Restriction map for
pFN21AB8337
(Linear) (Six-base) MAPSORT of: KIBB8337.seq Check: 5045 from: 1 to: 1617
KIBB8337.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 615 779 783 1035 1455 1617
Size: 615 164 4 252 420 162
Fragments arranged by size:
615 420 252 164 162 4
AflIII A'CryG_T
Cuts at: 0 1571 1617
Size: 1571 46
AgeI A'CCGG_T
Cuts at: 0 857 1617
Size: 857 760
AleI CACnn'nnGTG
Cuts at: 0 524 1617
Size: 524 1093
AlwNI CAG_nnn'CTG
Cuts at: 0 701 1422 1617
Size: 701 721 195
Fragments arranged by size:
721 701 195
ApaI G_GGCC'C
Cuts at: 0 97 1617
Size: 97 1520
ApoI r'AATT_y
Cuts at: 0 665 1169 1617
Size: 665 504 448
Fragments arranged by size:
665 504 448
AvaI C'yCGr_G
Cuts at: 0 71 1617
Size: 71 1546
BamHI G'GATC_C
Cuts at: 0 1505 1617
Size: 1505 112
BanI G'GyrC_C
Cuts at: 0 241 1617
Size: 241 1376
BanII G_rGCy'C
Cuts at: 0 97 221 1617
Size: 97 124 1396
Fragments arranged by size:
1396 124 97
BbsI GAAGACnn'nnnn_
Cuts at: 0 883 1617
Size: 883 734
BclI T'GATC_A
Cuts at: 0 507 1617
Size: 507 1110
BglI GCCn_nnn'nGGC
Cuts at: 0 92 1617
Size: 92 1525
Bme1580I G_kGCm'C
Cuts at: 0 97 244 1617
Size: 97 147 1373
Fragments arranged by size:
1373 147 97
BmgBI CAC'GTC
Cuts at: 0 54 123 1617
Size: 54 69 1494
Fragments arranged by size:
1494 69 54
BmrI ACTGGGnnnn_n'
Cuts at: 0 166 683 935 1617
Size: 166 517 252 682
Fragments arranged by size:
682 517 252 166
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 206 1199 1283 1617
Size: 151 55 993 84 334
Fragments arranged by size:
993 334 151 84 55
BsaI GGTCTCn'nnnn_
Cuts at: 0 127 507 1617
Size: 127 380 1110
Fragments arranged by size:
1110 380 127
BsaAI yAC'GTr
Cuts at: 0 1484 1617
Size: 1484 133
BsaWI w'CCGG_w
Cuts at: 0 857 1009 1093 1499 1617
Size: 857 152 84 406 118
Fragments arranged by size:
857 406 152 118 84
BseYI C'CCAG_C
Cuts at: 0 276 1617
Size: 276 1341
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1386 1408 1554 1617
Size: 1386 22 146 63
Fragments arranged by size:
1386 146 63 22
BsiHKAI G_wGCw'C
Cuts at: 0 260 1483 1617
Size: 260 1223 134
Fragments arranged by size:
1223 260 134
BsmI GAATG_Cn'
Cuts at: 0 1285 1617
Size: 1285 332
Bsp1286I G_dGCh'C
Cuts at: 0 97 221 244 260 1483 1617
Size: 97 124 23 16 1223 134
Fragments arranged by size:
1223 134 124 97 23 16
BspHI T'CATG_A
Cuts at: 0 902 1617
Size: 902 715
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 177 1617
Size: 177 1440
BsrDI GCAATG_nn'
Cuts at: 0 1290 1617
Size: 1290 327
BsrFI r'CCGG_y
Cuts at: 0 857 1617
Size: 857 760
BsrGI T'GTAC_A
Cuts at: 0 582 1617
Size: 582 1035
BssSI C'ACGA_G
Cuts at: 0 1237 1617
Size: 1237 380
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1457 1617
Size: 1457 160
BstEII G'GTnAC_C
Cuts at: 0 39 1617
Size: 39 1578
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1239 1617
Size: 1239 378
BstYI r'GATC_y
Cuts at: 0 339 349 1505 1617
Size: 339 10 1156 112
Fragments arranged by size:
1156 339 112 10
Bsu36I CC'TnA_GG
Cuts at: 0 481 1617
Size: 481 1136
BtgI C'CryG_G
Cuts at: 0 1428 1617
Size: 1428 189
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1104 1139 1272 1307 1617
Size: 1104 35 133 35 310
Fragments arranged by size:
1104 310 133 35 35
EarI CTCTTCn'nnn_
Cuts at: 0 755 984 1617
Size: 755 229 633
Fragments arranged by size:
755 633 229
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 206 615 779 783 1035 1199 1283
Size: 151 55 409 164 4 252 164 84
Cuts at: 1283 1455 1617
Size: 172 162
Fragments arranged by size:
409 252 172 164 164 162 151 84
55 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1600 1617
Size: 1600 17
EcoO109I rG'GnC_Cy
Cuts at: 0 94 108 166 175 225 424 1617
Size: 94 14 58 9 50 199 1193
Fragments arranged by size:
1193 199 94 58 50 14 9
EcoRI G'AATT_C
Cuts at: 0 1169 1617
Size: 1169 448
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1485 1617
Size: 1485 132
HaeII r_GCGC'y
Cuts at: 0 974 1617
Size: 974 643
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 546 548 578 580 1617
Size: 546 2 30 2 1037
Fragments arranged by size:
1037 546 30 2 2
HincII GTy'rAC
Cuts at: 0 270 311 1617
Size: 270 41 1306
Fragments arranged by size:
1306 270 41
HindIII A'AGCT_T
Cuts at: 0 1214 1617
Size: 1214 403
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 1063 1098 1182 1592 1617
Size: 182 881 35 84 410 25
Fragments arranged by size:
881 410 182 84 35 25
MslI CAynn'nnrTG
Cuts at: 0 524 578 1110 1617
Size: 524 54 532 507
Fragments arranged by size:
532 524 507 54
MspA1I CmG'CkG
Cuts at: 0 747 1587 1617
Size: 747 840 30
Fragments arranged by size:
840 747 30
NspI r_CATG'y
Cuts at: 0 1575 1617
Size: 1575 42
PasI CC'CwG_GG
Cuts at: 0 221 345 428 1617
Size: 221 124 83 1189
Fragments arranged by size:
1189 221 124 83
PciI A'CATG_T
Cuts at: 0 1571 1617
Size: 1571 46
PflMI CCAn_nnn'nTGG
Cuts at: 0 283 1617
Size: 283 1334
PfoI T'CCnGG_A
Cuts at: 0 227 1598 1617
Size: 227 1371 19
Fragments arranged by size:
1371 227 19
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 136 168 1617
Size: 136 32 1449
Fragments arranged by size:
1449 136 32
PpuMI rG'GwC_Cy
Cuts at: 0 166 225 1617
Size: 166 59 1392
Fragments arranged by size:
1392 166 59
PspOMI G'GGCC_C
Cuts at: 0 93 1617
Size: 93 1524
PstI C_TGCA'G
Cuts at: 0 174 1617
Size: 174 1443
SbfI CC_TGCA'GG
Cuts at: 0 174 1617
Size: 174 1443
SfcI C'TryA_G
Cuts at: 0 114 170 1443 1617
Size: 114 56 1273 174
Fragments arranged by size:
1273 174 114 56
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 92 1617
Size: 92 1525
SmaI CCC'GGG
Cuts at: 0 73 1617
Size: 73 1544
StuI AGG'CCT
Cuts at: 0 1384 1552 1617
Size: 1384 168 65
Fragments arranged by size:
1384 168 65
TatI w'GTAC_w
Cuts at: 0 299 582 1617
Size: 299 283 1035
Fragments arranged by size:
1035 299 283
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1307 1339 1381 1413 1617
Size: 1307 32 42 32 204
Fragments arranged by size:
1307 204 42 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1079 1617
Size: 1079 538
XmaI C'CCGG_G
Cuts at: 0 71 1617
Size: 71 1546
XmnI GAAnn'nnTTC
Cuts at: 0 712 796 880 1048 1216 1384 1468 1552
Size: 712 84 84 168 168 168 84 84
Cuts at: 1552 1617
Size: 65
Fragments arranged by size:
712 168 168 168 84 84 84 84
65
Enzymes that do cut:
AcuI AflIII AgeI AleI AlwNI ApaI ApoI AvaI
BamHI BanI BanII BbsI BclI BglI Bme1580I BmgBI
BmrI BpmI BsaI BsaAI BsaWI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI CspCI EarI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII
HindIII MmeI MslI MspA1I NspI PasI PciI PflMI
PfoI PpiI PpuMI PspOMI PstI SbfI SfcI SfiI
SmaI StuI TatI TstI Tth111I XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AhdI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BbeI BbvCI BcgI BciVI BfrBI BglII BlpI BmtI
BplI Bpu10I BpuEI BsaBI BsaHI BsaXI BseRI BsiEI
BsiWI BsmBI BspEI BsrBI BssHII BstBI BstZ17I BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EagI EciI
EcoICRI EcoRV FseI FspI FspAI HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PmeI PmlI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI ScaI SexAI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StyI SwaI
TaqII XbaI XcmI XhoI ZraI