(Circular) (Six-base) MAPSORT of: pFN21AB8333.seq Check: 2837 from: 1 to: 5644 pFN21AB8333 5644 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3454 278 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2987 1937 Size: 1050 4594 Acc65I G'GTAC_C Cuts at: 2419 2965 2419 Size: 546 5098 AclI AA'CG_TT Cuts at: 1563 3771 4144 1563 Size: 2208 373 3063 Fragments arranged by size: 3063 2208 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2627 3707 5091 5359 1632 Size: 995 1080 1384 268 1917 Fragments arranged by size: 1917 1384 1080 995 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5447 AflIII A'CryG_T Cuts at: 1203 2854 2856 4543 5520 1203 Size: 1651 2 1687 977 1327 Fragments arranged by size: 1687 1651 1327 977 2 AgeI A'CCGG_T Cuts at: 4475 4475 Size: 5644 AhdI GACnn_n'nnGTC Cuts at: 2830 4373 2830 Size: 1543 4101 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5644 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2093 1184 Size: 909 4735 AlwNI CAG_nnn'CTG Cuts at: 3387 4959 3387 Size: 1572 4072 ApaLI G'TGCA_C Cuts at: 3701 4857 3701 Size: 1156 4488 ApoI r'AATT_y Cuts at: 1493 2953 3179 3331 5440 1493 Size: 1460 226 152 2109 1697 Fragments arranged by size: 2109 1697 1460 226 152 AseI AT'TA_AT Cuts at: 160 1032 4198 160 Size: 872 3166 1606 Fragments arranged by size: 3166 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 AvaI C'yCGr_G Cuts at: 1870 1943 2165 2969 1870 Size: 73 222 804 4545 Fragments arranged by size: 4545 804 222 73 BamHI G'GATC_C Cuts at: 2641 2974 3341 2641 Size: 333 367 4944 Fragments arranged by size: 4944 367 333 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2045 2096 2419 2550 2965 Size: 325 217 627 258 51 323 131 415 Cuts at: 2965 4420 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 415 325 323 258 217 131 51 BanII G_rGCy'C Cuts at: 1391 2439 2843 2963 1391 Size: 1048 404 120 4072 Fragments arranged by size: 4072 1048 404 120 BbeI G_GCGC'C Cuts at: 2100 2554 2100 Size: 454 5190 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4673 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2222 2256 3835 3869 1903 Size: 34 285 34 1579 34 3678 Fragments arranged by size: 3678 1579 285 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3536 4746 3536 Size: 1210 4434 BclI T'GATC_A Cuts at: 1548 1548 Size: 5644 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4255 136 Size: 107 122 71 631 605 2583 1525 Fragments arranged by size: 2583 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5639 5639 Size: 5644 BlpI GC'TnA_GC Cuts at: 3061 3061 Size: 5644 Bme1580I G_kGCm'C Cuts at: 1790 2410 2539 2910 3705 4861 1790 Size: 620 129 371 795 1156 2573 Fragments arranged by size: 2573 1156 795 620 371 129 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5644 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3375 4333 442 Size: 416 53 83 399 24 1958 958 1753 Fragments arranged by size: 1958 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5644 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2035 4304 1239 Size: 796 2269 2579 Fragments arranged by size: 2579 2269 796 Bpu10I CC'TnA_GC Cuts at: 2148 2741 2148 Size: 593 5051 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3769 4634 4932 3769 Size: 865 298 4481 Fragments arranged by size: 4481 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4307 882 Size: 617 2808 2219 Fragments arranged by size: 2808 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2857 3435 493 Size: 711 1653 578 2702 Fragments arranged by size: 2702 1653 711 578 BsaBI GATnn'nnATC Cuts at: 5638 5638 Size: 5644 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2097 2257 2551 3451 Size: 53 83 186 936 564 160 294 900 Cuts at: 3451 3833 4468 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 900 635 564 382 294 186 160 83 53 BsaWI w'CCGG_w Cuts at: 4077 4475 4749 4896 5307 4077 Size: 398 274 147 411 4414 Fragments arranged by size: 4414 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2876 2906 1178 Size: 30 165 30 1473 30 3916 Fragments arranged by size: 3916 1473 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2443 2642 1186 Size: 1257 