnA_GG Cuts at: 3363 3363 Size: 6076 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5524 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5337 Fragments arranged by size: 5337 683 56 BtsI GCAGTG_nn' Cuts at: 3592 4386 4406 3592 Size: 794 20 5262 Fragments arranged by size: 5262 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6076 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6041 DraI TTT'AAA Cuts at: 3380 3380 Size: 6076 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5083 809 Size: 507 3767 1802 Fragments arranged by size: 3767 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2973 3523 4412 Size: 54 1425 50 275 18 1143 550 889 Cuts at: 4412 6041 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1143 889 550 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3523 1537 Size: 275 1711 4090 Fragments arranged by size: 4090 1711 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3267 3348 4006 1353 Size: 1914 81 658 3423 Fragments arranged by size: 3423 1914 658 81 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4663 5037 5183 387 Size: 1064 138 3074 374 146 1280 Fragments arranged by size: 3074 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2404 2404 Size: 6076 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2642 3008 3288 4139 4736 5523 5791 Size: 393 1010 366 280 851 597 787 268 Cuts at: 5791 1239 Size: 1524 Fragments arranged by size: 1524 1010 851 787 597 393 366 280 268 EcoO109I rG'GnC_Cy Cuts at: 3031 3031 Size: 6076 EcoRI G'AATT_C Cuts at: 3385 3763 3385 Size: 378 5698 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2759 3139 2759 Size: 380 5696 FspI TGC'GCA Cuts at: 4582 4582 Size: 6076 HaeII r_GCGC'y Cuts at: 1678 5223 1678 Size: 3545 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2066 2098 2433 2465 3092 Size: 32 135 32 494 32 335 32 627 Cuts at: 3092 3124 3159 3191 4717 4749 4791 4823 1373 Size: 32 35 32 1526 32 42 32 2626 Fragments arranged by size: 2626 1526 627 494 335 135 42 35 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3420 3666 669 Size: 988 281 1482 246 3079 Fragments arranged by size: 3079 1482 988 281 246 HindIII A'AGCT_T Cuts at: 748 2934 2990 748 Size: 2186 56 3834 Fragments arranged by size: 3834 2186 56 HpaI GTT'AAC Cuts at: 3666 3666 Size: 6076 KpnI G_GTAC'C Cuts at: 3401 3401 Size: 6076 MfeI C'AATT_G Cuts at: 2601 3675 2601 Size: 1074 5002 MluI A'CGCG_T Cuts at: 5952 5952 Size: 6076 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5190 5374 1305 Size: 3885 184 2007 Fragments arranged by size: 3885 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2975 10 Size: 54 1425 343 1143 3111 Fragments arranged by size: 3111 1425 1143 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2282 4034 4393 4552 518 Size: 648 1116 1752 359 159 2042 Fragments arranged by size: 2042 1752 1116 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2529 2635 3088 3492 4169 5317 5562 1606 Size: 923 106 453 404 677 1148 245 2120 Fragments arranged by size: 2120 1148 923 677 453 404 245 106 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5524 NdeI CA'TA_TG Cuts at: 387 2341 387 Size: 1954 4122 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6076 NotI GC'GGCC_GC Cuts at: 3523 3523 Size: 6076 NsiI A_TGCA'T Cuts at: 2341 2341 Size: 6076 NspI r_CATG'y Cuts at: 1132 1229 2860 3241 3434 4979 1132 Size: 97 1631 381 193 1545 2229 Fragments arranged by size: 2229 1631 1545 381 193 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6076 PciI A'CATG_T Cuts at: 3237 4975 3237 Size: 1738 4338 PflMI CCAn_nnn'nTGG Cuts at: 1108 2672 1108 Size: 1564 4512 PmeI GTTT'AAAC Cuts at: 3380 3380 Size: 6076 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4145 4177 5658 5690 4145 Size: 32 1481 32 4531 Fragments arranged by size: 4531 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6076 PsiI TTA'TAA Cuts at: 3646 3646 Size: 6076 PstI C_TGCA'G Cuts at: 830 2555 3174 3428 830 Size: 1725 619 254 3478 Fragments arranged by size: 3478 1725 619 254 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6076 PvuII CAG'CTG Cuts at: 3088 3088 Size: 6076 SacI G_AGCT'C Cuts at: 2406 2406 Size: 6076 SalI G'TCGA_C Cuts at: 1936 3418 1936 Size: 1482 4594 SbfI CC_TGCA'GG Cuts at: 3428 3428 Size: 6076 ScaI AGT'ACT Cuts at: 4324 6036 4324 Size: 1712 4364 SfcI C'TryA_G Cuts at: 826 1045 2551 3170 3424 4559 5240 5431 826 Size: 219 1506 619 254 1135 681 191 1471 Fragments arranged by size: 1506 1471 1135 681 619 254 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6076 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6076 SmaI CCC'GGG Cuts at: 1872 3403 1872 Size: 1531 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2744 4180 5081 5343 820 Size: 197 926 801 1436 901 262 1553 Fragments arranged by size: 1553 1436 926 901 801 262 197 SnaBI TAC'GTA Cuts at: 493 3867 493 Size: 3374 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6076 SphI G_CATG'C Cuts at: 1132 3434 1132 Size: 2302 3774 SspI AAT'ATT Cuts at: 5 52 4000 5 Size: 47 3948 2081 Fragments arranged by size: 3948 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6076 StyI C'CwwG_G Cuts at: 513 1065 3034 3114 3198 3267 3515 513 Size: 552 1969 80 84 69 248 3074 Fragments arranged by size: 3074 1969 552 248 84 80 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4103 4120 4273 4458 4903 325 Size: 1255 2523 17 153 185 445 1498 Fragments arranged by size: 2523 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4322 6034 96 Size: 274 80 33 51 1443 2345 1712 138 Fragments arranged by size: 2345 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6036 6068 6036 Size: 32 6044 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6076 XbaI T'CTAG_A Cuts at: 3412 3412 Size: 6076 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2794 3286 2794 Size: 492 5584 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6076 XmaI C'CCGG_G Cuts at: 1870 3401 1870 Size: 1531 4545 XmnI GAAnn'nnTTC Cuts at: 2946 4205 2946 Size: 1259 4817 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3884 276 Size: 53 83 186 936 2350 2468 Fragments arranged by size: 2468 2350 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI