(Circular) (Six-base) MAPSORT of: pFN21AB8327.seq Check: 1050 from: 1 to: 5467 pFN21AB8327 5467 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3277 278 Size: 53 83 186 936 1741 2468 Fragments arranged by size: 2468 1741 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2810 1937 Size: 873 4594 Acc65I G'GTAC_C Cuts at: 2788 2788 Size: 5467 AclI AA'CG_TT Cuts at: 1563 3594 3967 1563 Size: 2031 373 3063 Fragments arranged by size: 3063 2031 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3530 4914 5182 1632 Size: 1898 1384 268 1917 Fragments arranged by size: 1917 1898 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5270 AflIII A'CryG_T Cuts at: 1203 4366 5343 1203 Size: 3163 977 1327 Fragments arranged by size: 3163 1327 977 AgeI A'CCGG_T Cuts at: 4298 4298 Size: 5467 AhdI GACnn_n'nnGTC Cuts at: 2134 4196 2134 Size: 2062 3405 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5467 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2529 1184 Size: 1345 4122 AlwNI CAG_nnn'CTG Cuts at: 3210 4782 3210 Size: 1572 3895 ApaI G_GGCC'C Cuts at: 2329 2329 Size: 5467 ApaLI G'TGCA_C Cuts at: 3524 4680 3524 Size: 1156 4311 ApoI r'AATT_y Cuts at: 1493 2776 3002 3154 5263 1493 Size: 1283 226 152 2109 1697 Fragments arranged by size: 2109 1697 1283 226 152 AseI AT'TA_AT Cuts at: 160 1032 4021 160 Size: 872 2989 1606 Fragments arranged by size: 2989 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5467 AvaI C'yCGr_G Cuts at: 1870 1943 2139 2181 2266 2792 1870 Size: 73 196 42 85 526 4545 Fragments arranged by size: 4545 526 196 85 73 42 BamHI G'GATC_C Cuts at: 2797 3164 2797 Size: 367 5100 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2788 4243 618 Size: 325 217 627 1001 1455 1842 Fragments arranged by size: 1842 1455 1001 627 325 217 BanII G_rGCy'C Cuts at: 1391 2068 2329 2786 1391 Size: 677 261 457 4072 Fragments arranged by size: 4072 677 457 261 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4496 BbvCI CC'TCA_GC Cuts at: 2654 2706 2654 Size: 52 5415 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3658 3692 1903 Size: 34 1721 34 3678 Fragments arranged by size: 3678 1721 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3359 4569 3359 Size: 1210 4257 BclI T'GATC_A Cuts at: 1548 2196 1548 Size: 648 4819 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2052 4078 136 Size: 107 122 71 631 605 380 2026 1525 Fragments arranged by size: 2026 1525 631 605 380 122 107 71 BglII A'GATC_T Cuts at: 2547 5462 2547 Size: 2915 2552 BlpI GC'TnA_GC Cuts at: 2042 2884 2042 Size: 842 4625 Bme1580I G_kGCm'C Cuts at: 1790 2329 3528 4684 1790 Size: 539 1199 1156 2573 Fragments arranged by size: 2573 1199 1156 539 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5467 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2156 3198 4156 Size: 416 53 83 399 24 739 1042 958 Cuts at: 4156 442 Size: 1753 Fragments arranged by size: 1753 1042 958 739 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5467 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2082 2481 4127 1239 Size: 843 399 1646 2579 Fragments arranged by size: 2579 1646 843 399 Bpu10I CC'TnA_GC Cuts at: 2654 2706 2654 Size: 52 5415 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3592 4457 4755 3592 Size: 865 298 4304 Fragments arranged by size: 4304 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2381 4130 882 Size: 617 882 1749 2219 Fragments arranged by size: 2219 1749 882 617 BsaAI yAC'GTr Cuts at: 493 1204 2115 3258 493 Size: 711 911 1143 2702 Fragments arranged by size: 2702 1143 911 711 BsaBI GATnn'nnATC Cuts at: 5461 5461 Size: 5467 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3274 3656 4291 275 Size: 53 83 186 936 1741 382 635 1451 Fragments arranged by size: 1741 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3900 4298 4572 4719 5130 3900 Size: 398 274 147 411 4237 Fragments arranged by size: 4237 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5242 Fragments arranged by size: 5242 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2276 2636 2639 2678 1186 Size: 1090 360 3 39 3975 Fragments arranged by size: 3975 1090 360 39 3 BseYI C'CCAG_C Cuts at: 2122 2190 2206 4670 5166 2122 Size: 68 16 2464 496 2423 Fragments arranged by size: 2464 2423 496 68 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2089 2771 1163 Size: 573 353 682 3859 Fragments arranged by size: 3859 682 573 353 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2917 3678 4706 1540 Size: 275 186 916 761 1028 2301 Fragments arranged by size: 2301 1028 916 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3528 3613 4684 3528 Size: 85 1071 4311 Fragments arranged by size: 4311 1071 85 BsmI GAATG_Cn' Cuts at: 2978 3071 2978 Size: 93 5374 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2068 2329 2786 3528 3613 4684 1391 Size: 399 278 261 457 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 457 399 278 261 85 BspEI T'CCGG_A Cuts at: 5130 5130 Size: 5467 BspHI T'CATG_A Cuts at: 3356 5086 3356 Size: 1730 3737 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2127 2822 844 Size: 1283 695 3489 Fragments arranged by size: 3489 1283 695 BsrBI CCG'CTC Cuts at: 1394 1823 3354 1394 Size: 429 1531 3507 Fragments arranged by size: 3507 1531 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3962 4136 65 Size: 1183 2714 174 1396 Fragments arranged by size: 2714 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4111 4298 1700 Size: 110 2301 187 2869 Fragments arranged by size: 2869 2301 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5467 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5467 BssSI C'ACGA_G Cuts at: 3527 4539 3527 Size: 1012 4455 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5467 BstBI TT'CG_AA Cuts at: 4271 4271 Size: 5467 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5467 BstYI r'GATC_y Cuts at: 1972 2547 2797 3164 3549 3566 5018 5104 5116 Size: 575 250 367 385 17 1452 86 12 Cuts at: 5116 5462 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 575 385 367 346 250 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2479 2596 2616 513 Size: 552 939 475 117 20 3364 Fragments arranged by size: 3364 939 552 475 117 20 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4728 Fragments arranged by size: 4728 683 56 BtsI GCAGTG_nn' Cuts at: 2239 2760 2983 3777 3797 2239 Size: 521 223 794 20 3909 Fragments arranged by size: 3909 794 521 223 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5467 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5432 DraI TTT'AAA Cuts at: 2771 2771 Size: 5467 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4474 809 Size: 507 3158 1802 Fragments arranged by size: 3158 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2404 2914 3803 Size: 54 1425 50 275 18 574 510 889 Cuts at: 3803 5432 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 574 510 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2914 1537 Size: 275 1102 4090 Fragments arranged by size: 4090 1102 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3397 1353 Size: 2044 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 2506 4054 4428 4574 387 Size: 1064 138 435 482 1548 374 146 1280 Fragments arranged by size: 1548 1280 1064 482 435 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2082 2481 3530 4127 4914 5182 1239 Size: 393 450 399 1049 597 787 268 1524 Fragments arranged by size: 1524 1049 787 597 450 399 393 268 EcoO109I rG'GnC_Cy Cuts at: 2325 2325 Size: 5467 EcoRI G'AATT_C Cuts at: 2776 3154 2776 Size: 378 5089 FspI TGC'GCA Cuts at: 3973 3973 Size: 5467 HaeII r_GCGC'y Cuts at: 1678 2180 2377 4614 1678 Size: 502 197 2237 2531 Fragments arranged by size: 2531 2237 502 197 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2055 2087 2120 2152 2342 Size: 32 135 32 483 32 33 32 190 Cuts at: 2342 2346 2374 2378 4108 4140 4182 4214 1373 Size: 4 28 4 1730 32 42 32 2626 Fragments arranged by size: 2626 1730 483 190 135 42 33 32 32 32 32 32 32 28 4 4 HincII GTy'rAC Cuts at: 669 1657 1938 2811 3057 669 Size: 988 281 873 246 3079 Fragments arranged by size: 3079 988 873 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5467 HpaI GTT'AAC Cuts at: 3057 3057 Size: 5467 KpnI G_GTAC'C Cuts at: 2792 2792 Size: 5467 MfeI C'AATT_G Cuts at: 3066 3066 Size: 5467 MluI A'CGCG_T Cuts at: 5343 5343 Size: 5467 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2364 4581 4765 1305 Size: 1059 2217 184 2007 Fragments arranged by size: 2217 2007 1059 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2406 10 Size: 54 1425 343 574 3071 Fragments arranged by size: 3071 1425 574 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3425 3784 3943 518 Size: 648 2259 359 159 2042 Fragments arranged by size: 2259 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2041 2194 2658 2883 3560 4708 4953 1606 Size: 435 153 464 225 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 464 435 245 225 153 NcoI C'CATG_G Cuts at: 513 1065 2004 2596 513 Size: 552 939 592 3384 Fragments arranged by size: 3384 939 592 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5467 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5467 NotI GC'GGCC_GC Cuts at: 2914 2914 Size: 5467 NspI r_CATG'y Cuts at: 1132 1229 2740 2825 4370 1132 Size: 97 1511 85 1545 2229 Fragments arranged by size: 2229 1545 1511 97 85 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5467 PciI A'CATG_T Cuts at: 4366 4366 Size: 5467 PflMI CCAn_nnn'nTGG Cuts at: 1108 2449 1108 Size: 1341 4126 PfoI T'CCnGG_A Cuts at: 2166 2560 2166 Size: 394 5073 PmeI GTTT'AAAC Cuts at: 2771 2771 Size: 5467 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3536 3568 5049 5081 3536 Size: 32 1481 32 3922 Fragments arranged by size: 3922 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5467 PsiI TTA'TAA Cuts at: 3037 3037 Size: 5467 PspOMI G'GGCC_C Cuts at: 2325 2325 Size: 5467 PstI C_TGCA'G Cuts at: 830 2819 830 Size: 1989 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5467 PvuII CAG'CTG Cuts at: 2041 2194 2658 2041 Size: 153 464 4850 Fragments arranged by size: 4850 464 153 SalI G'TCGA_C Cuts at: 1936 2809 1936 Size: 873 4594 SbfI CC_TGCA'GG Cuts at: 2819 2819 Size: 5467 ScaI AGT'ACT Cuts at: 3715 5427 3715 Size: 1712 3755 SfcI C'TryA_G Cuts at: 826 1045 2815 3950 4631 4822 826 Size: 219 1770 1135 681 191 1471 Fragments arranged by size: 1770 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5467 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5467 SmaI CCC'GGG Cuts at: 1872 2141 2794 1872 Size: 269 653 4545 Fragments arranged by size: 4545 653 269 SmlI C'TyrA_G Cuts at: 820 1017 1943 3571 4472 4734 820 Size: 197 926 1628 901 262 1553 Fragments arranged by size: 1628 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3258 493 Size: 2765 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5467 SphI G_CATG'C Cuts at: 1132 2825 1132 Size: 1693 3774 SspI AAT'ATT Cuts at: 5 52 3391 5 Size: 47 3339 2081 Fragments arranged by size: 3339 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5467 StyI C'CwwG_G Cuts at: 513 1065 2004 2596 2906 513 Size: 552 939 592 310 3074 Fragments arranged by size: 3074 939 592 552 310 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2114 2472 2524 2773 3494 3511 3664 Size: 1255 534 358 52 249 721 17 153 Cuts at: 3664 3849 4294 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 721 534 445 358 249 185 153 52 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2017 3713 5425 Size: 274 80 33 51 1443 40 1696 1712 Cuts at: 5425 96 Size: 138 Fragments arranged by size: 1712 1696 1443 274 138 80 51 40 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5427 5459 5427 Size: 32 5435 Tth111I GACn'n_nGTC Cuts at: 1316 2162 1316 Size: 846 4621 XbaI T'CTAG_A Cuts at: 2803 2803 Size: 5467 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5467 XmaI C'CCGG_G Cuts at: 1870 2139 2792 1870 Size: 269 653 4545 Fragments arranged by size: 4545 653 269 XmnI GAAnn'nnTTC Cuts at: 3596 3596 Size: 5467 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3275 276 Size: 53 83 186 936 1741 2468 Fragments arranged by size: 2468 1741 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI