Restriction map for
pFN21AB8331
(Circular) (Six-base) MAPSORT of: pFN21AB8331.seq Check: 914 from: 1 to: 5335
pFN21AB8331 5335 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3145 278
Size: 53 83 186 936 1609 2468
Fragments arranged by size:
2468 1609 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2678 1937
Size: 741 4594
Acc65I G'GTAC_C
Cuts at: 2656 2656
Size: 5335
AclI AA'CG_TT
Cuts at: 1563 3462 3835 1563
Size: 1899 373 3063
Fragments arranged by size:
3063 1899 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2393 3398 4782 5050 1632
Size: 761 1005 1384 268 1917
Fragments arranged by size:
1917 1384 1005 761 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5138
AflIII A'CryG_T
Cuts at: 1203 4234 5211 1203
Size: 3031 977 1327
Fragments arranged by size:
3031 1327 977
AgeI A'CCGG_T
Cuts at: 4166 4166
Size: 5335
AhdI GACnn_n'nnGTC
Cuts at: 4064 4064
Size: 5335
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5335
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2220 1184
Size: 1036 4299
AlwNI CAG_nnn'CTG
Cuts at: 3078 4650 3078
Size: 1572 3763
ApaLI G'TGCA_C
Cuts at: 3392 4548 3392
Size: 1156 4179
ApoI r'AATT_y
Cuts at: 1493 2644 2870 3022 5131 1493
Size: 1151 226 152 2109 1697
Fragments arranged by size:
2109 1697 1151 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3889 160
Size: 872 2857 1606
Fragments arranged by size:
2857 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5335
AvaI C'yCGr_G
Cuts at: 1870 1943 2139 2164 2334 2567 2660 1870
Size: 73 196 25 170 233 93 4545
Fragments arranged by size:
4545 233 196 170 93 73 25
BamHI G'GATC_C
Cuts at: 2325 2471 2665 3032 2325
Size: 146 194 367 4628
Fragments arranged by size:
4628 367 194 146
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2117 2180 2204 2229 2656
Size: 325 217 627 330 63 24 25 427
Cuts at: 2656 4111 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 427 330 325 217 63
25 24
BanII G_rGCy'C
Cuts at: 1391 2578 2654 1391
Size: 1187 76 4072
Fragments arranged by size:
4072 1187 76
BbeI G_GCGC'C
Cuts at: 2184 2184
Size: 5335
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4364
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2145 2179 2581 2615 3526
Size: 34 50 34 124 34 402 34 911
Cuts at: 3526 3560 1903
Size: 34 3678
Fragments arranged by size:
3678 911 402 124 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3227 4437 3227
Size: 1210 4125
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5335
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2061 2088
Size: 107 122 71 631 605 337 52 27
Cuts at: 2088 3946 136
Size: 1858 1525
Fragments arranged by size:
1858 1525 631 605 337 122 107 71
52 27
BglII A'GATC_T
Cuts at: 2503 5330 2503
Size: 2827 2508
BlpI GC'TnA_GC
Cuts at: 2752 2752
Size: 5335
Bme1580I G_kGCm'C
Cuts at: 1790 2027 2234 3396 4552 1790
Size: 237 207 1162 1156 2573
Fragments arranged by size:
2573 1162 1156 237 207
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5335
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3066 4024 442
Size: 416 53 83 399 24 1649 958 1753
Fragments arranged by size:
1753 1649 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5335
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3995 1239
Size: 2756 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2288 3460 4325 4623 2288
Size: 1172 865 298 3000
Fragments arranged by size:
3000 1172 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2533 3998 882
Size: 617 1034 1465 2219
Fragments arranged by size:
2219 1465 1034 617
BsaAI yAC'GTr
Cuts at: 493 1204 3126 493
Size: 711 1922 2702
Fragments arranged by size:
2702 1922 711
BsaBI GATnn'nnATC
Cuts at: 5329 5329
Size: 5335
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2181 3142 3524 4159
Size: 53 83 186 936 648 961 382 635
Cuts at: 4159 275
Size: 1451
Fragments arranged by size:
1451 961 936 648 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3768 4166 4440 4587 4998 3768
Size: 398 274 147 411 4105
Fragments arranged by size:
4105 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5110
Fragments arranged by size:
5110 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2190 1186
Size: 1004 4331
BseYI C'CCAG_C
Cuts at: 2438 2467 4538 5034 2438
Size: 29 2071 496 2739
Fragments arranged by size:
2739 2071 496 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2611 1163
Size: 573 875 3887
Fragments arranged by size:
3887 875 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2785 3546 4574 1540
Size: 275 186 784 761 1028 2301
Fragments arranged by size:
2301 1028 784 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3396 3481 4552 3396
Size: 85 1071 4179
Fragments arranged by size:
4179 1071 85
BsmI GAATG_Cn'
Cuts at: 2070 2846 2939 2070
Size: 776 93 4466
Fragments arranged by size:
4466 776 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2027 2234 2578 2654 3396 3481 4552
Size: 399 237 207 344 76 742 85 1071
Cuts at: 4552 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 344 237 207 85
76
BspEI T'CCGG_A
Cuts at: 4998 4998
Size: 5335
BspHI T'CATG_A
Cuts at: 3224 4954 3224
Size: 1730 3605
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2690 844
Size: 1846 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2350 3222 1394
Size: 429 527 872 3507
Fragments arranged by size:
3507 872 527 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2098 3830 4004 65
Size: 1183 850 1732 174 1396
Fragments arranged by size:
1732 1396 1183 850 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3979 4166 1700
Size: 110 2169 187 2869
Fragments arranged by size:
2869 2169 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5335
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5335
BssSI C'ACGA_G
Cuts at: 3395 4407 3395
Size: 1012 4323
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5335
BstBI TT'CG_AA
Cuts at: 4139 4139
Size: 5335
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5335
BstXI CCAn_nnnn'nTGG
Cuts at: 2585 2585
Size: 5335
BstYI r'GATC_y
Cuts at: 1972 2325 2471 2503 2665 3032 3417 3434 4886
Size: 353 146 32 162 367 385 17 1452
Cuts at: 4886 4972 4984 5330 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 353 346 162 146
86 32 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2121 2186 2301 513
Size: 552 1056 65 115 3547
Fragments arranged by size:
3547 1056 552 115 65
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2058 487
Size: 683 56 832 3764
Fragments arranged by size:
3764 832 683 56
BtsI GCAGTG_nn'
Cuts at: 2851 3645 3665 2851
Size: 794 20 4521
Fragments arranged by size:
4521 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5335
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5300
DraI TTT'AAA
Cuts at: 2639 2639
Size: 5335
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4342 809
Size: 507 3026 1802
Fragments arranged by size:
3026 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2782 3671 5300
Size: 54 1425 50 275 18 952 889 1629
Cuts at: 5300 8
Size: 43
Fragments arranged by size:
1629 1425 952 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2782 1537
Size: 275 970 4090
Fragments arranged by size:
4090 970 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3265 1353
Size: 1912 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2458 2464 3922 4296 4442 387
Size: 1064 138 869 6 1458 374 146 1280
Fragments arranged by size:
1458 1280 1064 869 374 146 138 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2393 3398 3995 4782 5050 1239
Size: 393 761 1005 597 787 268 1524
Fragments arranged by size:
1524 1005 787 761 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2063 2135 2160 2063
Size: 72 25 5238
Fragments arranged by size:
5238 72 25
EcoRI G'AATT_C
Cuts at: 2644 3022 2644
Size: 378 4957
FspI TGC'GCA
Cuts at: 2217 3841 2217
Size: 1624 3711
HaeII r_GCGC'y
Cuts at: 1678 2184 2439 4482 1678
Size: 506 255 2043 2531
Fragments arranged by size:
2531 2043 506 255
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3976 4008 4050 4082 1373
Size: 32 135 32 2404 32 42 32 2626
Fragments arranged by size:
2626 2404 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2679 2925 669
Size: 988 281 741 246 3079
Fragments arranged by size:
3079 988 741 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5335
HpaI GTT'AAC
Cuts at: 2925 2925
Size: 5335
KasI G'GCGC_C
Cuts at: 2180 2180
Size: 5335
KpnI G_GTAC'C
Cuts at: 2660 2660
Size: 5335
MfeI C'AATT_G
Cuts at: 2934 2934
Size: 5335
MluI A'CGCG_T
Cuts at: 5211 5211
Size: 5335
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4449 4633 1305
Size: 3144 184 2007
Fragments arranged by size:
3144 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3513
Fragments arranged by size:
3513 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2202 2282 3293 3652 3811 518
Size: 648 1036 80 1011 359 159 2042
Fragments arranged by size:
2042 1036 1011 648 359 159 80
MspA1I CmG'CkG
Cuts at: 1606 2188 2751 3428 4576 4821 1606
Size: 582 563 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 582 563 245
NarI GG'CG_CC
Cuts at: 2181 2181
Size: 5335
NcoI C'CATG_G
Cuts at: 513 1065 2301 513
Size: 552 1236 3547
Fragments arranged by size:
3547 1236 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5335
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5335
NotI GC'GGCC_GC
Cuts at: 2782 2782
Size: 5335
NspI r_CATG'y
Cuts at: 1132 1229 2693 4238 1132
Size: 97 1464 1545 2229
Fragments arranged by size:
2229 1545 1464 97
PasI CC'CwG_GG
Cuts at: 1348 2084 2578 1348
Size: 736 494 4105
Fragments arranged by size:
4105 736 494
PciI A'CATG_T
Cuts at: 4234 4234
Size: 5335
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5335
PmeI GTTT'AAAC
Cuts at: 2639 2639
Size: 5335
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3404 3436 4917 4949 3404
Size: 32 1481 32 3790
Fragments arranged by size:
3790 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2135 2135
Size: 5335
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5335
PsiI TTA'TAA
Cuts at: 2905 2905
Size: 5335
PstI C_TGCA'G
Cuts at: 830 2687 830
Size: 1857 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5335
SacII CC_GC'GG
Cuts at: 2189 2189
Size: 5335
SalI G'TCGA_C
Cuts at: 1936 2677 1936
Size: 741 4594
SbfI CC_TGCA'GG
Cuts at: 2687 2687
Size: 5335
ScaI AGT'ACT
Cuts at: 3583 5295 3583
Size: 1712 3623
SexAI A'CCwGG_T
Cuts at: 2542 2542
Size: 5335
SfcI C'TryA_G
Cuts at: 826 1045 2683 3818 4499 4690 826
Size: 219 1638 1135 681 191 1471
Fragments arranged by size:
1638 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2061 2061
Size: 5335
SfoI GGC'GCC
Cuts at: 2182 2182
Size: 5335
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5335
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5335
SmaI CCC'GGG
Cuts at: 1872 2141 2336 2662 1872
Size: 269 195 326 4545
Fragments arranged by size:
4545 326 269 195
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2267 3439 4340 4602 820
Size: 197 926 324 1172 901 262 1553
Fragments arranged by size:
1553 1172 926 901 324 262 197
SnaBI TAC'GTA
Cuts at: 493 3126 493
Size: 2633 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5335
SphI G_CATG'C
Cuts at: 1132 2693 1132
Size: 1561 3774
SspI AAT'ATT
Cuts at: 5 52 3259 5
Size: 47 3207 2081
Fragments arranged by size:
3207 2081 47
StuI AGG'CCT
Cuts at: 1517 2528 1517
Size: 1011 4324
StyI C'CwwG_G
Cuts at: 513 1065 2301 2774 513
Size: 552 1236 473 3074
Fragments arranged by size:
3074 1236 552 473
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2530 2577 3362 3379 3532 3717 4162
Size: 1255 950 47 785 17 153 185 445
Cuts at: 4162 325
Size: 1498
Fragments arranged by size:
1498 1255 950 785 445 185 153 47
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3581 5293 96
Size: 274 80 33 51 1443 1604 1712 138
Fragments arranged by size:
1712 1604 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2213 2245 5295 5327 2213
Size: 32 3050 32 2221
Fragments arranged by size:
3050 2221 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5335
XbaI T'CTAG_A
Cuts at: 2671 2671
Size: 5335
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5335
XmaI C'CCGG_G
Cuts at: 1870 2139 2334 2660 1870
Size: 269 195 326 4545
Fragments arranged by size:
4545 326 269 195
XmnI GAAnn'nnTTC
Cuts at: 2378 3464 2378
Size: 1086 4249
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3143 276
Size: 53 83 186 936 1609 2468
Fragments arranged by size:
2468 1609 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SacII SalI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SanDI SapI SrfI SwaI XcmI