Restriction map for
pFN21AB8337
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AviII AvrII BalI
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BseAI BseRI BsiEI
BsiWI BsmBI BspDI BspEI BsrBI
BssHII Bst1107I BstBI BstUI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoRV EcoT14I EcoT22I
FseI FspI HgaI Hin1I HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI ScaI SexAI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
SspI StyI SwaI XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AflIII 1571 ACATGT A^C[GA][CT]GT
AgeI 857 ACCGGT A^CCGGT
AocI 480 CCTCAGG CC^T[ACGT]AGG
ApaI 93 GGGCCC GGGCC^C
AspI 1076 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 71 CCCGGG C^[CT]CG[GA]G
BamHI 1505 GGATCC G^GATCC
BanI 241 GGCACC G^G[CT][GA]CC
BbsI 876 GAAGAC GAAGAC(2/6)
BclI 507 TGATCA T^GATCA
BglI 86 GCCGCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 876 GAAGAC GAAGAC(2/6)
BsaAI 1482 CACGTA [CT]AC^GT[GA]
BsmI 1279 GAATGC GAATGC(1/-1)
BsoBI 71 CCCGGG C^[CT]CG[GA]G
Bsp1407I 582 TGTACA T^GTACA
BspHI 902 TCATGA T^CATGA
BspLU11I 1571 ACATGT A^CATGT
BspMI 168 ACCTGC ACCTGC(4/8)
BsrDI 1283 GCAATG GCAATG(2/0)
BsrFI 857 ACCGGT [GA]^CCGG[CT]
BsrGI 582 TGTACA T^GTACA
BssSI 1237 CTCGTG CACGAG(-5/-1)
BstAPI 1451 GCAGCGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 39 GGTGACC G^GT[ACGT]ACC
BstPI 39 GGTGACC G^GT[ACGT]ACC
BstXI 1232 CCAAACTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 480 CCTCAGG CC^T[ACGT]AGG
Cac8I 172 GCAGGC GC[ACGT]^[ACGT]GC
CfoI 971 GCGC GCG^C
Cfr10I 857 ACCGGT [GA]^CCGG[CT]
DsaI 1428 CCACGG C^C[GA][CT]GG
Eco81I 480 CCTCAGG CC^T[ACGT]AGG
EcoNI 1596 CCTCCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 39 GGTGACC G^GT[ACGT]ACC
EcoRI 1169 GAATTC G^AATTC
FauI 1040 GCGGG CCCGC(4/6)
FbaI 507 TGATCA T^GATCA
HaeII 970 AGCGCC [GA]GCGC^[CT]
HhaI 971 GCGC GCG^C
HinP1I 971 GCGC G^CGC
HindIII 1214 AAGCTT A^AGCTT
MseI 650 TTAA T^TAA
NspI 1571 ACATGT [GA]CATG^[CT]
PflFI 1076 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 277 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 857 ACCGGT A^CCGGT
PstI 170 CTGCAG CTGCA^G
RcaI 902 TCATGA T^CATGA
SbfI 169 CCTGCAGG CCTGCA^GG
SfiI 85 GGCCGCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 71 CCCGGG CCC^GGG
Sse8387I 169 CCTGCAGG CCTGCA^GG
SspBI 582 TGTACA T^GTACA
TaqI 47 TCGA T^CGA
Tru9I 650 TTAA T^TAA
Tth111I 1076 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 47 TCGA T^CGA
Van91I 277 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 71 CCCGGG C^CCGGG
XmaI 71 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 665 AAATTC [GA]^AATT[CT]
1169 GAATTC
AlwNI 696 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
1417 CAGAGGCTG
ApoI 665 AAATTC [GA]^AATT[CT]
1169 GAATTC
AspHI 256 GAGCAC G[AT]GC[AT]^C
1479 GAGCAC
BanII 93 GGGCCC G[GA]GC[CT]^C
217 GAGCCC
BfaI 148 CTAG C^TAG
409 CTAG
BsaI 133 GAGACC GGTCTC(1/5)
513 GAGACC
BsiHKAI 256 GAGCAC G[AT]GC[AT]^C
1479 GAGCAC
BsmFI 119 GGGAC GGGAC(10/14)
286 GGGAC
EarI 978 CTCTTC CTCTTC(1/4)
760 GAAGAG
HincII 268 GTCAAC GT[CT]^[GA]AC
309 GTTGAC
HindII 268 GTCAAC GT[CT]^[GA]AC
309 GTTGAC
Ksp632I 978 CTCTTC CTCTTC(1/4)
760 GAAGAG
MaeI 148 CTAG C^TAG
409 CTAG
MspA1I 745 CAGCGG C[AC]G^C[GT]G
1585 CAGCGG
PpuMI 165 AGGACCT [GA]G^G[AT]CC[CT]
224 GGGTCCT
StuI 1382 AGGCCT AGG^CCT
1550 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AfaI AluI AlwI Asp700I
AvaII BbvI BcnI BmyI BpmI
BsaJI BsaWI BsgI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TfiI TseI Tsp45I
Tsp509I TspRI XhoII XmnI