199 4188 Fragments arranged by size: 4188 1257 199 BseYI C'CCAG_C Cuts at: 4847 5343 4847 Size: 496 5148 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2612 1163 Size: 573 876 4195 Fragments arranged by size: 4195 876 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2812 3094 3855 4883 1540 Size: 275 186 811 282 761 1028 2301 Fragments arranged by size: 2301 1028 811 761 282 275 186 BsiHKAI G_wGCw'C Cuts at: 2843 3705 3790 4861 2843 Size: 862 85 1071 3626 Fragments arranged by size: 3626 1071 862 85 BsmI GAATG_Cn' Cuts at: 2324 3155 3248 2324 Size: 831 93 4720 Fragments arranged by size: 4720 831 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2410 2439 2539 2843 2910 2963 3705 Size: 399 620 29 100 304 67 53 742 Cuts at: 3705 3790 4861 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 620 399 304 100 85 67 53 29 BspEI T'CCGG_A Cuts at: 5307 5307 Size: 5644 BspHI T'CATG_A Cuts at: 3533 5263 3533 Size: 1730 3914 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2895 2999 844 Size: 2051 104 3489 Fragments arranged by size: 3489 2051 104 BsrBI CCG'CTC Cuts at: 1394 1823 3531 1394 Size: 429 1708 3507 Fragments arranged by size: 3507 1708 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2070 4139 4313 65 Size: 1183 822 2069 174 1396 Fragments arranged by size: 2069 1396 1183 822 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4288 4475 1700 Size: 110 2478 187 2869 Fragments arranged by size: 2869 2478 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5644 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5644 BssSI C'ACGA_G Cuts at: 2540 3704 4716 2540 Size: 1164 1012 3468 Fragments arranged by size: 3468 1164 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5644 BstBI TT'CG_AA Cuts at: 4448 4448 Size: 5644 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5644 BstYI r'GATC_y Cuts at: 1972 2641 2728 2974 3341 3726 3743 5195 5281 Size: 669 87 246 367 385 17 1452 86 Cuts at: 5281 5293 5639 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 669 385 367 346 246 87 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2065 2554 513 Size: 552 939 61 489 3603 Fragments arranged by size: 3603 939 552 489 61 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4905 Fragments arranged by size: 4905 683 56 BtsI GCAGTG_nn' Cuts at: 3160 3954 3974 3160 Size: 794 20 4830 Fragments arranged by size: 4830 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5644 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2372 2407 529 Size: 35 1808 35 3766 Fragments arranged by size: 3766 1808 35 35 DraI TTT'AAA Cuts at: 2948 2948 Size: 5644 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4651 809 Size: 507 3335 1802 Fragments arranged by size: 3335 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2023 2062 2116 Size: 54 1425 50 275 18 193 39 54 Cuts at: 2116 2428 2579 2809 3091 3980 5609 8 Size: 312 151 230 282 889 1629 43 Fragments arranged by size: 1629 1425 889 312 282 275 230 193 151 54 54 50 43 39 18 EagI C'GGCC_G Cuts at: 1537 1812 2809 3091 1537 Size: 275 997 282 4090 Fragments arranged by size: 4090 997 282 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3574 1353 Size: 2221 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4231 4605 4751 387 Size: 1064 138 2642 374 146 1280 Fragments arranged by size: 2642 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2841 2841 Size: 5644 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2035 2627 3707 4304 5091 5359 1239 Size: 393 403 592 1080 597 787 268 1524 Fragments arranged by size: 1524 1080 787 597 592 403 393 268 EcoO109I rG'GnC_Cy Cuts at: 2632 2632 Size: 5644 EcoRI G'AATT_C Cuts at: 2953 3331 2953 Size: 378 5266 EcoRV GAT'ATC Cuts at: 2359 2359 Size: 5644 FspI TGC'GCA Cuts at: 4150 4150 Size: 5644 HaeII r_GCGC'y Cuts at: 1678 2100 2554 4791 1678 Size: 422 454 2237 2531 Fragments arranged by size: 2531 2237 454 422 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2144 2176 2816 2848 4285 Size: 32 135 32 572 32 640 32 1437 Cuts at: 4285 4317 4359 4391 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1437 640 572 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2275 2988 3234 669 Size: 988 281 337 713 246 3079 Fragments arranged by size: 3079 988 713 337 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5644 HpaI GTT'AAC Cuts at: 3234 3234 Size: 5644 KasI G'GCGC_C Cuts at: 2096 2550 2096 Size: 454 5190 KpnI G_GTAC'C Cuts at: 2423 2969 2423 Size: 546 5098 MfeI C'AATT_G Cuts at: 3243 3243 Size: 5644 MluI A'CGCG_T Cuts at: 5520 5520 Size: 5644 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2350 4758 4942 1305 Size: 1045 2408 184 2007 Fragments arranged by size: 2408 2007 1045 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2025 2430 10 Size: 54 1425 343 193 405 3224 Fragments arranged by size: 3224 1425 405 343 193 54 MslI CAynn'nnrTG Cuts at: 518 1166 3602 3961 4120 518 Size: 648 2436 359 159 2042 Fragments arranged by size: 2436 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2426 2565 3060 3737 4885 5130 1606 Size: 820 139 495 677 1148 245 2120 Fragments arranged by size: 2120 1148 820 677 495 245 139 NarI GG'CG_CC Cuts at: 2097 2551 2097 Size: 454 5190 NcoI C'CATG_G Cuts at: 513 1065 2004 2554 513 Size: 552 939 550 3603 Fragments arranged by size: 3603 939 552 550 NdeI CA'TA_TG Cuts at: 387 2616 387 Size: 2229 3415 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5644 NotI GC'GGCC_GC Cuts at: 3091 3091 Size: 5644 NspI r_CATG'y Cuts at: 1132 1229 3002 4547 1132 Size: 97 1773 1545 2229 Fragments arranged by size: 2229 1773 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5644 PciI A'CATG_T Cuts at: 4543 4543 Size: 5644 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5644 PmeI GTTT'AAAC Cuts at: 2948 2948 Size: 5644 PmlI CAC'GTG Cuts at: 2857 2857 Size: 5644 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3713 3745 5226 5258 3713 Size: 32 1481 32 4099 Fragments arranged by size: 4099 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2632 2632 Size: 5644 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5644 PsiI TTA'TAA Cuts at: 3214 3214 Size: 5644 PstI C_TGCA'G Cuts at: 830 2472 2640 2996 830 Size: 1642 168 356 3478 Fragments arranged by size: 3478 1642 356 168 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5644 PvuII CAG'CTG Cuts at: 2426 2565 2426 Size: 139 5505 SacI G_AGCT'C Cuts at: 2843 2843 Size: 5644 SalI G'TCGA_C Cuts at: 1936 2986 1936 Size: 1050 4594 SbfI CC_TGCA'GG Cuts at: 2640 2996 2640 Size: 356 5288 ScaI AGT'ACT Cuts at: 3892 5604 3892 Size: 1712 3932 SfcI C'TryA_G Cuts at: 826 1045 2468 2636 2992 4127 4808 4999 826 Size: 219 1423 168 356 1135 681 191 1471 Fragments arranged by size: 1471 1423 1135 681 356 219 191 168 SfoI GGC'GCC Cuts at: 2098 2552 2098 Size: 454 5190 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5644 SmaI CCC'GGG Cuts at: 1872 2971 1872 Size: 1099 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3748 4649 4911 820 Size: 197 926 1805 901 262 1553 Fragments arranged by size: 1805 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3435 493 Size: 2942 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5644 SphI G_CATG'C Cuts at: 1132 3002 1132 Size: 1870 3774 SspI AAT'ATT Cuts at: 5 52 3568 5 Size: 47 3516 2081 Fragments arranged by size: 3516 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5644 StyI C'CwwG_G Cuts at: 513 1065 2004 2554 3083 513 Size: 552 939 550 529 3074 Fragments arranged by size: 3074 939 552 550 529 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3671 3688 3841 4026 4471 325 Size: 1255 2091 17 153 185 445 1498 Fragments arranged by size: 2091 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3890 5602 96 Size: 274 80 33 51 1443 1913 1712 138 Fragments arranged by size: 1913 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5604 5636 5604 Size: 32 5612 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5644 XbaI T'CTAG_A Cuts at: 2260 2980 2260 Size: 720 4924 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5644 XmaI C'CCGG_G Cuts at: 1870 2969 1870 Size: 1099 4545 XmnI GAAnn'nnTTC Cuts at: 2657 3773 2657 Size: 1116 4528 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3452 276 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI