Restriction map for
pFN21AB8332
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII AccII AclI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp700I AspEI
AspI AviII AvrII BalI BamHI
BbrPI BbvCI BcgI BclI BcnI
BfrI BlnI BlpI Bpu1102I BsaI
BsiEI BsiWI BsmBI BsmI BspDI
BspLU11I BsrBI BsrFI BssHII BssKI
BstAPI BstBI BstNI BstUI Bsu36I
CelII Cfr10I ClaI CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRII EcoRV
EcoT22I FbaI FseI FspI HgaI
HpaI KspI MfeI MluI MluNI
MscI MunI MvaI MvnI NaeI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PstI PvuI RsrII SacII
SapI SbfI ScaI ScrFI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspI StuI SwaI
Tth111I XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 72 enzymes) #
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Acc65I 1599 GGTACC G^GTACC
AccIII 795 TCCGGA T^CCGGA
AcyI 301 GGCGCC G[GA]^CG[CT]C
AlwI 310 GGATC GGATC(4/5)
AseI 1783 ATTAAT AT^TAAT
AsnI 1783 ATTAAT AT^TAAT
Asp718I 1599 GGTACC G^GTACC
AvaI 371 CTCGAG C^[CT]CG[GA]G
BanII 626 GAGCTC G[GA]GC[CT]^C
BbeI 301 GGCGCC GGCGC^C
BbsI 679 GAAGAC GAAGAC(2/6)
BciVI 792 GTATCC GTATCC(6/5)
BfaI 314 CTAG C^TAG
BglI 1301 GCCGCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 153 AGATCT A^GATCT
BpuAI 679 GAAGAC GAAGAC(2/6)
BsaAI 384 CACGTA [CT]AC^GT[GA]
BsaHI 301 GGCGCC G[GA]^CG[CT]C
BsaWI 795 TCCGGA [AT]^CCGG[AT]
BseAI 795 TCCGGA T^CCGGA
BsgI 1055 CTGCAC GTGCAG(16/14)
BsmAI 240 GTCTC GTCTC(1/5)
BsmFI 226 GTCCC GGGAC(10/14)
BsoBI 371 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1289 TGTACA T^GTACA
BspEI 795 TCCGGA T^CCGGA
BsrGI 1289 TGTACA T^GTACA
BssSI 805 CTCGTG CACGAG(-5/-1)
Bst1107I 387 GTATAC GTA^TAC
BstEII 1517 GGTCACC G^GT[ACGT]ACC
BstPI 1517 GGTCACC G^GT[ACGT]ACC
BstXI 285 CCAAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 387 GTATAC GTA^TAC
CfoI 302 GCGC GCG^C
DraI 498 TTTAAA TTT^AAA
DraII 321 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 1224 GACATCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1764 TGGCCG [CT]^GGCC[GA]
EcoO109I 321 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1517 GGTCACC G^GT[ACGT]ACC
EcoT14I 1305 CCATGG C^C[AT][AT]GG
FauI 1739 CCCGC CCCGC(4/6)
HaeII 301 GGCGCC [GA]GCGC^[CT]
HapII 796 CCGG C^CGG
HhaI 302 GCGC GCG^C
Hin1I 301 GGCGCC G[GA]^CG[CT]C
HinP1I 302 GCGC G^CGC
HindIII 1441 AAGCTT A^AGCTT
HpaII 796 CCGG C^CGG
KasI 301 GGCGCC G^GCGCC
KpnI 1599 GGTACC GGTAC^C
MaeI 314 CTAG C^TAG
MroI 795 TCCGGA T^CCGGA
MspI 796 CCGG C^CGG
NarI 301 GGCGCC GG^CGCC
NcoI 1305 CCATGG C^CATGG
PaeR7I 371 CTCGAG C^TCGAG
PflMI 1354 CCAATCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 321 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 1783 ATTAAT AT^TAAT
SacI 626 GAGCTC GAGCT^C
SalI 358 GTCGAC G^TCGAC
SfcI 660 CTACAG C^T[GA][CT]AG
SfoI 301 GGCGCC GGC^GCC
SmlI 371 CTCGAG C^T[CT][GA]AG
SphI 1297 GCATGC GCATG^C
SspBI 1289 TGTACA T^GTACA
StyI 1305 CCATGG C^C[AT][AT]GG
Van91I 1354 CCAATCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 313 TCTAGA T^CTAGA
XcmI 1610 CCAGCATTTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 371 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AciI 1302 CCGC CCGC(-3/-1)
1740 CCGC
AspHI 261 GAGCAC G[AT]GC[AT]^C
626 GAGCTC
BanI 301 GGCGCC G^G[CT][GA]CC
1599 GGTACC
BsaBI 642 GATTAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1116 GATTAGCATC
BsaJI 305 CCGTGG C^C[ACGT][ACGT]GG
1305 CCATGG
BsiHKAI 261 GAGCAC G[AT]GC[AT]^C
626 GAGCTC
BspHI 537 TCATGA T^CATGA
840 TCATGA
BspMI 1830 ACCTGC ACCTGC(4/8)
703 GCAGGT
BsrDI 185 CATTGC GCAATG(2/0)
1742 GCAATG
BstYI 153 AGATCT [GA]^GATC[CT]
310 GGATCT
Cac8I 1297 GCATGC GC[ACGT]^[ACGT]GC
1609 GCCAGC
DpnII 154 GATC ^GATC
311 GATC
DsaI 305 CCGTGG C^C[GA][CT]GG
1305 CCATGG
HincII 358 GTCGAC GT[CT]^[GA]AC
1252 GTCAAC
HindII 358 GTCGAC GT[CT]^[GA]AC
1252 GTCAAC
HphI 1684 GGTGA GGTGA(8/7)
1519 TCACC
MaeII 36 ACGT A^CGT
385 ACGT
MamI 642 GATTAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1116 GATTAGCATC
MboI 154 GATC ^GATC
311 GATC
MflI 153 AGATCT [GA]^GATC[CT]
310 GGATCT
MslI 1369 CATTGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1614 CATTTCTGTG
NdeII 154 GATC ^GATC
311 GATC
NspI 1160 ACATGC [GA]CATG^[CT]
1297 GCATGC
RcaI 537 TCATGA T^CATGA
840 TCATGA
Sau3AI 154 GATC ^GATC
311 GATC
XhoII 153 AGATCT [GA]^GATC[CT]
310 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AccI AcsI AfaI AluI AlwNI
ApoI AvaII BbvI BmyI BpmI
BseRI BsiYI BslI Bsp1286I BsrI
BstF5I Cfr13I DdeI EarI Fnu4HI
FokI HaeIII HinfI ItaI Ksp632I
MaeIII MboII MnlI MseI MspA1I
MwoI NlaIII NlaIV PleI PvuII
RsaI Sau96I SfaNI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I