(Circular) (Six-base) MAPSORT of: pFN21AB8332.seq Check: 196 from: 1 to: 6541 pFN21AB8332 6541 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2699 2699 Size: 6541 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4351 278 Size: 53 83 186 936 2815 2468 Fragments arranged by size: 2815 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2364 2393 3399 3884 1937 Size: 427 29 1006 485 4594 Fragments arranged by size: 4594 1006 485 427 29 Acc65I G'GTAC_C Cuts at: 3604 3862 3604 Size: 258 6283 AclI AA'CG_TT Cuts at: 1563 4668 5041 1563 Size: 3105 373 3063 Fragments arranged by size: 3105 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2205 2508 2574 3717 4604 5988 6256 1632 Size: 573 303 66 1143 887 1384 268 1917 Fragments arranged by size: 1917 1384 1143 887 573 303 268 66 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6344 AflIII A'CryG_T Cuts at: 1203 5440 6417 1203 Size: 4237 977 1327 Fragments arranged by size: 4237 1327 977 AgeI A'CCGG_T Cuts at: 5372 5372 Size: 6541 AhdI GACnn_n'nnGTC Cuts at: 5270 5270 Size: 6541 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6541 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6541 AlwNI CAG_nnn'CTG Cuts at: 2056 3059 3224 4284 5856 2056 Size: 1003 165 1060 1572 2741 Fragments arranged by size: 2741 1572 1060 1003 165 ApaLI G'TGCA_C Cuts at: 4598 5754 4598 Size: 1156 5385 ApoI r'AATT_y Cuts at: 1493 2588 2951 3144 3850 4076 4228 6337 1493 Size: 1095 363 193 706 226 152 2109 1697 Fragments arranged by size: 2109 1697 1095 706 363 226 193 152 AseI AT'TA_AT Cuts at: 160 1032 3789 5095 160 Size: 872 2757 1306 1606 Fragments arranged by size: 2757 1606 1306 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6541 AvaI C'yCGr_G Cuts at: 1870 1943 2376 3866 1870 Size: 73 433 1490 4545 Fragments arranged by size: 4545 1490 433 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3588 3621 3588 Size: 33 6508 BamHI G'GATC_C Cuts at: 3871 4238 3871 Size: 367 6174 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2306 3604 3862 5317 618 Size: 325 217 627 519 1298 258 1455 1842 Fragments arranged by size: 1842 1455 1298 627 519 325 258 217 BanII G_rGCy'C Cuts at: 1391 2635 3860 1391 Size: 1244 1225 4072 Fragments arranged by size: 4072 1244 1225 BbeI G_GCGC'C Cuts at: 2310 2310 Size: 6541 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2691 928 Size: 971 792 4778 Fragments arranged by size: 4778 971 792 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4732 4766 1903 Size: 34 2795 34 3678 Fragments arranged by size: 3678 2795 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2808 4433 5643 2808 Size: 1625 1210 3706 Fragments arranged by size: 3706 1625 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6541 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3312 5152 136 Size: 107 122 71 631 605 1640 1840 1525 Fragments arranged by size: 1840 1640 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2158 6536 2158 Size: 4378 2163 BlpI GC'TnA_GC Cuts at: 3958 3958 Size: 6541 Bme1580I G_kGCm'C Cuts at: 1790 3763 4602 5758 1790 Size: 1973 839 1156 2573 Fragments arranged by size: 2573 1973 1156 839 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6541 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4272 5230 442 Size: 416 53 83 399 24 2855 958 1753 Fragments arranged by size: 2855 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6541 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2199 2319 2397 3015 5201 1239 Size: 960 120 78 618 2186 2579 Fragments arranged by size: 2579 2186 960 618 120 78 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4666 5531 5829 4666 Size: 865 298 5378 Fragments arranged by size: 5378 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5204 882 Size: 617 3705 2219 Fragments arranged by size: 3705 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2391 4332 493 Size: 711 1187 1941 2702 Fragments arranged by size: 2702 1941 1187 711 BsaBI GATnn'nnATC Cuts at: 2651 3125 6535 2651 Size: 474 3410 2657 Fragments arranged by size: 3410 2657 474 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2307 4348 4730 5365 Size: 53 83 186 936 774 2041 382 635 Cuts at: 5365 275 Size: 1451 Fragments arranged by size: 2041 1451 936 774 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2800 4974 5372 5646 5793 6204 2800 Size: 2174 398 274 147 411 3137 Fragments arranged by size: 3137 2174 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2395 2425 1178 Size: 30 165 30 992 30 5294 Fragments arranged by size: 5294 992 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2226 2364 3827 1186 Size: 1040 138 1463 3900 Fragments arranged by size: 3900 1463 1040 138 BseYI C'CCAG_C Cuts at: 3535 5744 6240 3535 Size: 2209 496 3836 Fragments arranged by size: 3836 2209 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3045 1163 Size: 573 1309 4659 Fragments arranged by size: 4659 1309 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3991 4752 5780 1540 Size: 275 186 1990 761 1028 2301 Fragments arranged by size: 2301 1990 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2270 2635 4602 4687 5758 2270 Size: 365 1967 85 1071 3053 Fragments arranged by size: 3053 1967 1071 365 85 BsmI GAATG_Cn' Cuts at: 4052 4145 4052 Size: 93 6448 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2270 2635 3763 3860 4602 4687 5758 Size: 399 480 365 1128 97 742 85 1071 Cuts at: 5758 1391 Size: 2174 Fragments arranged by size: 2174 1128 1071 742 480 399 365 97 85 BspEI T'CCGG_A Cuts at: 2800 6204 2800 Size: 3404 3137 BspHI T'CATG_A Cuts at: 2542 2845 4430 6160 2542 Size: 303 1585 1730 2923 Fragments arranged by size: 2923 1730 1585 303 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2699 3844 3896 844 Size: 1855 1145 52 3489 Fragments arranged by size: 3489 1855 1145 52 BsrBI CCG'CTC Cuts at: 1394 1823 4428 1394 Size: 429 2605 3507 Fragments arranged by size: 3507 2605 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2189 3754 5036 5210 65 Size: 1183 941 1565 1282 174 1396 Fragments arranged by size: 1565 1396 1282 1183 941 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5185 5372 1700 Size: 110 3375 187 2869 Fragments arranged by size: 3375 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3294 96 Size: 3198 3343 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6541 BssSI C'ACGA_G Cuts at: 2810 4601 5613 2810 Size: 1791 1012 3738 Fragments arranged by size: 3738 1791 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6541 BstBI TT'CG_AA Cuts at: 5345 5345 Size: 6541 BstEII G'GTnAC_C Cuts at: 1184 3522 1184 Size: 2338 4203 BstXI CCAn_nnnn'nTGG Cuts at: 2297 2297 Size: 6541 BstYI r'GATC_y Cuts at: 1972 2158 2315 3871 4238 4623 4640 6092 6178 Size: 186 157 1556 367 385 17 1452 86 Cuts at: 6178 6190 6536 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1556 1452 385 367 346 186 157 86 17 12 BstZ17I GTA'TAC Cuts at: 2394 2394 Size: 6541 BtgI C'CryG_G Cuts at: 513 1065 2310 3310 513 Size: 552 1245 1000 3744 Fragments arranged by size: 3744 1245 1000 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5802 Fragments arranged by size: 5802 683 56 BtsI GCAGTG_nn' Cuts at: 2914 4057 4851 4871 2914 Size: 1143 794 20 4584 Fragments arranged by size: 4584 1143 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6541 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6506 DraI TTT'AAA Cuts at: 2505 3845 2505 Size: 1340 5201 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3235 5548 809 Size: 507 1919 2313 1802 Fragments arranged by size: 2313 1919 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3769 3988 4877 Size: 54 1425 50 275 18 1939 219 889 Cuts at: 4877 6506 8 Size: 1629 43 Fragments arranged by size: 1939 1629 1425 889 275 219 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3988 1537 Size: 275 2176 4090 Fragments arranged by size: 4090 2176 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2075 2436 2487 4471 1353 Size: 722 361 51 1984 3423 Fragments arranged by size: 3423 1984 722 361 51 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5128 5502 5648 387 Size: 1064 138 3539 374 146 1280 Fragments arranged by size: 3539 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2633 2633 Size: 6541 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2199 2205 2319 2397 2508 2574 3015 Size: 393 567 6 114 78 111 66 441 Cuts at: 3015 3717 4604 5201 5988 6256 1239 Size: 702 887 597 787 268 1524 Fragments arranged by size: 1524 887 787 702 597 567 441 393 268 114 111 78 66 6 EcoO109I rG'GnC_Cy Cuts at: 2327 2327 Size: 6541 EcoRI G'AATT_C Cuts at: 3850 4228 3850 Size: 378 6163 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2210 2210 Size: 6541 FspI TGC'GCA Cuts at: 5047 5047 Size: 6541 HaeII r_GCGC'y Cuts at: 1678 2310 5688 1678 Size: 632 3378 2531 Fragments arranged by size: 3378 2531 632 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2370 2402 2738 2770 2774 Size: 32 135 32 798 32 336 32 4 Cuts at: 2774 2806 5182 5214 5256 5288 1373 Size: 32 2376 32 42 32 2626 Fragments arranged by size: 2626 2376 798 336 135 42 32 32 32 32 32 32 32 4 HincII GTy'rAC Cuts at: 669 1657 1938 2365 3259 3885 4131 669 Size: 988 281 427 894 626 246 3079 Fragments arranged by size: 3079 988 894 626 427 281 246 HindIII A'AGCT_T Cuts at: 748 3446 748 Size: 2698 3843 HpaI GTT'AAC Cuts at: 4131 4131 Size: 6541 KasI G'GCGC_C Cuts at: 2306 2306 Size: 6541 KpnI G_GTAC'C Cuts at: 3608 3866 3608 Size: 258 6283 MfeI C'AATT_G Cuts at: 4140 4140 Size: 6541 MluI A'CGCG_T Cuts at: 6417 6417 Size: 6541 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2016 3076 3229 3370 5655 5839 1305 Size: 711 1060 153 141 2285 184 2007 Fragments arranged by size: 2285 2007 1060 711 184 153 141 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4719 Fragments arranged by size: 4719 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3378 3623 4499 4858 5017 518 Size: 648 2212 245 876 359 159 2042 Fragments arranged by size: 2212 2042 876 648 359 245 159 MspA1I CmG'CkG Cuts at: 1606 2927 3059 3386 3957 4634 5782 6027 1606 Size: 1321 132 327 571 677 1148 245 2120 Fragments arranged by size: 2120 1321 1148 677 571 327 245 132 NarI GG'CG_CC Cuts at: 2307 2307 Size: 6541 NcoI C'CATG_G Cuts at: 513 1065 3310 513 Size: 552 2245 3744 Fragments arranged by size: 3744 2245 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6541 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6541 NotI GC'GGCC_GC Cuts at: 3988 3988 Size: 6541 NspI r_CATG'y Cuts at: 1132 1229 3169 3306 3899 5444 1132 Size: 97 1940 137 593 1545 2229 Fragments arranged by size: 2229 1940 1545 593 137 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6541 PciI A'CATG_T Cuts at: 5440 5440 Size: 6541 PflMI CCAn_nnn'nTGG Cuts at: 1108 3365 1108 Size: 2257 4284 PmeI GTTT'AAAC Cuts at: 3845 3845 Size: 6541 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4610 4642 6123 6155 4610 Size: 32 1481 32 4996 Fragments arranged by size: 4996 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2327 2327 Size: 6541 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6541 PsiI TTA'TAA Cuts at: 4111 4111 Size: 6541 PstI C_TGCA'G Cuts at: 830 3893 830 Size: 3063 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6541 PvuII CAG'CTG Cuts at: 2927 3059 3386 2927 Size: 132 327 6082 Fragments arranged by size: 6082 327 132 SacI G_AGCT'C Cuts at: 2635 2635 Size: 6541 SalI G'TCGA_C Cuts at: 1936 2363 3883 1936 Size: 427 1520 4594 Fragments arranged by size: 4594 1520 427 SbfI CC_TGCA'GG Cuts at: 3893 3893 Size: 6541 ScaI AGT'ACT Cuts at: 4789 6501 4789 Size: 1712 4829 SfcI C'TryA_G Cuts at: 826 1045 2665 3889 5024 5705 5896 826 Size: 219 1620 1224 1135 681 191 1471 Fragments arranged by size: 1620 1471 1224 1135 681 219 191 SfoI GGC'GCC Cuts at: 2308 2308 Size: 6541 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6541 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6541 SmaI CCC'GGG Cuts at: 1872 3868 1872 Size: 1996 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2376 4645 5546 5808 820 Size: 197 926 433 2269 901 262 1553 Fragments arranged by size: 2269 1553 926 901 433 262 197 SnaBI TAC'GTA Cuts at: 493 4332 493 Size: 3839 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6541 SphI G_CATG'C Cuts at: 1132 3306 3899 1132 Size: 2174 593 3774 Fragments arranged by size: 3774 2174 593 SspI AAT'ATT Cuts at: 5 52 4465 5 Size: 47 4413 2081 Fragments arranged by size: 4413 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6541 StyI C'CwwG_G Cuts at: 513 1065 3310 3980 513 Size: 552 2245 670 3074 Fragments arranged by size: 3074 2245 670 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3549 3677 4568 4585 4738 4923 5368 Size: 1255 1969 128 891 17 153 185 445 Cuts at: 5368 325 Size: 1498 Fragments arranged by size: 1969 1498 1255 891 445 185 153 128 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3294 4787 6499 Size: 274 80 33 51 1443 1317 1493 1712 Cuts at: 6499 96 Size: 138 Fragments arranged by size: 1712 1493 1443 1317 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6501 6533 6501 Size: 32 6509 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6541 XbaI T'CTAG_A Cuts at: 2318 3877 2318 Size: 1559 4982 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3622 3622 Size: 6541 XhoI C'TCGA_G Cuts at: 1943 2376 1943 Size: 433 6108 XmaI C'CCGG_G Cuts at: 1870 3866 1870 Size: 1996 4545 XmnI GAAnn'nnTTC Cuts at: 4670 4670 Size: 6541 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4349 276 Size: 53 83 186 936 2815 2468 Fragments arranged by size: 2815 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI