(Circular) (Six-base) MAPSORT of: pFN21AB8338.seq Check: 2548 from: 1 to: 5521 pFN21AB8338 5521 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3331 278 Size: 53 83 186 936 1795 2468 Fragments arranged by size: 2468 1795 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2864 1937 Size: 927 4594 Acc65I G'GTAC_C Cuts at: 2842 2842 Size: 5521 AclI AA'CG_TT Cuts at: 1563 3648 4021 1563 Size: 2085 373 3063 Fragments arranged by size: 3063 2085 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2140 3584 4968 5236 1632 Size: 508 1444 1384 268 1917 Fragments arranged by size: 1917 1444 1384 508 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5324 AflIII A'CryG_T Cuts at: 1203 2688 4420 5397 1203 Size: 1485 1732 977 1327 Fragments arranged by size: 1732 1485 1327 977 AgeI A'CCGG_T Cuts at: 4352 4352 Size: 5521 AhdI GACnn_n'nnGTC Cuts at: 4250 4250 Size: 5521 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5521 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2342 1184 Size: 1158 4363 AlwNI CAG_nnn'CTG Cuts at: 2659 3264 4836 2659 Size: 605 1572 3344 Fragments arranged by size: 3344 1572 605 ApaI G_GGCC'C Cuts at: 2023 2023 Size: 5521 ApaLI G'TGCA_C Cuts at: 2213 2279 3578 4734 2213 Size: 66 1299 1156 3000 Fragments arranged by size: 3000 1299 1156 66 ApoI r'AATT_y Cuts at: 1493 2830 3056 3208 5317 1493 Size: 1337 226 152 2109 1697 Fragments arranged by size: 2109 1697 1337 226 152 AseI AT'TA_AT Cuts at: 160 1032 4075 160 Size: 872 3043 1606 Fragments arranged by size: 3043 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5521 AvaI C'yCGr_G Cuts at: 1870 1943 2846 1870 Size: 73 903 4545 Fragments arranged by size: 4545 903 73 BamHI G'GATC_C Cuts at: 2851 3218 2851 Size: 367 5154 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2057 2195 2225 2290 2308 Size: 325 217 627 270 138 30 65 18 Cuts at: 2308 2522 2634 2842 4297 618 Size: 214 112 208 1455 1842 Fragments arranged by size: 1842 1455 627 325 270 217 214 208 138 112 65 30 18 BanII G_rGCy'C Cuts at: 1391 2023 2346 2840 1391 Size: 632 323 494 4072 Fragments arranged by size: 4072 632 494 323 BbeI G_GCGC'C Cuts at: 2061 2199 2229 2294 2312 2526 2061 Size: 138 30 65 18 214 5056 Fragments arranged by size: 5056 214 138 65 30 18 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4550 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3712 3746 1903 Size: 34 1775 34 3678 Fragments arranged by size: 3678 1775 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3413 4623 3413 Size: 1210 4311 BclI T'GATC_A Cuts at: 1548 1548 Size: 5521 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2122 4132 136 Size: 107 122 71 631 605 450 2010 1525 Fragments arranged by size: 2010 1525 631 605 450 122 107 71 BglII A'GATC_T Cuts at: 2757 5516 2757 Size: 2759 2762 BlpI GC'TnA_GC Cuts at: 2078 2938 2078 Size: 860 4661 Bme1580I G_kGCm'C Cuts at: 1790 2023 2217 2283 2639 3582 4738 1790 Size: 233 194 66 356 943 1156 2573 Fragments arranged by size: 2573 1156 943 356 233 194 66 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5521 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2025 2204 3252 Size: 416 53 83 399 24 608 179 1048 Cuts at: 3252 4210 442 Size: 958 1753 Fragments arranged by size: 1753 1048 958 608 416 399 179 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5521 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2360 2365 2475 2687 4181 1239 Size: 1121 5 110 212 1494 2579 Fragments arranged by size: 2579 1494 1121 212 110 5 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3646 4511 4809 3646 Size: 865 298 4358 Fragments arranged by size: 4358 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4184 882 Size: 617 2685 2219 Fragments arranged by size: 2685 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2038 2278 3312 493 Size: 711 834 240 1034 2702 Fragments arranged by size: 2702 1034 834 711 240 BsaBI GATnn'nnATC Cuts at: 5515 5515 Size: 5521 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2058 2064 2196 2226 Size: 53 83 186 936 525 6 132 30 Cuts at: 2226 2291 2309 2523 3328 3710 4345 275 Size: 65 18 214 805 382 635 1451 Fragments arranged by size: 1451 936 805 635 525 382 214 186 132 83 65 53 30 18 6 BsaWI w'CCGG_w Cuts at: 3954 4352 4626 4773 5184 3954 Size: 398 274 147 411 4291 Fragments arranged by size: 4291 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2315 2345 2660 2690 1178 Size: 30 165 30 912 30 315 30 4009 