Restriction map for
pFN21AB8335
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI ApaI
AscI AseI AsnI Asp718I AspEI
AvrII BalI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BspLU11I BsrDI Bst1107I BstBI BstZ17I
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco52I EcoNI EcoRI EcoRV EcoT22I
FbaI FseI HgaI Hin1I HincII
HindII HpaI KasI KpnI Ksp632I
KspI MaeII MamI MluI MluNI
MroI MscI MseI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI TfiI Tru9I Van91I
XbaI XhoI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 412 GTAGAC GT^[AC][GT]AC
AcsI 538 GAATTT [GA]^AATT[CT]
Alw44I 319 GTGCAC G^TGCAC
Aor51HI 1208 AGCGCT AGC^GCT
ApaLI 319 GTGCAC G^TGCAC
ApoI 538 GAATTT [GA]^AATT[CT]
AspHI 319 GTGCAC G[AT]GC[AT]^C
AspI 394 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 25 CCCGGG C^[CT]CG[GA]G
AviII 56 TGCGCA TGC^GCA
BamHI 484 GGATCC G^GATCC
BbsI 391 GAAGAC GAAGAC(2/6)
BfaI 1150 CTAG C^TAG
BpuAI 391 GAAGAC GAAGAC(2/6)
BseRI 217 GAGGAG GAGGAG(10/8)
BsgI 1193 GTGCAG GTGCAG(16/14)
BsiHKAI 319 GTGCAC G[AT]GC[AT]^C
BsoBI 25 CCCGGG C^[CT]CG[GA]G
Bsp1407I 797 TGTACA T^GTACA
BsrFI 686 GCCGGC [GA]^CCGG[CT]
BsrGI 797 TGTACA T^GTACA
BssHII 829 GCGCGC G^CGCGC
BssSI 325 CACGAG CACGAG(-5/-1)
BstEII 186 GGTCACC G^GT[ACGT]ACC
BstPI 186 GGTCACC G^GT[ACGT]ACC
Cfr10I 686 GCCGGC [GA]^CCGG[CT]
DsaI 192 CCATGG C^C[GA][CT]GG
Eco47III 1208 AGCGCT AGC^GCT
EcoO65I 186 GGTCACC G^GT[ACGT]ACC
FspI 56 TGCGCA TGC^GCA
HaeII 1208 AGCGCT [GA]GCGC^[CT]
HindIII 871 AAGCTT A^AGCTT
HinfI 382 GAGTC G^A[ACGT]TC
MaeI 1150 CTAG C^TAG
MfeI 1052 CAATTG C^AATTG
MunI 1052 CAATTG C^AATTG
NaeI 686 GCCGGC GCC^GGC
NcoI 192 CCATGG C^CATGG
NgoMIV 686 GCCGGC G^CCGGC
PflFI 394 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 382 GAGTC GAGTC(4/5)
PshAI 1284 GACTTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 907 CAGCTG CAG^CTG
ScaI 567 AGTACT AGT^ACT
SexAI 983 ACCTGGT A^CC[AT]GGT
SmaI 25 CCCGGG CCC^GGG
SspBI 797 TGTACA T^GTACA
StuI 329 AGGCCT AGG^CCT
Tth111I 394 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 25 CCCGGG C^CCGGG
XmaI 25 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AfaI 568 GTAC GT^AC
798 GTAC
AocI 472 CCTGAGG CC^T[ACGT]AGG
817 CCTGAGG
Asp700I 249 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
294 GAACACCTTC
BanII 81 GGGCTC G[GA]GC[CT]^C
166 GAGCCC
BsaI 5 GAGACC GGTCTC(1/5)
1129 GAGACC
BsrBI 1015 GAGCGG CCGCTC(-3/-3)
1168 GAGCGG
BsrI 210 ACTGG ACTGG(1/-1)
1265 ACTGG
BstF5I 138 CATCC GGATG(2/0)
1143 GGATG
BstXI 305 CCATCTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1003 CCAGAAATCTGG
BstYI 484 GGATCC [GA]^GATC[CT]
998 AGATCC
Bsu36I 472 CCTGAGG CC^T[ACGT]AGG
817 CCTGAGG
Eco81I 472 CCTGAGG CC^T[ACGT]AGG
817 CCTGAGG
EcoT14I 192 CCATGG C^C[AT][AT]GG
854 CCAAGG
FokI 138 CATCC GGATG(9/13)
1143 GGATG
HphI 1306 GGTGA GGTGA(8/7)
188 TCACC
MaeIII 187 GTCAC ^GT[ACGT]AC
1307 GTGAC
MboII 391 GAAGA GAAGA(8/7)
362 TCTTC
MflI 484 GGATCC [GA]^GATC[CT]
998 AGATCC
MslI 306 CATCTACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
312 CATGGTGGTG
NspI 128 ACATGC [GA]CATG^[CT]
1074 GCATGT
RsaI 568 GTAC GT^AC
798 GTAC
SfaNI 145 GATGC GCATC(5/9)
1220 GATGC
SmlI 615 CTTGAG C^T[CT][GA]AG
868 CTCAAG
StyI 192 CCATGG C^C[AT][AT]GG
854 CCAAGG
TaqI 143 TCGA T^CGA
716 TCGA
Tsp45I 187 GTCAC ^GT[GC]AC
1307 GTGAC
Tsp509I 539 AATT ^AATT
1053 AATT
TthHB8I 143 TCGA T^CGA
716 TCGA
XcmI 305 CCATCTACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
450 CCAGAGTGGCTGTGG
XhoII 484 GGATCC [GA]^GATC[CT]
998 AGATCC
XmnI 249 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
294 GAACACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AvaII BbvI BcnI BlpI BmyI
BpmI Bpu1102I BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BssKI
BstAPI BstNI BstUI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII FauI Fnu4HI HaeIII
HapII HhaI HinP1I HpaII ItaI
MboI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PpuMI PstI Sau3AI Sau96I
ScrFI SfcI TseI TspRI