Restriction map for
pFN21AB8340
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# NO CUT ENZYMES ( 188 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
AluI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
BmyI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsiHKAI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraII DrdI DsaI Eam1105I
EarI Eco47III Eco81I EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FokI FseI FspI HgaI
Hin1I HindIII HpaI HphI KasI
KpnI Ksp632I KspI MaeI MaeII
MaeIII MamI MboII MfeI MluI
MluNI MroI MscI MslI MspA1I
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NlaIII NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SapI SbfI
ScaI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI Tsp45I Tsp509I TspRI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 70 GTCGAC GT^[AC][GT]AC
AfaI 54 GTAC GT^AC
AlwI 206 GATCC GGATC(4/5)
AlwNI 157 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 33 GGACC G^G[AT]CC
BbvI 156 GCAGC GCAGC(8/12)
BcnI 81 CCCGG CC^[GC]GG
BpmI 49 CTGGAG CTGGAG(16/14)
BsgI 201 GTGCAG GTGCAG(16/14)
BsiEI 110 CGGCCG CG[GA][CT]^CG
BsrBI 18 GAGCGG CCGCTC(-3/-3)
BstYI 205 AGATCC [GA]^GATC[CT]
DpnII 206 GATC ^GATC
DraIII 93 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 110 CGGCCG [CT]^GGCC[GA]
EagI 110 CGGCCG C^GGCCG
EclXI 110 CGGCCG C^GGCCG
Eco52I 110 CGGCCG C^GGCCG
EcoNI 123 CCTTTCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HapII 82 CCGG C^CGG
HincII 70 GTCGAC GT[CT]^[GA]AC
HindII 70 GTCGAC GT[CT]^[GA]AC
HinfI 85 GATTC G^A[ACGT]TC
HpaII 82 CCGG C^CGG
MboI 206 GATC ^GATC
MflI 205 AGATCC [GA]^GATC[CT]
MseI 152 TTAA T^TAA
MspI 82 CCGG C^CGG
NciI 81 CCCGG CC^[GC]GG
NdeII 206 GATC ^GATC
NlaIV 22 GGCCCC GG[ACGT]^[ACGT]CC
RsaI 54 GTAC GT^AC
SalI 70 GTCGAC G^TCGAC
Sau3AI 206 GATC ^GATC
SexAI 35 ACCAGGT A^CC[AT]GGT
TaqI 71 TCGA T^CGA
TfiI 85 GATTC G^A[AT]TC
Tru9I 152 TTAA T^TAA
TseI 156 GCAGC G^C[AT]GC
TthHB8I 71 TCGA T^CGA
XhoII 205 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AccII 15 CGCG CG^CG
108 CGCG
BsaI 10 GAGACC GGTCTC(1/5)
136 GAGACC
BsaJI 113 CCGAGG C^C[ACGT][ACGT]GG
128 CCGAGG
BseRI 130 GAGGAG GAGGAG(10/8)
133 GAGGAG
BsmAI 10 GAGAC GTCTC(1/5)
136 GAGAC
BstUI 15 CGCG CG^CG
108 CGCG
CfoI 159 GCGC GCG^C
177 GCGC
Cfr13I 22 GGCCC G^G[ACGT]CC
33 GGACC
DdeI 180 CTCAG C^T[ACGT]AG
195 CTCAG
HaeII 158 AGCGCC [GA]GCGC^[CT]
176 GGCGCT
HhaI 159 GCGC GCG^C
177 GCGC
HinP1I 159 GCGC G^CGC
177 GCGC
MvnI 15 CGCG CG^CG
108 CGCG
Sau96I 22 GGCCC G^G[ACGT]CC
33 GGACC
StuI 120 AGGCCT AGG^CCT
186 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AciI BsiYI BslI BssKI BstNI
Cac8I EcoRII Fnu4HI HaeIII ItaI
MnlI MvaI MwoI ScrFI