(Circular) (Six-base) MAPSORT of: pFN21AB8337.seq Check: 911 from: 1 to: 6322 pFN21AB8337 6322 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4132 278 Size: 53 83 186 936 2596 2468 Fragments arranged by size: 2596 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3665 1937 Size: 1728 4594 Acc65I G'GTAC_C Cuts at: 3643 3643 Size: 6322 AclI AA'CG_TT Cuts at: 1563 4449 4822 1563 Size: 2886 373 3063 Fragments arranged by size: 3063 2886 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2620 2784 2788 3040 3460 4385 5769 6037 Size: 988 164 4 252 420 925 1384 268 Cuts at: 6037 1632 Size: 1917 Fragments arranged by size: 1917 1384 988 925 420 268 252 164 4 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6125 AflIII A'CryG_T Cuts at: 1203 3576 5221 6198 1203 Size: 2373 1645 977 1327 Fragments arranged by size: 2373 1645 1327 977 AgeI A'CCGG_T Cuts at: 2862 5153 2862 Size: 2291 4031 AhdI GACnn_n'nnGTC Cuts at: 5051 5051 Size: 6322 AleI CACnn'nnGTG Cuts at: 1166 2529 1166 Size: 1363 4959 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6322 AlwNI CAG_nnn'CTG Cuts at: 2706 3427 4065 5637 2706 Size: 721 638 1572 3391 Fragments arranged by size: 3391 1572 721 638 ApaI G_GGCC'C Cuts at: 2102 2102 Size: 6322 ApaLI G'TGCA_C Cuts at: 4379 5535 4379 Size: 1156 5166 ApoI r'AATT_y Cuts at: 1493 2670 3174 3631 3857 4009 6118 1493 Size: 1177 504 457 226 152 2109 1697 Fragments arranged by size: 2109 1697 1177 504 457 226 152 AseI AT'TA_AT Cuts at: 160 1032 4876 160 Size: 872 3844 1606 Fragments arranged by size: 3844 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6322 AvaI C'yCGr_G Cuts at: 1870 1943 2076 3647 1870 Size: 73 133 1571 4545 Fragments arranged by size: 4545 1571 133 73 BamHI G'GATC_C Cuts at: 3510 3652 4019 3510 Size: 142 367 5813 Fragments arranged by size: 5813 367 142 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2246 3643 5098 618 Size: 325 217 627 459 1397 1455 1842 Fragments arranged by size: 1842 1455 1397 627 459 325 217 BanII G_rGCy'C Cuts at: 1391 2102 2226 3641 1391 Size: 711 124 1415 4072 Fragments arranged by size: 4072 1415 711 124 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2888 928 Size: 971 989 4362 Fragments arranged by size: 4362 989 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4513 4547 1903 Size: 34 2576 34 3678 Fragments arranged by size: 3678 2576 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4214 5424 4214 Size: 1210 5112 BclI T'GATC_A Cuts at: 1548 2512 1548 Size: 964 5358 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2097 4933 Size: 107 122 71 631 605 337 88 2836 Cuts at: 4933 136 Size: 1525 Fragments arranged by size: 2836 1525 631 605 337 122 107 88 71 BglII A'GATC_T Cuts at: 6317 6317 Size: 6322 BlpI GC'TnA_GC Cuts at: 3739 3739 Size: 6322 Bme1580I G_kGCm'C Cuts at: 1790 2102 2249 4383 5539 1790 Size: 312 147 2134 1156 2573 Fragments arranged by size: 2573 2134 1156 312 147 BmgBI CAC'GTC Cuts at: 1318 2059 2128 1318 Size: 741 69 5512 Fragments arranged by size: 5512 741 69 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2171 2688 2940 Size: 416 53 83 399 24 754 517 252 Cuts at: 2940 4053 5011 442 Size: 1113 958 1753 Fragments arranged by size: 1753 1113 958 754 517 416 399 252 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6322 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3631 3631 Size: 6322 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 2211 3204 3288 4982 1239 Size: 917 55 993 84 1694 2579 Fragments arranged by size: 2579 1694 993 917 84 55 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4447 5312 5610 4447 Size: 865 298 5159 Fragments arranged by size: 5159 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2132 2512 4985 882 Size: 617 633 380 2473 2219 Fragments arranged by size: 2473 2219 633 617 380 BsaAI yAC'GTr Cuts at: 493 1204 3489 4113 493 Size: 711 2285 624 2702 Fragments arranged by size: 2702 2285 711 624 BsaBI GATnn'nnATC Cuts at: 6316 6316 Size: 6322 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4129 4511 5146 275 Size: 53 83 186 936 2596 382 635 1451 Fragments arranged by size: 2596 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2862 3014 3098 3504 4755 5153 5427 5574 5985 Size: 152 84 406 1251 398 274 147 411 Cuts at: 5985 2862 Size: 3199 Fragments arranged by size: 3199 1251 411 406 398 274 152 147 84 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6097 Fragments arranged by size: 6097 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6322 BseYI C'CCAG_C Cuts at: 2281 5525 6021 2281 Size: 3244 496 2582 Fragments arranged by size: 3244 2582 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3391 3413 3559 1163 Size: 573 1655 22 146 3926 Fragments arranged by size: 3926 1655 573 146 22 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3772 4533 5561 1540 Size: 275 186 1771 761 1028 2301 Fragments arranged by size: 2301 1771 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2265 3488 4383 4468 5539 2265 Size: 1223 895 85 1071 3048 Fragments arranged by size: 3048 1223 1071 895 85 BsmI GAATG_Cn' Cuts at: 3290 3833 3926 3290 Size: 543 93 5686 Fragments arranged by size: 5686 543 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2102 2226 2249 2265 3488 3641 4383 Size: 399 312 124 23 16 1223 153 742 Cuts at: 4383 4468 5539 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1223 1071 742 399 312 153 124 85 23 16 BspEI T'CCGG_A Cuts at: 5985 5985 Size: 6322 BspHI T'CATG_A Cuts at: 2907 4211 5941 2907 Size: 1304 1730 3288 Fragments arranged by size: 3288 1730 1304 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2182 3677 844 Size: 1338 1495 3489 Fragments arranged by size: 3489 1495 1338 BsrBI CCG'CTC Cuts at: 1394 1823 4209 1394 Size: 429 2386 3507 Fragments arranged by size: 3507 2386 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3295 4817 4991 65 Size: 1183 2047 1522 174 1396 Fragments arranged by size: 2047 1522 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2862 4966 5153 1700 Size: 110 1052 2104 187 2869 Fragments arranged by size: 2869 2104 1052 187 110 BsrGI T'GTAC_A Cuts at: 96 2587 96 Size: 2491 3831 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6322 BssSI C'ACGA_G Cuts at: 3242 4382 5394 3242 Size: 1140 1012 4170 Fragments arranged by size: 4170 1140 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3462 794 Size: 2668 3654 BstBI TT'CG_AA Cuts at: 5126 5126 Size: 6322 BstEII G'GTnAC_C Cuts at: 1184 2044 1184 Size: 860 5462 BstXI CCAn_nnnn'nTGG Cuts at: 3244 3244 Size: 6322 BstYI r'GATC_y Cuts at: 1972 2344 2354 3510 3652 4019 4404 4421 5873 Size: 372 10 1156 142 367 385 17 1452 Cuts at: 5873 5959 5971 6317 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1156 385 372 367 346 142 86 17 12 10 Bsu36I CC'TnA_GG Cuts at: 2486 2486 Size: 6322 BtgI C'CryG_G Cuts at: 513 1065 2004 3433 3618 513 Size: 552 939 1429 185 3217 Fragments arranged by size: 3217 1429 939 552 185 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5583 Fragments arranged by size: 5583 683 56 BtsI GCAGTG_nn' Cuts at: 3838 4632 4652 3838 Size: 794 20 5508 Fragments arranged by size: 5508 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6322 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3109 3144 3277 3312 529 Size: 35 2545 35 133 35 3539 Fragments arranged by size: 3539 2545 133 35 35 35 DraI TTT'AAA Cuts at: 3626 3626 Size: 6322 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5329 809 Size: 507 4013 1802 Fragments arranged by size: 4013 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3769 4658 6287 Size: 54 1425 50 275 18 1939 889 1629 Cuts at: 6287 8 Size: 43 Fragments arranged by size: 1939 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3769 1537 Size: 275 1957 4090 Fragments arranged by size: 4090 1957 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2760 2989 4252 1353 Size: 1407 229 1263 3423 Fragments arranged by size: 3423 1407 1263 229 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4909 5283 5429 387 Size: 1064 138 3320 374 146 1280 Fragments arranged by size: 3320 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2211 2620 2784 2788 3040 3204 Size: 393 524 55 409 164 4 252 164 Cuts at: 3204 3288 3460 4385 4982 5769 6037 1239 Size: 84 172 925 597 787 268 1524 Fragments arranged by size: 1524 925 787 597 524 409 393 268 252 172 164 164 84 55 4 EcoNI CCTnn'n_nnAGG Cuts at: 3605 3605 Size: 6322 EcoO109I rG'GnC_Cy Cuts at: 2099 2113 2171 2180 2230 2429 2099 Size: 14 58 9 50 199 5992 Fragments arranged by size: 5992 199 58 50 14 9 EcoRI G'AATT_C Cuts at: 3174 3631 4009 3174 Size: 457 378 5487 Fragments arranged by size: 5487 457 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3490 3490 Size: 6322 FspI TGC'GCA Cuts at: 4828 4828 Size: 6322 HaeII r_GCGC'y Cuts at: 1678 2979 5469 1678 Size: 1301 2490 2531 Fragments arranged by size: 2531 2490 1301 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2551 2553 2583 2585 4963 Size: 32 135 32 979 2 30 2 2378 Cuts at: 4963 4995 5037 5069 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2378 979 135 42 32 32 32 32 30 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 2275 2316 3666 3912 669 Size: 988 281 337 41 1350 246 3079 Fragments arranged by size: 3079 1350 988 337 281 246 41 HindIII A'AGCT_T Cuts at: 748 3219 748 Size: 2471 3851 HpaI GTT'AAC Cuts at: 3912 3912 Size: 6322 KpnI G_GTAC'C Cuts at: 3647 3647 Size: 6322 MfeI C'AATT_G Cuts at: 3921 3921 Size: 6322 MluI A'CGCG_T Cuts at: 6198 6198 Size: 6322 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2187 3068 3103 3187 3597 5436 5620 1305 Size: 882 881 35 84 410 1839 184 2007 Fragments arranged by size: 2007 1839 882 881 410 184 84 35 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4500 Fragments arranged by size: 4500 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2529 2583 3115 4280 4639 4798 518 Size: 648 1363 54 532 1165 359 159 2042 Fragments arranged by size: 2042 1363 1165 648 532 359 159 54 MspA1I CmG'CkG Cuts at: 1606 2752 3592 3738 4415 5563 5808 1606 Size: 1146 840 146 677 1148 245 2120 Fragments arranged by size: 2120 1148 1146 840 677 245 146 NcoI C'CATG_G Cuts at: 513 1065 2004 3618 513 Size: 552 939 1614 3217 Fragments arranged by size: 3217 1614 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6322 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6322 NotI GC'GGCC_GC Cuts at: 3769 3769 Size: 6322 NspI r_CATG'y Cuts at: 1132 1229 3580 3680 5225 1132 Size: 97 2351 100 1545 2229 Fragments arranged by size: 2351 2229 1545 100 97 PasI CC'CwG_GG Cuts at: 1348 2226 2350 2433 1348 Size: 878 124 83 5237 Fragments arranged by size: 5237 878 124 83 PciI A'CATG_T Cuts at: 3576 5221 3576 Size: 1645 4677 PflMI CCAn_nnn'nTGG Cuts at: 1108 2288 1108 Size: 1180 5142 PfoI T'CCnGG_A Cuts at: 2232 3603 2232 Size: 1371 4951 PmeI GTTT'AAAC Cuts at: 3626 3626 Size: 6322 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2141 2173 4391 4423 5904 5936 2141 Size: 32 2218 32 1481 32 2527 Fragments arranged by size: 2527 2218 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2171 2230 2171 Size: 59 6263 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6322 PsiI TTA'TAA Cuts at: 3892 3892 Size: 6322 PspOMI G'GGCC_C Cuts at: 2098 2098 Size: 6322 PstI C_TGCA'G Cuts at: 830 2179 3674 830 Size: 1349 1495 3478 Fragments arranged by size: 3478 1495 1349 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6322 SalI G'TCGA_C Cuts at: 1936 3664 1936 Size: 1728 4594 SbfI CC_TGCA'GG Cuts at: 2179 3674 2179 Size: 1495 4827 ScaI AGT'ACT Cuts at: 4570 6282 4570 Size: 1712 4610 SfcI C'TryA_G Cuts at: 826 1045 2119 2175 3448 3670 4805 5486 5677 Size: 219 1074 56 1273 222 1135 681 191 Cuts at: 5677 826 Size: 1471 Fragments arranged by size: 1471 1273 1135 1074 681 222 219 191 56 SfiI GGCCn_nnn'nGGCC Cuts at: 2097 2097 Size: 6322 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6322 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6322 SmaI CCC'GGG Cuts at: 1872 2078 3649 1872 Size: 206 1571 4545 Fragments arranged by size: 4545 1571 206 SmlI C'TyrA_G Cuts at: 820 1017 1943 4426 5327 5589 820 Size: 197 926 2483 901 262 1553 Fragments arranged by size: 2483 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4113 493 Size: 3620 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6322 SphI G_CATG'C Cuts at: 1132 3680 1132 Size: 2548 3774 SspI AAT'ATT Cuts at: 5 52 4246 5 Size: 47 4194 2081 Fragments arranged by size: 4194 2081 47 StuI AGG'CCT Cuts at: 1517 3389 3557 1517 Size: 1872 168 4282 Fragments arranged by size: 4282 1872 168 StyI C'CwwG_G Cuts at: 513 1065 2004 3618 3761 513 Size: 552 939 1614 143 3074 Fragments arranged by size: 3074 1614 939 552 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4349 4366 4519 4704 5149 325 Size: 1255 2769 17 153 185 445 1498 Fragments arranged by size: 2769 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2304 2587 4568 Size: 274 80 33 51 1443 327 283 1981 Cuts at: 4568 6280 96 Size: 1712 138 Fragments arranged by size: 1981 1712 1443 327 283 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3312 3344 3386 3418 6282 6314 3312 Size: 32 42 32 2864 32 3320 Fragments arranged by size: 3320 2864 42 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3084 1316 Size: 1768 4554 XbaI T'CTAG_A Cuts at: 3658 3658 Size: 6322 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6322 XmaI C'CCGG_G Cuts at: 1870 2076 3647 1870 Size: 206 1571 4545 Fragments arranged by size: 4545 1571 206 XmnI GAAnn'nnTTC Cuts at: 2717 2801 2885 3053 3221 3389 3473 3557 4451 Size: 84 84 168 168 168 84 84 894 Cuts at: 4451 2717 Size: 4588 Fragments arranged by size: 4588 894 168 168 168 84 84 84 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4130 276 Size: 53 83 186 936 2596 2468 Fragments arranged by size: 2596 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI XcmI