Fragments arranged by size: 4009 912 315 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2478 1186 Size: 1292 4229 BseYI C'CCAG_C Cuts at: 2053 2513 2590 4724 5220 2053 Size: 460 77 2134 496 2354 Fragments arranged by size: 2354 2134 496 460 77 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2185 1163 Size: 573 449 4499 Fragments arranged by size: 4499 573 449 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2971 3732 4760 1540 Size: 275 186 970 761 1028 2301 Fragments arranged by size: 2301 1028 970 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2148 2217 2283 3582 3667 4738 2148 Size: 69 66 1299 85 1071 2931 Fragments arranged by size: 2931 1299 1071 85 69 66 BsmI GAATG_Cn' Cuts at: 3032 3125 3032 Size: 93 5428 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2023 2148 2217 2283 2346 2639 2840 Size: 399 233 125 69 66 63 293 201 Cuts at: 2840 3582 3667 4738 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 293 233 201 125 85 69 66 63 BspEI T'CCGG_A Cuts at: 5184 5184 Size: 5521 BspHI T'CATG_A Cuts at: 3410 5140 3410 Size: 1730 3791 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2310 2700 2740 2876 844 Size: 1466 390 40 136 3489 Fragments arranged by size: 3489 1466 390 136 40 BsrBI CCG'CTC Cuts at: 1394 1823 2556 3408 1394 Size: 429 733 852 3507 Fragments arranged by size: 3507 852 733 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4016 4190 65 Size: 1183 2768 174 1396 Fragments arranged by size: 2768 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2385 4165 4352 1700 Size: 110 575 1780 187 2869 Fragments arranged by size: 2869 1780 575 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5521 BssHII G'CGCG_C Cuts at: 1925 2612 1925 Size: 687 4834 BssSI C'ACGA_G Cuts at: 2200 3581 4593 2200 Size: 1381 1012 3128 Fragments arranged by size: 3128 1381 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5521 BstBI TT'CG_AA Cuts at: 4325 4325 Size: 5521 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5521 BstYI r'GATC_y Cuts at: 1972 2757 2851 3218 3603 3620 5072 5158 5170 Size: 785 94 367 385 17 1452 86 12 Cuts at: 5170 5516 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 785 385 367 346 94 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2067 2295 513 Size: 552 939 63 228 3739 Fragments arranged by size: 3739 939 552 228 63 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4782 Fragments arranged by size: 4782 683 56 BtsI GCAGTG_nn' Cuts at: 3037 3831 3851 3037 Size: 794 20 4707 Fragments arranged by size: 4707 794 20 ClaI AT'CG_AT Cuts at: 1554 2577 1554 Size: 1023 4498 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5486 DraI TTT'AAA Cuts at: 2825 2825 Size: 5521 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4528 809 Size: 507 3212 1802 Fragments arranged by size: 3212 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2086 2139 Size: 54 1425 50 275 18 177 79 53 Cuts at: 2139 2418 2968 3857 5486 8 Size: 279 550 889 1629 43 Fragments arranged by size: 1629 1425 889 550 279 275 177 79 54 53 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2968 1537 Size: 275 1156 4090 Fragments arranged by size: 4090 1156 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3451 1353 Size: 2098 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2574 4108 4482 4628 387 Size: 1064 138 985 1534 374 146 1280 Fragments arranged by size: 1534 1280 1064 985 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2140 2360 2365 2475 2687 3584 4181 Size: 393 508 220 5 110 212 897 597 Cuts at: 4181 4968 5236 1239 Size: 787 268 1524 Fragments arranged by size: 1524 897 787 597 508 393 268 220 212 110 5 EcoO109I rG'GnC_Cy Cuts at: 2019 2020 2103 2019 Size: 1 83 5437 Fragments arranged by size: 5437 83 1 EcoRI G'AATT_C Cuts at: 2830 3208 2830 Size: 378 5143 FspI TGC'GCA Cuts at: 4027 4027 Size: 5521 HaeII r_GCGC'y Cuts at: 1678 2061 2199 2229 2294 2312 2526 4668 1678 Size: 383 138 30 65 18 214 2142 2531 Fragments arranged by size: 2531 2142 383 214 138 65 30 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4162 4194 4236 4268 1373 Size: 32 135 32 2590 32 42 32 2626 Fragments arranged by size: 2626 2590 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2865 3111 669 Size: 988 281 927 246 3079 Fragments arranged by size: 3079 988 927 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5521 HpaI GTT'AAC Cuts at: 3111 3111 Size: 5521 KasI G'GCGC_C Cuts at: 2057 2195 2225 2290 2308 2522 2057 Size: 138 30 65 18 214 5056 Fragments arranged by size: 5056 214 138 65 30 18 KpnI G_GTAC'C Cuts at: 2846 2846 Size: 5521 MfeI C'AATT_G Cuts at: 3120 3120 Size: 5521 MluI A'CGCG_T Cuts at: 5397 5397 Size: 5521 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4635 4819 1305 Size: 3330 184 2007 Fragments arranged by size: 3330 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 3522 Fragments arranged by size: 3522 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 3479 3838 3997 518 Size: 648 2313 359 159 2042 Fragments arranged by size: 2313 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2069 2243 2413 2937 3614 4762 5007 1606 Size: 463 174 170 524 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 524 463 245 174 170 NaeI GCC'GGC Cuts at: 2387 2387 Size: 5521 NarI GG'CG_CC Cuts at: 2058 2196 2226 2291 2309 2523 2058 Size: 138 30 65 18 214 5056 Fragments arranged by size: 5056 214 138 65 30 18 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4030 Fragments arranged by size: 4030 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5521 NgoMIV G'CCGG_C Cuts at: 2385 2385 Size: 5521 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5521 NotI GC'GGCC_GC Cuts at: 2968 2968 Size: 5521 NspI r_CATG'y Cuts at: 1132 1229 2329 2692 2879 4424 1132 Size: 97 1100 363 187 1545 2229 Fragments arranged by size: 2229 1545 1100 363 187 97 PasI CC'CwG_GG Cuts at: 1348 2023 2107 1348 Size: 675 84 4762 Fragments arranged by size: 4762 675 84 PciI A'CATG_T Cuts at: 2688 4420 2688 Size: 1732 3789 PflMI CCAn_nnn'nTGG Cuts at: 1108 2659 1108 Size: 1551 3970 PmeI GTTT'AAAC Cuts at: 2825 2825 Size: 5521 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2666 2698 3590 3622 5103 5135 2666 Size: 32 892 32 1481 32 3052 Fragments arranged by size: 3052 1481 892 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2103 2103 Size: 5521 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5521 PsiI TTA'TAA Cuts at: 3091 3091 Size: 5521 PspOMI G'GGCC_C Cuts at: 2019 2019 Size: 5521 PstI C_TGCA'G Cuts at: 830 2873 830 Size: 2043 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5521 SacII CC_GC'GG Cuts at: 2070 2070 Size: 5521 SalI G'TCGA_C Cuts at: 1936 2863 1936 Size: 927 4594 SanDI GG'GwC_CC Cuts at: 2103 2103 Size: 5521 SbfI CC_TGCA'GG Cuts at: 2873 2873 Size: 5521 ScaI AGT'ACT Cuts at: 3769 5481 3769 Size: 1712 3809 SfcI C'TryA_G Cuts at: 826 1045 2869 4004 4685 4876 826 Size: 219 1824 1135 681 191 1471 Fragments arranged by size: 1824 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2059 2197 2227 2292 2310 2524 2059 Size: 138 30 65 18 214 5056 Fragments arranged by size: 5056 214 138 65 30 18 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5521 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5521 SmaI CCC'GGG Cuts at: 1872 2848 1872 Size: 976 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3625 4526 4788 820 Size: 197 926 1682 901 262 1553 Fragments arranged by size: 1682 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3312 493 Size: 2819 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5521 SphI G_CATG'C Cuts at: 1132 2879 1132 Size: 1747 3774 SspI AAT'ATT Cuts at: 5 52 3445 5 Size: 47 3393 2081 Fragments arranged by size: 3393 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5521 StyI C'CwwG_G Cuts at: 513 1065 2004 2178 2960 513 Size: 552 939 174 782 3074 Fragments arranged by size: 3074 939 782 552 174 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2820 3548 3565 3718 3903 4348 325 Size: 1255 1240 728 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1240 728 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3767 5479 96 Size: 274 80 33 51 1443 1790 1712 138 Fragments arranged by size: 1790 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2444 2476 5481 5513 2444 Size: 32 3005 32 2452 Fragments arranged by size: 3005 2452 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5521 XbaI T'CTAG_A Cuts at: 2857 2857 Size: 5521 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2020 2598 2020 Size: 578 4943 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5521 XmaI C'CCGG_G Cuts at: 1870 2846 1870 Size: 976 4545 XmnI GAAnn'nnTTC Cuts at: 3650 3650 Size: 5521 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3329 276 Size: 53 83 186 936 1795 2468 Fragments arranged by size: 2468 1795 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SapI SexAI SfiI SrfI SwaI