(Circular) (Six-base) MAPSORT of: pFN21AB8326.seq Check: 5137 from: 1 to: 5803 pFN21AB8326 5803 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2248 2592 2248 Size: 344 5459 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3613 278 Size: 53 83 186 936 2077 2468 Fragments arranged by size: 2468 2077 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3146 1937 Size: 1209 4594 Acc65I G'GTAC_C Cuts at: 3124 3124 Size: 5803 AclI AA'CG_TT Cuts at: 1563 3930 4303 1563 Size: 2367 373 3063 Fragments arranged by size: 3063 2367 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3866 5250 5518 1632 Size: 2234 1384 268 1917 Fragments arranged by size: 2234 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5606 AflIII A'CryG_T Cuts at: 1203 4702 5679 1203 Size: 3499 977 1327 Fragments arranged by size: 3499 1327 977 AgeI A'CCGG_T Cuts at: 4634 4634 Size: 5803 AhdI GACnn_n'nnGTC Cuts at: 4532 4532 Size: 5803 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5803 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5803 AlwNI CAG_nnn'CTG Cuts at: 2023 2176 2356 3546 5118 2023 Size: 153 180 1190 1572 2708 Fragments arranged by size: 2708 1572 1190 180 153 ApaI G_GGCC'C Cuts at: 2674 2674 Size: 5803 ApaLI G'TGCA_C Cuts at: 2579 3860 5016 2579 Size: 1281 1156 3366 Fragments arranged by size: 3366 1281 1156 ApoI r'AATT_y Cuts at: 1493 3112 3338 3490 5599 1493 Size: 1619 226 152 2109 1697 Fragments arranged by size: 2109 1697 1619 226 152 AseI AT'TA_AT Cuts at: 160 1032 4357 160 Size: 872 3325 1606 Fragments arranged by size: 3325 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5803 AvaI C'yCGr_G Cuts at: 1870 1943 2570 3128 1870 Size: 73 627 558 4545 Fragments arranged by size: 4545 627 558 73 BamHI G'GATC_C Cuts at: 3133 3500 3133 Size: 367 5436 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2333 2495 2593 2917 3124 Size: 325 217 627 546 162 98 324 207 Cuts at: 3124 4579 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 546 325 324 217 207 162 98 BanII G_rGCy'C Cuts at: 1391 2674 2910 2985 3096 3122 1391 Size: 1283 236 75 111 26 4072 Fragments arranged by size: 4072 1283 236 111 75 26 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2622 928 Size: 971 723 4109 Fragments arranged by size: 4109 971 723 BbvCI CC'TCA_GC Cuts at: 2471 2471 Size: 5803 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3994 4028 1903 Size: 34 2057 34 3678 Fragments arranged by size: 3678 2057 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3695 4905 3695 Size: 1210 4593 BclI T'GATC_A Cuts at: 1548 1548 Size: 5803 BfrBI ATG'CAT Cuts at: 2302 2302 Size: 5803 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2186 2686 4414 Size: 107 122 71 631 605 514 500 1728 Cuts at: 4414 136 Size: 1525 Fragments arranged by size: 1728 1525 631 605 514 500 122 107 71 BglII A'GATC_T Cuts at: 5798 5798 Size: 5803 BlpI GC'TnA_GC Cuts at: 3220 3220 Size: 5803 Bme1580I G_kGCm'C Cuts at: 1790 2080 2217 2583 2598 2674 3002 3864 5020 Size: 290 137 366 15 76 328 862 1156 Cuts at: 5020 1790 Size: 2573 Fragments arranged by size: 2573 1156 862 366 328 290 137 76 15 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5803 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3534 4492 442 Size: 416 53 83 399 24 2117 958 1753 Fragments arranged by size: 2117 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5803 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2165 2685 2801 4463 1239 Size: 926 520 116 1662 2579 Fragments arranged by size: 2579 1662 926 520 116 Bpu10I CC'TnA_GC Cuts at: 2338 2471 2338 Size: 133 5670 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3928 4793 5091 3928 Size: 865 298 4640 Fragments arranged by size: 4640 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2144 2413 2548 4466 882 Size: 617 645 269 135 1918 2219 Fragments arranged by size: 2219 1918 645 617 269 135 BsaAI yAC'GTr Cuts at: 493 1204 3594 493 Size: 711 2390 2702 Fragments arranged by size: 2702 2390 711 BsaBI GATnn'nnATC Cuts at: 5797 5797 Size: 5803 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3048 3610 3992 4627 Size: 53 83 186 936 1515 562 382 635 Cuts at: 4627 275 Size: 1451 Fragments arranged by size: 1515 1451 936 635 562 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2803 4236 4634 4908 5055 5466 2803 Size: 1433 398 274 147 411 3140 Fragments arranged by size: 3140 1433 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2774 2804 1178 Size: 30 165 30 1371 30 4177 Fragments arranged by size: 4177 1371 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2162 2611 2727 2798 2829 2832 1186 Size: 976 449 116 71 31 3 4157 Fragments arranged by size: 4157 976 449 116 71 31 3 BseYI C'CCAG_C Cuts at: 2281 2717 2909 2913 3095 5006 5502 2281 Size: 436 192 4 182 1911 496 2582 Fragments arranged by size: 2582 1911 496 436 192 182 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2138 1163 Size: 573 402 4828 Fragments arranged by size: 4828 573 402 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2646 3253 4014 5042 1540 Size: 275 186 645 607 761 1028 2301 Fragments arranged by size: 2301 1028 761 645 607 275 186 BsiHKAI G_wGCw'C Cuts at: 2583 2985 3864 3949 5020 2583 Size: 402 879 85 1071 3366 Fragments arranged by size: 3366 1071 879 402 85 BsmI GAATG_Cn' Cuts at: 3314 3407 3314 Size: 93 5710 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2080 2217 2583 2598 2674 2910 2985 Size: 399 290 137 366 15 76 236 75 Cuts at: 2985 3002 3096 3122 3864 3949 5020 1391 Size: 17 94 26 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 366 290 236 137 94 85 76 75 26 17 15 BspEI T'CCGG_A Cuts at: 5466 5466 Size: 5803 BspHI T'CATG_A Cuts at: 3692 5422 3692 Size: 1730 4073 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2244 2248 2592 2841 3158 844 Size: 1400 4 344 249 317 3489 Fragments arranged by size: 3489 1400 344 317 249 4 BsrBI CCG'CTC Cuts at: 1394 1823 3690 1394 Size: 429 1867 3507 Fragments arranged by size: 3507 1867 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4298 4472 65 Size: 1183 3050 174 1396 Fragments arranged by size: 3050 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2587 2645 4447 4634 1700 Size: 110 777 58 1802 187 2869 Fragments arranged by size: 2869 1802 777 187 110 58 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5803 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5803 BssSI C'ACGA_G Cuts at: 3863 4875 3863 Size: 1012 4791 BstAPI GCAn_nnn'nTGC Cuts at: 794 2221 794 Size: 1427 4376 BstBI TT'CG_AA Cuts at: 4607 4607 Size: 5803 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5803 BstXI CCAn_nnnn'nTGG Cuts at: 2725 2725 Size: 5803 BstYI r'GATC_y Cuts at: 1972 2037 3133 3500 3885 3902 5354 5440 5452 Size: 65 1096 367 385 17 1452 86 12 Cuts at: 5452 5798 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1096 385 367 346 86 65 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2154 2455 513 Size: 552 939 150 301 3861 Fragments arranged by size: 3861 939 552 301 150 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2362 487 Size: 683 56 1136 3928 Fragments arranged by size: 3928 1136 683 56 BtsI GCAGTG_nn' Cuts at: 3319 4113 4133 3319 Size: 794 20 4989 Fragments arranged by size: 4989 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5803 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5768 DraI TTT'AAA Cuts at: 3107 3107 Size: 5803 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4810 809 Size: 507 3494 1802 Fragments arranged by size: 3494 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2047 2178 2208 Size: 54 1425 50 275 18 217 131 30 Cuts at: 2208 2365 2452 2643 3250 4139 5768 8 Size: 157 87 191 607 889 1629 43 Fragments arranged by size: 1629 1425 889 607 275 217 191 157 131 87 54 50 43 30 18 EagI C'GGCC_G Cuts at: 1537 1812 2643 3250 1537 Size: 275 831 607 4090 Fragments arranged by size: 4090 831 607 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2805 3733 1353 Size: 1452 928 3423 Fragments arranged by size: 3423 1452 928 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 2530 2602 4390 4764 4910 Size: 1064 138 435 506 72 1788 374 146 Cuts at: 4910 387 Size: 1280 Fragments arranged by size: 1788 1280 1064 506 435 374 146 138 72 EcoICRI GAG'CTC Cuts at: 2983 2983 Size: 5803 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2165 2685 2801 3866 4463 5250 5518 Size: 393 533 520 116 1065 597 787 268 Cuts at: 5518 1239 Size: 1524 Fragments arranged by size: 1524 1065 787 597 533 520 393 268 116 EcoNI CCTnn'n_nnAGG Cuts at: 2255 2413 2255 Size: 158 5645 EcoO109I rG'GnC_Cy Cuts at: 2467 2521 2670 2671 2467 Size: 54 149 1 5599 Fragments arranged by size: 5599 149 54 1 EcoRI G'AATT_C Cuts at: 3112 3490 3112 Size: 378 5425 FspI TGC'GCA Cuts at: 2225 4309 2225 Size: 2084 3719 HaeII r_GCGC'y Cuts at: 1678 2144 2655 4950 1678 Size: 466 511 2295 2531 Fragments arranged by size: 2531 2295 511 466 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2774 2806 4444 4476 4518 Size: 32 135 32 1202 32 1638 32 42 Cuts at: 4518 4550 1373 Size: 32 2626 Fragments arranged by size: 2626 1638 1202 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3147 3393 669 Size: 988 281 1209 246 3079 Fragments arranged by size: 3079 1209 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5803 HpaI GTT'AAC Cuts at: 3393 3393 Size: 5803 KpnI G_GTAC'C Cuts at: 3128 3128 Size: 5803 MfeI C'AATT_G Cuts at: 3402 3402 Size: 5803 MluI A'CGCG_T Cuts at: 5679 5679 Size: 5803 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2211 4917 5101 1305 Size: 906 2706 184 2007 Fragments arranged by size: 2706 2007 906 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2180 2454 10 Size: 54 1425 343 348 274 3359 Fragments arranged by size: 3359 1425 348 343 274 54 MslI CAynn'nnrTG Cuts at: 518 1166 2303 3761 4120 4279 518 Size: 648 1137 1458 359 159 2042 Fragments arranged by size: 2042 1458 1137 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2023 2353 2482 2767 2913 3099 3219 3896 Size: 417 330 129 285 146 186 120 677 Cuts at: 3896 5044 5289 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 417 330 285 245 186 146 129 120 NaeI GCC'GGC Cuts at: 2647 2647 Size: 5803 NcoI C'CATG_G Cuts at: 513 1065 2004 2154 2455 513 Size: 552 939 150 301 3861 Fragments arranged by size: 3861 939 552 301 150 NdeI CA'TA_TG Cuts at: 387 2304 387 Size: 1917 3886 NgoMIV G'CCGG_C Cuts at: 2645 2645 Size: 5803 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5803 NotI GC'GGCC_GC Cuts at: 3250 3250 Size: 5803 NsiI A_TGCA'T Cuts at: 2304 2304 Size: 5803 NspI r_CATG'y Cuts at: 1132 1229 2230 2302 3161 4706 1132 Size: 97 1001 72 859 1545 2229 Fragments arranged by size: 2229 1545 1001 859 97 72 PasI CC'CwG_GG Cuts at: 1348 3077 1348 Size: 1729 4074 PciI A'CATG_T Cuts at: 4702 4702 Size: 5803 PflMI CCAn_nnn'nTGG Cuts at: 1108 2176 2894 1108 Size: 1068 718 4017 Fragments arranged by size: 4017 1068 718 PmeI GTTT'AAAC Cuts at: 3107 3107 Size: 5803 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3872 3904 5385 5417 3872 Size: 32 1481 32 4258 Fragments arranged by size: 4258 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2467 2467 Size: 5803 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5803 PsiI TTA'TAA Cuts at: 3373 3373 Size: 5803 PspOMI G'GGCC_C Cuts at: 2670 2670 Size: 5803 PstI C_TGCA'G Cuts at: 830 2028 2241 2417 3155 830 Size: 1198 213 176 738 3478 Fragments arranged by size: 3478 1198 738 213 176 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5803 PvuII CAG'CTG Cuts at: 2023 2482 2913 3099 2023 Size: 459 431 186 4727 Fragments arranged by size: 4727 459 431 186 SacI G_AGCT'C Cuts at: 2985 2985 Size: 5803 SalI G'TCGA_C Cuts at: 1936 3145 1936 Size: 1209 4594 SapI GCTCTTCn'nnn_ Cuts at: 2805 2805 Size: 5803 SbfI CC_TGCA'GG Cuts at: 2241 3155 2241 Size: 914 4889 ScaI AGT'ACT Cuts at: 4051 5763 4051 Size: 1712 4091 SexAI A'CCwGG_T Cuts at: 2193 2193 Size: 5803 SfcI C'TryA_G Cuts at: 826 1045 2015 2024 2237 2413 3151 4286 4967 Size: 219 970 9 213 176 738 1135 681 Cuts at: 4967 5158 826 Size: 191 1471 Fragments arranged by size: 1471 1135 970 738 681 219 213 191 176 9 SfiI GGCCn_nnn'nGGCC Cuts at: 2186 2186 Size: 5803 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5803 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5803 SmaI CCC'GGG Cuts at: 1872 3130 1872 Size: 1258 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3907 4808 5070 820 Size: 197 926 1964 901 262 1553 Fragments arranged by size: 1964 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3594 493 Size: 3101 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5803 SphI G_CATG'C Cuts at: 1132 3161 1132 Size: 2029 3774 SspI AAT'ATT Cuts at: 5 52 3727 5 Size: 47 3675 2081 Fragments arranged by size: 3675 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5803 StyI C'CwwG_G Cuts at: 513 1065 2004 2154 2455 2547 2682 2724 3242 Size: 552 939 150 301 92 135 42 518 Cuts at: 3242 513 Size: 3074 Fragments arranged by size: 3074 939 552 518 301 150 135 92 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2905 3830 3847 4000 4185 4630 325 Size: 1255 1325 925 17 153 185 445 1498 Fragments arranged by size: 1498 1325 1255 925 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4049 5761 96 Size: 274 80 33 51 1443 2072 1712 138 Fragments arranged by size: 2072 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5763 5795 5763 Size: 32 5771 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5803 XbaI T'CTAG_A Cuts at: 3139 3139 Size: 5803 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5803 XmaI C'CCGG_G Cuts at: 1870 3128 1870 Size: 1258 4545 XmnI GAAnn'nnTTC Cuts at: 3932 3932 Size: 5803 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3611 276 Size: 53 83 186 936 2077 2468 Fragments arranged by size: 2468 2077 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI NruI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SfoI SrfI SwaI XcmI