Restriction map for
pFN21AB8332
(Linear) (Six-base) MAPSORT of: KIBB8332.seq Check: 3381 from: 1 to: 1836
KIBB8332.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 694 1836
Size: 694 1142
AccI GT'mk_AC
Cuts at: 0 359 388 1394 1836
Size: 359 29 1006 442
Fragments arranged by size:
1006 442 359 29
Acc65I G'GTAC_C
Cuts at: 0 1599 1836
Size: 1599 237
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 503 569 1712 1836
Size: 200 303 66 1143 124
Fragments arranged by size:
1143 303 200 124 66
AlwNI CAG_nnn'CTG
Cuts at: 0 51 1054 1219 1836
Size: 51 1003 165 617
Fragments arranged by size:
1003 617 165 51
ApoI r'AATT_y
Cuts at: 0 583 946 1139 1836
Size: 583 363 193 697
Fragments arranged by size:
697 583 363 193
AseI AT'TA_AT
Cuts at: 0 1784 1836
Size: 1784 52
AvaI C'yCGr_G
Cuts at: 0 371 1836
Size: 371 1465
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1583 1616 1836
Size: 1583 33 220
Fragments arranged by size:
1583 220 33
BanI G'GyrC_C
Cuts at: 0 301 1599 1836
Size: 301 1298 237
Fragments arranged by size:
1298 301 237
BanII G_rGCy'C
Cuts at: 0 630 1836
Size: 630 1206
BbeI G_GCGC'C
Cuts at: 0 305 1836
Size: 305 1531
BbsI GAAGACnn'nnnn_
Cuts at: 0 686 1836
Size: 686 1150
BciVI GTATCCnnnnn_n'
Cuts at: 0 803 1836
Size: 803 1033
BglI GCCn_nnn'nGGC
Cuts at: 0 1307 1836
Size: 1307 529
BglII A'GATC_T
Cuts at: 0 153 1836
Size: 153 1683
Bme1580I G_kGCm'C
Cuts at: 0 1758 1836
Size: 1758 78
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 314 392 1010 1836
Size: 194 120 78 618 826
Fragments arranged by size:
826 618 194 120 78
BsaAI yAC'GTr
Cuts at: 0 386 1836
Size: 386 1450
BsaBI GATnn'nnATC
Cuts at: 0 646 1120 1836
Size: 646 474 716
Fragments arranged by size:
716 646 474
BsaHI Gr'CG_yC
Cuts at: 0 302 1836
Size: 302 1534
BsaWI w'CCGG_w
Cuts at: 0 795 1836
Size: 795 1041
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 390 420 1836
Size: 390 30 1416
Fragments arranged by size:
1416 390 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 221 359 1822 1836
Size: 221 138 1463 14
Fragments arranged by size:
1463 221 138 14
BseYI C'CCAG_C
Cuts at: 0 1530 1836
Size: 1530 306
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1040 1836
Size: 1040 796
BsiHKAI G_wGCw'C
Cuts at: 0 265 630 1836
Size: 265 365 1206
Fragments arranged by size:
1206 365 265
Bsp1286I G_dGCh'C
Cuts at: 0 265 630 1758 1836
Size: 265 365 1128 78
Fragments arranged by size:
1128 365 265 78
BspEI T'CCGG_A
Cuts at: 0 795 1836
Size: 795 1041
BspHI T'CATG_A
Cuts at: 0 537 840 1836
Size: 537 303 996
Fragments arranged by size:
996 537 303
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 694 1836
Size: 694 1142
BsrDI GCAATG_nn'
Cuts at: 0 184 1749 1836
Size: 184 1565 87
Fragments arranged by size:
1565 184 87
BsrGI T'GTAC_A
Cuts at: 0 1289 1836
Size: 1289 547
BssSI C'ACGA_G
Cuts at: 0 805 1836
Size: 805 1031
BstEII G'GTnAC_C
Cuts at: 0 1517 1836
Size: 1517 319
BstXI CCAn_nnnn'nTGG
Cuts at: 0 292 1836
Size: 292 1544
BstYI r'GATC_y
Cuts at: 0 153 310 1836
Size: 153 157 1526
Fragments arranged by size:
1526 157 153
BstZ17I GTA'TAC
Cuts at: 0 389 1836
Size: 389 1447
BtgI C'CryG_G
Cuts at: 0 305 1305 1836
Size: 305 1000 531
Fragments arranged by size:
1000 531 305
BtsI GCAGTG_nn'
Cuts at: 0 909 1836
Size: 909 927
DraI TTT'AAA
Cuts at: 0 500 1836
Size: 500 1336
DrdI GACnn_nn'nnGTC
Cuts at: 0 1230 1836
Size: 1230 606
EaeI y'GGCC_r
Cuts at: 0 1764 1836
Size: 1764 72
EarI CTCTTCn'nnn_
Cuts at: 0 70 431 482 1836
Size: 70 361 51 1354
Fragments arranged by size:
1354 361 70 51
EcoICRI GAG'CTC
Cuts at: 0 628 1836
Size: 628 1208
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 200 314 392 503 569 1010 1712
Size: 194 6 114 78 111 66 441 702
Cuts at: 1712 1836
Size: 124
Fragments arranged by size:
702 441 194 124 114 111 78 66
6
EcoO109I rG'GnC_Cy
Cuts at: 0 322 1836
Size: 322 1514
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 205 1836
Size: 205 1631
HaeII r_GCGC'y
Cuts at: 0 305 1836
Size: 305 1531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 365 397 733 765 769 801 1836
Size: 365 32 336 32 4 32 1035
Fragments arranged by size:
1035 365 336 32 32 32 4
HincII GTy'rAC
Cuts at: 0 360 1254 1836
Size: 360 894 582
Fragments arranged by size:
894 582 360
HindIII A'AGCT_T
Cuts at: 0 1441 1836
Size: 1441 395
KasI G'GCGC_C
Cuts at: 0 301 1836
Size: 301 1535
KpnI G_GTAC'C
Cuts at: 0 1603 1836
Size: 1603 233
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 11 1071 1224 1365 1836
Size: 11 1060 153 141 471
Fragments arranged by size:
1060 471 153 141 11
MslI CAynn'nnrTG
Cuts at: 0 1373 1618 1836
Size: 1373 245 218
Fragments arranged by size:
1373 245 218
MspA1I CmG'CkG
Cuts at: 0 922 1054 1381 1836
Size: 922 132 327 455
Fragments arranged by size:
922 455 327 132
NarI GG'CG_CC
Cuts at: 0 302 1836
Size: 302 1534
NcoI C'CATG_G
Cuts at: 0 1305 1836
Size: 1305 531
NspI r_CATG'y
Cuts at: 0 1164 1301 1836
Size: 1164 137 535
Fragments arranged by size:
1164 535 137
PflMI CCAn_nnn'nTGG
Cuts at: 0 1360 1836
Size: 1360 476
PpuMI rG'GwC_Cy
Cuts at: 0 322 1836
Size: 322 1514
PvuII CAG'CTG
Cuts at: 0 922 1054 1381 1836
Size: 922 132 327 455
Fragments arranged by size:
922 455 327 132
SacI G_AGCT'C
Cuts at: 0 630 1836
Size: 630 1206
SalI G'TCGA_C
Cuts at: 0 358 1836
Size: 358 1478
SfcI C'TryA_G
Cuts at: 0 660 1836
Size: 660 1176
SfoI GGC'GCC
Cuts at: 0 303 1836
Size: 303 1533
SmlI C'TyrA_G
Cuts at: 0 371 1836
Size: 371 1465
SphI G_CATG'C
Cuts at: 0 1301 1836
Size: 1301 535
StyI C'CwwG_G
Cuts at: 0 1305 1836
Size: 1305 531
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1544 1672 1836
Size: 1544 128 164
Fragments arranged by size:
1544 164 128
TatI w'GTAC_w
Cuts at: 0 1289 1836
Size: 1289 547
XbaI T'CTAG_A
Cuts at: 0 313 1836
Size: 313 1523
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1617 1836
Size: 1617 219
XhoI C'TCGA_G
Cuts at: 0 371 1836
Size: 371 1465
Enzymes that do cut:
AarI AccI Acc65I AcuI AlwNI ApoI AseI AvaI
BaeI BanI BanII BbeI BbsI BciVI BglI BglII
Bme1580I BpmI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrDI
BsrGI BssSI BstEII BstXI BstYI BstZ17I BtgI BtsI
DraI DrdI EaeI EarI EcoICRI Eco57MI EcoO109I FalI
HaeII Hin4I HincII HindIII KasI KpnI MmeI MslI
MspA1I NarI NcoI NspI PflMI PpuMI PvuII SacI
SalI SfcI SfoI SmlI SphI StyI TaqII TatI
XbaI XcmI XhoI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AsiSI AvrII BamHI
BbvCI BcgI BclI BfrBI BlpI BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaI BsiEI BsiWI BsmI BsmBI
BsrBI BsrFI BssHII BstAPI BstBI Bsu36I BtgZI ClaI
CspCI DraIII EagI EciI EcoNI EcoRI EcoRV FseI
FspI FspAI HpaI MfeI MluI MscI NaeI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacII SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI StuI SwaI TstI Tth111I XmaI XmnI
ZraI