(Circular) (Six-base) MAPSORT of: pFN21AB8344.seq Check: 6122 from: 1 to: 5608 pFN21AB8344 5608 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3418 278 Size: 53 83 186 936 1882 2468 Fragments arranged by size: 2468 1882 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2951 1937 Size: 1014 4594 Acc65I G'GTAC_C Cuts at: 2929 2929 Size: 5608 AclI AA'CG_TT Cuts at: 1563 3735 4108 1563 Size: 2172 373 3063 Fragments arranged by size: 3063 2172 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2387 3671 5055 5323 1632 Size: 755 1284 1384 268 1917 Fragments arranged by size: 1917 1384 1284 755 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5411 AflIII A'CryG_T Cuts at: 1203 2873 4507 5484 1203 Size: 1670 1634 977 1327 Fragments arranged by size: 1670 1634 1327 977 AgeI A'CCGG_T Cuts at: 4439 4439 Size: 5608 AhdI GACnn_n'nnGTC Cuts at: 4337 4337 Size: 5608 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5608 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2144 1184 Size: 960 4648 AlwNI CAG_nnn'CTG Cuts at: 2141 3351 4923 2141 Size: 1210 1572 2826 Fragments arranged by size: 2826 1572 1210 ApaI G_GGCC'C Cuts at: 2472 2472 Size: 5608 ApaLI G'TGCA_C Cuts at: 3665 4821 3665 Size: 1156 4452 ApoI r'AATT_y Cuts at: 1493 2813 2917 3143 3295 5404 1493 Size: 1320 104 226 152 2109 1697 Fragments arranged by size: 2109 1697 1320 226 152 104 AseI AT'TA_AT Cuts at: 160 1032 4162 160 Size: 872 3130 1606 Fragments arranged by size: 3130 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5608 AvaI C'yCGr_G Cuts at: 1870 1943 2933 1870 Size: 73 990 4545 Fragments arranged by size: 4545 990 73 BamHI G'GATC_C Cuts at: 2938 3305 2938 Size: 367 5241 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2202 2672 2929 4384 618 Size: 325 217 627 415 470 257 1455 1842 Fragments arranged by size: 1842 1455 627 470 415 325 257 217 BanII G_rGCy'C Cuts at: 1391 2472 2663 2927 1391 Size: 1081 191 264 4072 Fragments arranged by size: 4072 1081 264 191 BbeI G_GCGC'C Cuts at: 2206 2676 2206 Size: 470 5138 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4637 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3799 3833 1903 Size: 34 1862 34 3678 Fragments arranged by size: 3678 1862 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3500 4710 3500 Size: 1210 4398 BclI T'GATC_A Cuts at: 1548 2190 2265 1548 Size: 642 75 4891 Fragments arranged by size: 4891 642 75 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4219 136 Size: 107 122 71 631 605 2547 1525 Fragments arranged by size: 2547 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2493 5603 2493 Size: 3110 2498 BlpI GC'TnA_GC Cuts at: 2142 3025 2142 Size: 883 4725 Bme1580I G_kGCm'C Cuts at: 1790 2113 2472 3669 4825 1790 Size: 323 359 1197 1156 2573 Fragments arranged by size: 2573 1197 1156 359 323 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5608 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3339 4297 442 Size: 416 53 83 399 24 1922 958 1753 Fragments arranged by size: 1922 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5608 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2321 2482 2792 4268 1239 Size: 1082 161 310 1476 2579 Fragments arranged by size: 2579 1476 1082 310 161 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3733 4598 4896 3733 Size: 865 298 4445 Fragments arranged by size: 4445 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4271 882 Size: 617 2772 2219 Fragments arranged by size: 2772 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2874 3399 493 Size: 711 1670 525 2702 Fragments arranged by size: 2702 1670 711 525 BsaBI GATnn'nnATC Cuts at: 5602 5602 Size: 5608 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2203 2673 3415 3797 Size: 53 83 186 936 670 470 742 382 Cuts at: 3797 4432 275 Size: 635 1451 Fragments arranged by size: 1451 936 742 670 635 470 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2424 4041 4439 4713 4860 5271 2424 Size: 1617 398 274 147 411 2761 Fragments arranged by size: 2761 1617 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2228 2258 1178 Size: 30 165 30 825 30 4528 Fragments arranged by size: 4528 825 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2252 2324 2327 1186 Size: 1066 72 3 4467 Fragments arranged by size: 4467 1066 72 3 BseYI C'CCAG_C Cuts at: 2029 2113 2632 2767 4811 5307 2029 Size: 84 519 135 2044 496 2330 Fragments arranged by size: 2330 2044 519 496 135 84 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5035 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3058 3819 4847 1540 Size: 275 186 1057 761 1028 2301 Fragments arranged by size: 2301 1057 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2663 3669 3754 4825 2663 Size: 1006 85 1071 3446 Fragments arranged by size: 3446 1071 1006 85 BsmI GAATG_Cn' Cuts at: 2577 2701 3119 3212 2577 Size: 124 418 93 4973 Fragments arranged by size: 4973 418 124 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2113 2472 2663 2927 3669 3754 4825 Size: 399 323 359 191 264 742 85 1071 Cuts at: 4825 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 359 323 264 191 85 BspEI T'CCGG_A Cuts at: 5271 5271 Size: 5608 BspHI T'CATG_A Cuts at: 3497 5227 3497 Size: 1730 3878 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2280 2473 2963 844 Size: 1436 193 490 3489 Fragments arranged by size: 3489 1436 490 193 BsrBI CCG'CTC Cuts at: 1394 1823 3495 1394 Size: 429 1672 3507 Fragments arranged by size: 3507 1672 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4103 4277 65 Size: 1183 2855 174 1396 Fragments arranged by size: 2855 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4252 4439 1700 Size: 110 2442 187 2869 Fragments arranged by size: 2869 2442 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5608 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5608 BssSI C'ACGA_G Cuts at: 2084 3668 4680 2084 Size: 1584 1012 3012 Fragments arranged by size: 3012 1584 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2613 794 Size: 1819 3789 BstBI TT'CG_AA Cuts at: 4412 4412 Size: 5608 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5608 BstXI CCAn_nnnn'nTGG Cuts at: 2816 2816 Size: 5608 BstYI r'GATC_y Cuts at: 1972 2493 2938 3305 3690 3707 5159 5245 5257 Size: 521 445 367 385 17 1452 86 12 Cuts at: 5257 5603 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 521 445 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5056 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4869 Fragments arranged by size: 4869 683 56 BtsI GCAGTG_nn' Cuts at: 2068 2249 3124 3918 3938 2068 Size: 181 875 794 20 3738 Fragments arranged by size: 3738 875 794 181 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5608 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5573 DraI TTT'AAA Cuts at: 2912 2912 Size: 5608 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2290 4615 809 Size: 507 974 2325 1802 Fragments arranged by size: 2325 1802 974 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3055 3944 5573 Size: 54 1425 50 275 18 1225 889 1629 Cuts at: 5573 8 Size: 43 Fragments arranged by size: 1629 1425 1225 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3055 1537 Size: 275 1243 4090 Fragments arranged by size: 4090 1243 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2301 2400 2436 3538 1353 Size: 948 99 36 1102 3423 Fragments arranged by size: 3423 1102 948 99 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4195 4569 4715 387 Size: 1064 138 2606 374 146 1280 Fragments arranged by size: 2606 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2661 2661 Size: 5608 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2321 2387 2482 2792 3671 4268 5055 Size: 393 689 66 95 310 879 597 787 Cuts at: 5055 5323 1239 Size: 268 1524 Fragments arranged by size: 1524 879 787 689 597 393 310 268 95 66 EcoO109I rG'GnC_Cy Cuts at: 2292 2468 2469 2292 Size: 176 1 5431 Fragments arranged by size: 5431 176 1 EcoRI G'AATT_C Cuts at: 2917 3295 2917 Size: 378 5230 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2857 2857 Size: 5608 FspI TGC'GCA Cuts at: 4114 4114 Size: 5608 HaeII r_GCGC'y Cuts at: 1678 2206 2676 4755 1678 Size: 528 470 2079 2531 Fragments arranged by size: 2531 2079 528 470 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4249 4281 4323 4355 1373 Size: 32 135 32 2677 32 42 32 2626 Fragments arranged by size: 2677 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2170 2952 3198 669 Size: 988 281 232 782 246 3079 Fragments arranged by size: 3079 988 782 281 246 232 HindIII A'AGCT_T Cuts at: 748 2799 748 Size: 2051 3557 HpaI GTT'AAC Cuts at: 2170 3198 2170 Size: 1028 4580 KasI G'GCGC_C Cuts at: 2202 2672 2202 Size: 470 5138 KpnI G_GTAC'C Cuts at: 2933 2933 Size: 5608 MfeI C'AATT_G Cuts at: 3207 3207 Size: 5608 MluI A'CGCG_T Cuts at: 5484 5484 Size: 5608 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2199 4722 4906 1305 Size: 894 2523 184 2007 Fragments arranged by size: 2523 2007 894 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3786 Fragments arranged by size: 3786 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2301 2565 3566 3925 4084 518 Size: 648 1135 264 1001 359 159 2042 Fragments arranged by size: 2042 1135 1001 648 359 264 159 MspA1I CmG'CkG Cuts at: 1606 2636 2767 3024 3701 4849 5094 1606 Size: 1030 131 257 677 1148 245 2120 Fragments arranged by size: 2120 1148 1030 677 257 245 131 NarI GG'CG_CC Cuts at: 2203 2673 2203 Size: 470 5138 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5056 NdeI CA'TA_TG Cuts at: 387 387 Size: 5608 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5608 NotI GC'GGCC_GC Cuts at: 3055 3055 Size: 5608 NspI r_CATG'y Cuts at: 1132 1229 2966 4511 1132 Size: 97 1737 1545 2229 Fragments arranged by size: 2229 1737 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5608 PciI A'CATG_T Cuts at: 4507 4507 Size: 5608 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5608 PfoI T'CCnGG_A Cuts at: 2337 2808 2337 Size: 471 5137 PmeI GTTT'AAAC Cuts at: 2912 2912 Size: 5608 PmlI CAC'GTG Cuts at: 2874 2874 Size: 5608 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3677 3709 5190 5222 3677 Size: 32 1481 32 4063 Fragments arranged by size: 4063 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2292 2292 Size: 5608 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5608 PsiI TTA'TAA Cuts at: 3178 3178 Size: 5608 PspOMI G'GGCC_C Cuts at: 2468 2468 Size: 5608 PstI C_TGCA'G Cuts at: 830 2046 2137 2418 2960 830 Size: 1216 91 281 542 3478 Fragments arranged by size: 3478 1216 542 281 91 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5608 PvuII CAG'CTG Cuts at: 2636 2767 2636 Size: 131 5477 SacI G_AGCT'C Cuts at: 2663 2663 Size: 5608 SalI G'TCGA_C Cuts at: 1936 2950 1936 Size: 1014 4594 SbfI CC_TGCA'GG Cuts at: 2960 2960 Size: 5608 ScaI AGT'ACT Cuts at: 2048 3856 5568 2048 Size: 1808 1712 2088 Fragments arranged by size: 2088 1808 1712 SfcI C'TryA_G Cuts at: 826 1045 2042 2053 2133 2414 2956 4091 4772 Size: 219 997 11 80 281 542 1135 681 Cuts at: 4772 4963 826 Size: 191 1471 Fragments arranged by size: 1471 1135 997 681 542 281 219 191 80 11 SfoI GGC'GCC Cuts at: 2204 2674 2204 Size: 470 5138 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5608 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5608 SmaI CCC'GGG Cuts at: 1872 2935 1872 Size: 1063 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3712 4613 4875 820 Size: 197 926 1769 901 262 1553 Fragments arranged by size: 1769 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3399 493 Size: 2906 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5608 SphI G_CATG'C Cuts at: 1132 2966 1132 Size: 1834 3774 SspI AAT'ATT Cuts at: 5 52 3532 5 Size: 47 3480 2081 Fragments arranged by size: 3480 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5608 StyI C'CwwG_G Cuts at: 513 1065 3047 513 Size: 552 1982 3074 Fragments arranged by size: 3074 1982 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2112 2603 3635 3652 3805 3990 4435 Size: 1255 532 491 1032 17 153 185 445 Cuts at: 4435 325 Size: 1498 Fragments arranged by size: 1498 1255 1032 532 491 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2046 3854 5566 Size: 274 80 33 51 1443 69 1808 1712 Cuts at: 5566 96 Size: 138 Fragments arranged by size: 1808 1712 1443 274 138 80 69 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2228 2260 5568 5600 2228 Size: 32 3308 32 2236 Fragments arranged by size: 3308 2236 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5608 XbaI T'CTAG_A Cuts at: 2944 2944 Size: 5608 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5608 XmaI C'CCGG_G Cuts at: 1870 2933 1870 Size: 1063 4545 XmnI GAAnn'nnTTC Cuts at: 2409 2802 3737 2409 Size: 393 935 4280 Fragments arranged by size: 4280 935 393 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3416 276 Size: 53 83 186 936 1882 2468 Fragments arranged by size: 2468 1882 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI