(Circular) (Six-base) MAPSORT of: pFN21AB8341.seq Check: 8084 from: 1 to: 6532 pFN21AB8341 6532 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4342 278 Size: 53 83 186 936 2806 2468 Fragments arranged by size: 2806 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3875 1937 Size: 1938 4594 Acc65I G'GTAC_C Cuts at: 3853 3853 Size: 6532 AclI AA'CG_TT Cuts at: 1563 4659 5032 1563 Size: 3096 373 3063 Fragments arranged by size: 3096 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2526 4595 5979 6247 1632 Size: 894 2069 1384 268 1917 Fragments arranged by size: 2069 1917 1384 894 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6335 AflIII A'CryG_T Cuts at: 1203 2325 3764 5431 6408 1203 Size: 1122 1439 1667 977 1327 Fragments arranged by size: 1667 1439 1327 1122 977 AgeI A'CCGG_T Cuts at: 5363 5363 Size: 6532 AhdI GACnn_n'nnGTC Cuts at: 5261 5261 Size: 6532 AleI CACnn'nnGTG Cuts at: 1166 2209 2574 3579 1166 Size: 1043 365 1005 4119 Fragments arranged by size: 4119 1043 1005 365 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6532 AlwNI CAG_nnn'CTG Cuts at: 4275 5847 4275 Size: 1572 4960 ApaLI G'TGCA_C Cuts at: 4589 5745 4589 Size: 1156 5376 ApoI r'AATT_y Cuts at: 1493 2565 2649 2817 2901 3069 3237 3321 3405 Size: 1072 84 168 84 168 168 84 84 Cuts at: 3405 3486 3570 3841 4067 4219 6328 1493 Size: 81 84 271 226 152 2109 1697 Fragments arranged by size: 2109 1697 1072 271 226 168 168 168 152 84 84 84 84 84 81 AseI AT'TA_AT Cuts at: 160 1032 5086 160 Size: 872 4054 1606 Fragments arranged by size: 4054 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6532 AvaI C'yCGr_G Cuts at: 1870 1943 3857 1870 Size: 73 1914 4545 Fragments arranged by size: 4545 1914 73 BamHI G'GATC_C Cuts at: 3738 3862 4229 3738 Size: 124 367 6041 Fragments arranged by size: 6041 367 124 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3853 5308 618 Size: 325 217 627 2066 1455 1842 Fragments arranged by size: 2066 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3851 1391 Size: 2460 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3119 3452 928 Size: 971 1220 333 4008 Fragments arranged by size: 4008 1220 971 333 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4723 4757 1903 Size: 34 50 34 2702 34 3678 Fragments arranged by size: 3678 2702 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4424 5634 4424 Size: 1210 5322 BclI T'GATC_A Cuts at: 1548 1548 Size: 6532 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5143 136 Size: 107 122 71 631 605 3471 1525 Fragments arranged by size: 3471 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6527 6527 Size: 6532 BlpI GC'TnA_GC Cuts at: 3949 3949 Size: 6532 Bme1580I G_kGCm'C Cuts at: 1790 4593 5749 1790 Size: 2803 1156 2573 Fragments arranged by size: 2803 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6532 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4263 5221 442 Size: 416 53 83 399 24 2846 958 1753 Fragments arranged by size: 2846 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6532 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2160 2192 3684 5192 1239 Size: 921 32 1492 1508 2579 Fragments arranged by size: 2579 1508 1492 921 32 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4657 5522 5820 4657 Size: 865 298 5369 Fragments arranged by size: 5369 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3163 5195 882 Size: 617 1664 2032 2219 Fragments arranged by size: 2219 2032 1664 617 BsaAI yAC'GTr Cuts at: 493 1204 3458 4323 493 Size: 711 2254 865 2702 Fragments arranged by size: 2702 2254 865 711 BsaBI GATnn'nnATC Cuts at: 6526 6526 Size: 6532 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4339 4721 5356 275 Size: 53 83 186 936 2806 382 635 1451 Fragments arranged by size: 2806 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3245 4965 5363 5637 5784 6195 3245 Size: 1720 398 274 147 411 3582 Fragments arranged by size: 3582 1720 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6307 Fragments arranged by size: 6307 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6532 BseYI C'CCAG_C Cuts at: 5735 6231 5735 Size: 496 6036 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5959 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3982 4743 5771 1540 Size: 275 186 1981 761 1028 2301 Fragments arranged by size: 2301 1981 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4593 4678 5749 4593 Size: 85 1071 5376 Fragments arranged by size: 5376 1071 85 BsmI GAATG_Cn' Cuts at: 2072 2438 3110 4043 4136 2072 Size: 366 672 933 93 4468 Fragments arranged by size: 4468 933 672 366 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3851 4593 4678 5749 1391 Size: 399 2061 742 85 1071 2174 Fragments arranged by size: 2174 2061 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6195 6195 Size: 6532 BspHI T'CATG_A Cuts at: 4421 6151 4421 Size: 1730 4802 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3887 844 Size: 3043 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4419 1394 Size: 429 2596 3507 Fragments arranged by size: 3507 2596 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5027 5201 65 Size: 1183 3779 174 1396 Fragments arranged by size: 3779 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5176 5363 1700 Size: 110 3366 187 2869 Fragments arranged by size: 3366 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2094 2418 96 Size: 1998 324 4210 Fragments arranged by size: 4210 1998 324 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6532 BssSI C'ACGA_G Cuts at: 4592 5604 4592 Size: 1012 5520 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6532 BstBI TT'CG_AA Cuts at: 5336 5336 Size: 6532 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6532 BstYI r'GATC_y Cuts at: 1972 3738 3862 4229 4614 4631 6083 6169 6181 Size: 1766 124 367 385 17 1452 86 12 Cuts at: 6181 6527 1972 Size: 346 1977 Fragments arranged by size: 1977 1766 1452 385 367 346 124 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5980 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5793 Fragments arranged by size: 5793 683 56 BtsI GCAGTG_nn' Cuts at: 4048 4842 4862 4048 Size: 794 20 5718 Fragments arranged by size: 5718 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6532 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3172 3207 529 Size: 35 2608 35 3854 Fragments arranged by size: 3854 2608 35 35 DraI TTT'AAA Cuts at: 3836 3836 Size: 6532 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5539 809 Size: 507 4223 1802 Fragments arranged by size: 4223 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 3979 4868 Size: 54 1425 50 275 18 207 1942 889 Cuts at: 4868 6497 8 Size: 1629 43 Fragments arranged by size: 1942 1629 1425 889 275 207 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3979 1537 Size: 275 2167 4090 Fragments arranged by size: 4090 2167 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2383 2811 4462 1353 Size: 1030 428 1651 3423 Fragments arranged by size: 3423 1651 1030 428 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5119 5493 5639 387 Size: 1064 138 3530 374 146 1280 Fragments arranged by size: 3530 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2160 2192 2526 3684 4595 5192 5979 Size: 393 528 32 334 1158 911 597 787 Cuts at: 5979 6247 1239 Size: 268 1524 Fragments arranged by size: 1524 1158 911 787 597 528 393 334 268 32 EcoO109I rG'GnC_Cy Cuts at: 3699 3699 Size: 6532 EcoRI G'AATT_C Cuts at: 2565 2817 2901 3069 3237 3321 3486 3570 3841 Size: 252 84 168 168 84 165 84 271 Cuts at: 3841 4219 2565 Size: 378 4878 Fragments arranged by size: 4878 378 271 252 168 168 165 84 84 84 FspI TGC'GCA Cuts at: 5038 5038 Size: 6532 HaeII r_GCGC'y Cuts at: 1678 5679 1678 Size: 4001 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2132 2164 5173 5205 5247 Size: 32 135 32 560 32 3009 32 42 Cuts at: 5247 5279 1373 Size: 32 2626 Fragments arranged by size: 3009 2626 560 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3876 4122 669 Size: 988 281 1938 246 3079 Fragments arranged by size: 3079 1938 988 281 246 HindIII A'AGCT_T Cuts at: 748 2694 748 Size: 1946 4586 HpaI GTT'AAC Cuts at: 4122 4122 Size: 6532 KpnI G_GTAC'C Cuts at: 3857 3857 Size: 6532 MfeI C'AATT_G Cuts at: 4131 4131 Size: 6532 MluI A'CGCG_T Cuts at: 6408 6408 Size: 6532 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2091 5646 5830 1305 Size: 786 3555 184 2007 Fragments arranged by size: 3555 2007 786 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2039 10 Size: 54 1425 343 207 4503 Fragments arranged by size: 4503 1425 343 207 54 MslI CAynn'nnrTG Cuts at: 518 1166 2209 2574 2658 2758 2826 2910 2994 Size: 648 1043 365 84 100 68 84 84 Cuts at: 2994 3162 3330 3495 3579 3747 4490 4849 5008 Size: 168 168 165 84 168 743 359 159 Cuts at: 5008 518 Size: 2042 Fragments arranged by size: 2042 1043 743 648 365 359 168 168 168 165 159 100 84 84 84 84 68 MspA1I CmG'CkG Cuts at: 1606 3948 4625 5773 6018 1606 Size: 2342 677 1148 245 2120 Fragments arranged by size: 2342 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5980 NdeI CA'TA_TG Cuts at: 387 2755 3011 387 Size: 2368 256 3908 Fragments arranged by size: 3908 2368 256 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6532 NotI GC'GGCC_GC Cuts at: 3979 3979 Size: 6532 NspI r_CATG'y Cuts at: 1132 1229 2329 2688 3768 3890 5435 1132 Size: 97 1100 359 1080 122 1545 2229 Fragments arranged by size: 2229 1545 1100 1080 359 122 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6532 PciI A'CATG_T Cuts at: 2325 3764 5431 2325 Size: 1439 1667 3426 Fragments arranged by size: 3426 1667 1439 PflMI CCAn_nnn'nTGG Cuts at: 1108 2017 1108 Size: 909 5623 PmeI GTTT'AAAC Cuts at: 3836 3836 Size: 6532 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4601 4633 6114 6146 4601 Size: 32 1481 32 4987 Fragments arranged by size: 4987 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6532 PsiI TTA'TAA Cuts at: 3177 4102 3177 Size: 925 5607 PstI C_TGCA'G Cuts at: 830 3884 830 Size: 3054 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6532 SalI G'TCGA_C Cuts at: 1936 3874 1936 Size: 1938 4594 SbfI CC_TGCA'GG Cuts at: 3884 3884 Size: 6532 ScaI AGT'ACT Cuts at: 4780 6492 4780 Size: 1712 4820 SfcI C'TryA_G Cuts at: 826 1045 2119 3880 5015 5696 5887 826 Size: 219 1074 1761 1135 681 191 1471 Fragments arranged by size: 1761 1471 1135 1074 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6532 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6532 SmaI CCC'GGG Cuts at: 1872 3859 1872 Size: 1987 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4636 5537 5799 820 Size: 197 926 2693 901 262 1553 Fragments arranged by size: 2693 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4323 493 Size: 3830 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6532 SphI G_CATG'C Cuts at: 1132 3890 1132 Size: 2758 3774 SspI AAT'ATT Cuts at: 5 52 4456 5 Size: 47 4404 2081 Fragments arranged by size: 4404 2081 47 StuI AGG'CCT Cuts at: 1517 2612 2864 2948 3284 3368 3533 1517 Size: 1095 252 84 336 84 165 4516 Fragments arranged by size: 4516 1095 336 252 165 84 84 StyI C'CwwG_G Cuts at: 513 1065 2406 3971 513 Size: 552 1341 1565 3074 Fragments arranged by size: 3074 1565 1341 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4559 4576 4729 4914 5359 325 Size: 1255 2979 17 153 185 445 1498 Fragments arranged by size: 2979 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2094 2418 4778 Size: 274 80 33 51 1443 117 324 2360 Cuts at: 4778 6490 96 Size: 1712 138 Fragments arranged by size: 2360 1712 1443 324 274 138 117 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6492 6524 6492 Size: 32 6500 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6532 XbaI T'CTAG_A Cuts at: 3868 3868 Size: 6532 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6532 XmaI C'CCGG_G Cuts at: 1870 3857 1870 Size: 1987 4545 XmnI GAAnn'nnTTC Cuts at: 4661 4661 Size: 6532 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4340 276 Size: 53 83 186 936 2806 2468 Fragments arranged by size: 2806 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI