(Circular) (Six-base) MAPSORT of: pFN21AB8335.seq Check: 4589 from: 1 to: 6025 pFN21AB8335 6025 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3835 278 Size: 53 83 186 936 2299 2468 Fragments arranged by size: 2468 2299 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2418 3368 1937 Size: 481 950 4594 Fragments arranged by size: 4594 950 481 Acc65I G'GTAC_C Cuts at: 3346 3346 Size: 6025 AclI AA'CG_TT Cuts at: 1563 4152 4525 1563 Size: 2589 373 3063 Fragments arranged by size: 3063 2589 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2061 2579 4088 5472 5740 1632 Size: 429 518 1509 1384 268 1917 Fragments arranged by size: 1917 1509 1384 518 429 268 AfeI AGC'GCT Cuts at: 3215 3215 Size: 6025 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5828 AflIII A'CryG_T Cuts at: 1203 4924 5901 1203 Size: 3721 977 1327 Fragments arranged by size: 3721 1327 977 AgeI A'CCGG_T Cuts at: 4856 4856 Size: 6025 AhdI GACnn_n'nnGTC Cuts at: 4754 4754 Size: 6025 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6025 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2525 3075 1184 Size: 1341 550 4134 Fragments arranged by size: 4134 1341 550 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2348 2380 2348 Size: 32 5993 AlwNI CAG_nnn'CTG Cuts at: 2185 2518 3106 3768 5340 2185 Size: 333 588 662 1572 2870 Fragments arranged by size: 2870 1572 662 588 333 ApaLI G'TGCA_C Cuts at: 2324 4082 5238 2324 Size: 1758 1156 3111 Fragments arranged by size: 3111 1758 1156 ApoI r'AATT_y Cuts at: 1493 2543 3334 3560 3712 5821 1493 Size: 1050 791 226 152 2109 1697 Fragments arranged by size: 2109 1697 1050 791 226 152 AseI AT'TA_AT Cuts at: 160 1032 4579 160 Size: 872 3547 1606 Fragments arranged by size: 3547 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6025 AvaI C'yCGr_G Cuts at: 1870 1943 2030 3350 1870 Size: 73 87 1320 4545 Fragments arranged by size: 4545 1320 87 73 BamHI G'GATC_C Cuts at: 2489 3355 3722 2489 Size: 866 367 4792 Fragments arranged by size: 4792 866 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3346 4801 618 Size: 325 217 627 1559 1455 1842 Fragments arranged by size: 1842 1559 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2090 2175 3344 1391 Size: 699 85 1169 4072 Fragments arranged by size: 4072 1169 699 85 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2403 928 Size: 971 504 4550 Fragments arranged by size: 4550 971 504 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4216 4250 1903 Size: 34 2279 34 3678 Fragments arranged by size: 3678 2279 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3917 5127 3917 Size: 1210 4815 BclI T'GATC_A Cuts at: 1548 1548 Size: 6025 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4636 136 Size: 107 122 71 631 605 2964 1525 Fragments arranged by size: 2964 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6020 6020 Size: 6025 BlpI GC'TnA_GC Cuts at: 2633 2768 2816 3442 2633 Size: 135 48 626 5216 Fragments arranged by size: 5216 626 135 48 Bme1580I G_kGCm'C Cuts at: 1790 2328 4086 5242 1790 Size: 538 1758 1156 2573 Fragments arranged by size: 2573 1758 1156 538 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6025 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3280 3756 4714 Size: 416 53 83 399 24 1863 476 958 Cuts at: 4714 442 Size: 1753 Fragments arranged by size: 1863 1753 958 476 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6025 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2104 2104 Size: 6025 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2141 2693 2707 3047 3059 3153 4685 1239 Size: 902 552 14 340 12 94 1532 2579 Fragments arranged by size: 2579 1532 902 552 340 94 14 12 Bpu10I CC'TnA_GC Cuts at: 2829 2829 Size: 6025 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2641 2858 4150 5015 5313 2641 Size: 217 1292 865 298 3353 Fragments arranged by size: 3353 1292 865 298 217 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2004 3128 4688 882 Size: 617 505 1124 1560 2219 Fragments arranged by size: 2219 1560 1124 617 505 BsaAI yAC'GTr Cuts at: 493 1204 3816 493 Size: 711 2612 2702 Fragments arranged by size: 2702 2612 711 BsaBI GATnn'nnATC Cuts at: 6019 6019 Size: 6025 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3832 4214 4849 275 Size: 53 83 186 936 2299 382 635 1451 Fragments arranged by size: 2299 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4458 4856 5130 5277 5688 4458 Size: 398 274 147 411 4795 Fragments arranged by size: 4795 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2321 2348 2351 2378 2959 Size: 30 165 30 918 27 3 27 581 Cuts at: 2959 2989 1178 Size: 30 4214 Fragments arranged by size: 4214 918 581 165 30 30 30 27 27 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2237 1186 Size: 1051 4974 BseYI C'CCAG_C Cuts at: 2970 5228 5724 2970 Size: 2258 496 3271 Fragments arranged by size: 3271 2258 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3219 1163 Size: 573 1483 3969 Fragments arranged by size: 3969 1483 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3475 4236 5264 1540 Size: 275 186 1474 761 1028 2301 Fragments arranged by size: 2301 1474 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2328 4086 4171 5242 2328 Size: 1758 85 1071 3111 Fragments arranged by size: 3111 1758 1071 85 BsmI GAATG_Cn' Cuts at: 3536 3629 3536 Size: 93 5932 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2090 2175 2328 3344 4086 4171 5242 Size: 399 300 85 153 1016 742 85 1071 Cuts at: 5242 1391 Size: 2174 Fragments arranged by size: 2174 1071 1016 742 399 300 153 85 85 BspEI T'CCGG_A Cuts at: 5688 5688 Size: 6025 BspHI T'CATG_A Cuts at: 3914 5644 3914 Size: 1730 4295 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2655 2817 3114 3380 844 Size: 1811 162 297 266 3489 Fragments arranged by size: 3489 1811 297 266 162 BsrBI CCG'CTC Cuts at: 1394 1823 3022 3175 3912 1394 Size: 429 1199 153 737 3507 Fragments arranged by size: 3507 1199 737 429 153 BsrDI GCAATG_nn' Cuts at: 65 1248 4520 4694 65 Size: 1183 3272 174 1396 Fragments arranged by size: 3272 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2691 4669 4856 1700 Size: 110 881 1978 187 2869 Fragments arranged by size: 2869 1978 881 187 110 BsrGI T'GTAC_A Cuts at: 96 2802 96 Size: 2706 3319 BssHII G'CGCG_C Cuts at: 1925 2834 1925 Size: 909 5116 BssSI C'ACGA_G Cuts at: 2330 4085 5097 2330 Size: 1755 1012 3258 Fragments arranged by size: 3258 1755 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2518 3106 3255 794 Size: 1724 588 149 3564 Fragments arranged by size: 3564 1724 588 149 BstBI TT'CG_AA Cuts at: 4829 4829 Size: 6025 BstEII G'GTnAC_C Cuts at: 1184 2191 1184 Size: 1007 5018 BstXI CCAn_nnnn'nTGG Cuts at: 2317 3015 2317 Size: 698 5327 BstYI r'GATC_y Cuts at: 1972 2489 3003 3355 3722 4107 4124 5576 5662 Size: 517 514 352 367 385 17 1452 86 Cuts at: 5662 5674 6020 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 517 514 385 367 352 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2478 2823 2478 Size: 345 5680 BtgI C'CryG_G Cuts at: 513 1065 2004 2197 513 Size: 552 939 193 4341 Fragments arranged by size: 4341 939 552 193 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5286 Fragments arranged by size: 5286 683 56 BtsI GCAGTG_nn' Cuts at: 2241 2900 3168 3202 3541 4335 4355 2241 Size: 659 268 34 339 794 20 3911 Fragments arranged by size: 3911 794 659 339 268 34 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6025 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2199 2234 529 Size: 35 1635 35 4320 Fragments arranged by size: 4320 1635 35 35 DraI TTT'AAA Cuts at: 3329 3329 Size: 6025 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5032 809 Size: 507 3716 1802 Fragments arranged by size: 3716 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3472 4361 5990 Size: 54 1425 50 275 18 1642 889 1629 Cuts at: 5990 8 Size: 43 Fragments arranged by size: 1642 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3472 1537 Size: 275 1660 4090 Fragments arranged by size: 4090 1660 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3955 1353 Size: 2602 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4612 4986 5132 387 Size: 1064 138 3023 374 146 1280 Fragments arranged by size: 3023 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2061 2141 2579 2693 2707 3047 3059 Size: 393 429 80 438 114 14 340 12 Cuts at: 3059 3153 4088 4685 5472 5740 1239 Size: 94 935 597 787 268 1524 Fragments arranged by size: 1524 935 787 597 438 429 393 340 268 114 94 80 14 12 EcoO109I rG'GnC_Cy Cuts at: 2206 2563 2986 3103 2206 Size: 357 423 117 5128 Fragments arranged by size: 5128 423 357 117 EcoRI G'AATT_C Cuts at: 3334 3712 3334 Size: 378 5647 FspI TGC'GCA Cuts at: 2063 4531 2063 Size: 2468 3557 HaeII r_GCGC'y Cuts at: 1678 3217 5172 1678 Size: 1539 1955 2531 Fragments arranged by size: 2531 1955 1539 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2591 2623 2856 2888 4666 Size: 32 135 32 1019 32 233 32 1778 Cuts at: 4666 4698 4740 4772 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1778 1019 233 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3369 3615 669 Size: 988 281 1431 246 3079 Fragments arranged by size: 3079 1431 988 281 246 HindIII A'AGCT_T Cuts at: 748 2876 748 Size: 2128 3897 HpaI GTT'AAC Cuts at: 3615 3615 Size: 6025 KpnI G_GTAC'C Cuts at: 3350 3350 Size: 6025 MfeI C'AATT_G Cuts at: 3057 3624 3057 Size: 567 5458 MluI A'CGCG_T Cuts at: 5901 5901 Size: 6025 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2757 2976 5139 5323 1305 Size: 1452 219 2163 184 2007 Fragments arranged by size: 2163 2007 1452 219 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4203 Fragments arranged by size: 4203 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2315 2321 3983 4342 4501 518 Size: 648 1149 6 1662 359 159 2042 Fragments arranged by size: 2042 1662 1149 648 359 159 6 MspA1I CmG'CkG Cuts at: 1606 2107 2914 3183 3441 4118 5266 5511 1606 Size: 501 807 269 258 677 1148 245 2120 Fragments arranged by size: 2120 1148 807 677 501 269 258 245 NaeI GCC'GGC Cuts at: 2693 2693 Size: 6025 NcoI C'CATG_G Cuts at: 513 1065 2004 2197 513 Size: 552 939 193 4341 Fragments arranged by size: 4341 939 552 193 NdeI CA'TA_TG Cuts at: 387 387 Size: 6025 NgoMIV G'CCGG_C Cuts at: 2691 2691 Size: 6025 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6025 NotI GC'GGCC_GC Cuts at: 3472 3472 Size: 6025 NspI r_CATG'y Cuts at: 1132 1229 2137 3083 3383 4928 1132 Size: 97 908 946 300 1545 2229 Fragments arranged by size: 2229 1545 946 908 300 97 PasI CC'CwG_GG Cuts at: 1348 3307 1348 Size: 1959 4066 PciI A'CATG_T Cuts at: 4924 4924 Size: 6025 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6025 PmeI GTTT'AAAC Cuts at: 3329 3329 Size: 6025 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2348 2380 4094 4126 5607 5639 2348 Size: 32 1714 32 1481 32 2734 Fragments arranged by size: 2734 1714 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2206 2986 3103 2206 Size: 780 117 5128 Fragments arranged by size: 5128 780 117 PshAI GACnn'nnGTC Cuts at: 1613 3293 1613 Size: 1680 4345 PsiI TTA'TAA Cuts at: 3595 3595 Size: 6025 PstI C_TGCA'G Cuts at: 830 2276 2523 2715 3117 3197 3251 3377 830 Size: 1446 247 192 402 80 54 126 3478 Fragments arranged by size: 3478 1446 402 247 192 126 80 54 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6025 PvuII CAG'CTG Cuts at: 2914 2914 Size: 6025 SalI G'TCGA_C Cuts at: 1936 3367 1936 Size: 1431 4594 SbfI CC_TGCA'GG Cuts at: 3377 3377 Size: 6025 ScaI AGT'ACT Cuts at: 2574 4273 5985 2574 Size: 1699 1712 2614 Fragments arranged by size: 2614 1712 1699 SexAI A'CCwGG_T Cuts at: 2988 2988 Size: 6025 SfcI C'TryA_G Cuts at: 826 1045 2272 2519 2711 3113 3139 3193 3247 Size: 219 1227 247 192 402 26 54 54 Cuts at: 3247 3373 4508 5189 5380 826 Size: 126 1135 681 191 1471 Fragments arranged by size: 1471 1227 1135 681 402 247 219 192 191 126 54 54 26 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6025 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6025 SmaI CCC'GGG Cuts at: 1872 2032 3352 1872 Size: 160 1320 4545 Fragments arranged by size: 4545 1320 160 SmlI C'TyrA_G Cuts at: 820 1017 1943 2620 2873 4129 5030 5292 820 Size: 197 926 677 253 1256 901 262 1553 Fragments arranged by size: 1553 1256 926 901 677 262 253 197 SnaBI TAC'GTA Cuts at: 493 3816 493 Size: 3323 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6025 SphI G_CATG'C Cuts at: 1132 3383 1132 Size: 2251 3774 SspI AAT'ATT Cuts at: 5 52 3949 5 Size: 47 3897 2081 Fragments arranged by size: 3897 2081 47 StuI AGG'CCT Cuts at: 1517 2336 1517 Size: 819 5206 StyI C'CwwG_G Cuts at: 513 1065 2004 2197 2859 3464 513 Size: 552 939 193 662 605 3074 Fragments arranged by size: 3074 939 662 605 552 193 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2210 4052 4069 4222 4407 4852 325 Size: 1255 630 1842 17 153 185 445 1498 Fragments arranged by size: 1842 1498 1255 630 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2572 2802 4271 Size: 274 80 33 51 1443 595 230 1469 Cuts at: 4271 5983 96 Size: 1712 138 Fragments arranged by size: 1712 1469 1443 595 274 230 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2321 2353 5985 6017 2321 Size: 32 3632 32 2329 Fragments arranged by size: 3632 2329 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2402 1316 Size: 1086 4939 XbaI T'CTAG_A Cuts at: 3361 3361 Size: 6025 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2317 2462 2011 Size: 306 145 5574 Fragments arranged by size: 5574 306 145 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6025 XmaI C'CCGG_G Cuts at: 1870 2030 3350 1870 Size: 160 1320 4545 Fragments arranged by size: 4545 1320 160 XmnI GAAnn'nnTTC Cuts at: 2258 2303 4154 2258 Size: 45 1851 4129 Fragments arranged by size: 4129 1851 45 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3833 276 Size: 53 83 186 936 2299 2468 Fragments arranged by size: 2468 2299 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI