Restriction map for
pFN21AB8334
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# NO CUT ENZYMES ( 181 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AfaI AflII
AflIII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BbvI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmFI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BsrI BssHII Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstUI BstXI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI Fnu4HI FokI FseI FspI
HaeII HhaI HinP1I HincII HindII
HindIII HpaI ItaI KasI KpnI
KspI MaeIII MamI MfeI MluI
MluNI MroI MscI MseI MslI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I Van91I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AcyI 13 GACGCC G[GA]^CG[CT]C
AlwI 60 GATCC GGATC(4/5)
AocI 547 CCTGAGG CC^T[ACGT]AGG
AspHI 41 GTGCTC G[AT]GC[AT]^C
AvaI 423 CCCGGG C^[CT]CG[GA]G
BbsI 229 GAAGAC GAAGAC(2/6)
BfaI 181 CTAG C^TAG
BglII 460 AGATCT A^GATCT
BpmI 310 CTCCAG CTGGAG(16/14)
BpuAI 229 GAAGAC GAAGAC(2/6)
BsaHI 13 GACGCC G[GA]^CG[CT]C
BsaI 27 GAGACC GGTCTC(1/5)
BsiHKAI 41 GTGCTC G[AT]GC[AT]^C
BsmBI 8 GAGACG CGTCTC(1/5)
BsoBI 423 CCCGGG C^[CT]CG[GA]G
BssSI 454 CACGAG CACGAG(-5/-1)
Bsu36I 547 CCTGAGG CC^T[ACGT]AGG
Eco81I 547 CCTGAGG CC^T[ACGT]AGG
EcoNI 17 CCTTTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HapII 424 CCGG C^CGG
HgaI 13 GACGC GACGC(5/10)
Hin1I 13 GACGCC G[GA]^CG[CT]C
HpaII 424 CCGG C^CGG
HphI 522 GGTGA GGTGA(8/7)
MaeI 181 CTAG C^TAG
MaeII 285 ACGT A^CGT
MspA1I 517 CAGCTG C[AC]G^C[GT]G
MspI 424 CCGG C^CGG
MwoI 336 GCCCAAGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 483 ACATGC [GA]CATG^[CT]
PvuII 517 CAGCTG CAG^CTG
SfaNI 385 GCATC GCATC(5/9)
SmaI 423 CCCGGG CCC^GGG
StuI 68 AGGCCT AGG^CCT
TaqI 74 TCGA T^CGA
TthHB8I 74 TCGA T^CGA
XmaCI 423 CCCGGG C^CCGGG
XmaI 423 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AluI 413 AGCT AG^CT
518 AGCT
Asp700I 132 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
359 GAAATGATTC
AvaII 466 GGACC G^G[AT]CC
491 GGACC
BcnI 423 CCCGG CC^[GC]GG
424 CCGGG
BmyI 41 GTGCTC G[AGT]GC[ACT]^C
417 GTGCCC
BsmAI 8 GAGAC GTCTC(1/5)
27 GAGAC
Bsp1286I 41 GTGCTC G[AGT]GC[ACT]^C
417 GTGCCC
BstYI 59 AGATCC [GA]^GATC[CT]
460 AGATCT
Cac8I 305 GCAGGC GC[ACGT]^[ACGT]GC
345 GCCAGC
DsaI 32 CCACGG C^C[GA][CT]GG
267 CCGTGG
MflI 59 AGATCC [GA]^GATC[CT]
460 AGATCT
NciI 423 CCCGG CC^[GC]GG
424 CCGGG
XhoII 59 AGATCC [GA]^GATC[CT]
460 AGATCT
XmnI 132 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
359 GAAATGATTC
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# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
BcgI BsaJI BsiYI BslI BssKI
BstNI Cfr13I DdeI DpnII EarI
EcoRII HaeIII HinfI Ksp632I MboI
MboII MnlI MvaI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI TfiI