(Circular) (Six-base) MAPSORT of: pFN21AB8339.seq Check: 5475 from: 1 to: 5635 pFN21AB8339 5635 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3445 278 Size: 53 83 186 936 1909 2468 Fragments arranged by size: 2468 1909 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2978 1937 Size: 1041 4594 Acc65I G'GTAC_C Cuts at: 2393 2956 2393 Size: 563 5072 AclI AA'CG_TT Cuts at: 1563 2934 3762 4135 1563 Size: 1371 828 373 3063 Fragments arranged by size: 3063 1371 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2811 3698 5082 5350 1632 Size: 1179 887 1384 268 1917 Fragments arranged by size: 1917 1384 1179 887 268 AfeI AGC'GCT Cuts at: 2531 2531 Size: 5635 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5438 AflIII A'CryG_T Cuts at: 1203 4534 5511 1203 Size: 3331 977 1327 Fragments arranged by size: 3331 1327 977 AgeI A'CCGG_T Cuts at: 4466 4466 Size: 5635 AhdI GACnn_n'nnGTC Cuts at: 4364 4364 Size: 5635 AleI CACnn'nnGTG Cuts at: 1166 2372 1166 Size: 1206 4429 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5635 AlwNI CAG_nnn'CTG Cuts at: 2339 2417 3378 4950 2339 Size: 78 961 1572 3024 Fragments arranged by size: 3024 1572 961 78 ApaLI G'TGCA_C Cuts at: 3692 4848 3692 Size: 1156 4479 ApoI r'AATT_y Cuts at: 1493 2944 3170 3322 5431 1493 Size: 1451 226 152 2109 1697 Fragments arranged by size: 2109 1697 1451 226 152 AseI AT'TA_AT Cuts at: 160 1032 4189 160 Size: 872 3157 1606 Fragments arranged by size: 3157 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5635 AvaI C'yCGr_G Cuts at: 1870 1943 2589 2960 1870 Size: 73 646 371 4545 Fragments arranged by size: 4545 646 371 73 AvrII C'CTAG_G Cuts at: 2543 2543 Size: 5635 BamHI G'GATC_C Cuts at: 2965 3332 2965 Size: 367 5268 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2240 2393 2895 2956 4411 Size: 325 217 627 453 153 502 61 1455 Cuts at: 4411 618 Size: 1842 Fragments arranged by size: 1842 1455 627 502 453 325 217 153 61 BanII G_rGCy'C Cuts at: 1391 2438 2611 2887 2954 1391 Size: 1047 173 276 67 4072 Fragments arranged by size: 4072 1047 276 173 67 BbeI G_GCGC'C Cuts at: 2244 2244 Size: 5635 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2088 2146 928 Size: 971 189 58 4417 Fragments arranged by size: 4417 971 189 58 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3826 3860 1903 Size: 34 1889 34 3678 Fragments arranged by size: 3678 1889 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3527 4737 3527 Size: 1210 4425 BclI T'GATC_A Cuts at: 1548 1548 Size: 5635 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4246 136 Size: 107 122 71 631 605 2574 1525 Fragments arranged by size: 2574 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2854 5630 2854 Size: 2776 2859 BlpI GC'TnA_GC Cuts at: 2610 3052 2610 Size: 442 5193 Bme1580I G_kGCm'C Cuts at: 1790 3696 4852 1790 Size: 1906 1156 2573 Fragments arranged by size: 2573 1906 1156 BmgBI CAC'GTC Cuts at: 1318 2833 1318 Size: 1515 4120 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2085 3366 4324 Size: 416 53 83 399 24 668 1281 958 Cuts at: 4324 442 Size: 1753 Fragments arranged by size: 1753 1281 958 668 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5635 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4295 1239 Size: 3056 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3760 4625 4923 3760 Size: 865 298 4472 Fragments arranged by size: 4472 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4298 882 Size: 617 2799 2219 Fragments arranged by size: 2799 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3426 493 Size: 711 2222 2702 Fragments arranged by size: 2702 2222 711 BsaBI GATnn'nnATC Cuts at: 5629 5629 Size: 5635 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2241 3442 3824 4459 Size: 53 83 186 936 708 1201 382 635 Cuts at: 4459 275 Size: 1451 Fragments arranged by size: 1451 1201 936 708 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4068 4466 4740 4887 5298 4068 Size: 398 274 147 411 4405 Fragments arranged by size: 4405 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5410 Fragments arranged by size: 5410 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5635 BseYI C'CCAG_C Cuts at: 2363 2449 4838 5334 2363 Size: 86 2389 496 2664 Fragments arranged by size: 2664 2389 496 86 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2069 2085 2684 1163 Size: 573 333 16 599 4114 Fragments arranged by size: 4114 599 573 333 16 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3085 3846 4874 1540 Size: 275 186 1084 761 1028 2301 Fragments arranged by size: 2301 1084 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2705 2887 3696 3781 4852 2705 Size: 182 809 85 1071 3488 Fragments arranged by size: 3488 1071 809 182 85 BsmI GAATG_Cn' Cuts at: 3146 3239 3146 Size: 93 5542 BsmBI CGTCTCn'nnnn_ Cuts at: 2336 2336 Size: 5635 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2438 2611 2705 2887 2954 3696 3781 Size: 399 648 173 94 182 67 742 85 Cuts at: 3781 4852 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 648 399 182 173 94 85 67 BspEI T'CCGG_A Cuts at: 5298 5298 Size: 5635 BspHI T'CATG_A Cuts at: 3524 5254 3524 Size: 1730 3905 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2990 844 Size: 2146 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3522 1394 Size: 429 1699 3507 Fragments arranged by size: 3507 1699 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4130 4304 65 Size: 1183 2882 174 1396 Fragments arranged by size: 2882 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4279 4466 1700 Size: 110 2469 187 2869 Fragments arranged by size: 2869 2469 187 110 BsrGI T'GTAC_A Cuts at: 96 2184 96 Size: 2088 3547 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5635 BssSI C'ACGA_G Cuts at: 3695 4707 3695 Size: 1012 4623 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5635 BstBI TT'CG_AA Cuts at: 4439 4439 Size: 5635 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5635 BstYI r'GATC_y Cuts at: 1972 2854 2965 3332 3717 3734 5186 5272 5284 Size: 882 111 367 385 17 1452 86 12 Cuts at: 5284 5630 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 882 385 367 346 111 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2899 513 Size: 552 1834 3249 Fragments arranged by size: 3249 1834 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4896 Fragments arranged by size: 4896 683 56 BtsI GCAGTG_nn' Cuts at: 2073 2379 2444 3151 3945 3965 2073 Size: 306 65 707 794 20 3743 Fragments arranged by size: 3743 794 707 306 65 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5635 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2076 2111 529 Size: 35 1512 35 4053 Fragments arranged by size: 4053 1512 35 35 DraI TTT'AAA Cuts at: 2939 2939 Size: 5635 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2141 4642 809 Size: 507 825 2501 1802 Fragments arranged by size: 2501 1802 825 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2221 2713 3082 Size: 54 1425 50 275 18 391 492 369 Cuts at: 3082 3971 5600 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 492 391 369 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3082 1537 Size: 275 1270 4090 Fragments arranged by size: 4090 1270 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2031 3565 1353 Size: 678 1534 3423 Fragments arranged by size: 3423 1534 678 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4222 4596 4742 387 Size: 1064 138 2633 374 146 1280 Fragments arranged by size: 2633 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2885 2885 Size: 5635 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2811 3698 4295 5082 5350 1239 Size: 393 1179 887 597 787 268 1524 Fragments arranged by size: 1524 1179 887 787 597 393 268 EcoRI G'AATT_C Cuts at: 2944 3322 2944 Size: 378 5257 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2170 2170 Size: 5635 FspI TGC'GCA Cuts at: 4141 4141 Size: 5635 HaeII r_GCGC'y Cuts at: 1678 2244 2533 4782 1678 Size: 566 289 2249 2531 Fragments arranged by size: 2531 2249 566 289 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2631 2663 2895 2927 4276 Size: 32 135 32 1059 32 232 32 1349 Cuts at: 4276 4308 4350 4382 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1349 1059 232 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2320 2979 3225 669 Size: 988 281 382 659 246 3079 Fragments arranged by size: 3079 988 659 382 281 246 HindIII A'AGCT_T Cuts at: 748 2742 748 Size: 1994 3641 HpaI GTT'AAC Cuts at: 3225 3225 Size: 5635 KasI G'GCGC_C Cuts at: 2240 2240 Size: 5635 KpnI G_GTAC'C Cuts at: 2397 2960 2397 Size: 563 5072 MfeI C'AATT_G Cuts at: 2535 3234 2535 Size: 699 4936 MluI A'CGCG_T Cuts at: 5511 5511 Size: 5635 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4749 4933 1305 Size: 3444 184 2007 Fragments arranged by size: 3444 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2223 2715 10 Size: 54 1425 343 391 492 2930 Fragments arranged by size: 2930 1425 492 391 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2372 3593 3952 4111 518 Size: 648 1206 1221 359 159 2042 Fragments arranged by size: 2042 1221 1206 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2027 2120 2215 2336 2414 2821 3051 3728 Size: 421 93 95 121 78 407 230 677 Cuts at: 3728 4876 5121 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 421 407 245 230 121 95 93 78 NarI GG'CG_CC Cuts at: 2241 2241 Size: 5635 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5083 NdeI CA'TA_TG Cuts at: 387 387 Size: 5635 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5635 NotI GC'GGCC_GC Cuts at: 3082 3082 Size: 5635 NspI r_CATG'y Cuts at: 1132 1229 2993 4538 1132 Size: 97 1764 1545 2229 Fragments arranged by size: 2229 1764 1545 97 PasI CC'CwG_GG Cuts at: 1348 2431 1348 Size: 1083 4552 PciI A'CATG_T Cuts at: 4534 4534 Size: 5635 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5635 PfoI T'CCnGG_A Cuts at: 2757 2757 Size: 5635 PmeI GTTT'AAAC Cuts at: 2939 2939 Size: 5635 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3704 3736 5217 5249 3704 Size: 32 1481 32 4090 Fragments arranged by size: 4090 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5635 PsiI TTA'TAA Cuts at: 3205 3205 Size: 5635 PstI C_TGCA'G Cuts at: 830 2517 2802 2820 2987 830 Size: 1687 285 18 167 3478 Fragments arranged by size: 3478 1687 285 167 18 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5635 PvuII CAG'CTG Cuts at: 2027 2120 2215 2336 2414 2821 2027 Size: 93 95 121 78 407 4841 Fragments arranged by size: 4841 407 121 95 93 78 SacI G_AGCT'C Cuts at: 2887 2887 Size: 5635 SalI G'TCGA_C Cuts at: 1936 2977 1936 Size: 1041 4594 SbfI CC_TGCA'GG Cuts at: 2987 2987 Size: 5635 ScaI AGT'ACT Cuts at: 2511 3883 5595 2511 Size: 1372 1712 2551 Fragments arranged by size: 2551 1712 1372 SfcI C'TryA_G Cuts at: 826 1045 2513 2798 2816 2983 4118 4799 4990 Size: 219 1468 285 18 167 1135 681 191 Cuts at: 4990 826 Size: 1471 Fragments arranged by size: 1471 1468 1135 681 285 219 191 167 18 SfoI GGC'GCC Cuts at: 2242 2242 Size: 5635 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5635 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5635 SmaI CCC'GGG Cuts at: 1872 2962 1872 Size: 1090 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3739 4640 4902 820 Size: 197 926 1796 901 262 1553 Fragments arranged by size: 1796 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3426 493 Size: 2933 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5635 SphI G_CATG'C Cuts at: 1132 2993 1132 Size: 1861 3774 SspI AAT'ATT Cuts at: 5 52 3559 5 Size: 47 3507 2081 Fragments arranged by size: 3507 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5635 StyI C'CwwG_G Cuts at: 513 1065 2543 3074 513 Size: 552 1478 531 3074 Fragments arranged by size: 3074 1478 552 531 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3662 3679 3832 4017 4462 325 Size: 1255 2082 17 153 185 445 1498 Fragments arranged by size: 2082 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2184 2408 2509 Size: 274 80 33 51 1443 207 224 101 Cuts at: 2509 3881 5593 96 Size: 1372 1712 138 Fragments arranged by size: 1712 1443 1372 274 224 207 138 101 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2543 2575 5595 5627 2543 Size: 32 3020 32 2551 Fragments arranged by size: 3020 2551 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5635 XbaI T'CTAG_A Cuts at: 2971 2971 Size: 5635 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2371 2723 2371 Size: 352 5283 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5635 XmaI C'CCGG_G Cuts at: 1870 2960 1870 Size: 1090 4545 XmnI GAAnn'nnTTC Cuts at: 3764 3764 Size: 5635 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3443 276 Size: 53 83 186 936 1909 2468 Fragments arranged by size: 2468 1909 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI