(Circular) (Six-base) MAPSORT of: pFN21AB8345.seq Check: 4063 from: 1 to: 6589 pFN21AB8345 6589 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4399 278 Size: 53 83 186 936 2863 2468 Fragments arranged by size: 2863 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3932 1937 Size: 1995 4594 Acc65I G'GTAC_C Cuts at: 3910 3910 Size: 6589 AclI AA'CG_TT Cuts at: 1563 2813 4716 5089 1563 Size: 1250 1903 373 3063 Fragments arranged by size: 3063 1903 1250 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2950 3370 3450 3813 4652 6036 6304 1632 Size: 1318 420 80 363 839 1384 268 1917 Fragments arranged by size: 1917 1384 1318 839 420 363 268 80 AflII C'TTAA_G Cuts at: 820 1017 3739 820 Size: 197 2722 3670 Fragments arranged by size: 3670 2722 197 AflIII A'CryG_T Cuts at: 1203 2442 3412 3580 3748 5488 6465 1203 Size: 1239 970 168 168 1740 977 1327 Fragments arranged by size: 1740 1327 1239 977 970 168 168 AgeI A'CCGG_T Cuts at: 5420 5420 Size: 6589 AhdI GACnn_n'nnGTC Cuts at: 5318 5318 Size: 6589 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6589 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6589 AlwNI CAG_nnn'CTG Cuts at: 2122 4332 5904 2122 Size: 2210 1572 2807 Fragments arranged by size: 2807 2210 1572 ApaLI G'TGCA_C Cuts at: 3254 4646 5802 3254 Size: 1392 1156 4041 Fragments arranged by size: 4041 1392 1156 ApoI r'AATT_y Cuts at: 1493 2262 2409 3812 3898 4124 4276 6385 1493 Size: 769 147 1403 86 226 152 2109 1697 Fragments arranged by size: 2109 1697 1403 769 226 152 147 86 AseI AT'TA_AT Cuts at: 160 1032 5143 160 Size: 872 4111 1606 Fragments arranged by size: 4111 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6589 AvaI C'yCGr_G Cuts at: 1870 1943 3914 1870 Size: 73 1971 4545 Fragments arranged by size: 4545 1971 73 BamHI G'GATC_C Cuts at: 3919 4286 3919 Size: 367 6222 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3910 5365 618 Size: 325 217 627 2123 1455 1842 Fragments arranged by size: 2123 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3908 1391 Size: 2517 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2449 2827 2911 2995 3079 3134 3163 Size: 971 550 378 84 84 84 55 29 Cuts at: 3163 3438 3628 928 Size: 275 190 3889 Fragments arranged by size: 3889 971 550 378 275 190 84 84 84 55 29 BbvCI CC'TCA_GC Cuts at: 2375 2375 Size: 6589 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4780 4814 1903 Size: 34 2843 34 3678 Fragments arranged by size: 3678 2843 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4481 5691 4481 Size: 1210 5379 BclI T'GATC_A Cuts at: 1548 2356 3858 1548 Size: 808 1502 4279 Fragments arranged by size: 4279 1502 808 BfrBI ATG'CAT Cuts at: 2464 2572 2464 Size: 108 6481 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5200 136 Size: 107 122 71 631 605 3528 1525 Fragments arranged by size: 3528 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3853 6584 3853 Size: 2731 3858 BlpI GC'TnA_GC Cuts at: 4006 4006 Size: 6589 Bme1580I G_kGCm'C Cuts at: 1790 3258 4650 5806 1790 Size: 1468 1392 1156 2573 Fragments arranged by size: 2573 1468 1392 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6589 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3139 4320 5278 Size: 416 53 83 399 24 1722 1181 958 Cuts at: 5278 442 Size: 1753 Fragments arranged by size: 1753 1722 1181 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6589 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2390 2390 Size: 6589 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2069 2126 2439 2946 3030 3044 3114 3282 Size: 830 57 313 507 84 14 70 168 Cuts at: 3282 3702 5249 1239 Size: 420 1547 2579 Fragments arranged by size: 2579 1547 830 507 420 313 168 84 70 57 14 Bpu10I CC'TnA_GC Cuts at: 2375 2375 Size: 6589 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2465 2710 4714 5579 5877 2465 Size: 245 2004 865 298 3177 Fragments arranged by size: 3177 2004 865 298 245 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2102 2590 5252 882 Size: 617 603 488 2662 2219 Fragments arranged by size: 2662 2219 617 603 488 BsaAI yAC'GTr Cuts at: 493 1204 2445 4380 493 Size: 711 1241 1935 2702 Fragments arranged by size: 2702 1935 1241 711 BsaBI GATnn'nnATC Cuts at: 6583 6583 Size: 6589 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4396 4778 5413 275 Size: 53 83 186 936 2863 382 635 1451 Fragments arranged by size: 2863 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2756 5022 5420 5694 5841 6252 2756 Size: 2266 398 274 147 411 3093 Fragments arranged by size: 3093 2266 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3215 3245 1178 Size: 30 165 30 1812 30 4522 Fragments arranged by size: 4522 1812 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2066 3220 1186 Size: 880 1154 4555 Fragments arranged by size: 4555 1154 880 BseYI C'CCAG_C Cuts at: 5792 6288 5792 Size: 496 6093 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3659 3841 1163 Size: 573 1923 182 3911 Fragments arranged by size: 3911 1923 573 182 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4039 4800 5828 1540 Size: 275 186 2038 761 1028 2301 Fragments arranged by size: 2301 2038 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3258 4650 4735 5806 3258 Size: 1392 85 1071 4041 Fragments arranged by size: 4041 1392 1071 85 BsiWI C'GTAC_G Cuts at: 2445 2445 Size: 6589 BsmI GAATG_Cn' Cuts at: 2574 3032 3116 3200 3215 3426 3452 3719 4100 Size: 458 84 84 15 211 26 267 381 Cuts at: 4100 4193 2574 Size: 93 4970 Fragments arranged by size: 4970 458 381 267 211 93 84 84 26 15 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3258 3908 4650 4735 5806 1391 Size: 399 1468 650 742 85 1071 2174 Fragments arranged by size: 2174 1468 1071 742 650 399 85 BspEI T'CCGG_A Cuts at: 6252 6252 Size: 6589 BspHI T'CATG_A Cuts at: 4478 6208 4478 Size: 1730 4859 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3944 844 Size: 3100 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4476 1394 Size: 429 2653 3507 Fragments arranged by size: 3507 2653 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2705 3293 5084 5258 65 Size: 1183 1457 588 1791 174 1396 Fragments arranged by size: 1791 1457 1396 1183 588 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5233 5420 1700 Size: 110 3423 187 2869 Fragments arranged by size: 3423 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2400 96 Size: 2304 4285 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6589 BssSI C'ACGA_G Cuts at: 4649 5661 4649 Size: 1012 5577 BstAPI GCAn_nnn'nTGC Cuts at: 794 3204 794 Size: 2410 4179 BstBI TT'CG_AA Cuts at: 2818 2902 2986 3070 3154 5393 2818 Size: 84 84 84 84 2239 4014 Fragments arranged by size: 4014 2239 84 84 84 84 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6589 BstXI CCAn_nnnn'nTGG Cuts at: 2534 2534 Size: 6589 BstYI r'GATC_y Cuts at: 1972 3853 3919 4286 4671 4688 6140 6226 6238 Size: 1881 66 367 385 17 1452 86 12 Cuts at: 6238 6584 1972 Size: 346 1977 Fragments arranged by size: 1977 1881 1452 385 367 346 86 66 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5098 Fragments arranged by size: 5098 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2450 487 Size: 683 56 1224 4626 Fragments arranged by size: 4626 1224 683 56 BtsI GCAGTG_nn' Cuts at: 2670 3377 3545 3829 4105 4899 4919 2670 Size: 707 168 284 276 794 20 4340 Fragments arranged by size: 4340 794 707 284 276 168 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6589 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3019 3054 3103 3138 3271 3306 3439 Size: 35 2455 35 49 35 133 35 133 Cuts at: 3439 3474 3523 3558 529 Size: 35 49 35 3560 Fragments arranged by size: 3560 2455 133 133 49 49 35 35 35 35 35 35 DraI TTT'AAA Cuts at: 3893 3893 Size: 6589 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2015 5596 809 Size: 507 699 3581 1802 Fragments arranged by size: 3581 1802 699 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3644 4036 4925 Size: 54 1425 50 275 18 1814 392 889 Cuts at: 4925 6554 8 Size: 1629 43 Fragments arranged by size: 1814 1629 1425 889 392 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4036 1537 Size: 275 2224 4090 Fragments arranged by size: 4090 2224 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4519 1353 Size: 3166 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5176 5550 5696 387 Size: 1064 138 3587 374 146 1280 Fragments arranged by size: 3587 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2126 2439 2946 2950 3030 3044 Size: 393 437 57 313 507 4 80 14 Cuts at: 3044 3114 3282 3370 3450 3702 3813 4652 5249 Size: 70 168 88 80 252 111 839 597 Cuts at: 5249 6036 6304 1239 Size: 787 268 1524 Fragments arranged by size: 1524 839 787 597 507 437 393 313 268 252 168 111 88 80 80 70 57 14 4 EcoO109I rG'GnC_Cy Cuts at: 2083 2083 Size: 6589 EcoRI G'AATT_C Cuts at: 3812 3898 4276 3812 Size: 86 378 6125 Fragments arranged by size: 6125 378 86 FspI TGC'GCA Cuts at: 5095 5095 Size: 6589 HaeII r_GCGC'y Cuts at: 1678 5736 1678 Size: 4058 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2374 2406 3843 3875 5230 Size: 32 135 32 802 32 1437 32 1355 Cuts at: 5230 5262 5304 5336 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1437 1355 802 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3933 4179 669 Size: 988 281 1995 246 3079 Fragments arranged by size: 3079 1995 988 281 246 HindIII A'AGCT_T Cuts at: 748 2793 3465 3549 748 Size: 2045 672 84 3788 Fragments arranged by size: 3788 2045 672 84 HpaI GTT'AAC Cuts at: 4179 4179 Size: 6589 KpnI G_GTAC'C Cuts at: 3914 3914 Size: 6589 MfeI C'AATT_G Cuts at: 3304 3724 4188 3304 Size: 420 464 5705 Fragments arranged by size: 5705 464 420 MluI A'CGCG_T Cuts at: 6465 6465 Size: 6589 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2313 5703 5887 1305 Size: 1008 3390 184 2007 Fragments arranged by size: 3390 2007 1008 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3646 10 Size: 54 1425 343 1814 2953 Fragments arranged by size: 2953 1814 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2521 2773 2857 2941 3251 4547 4906 Size: 648 1355 252 84 84 310 1296 359 Cuts at: 4906 5065 518 Size: 159 2042 Fragments arranged by size: 2042 1355 1296 648 359 310 252 159 84 84 MspA1I CmG'CkG Cuts at: 1606 4005 4682 5830 6075 1606 Size: 2399 677 1148 245 2120 Fragments arranged by size: 2399 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5098 Fragments arranged by size: 5098 939 552 NdeI CA'TA_TG Cuts at: 387 2910 3330 3498 3666 387 Size: 2523 420 168 168 3310 Fragments arranged by size: 3310 2523 420 168 168 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6589 NotI GC'GGCC_GC Cuts at: 4036 4036 Size: 6589 NsiI A_TGCA'T Cuts at: 2466 2574 2466 Size: 108 6481 NspI r_CATG'y Cuts at: 1132 1229 2576 2660 3416 3584 3752 3947 5492 Size: 97 1347 84 756 168 168 195 1545 Cuts at: 5492 1132 Size: 2229 Fragments arranged by size: 2229 1545 1347 756 195 168 168 97 84 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6589 PciI A'CATG_T Cuts at: 3412 3580 3748 5488 3412 Size: 168 168 1740 4513 Fragments arranged by size: 4513 1740 168 168 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6589 PmeI GTTT'AAAC Cuts at: 3893 3893 Size: 6589 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2111 2143 4658 4690 6171 6203 2111 Size: 32 2515 32 1481 32 2497 Fragments arranged by size: 2515 2497 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2083 2083 Size: 6589 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6589 PsiI TTA'TAA Cuts at: 4159 4159 Size: 6589 PstI C_TGCA'G Cuts at: 830 3941 830 Size: 3111 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6589 SalI G'TCGA_C Cuts at: 1936 3931 1936 Size: 1995 4594 SbfI CC_TGCA'GG Cuts at: 3941 3941 Size: 6589 ScaI AGT'ACT Cuts at: 4837 6549 4837 Size: 1712 4877 SfcI C'TryA_G Cuts at: 826 1045 2089 2545 3597 3765 3937 5072 5753 Size: 219 1044 456 1052 168 172 1135 681 Cuts at: 5753 5944 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1052 1044 681 456 219 191 172 168 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6589 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6589 SmaI CCC'GGG Cuts at: 1872 3916 1872 Size: 2044 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2480 2725 3739 4693 5594 5856 Size: 197 926 537 245 1014 954 901 262 Cuts at: 5856 820 Size: 1553 Fragments arranged by size: 1553 1014 954 926 901 537 262 245 197 SnaBI TAC'GTA Cuts at: 493 4380 493 Size: 3887 2702 SpeI A'CTAG_T Cuts at: 152 2735 152 Size: 2583 4006 SphI G_CATG'C Cuts at: 1132 3947 1132 Size: 2815 3774 SspI AAT'ATT Cuts at: 5 52 4513 5 Size: 47 4461 2081 Fragments arranged by size: 4461 2081 47 StuI AGG'CCT Cuts at: 1517 2543 2963 1517 Size: 1026 420 5143 Fragments arranged by size: 5143 1026 420 StyI C'CwwG_G Cuts at: 513 1065 2004 2202 4028 513 Size: 552 939 198 1826 3074 Fragments arranged by size: 3074 1826 939 552 198 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4616 4633 4786 4971 5416 325 Size: 1255 3036 17 153 185 445 1498 Fragments arranged by size: 3036 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2400 4835 6547 Size: 274 80 33 51 1443 423 2435 1712 Cuts at: 6547 96 Size: 138 Fragments arranged by size: 2435 1712 1443 423 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3160 3192 3215 3247 6549 6581 3160 Size: 32 23 32 3302 32 3168 Fragments arranged by size: 3302 3168 32 32 32 23 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6589 XbaI T'CTAG_A Cuts at: 3925 3925 Size: 6589 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6589 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6589 XmaI C'CCGG_G Cuts at: 1870 3914 1870 Size: 2044 4545 XmnI GAAnn'nnTTC Cuts at: 2236 2795 3047 3084 3131 3215 3299 3383 3467 Size: 559 252 37 47 84 84 84 84 Cuts at: 3467 3551 3635 3719 4718 2236 Size: 84 84 84 999 4107 Fragments arranged by size: 4107 999 559 252 84 84 84 84 84 84 84 47 37 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4397 276 Size: 53 83 186 936 2863 2468 Fragments arranged by size: 2863 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI