Restriction map for
pFN21AB8323
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaHI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstNI BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DdeI DpnII DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRII
EcoRV EcoT14I FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HpaI KasI KpnI KspI
MaeI MboI MfeI MflI MluI
MroI MslI MunI MvaI MvnI
NaeI NarI NcoI NdeI NdeII
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacII SalI Sau3AI SbfI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tth111I
Van91I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AciI 16 CCGC CCGC(-3/-1)
AfaI 309 GTAC GT^AC
AflIII 523 ACACGT A^C[GA][CT]GT
AlwNI 445 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 58 GAGCTC G[AT]GC[AT]^C
AvaI 211 CCCGGG C^[CT]CG[GA]G
AvaII 215 GGACC G^G[AT]CC
BalI 602 TGGCCA TGG^CCA
BbvI 573 GCTGC GCAGC(8/12)
BsaAI 524 CACGTA [CT]AC^GT[GA]
BsaBI 454 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 427 GGTCTC GGTCTC(1/5)
BsaJI 211 CCCGGG C^C[ACGT][ACGT]GG
BsiHKAI 58 GAGCTC G[AT]GC[AT]^C
BsiYI 16 CCGCTGAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 16 CCGCTGAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 428 GTCTC GTCTC(1/5)
BsoBI 211 CCCGGG C^[CT]CG[GA]G
BspHI 125 TCATGA T^CATGA
BsrDI 542 GCAATG GCAATG(2/0)
Cfr13I 215 GGACC G^G[ACGT]CC
DraII 214 GGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 602 TGGCCA [CT]^GGCC[GA]
EcoO109I 214 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT22I 668 ATGCAT ATGCA^T
HapII 212 CCGG C^CGG
HincII 169 GTTGAC GT[CT]^[GA]AC
HindII 169 GTTGAC GT[CT]^[GA]AC
HindIII 316 AAGCTT A^AGCTT
HpaII 212 CCGG C^CGG
MaeII 525 ACGT A^CGT
MaeIII 160 GTGAC ^GT[ACGT]AC
MamI 454 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 602 TGGCCA TGG^CCA
MscI 602 TGGCCA TGG^CCA
MseI 313 TTAA T^TAA
MspA1I 16 CCGCTG C[AC]G^C[GT]G
MspI 212 CCGG C^CGG
NlaIII 126 CATG CATG^
NsiI 668 ATGCAT ATGCA^T
PleI 749 GACTC GAGTC(4/5)
PpuMI 214 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 125 TCATGA T^CATGA
RsaI 309 GTAC GT^AC
SacI 58 GAGCTC GAGCT^C
Sau96I 215 GGACC G^G[ACGT]CC
ScaI 308 AGTACT AGT^ACT
SmaI 211 CCCGGG CCC^GGG
Tru9I 313 TTAA T^TAA
TseI 573 GCTGC G^C[AT]GC
Tsp45I 160 GTGAC ^GT[GC]AC
XmaCI 211 CCCGGG C^CCGGG
XmaI 211 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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BanII 46 GAGCCC G[GA]GC[CT]^C
58 GAGCTC
BcnI 211 CCCGG CC^[GC]GG
212 CCGGG
BmyI 46 GAGCCC G[AGT]GC[ACT]^C
58 GAGCTC
BpmI 418 CTGGAG CTGGAG(16/14)
651 CTGGAG
BseRI 470 CTCCTC GAGGAG(10/8)
34 GAGGAG
Bsp1286I 46 GAGCCC G[AGT]GC[ACT]^C
58 GAGCTC
BspMI 194 ACCTGC ACCTGC(4/8)
569 ACCTGC
BssKI 211 CCCGG ^CC[ACGT]GG
212 CCGGG
Cac8I 73 GCTGGC GC[ACGT]^[ACGT]GC
186 GCCAGC
EcoRI 390 GAATTC G^AATTC
465 GAATTC
Fnu4HI 15 GCCGC GC^[ACGT]GC
573 GCTGC
HaeIII 398 GGCC GG^CC
603 GGCC
ItaI 15 GCCGC GC^[ACGT]GC
573 GCTGC
NciI 211 CCCGG CC^[GC]GG
212 CCGGG
PstI 196 CTGCAG CTGCA^G
442 CTGCAG
SapI 60 GCTCTTC GCTCTTC(1/4)
120 GCTCTTC
ScrFI 211 CCCGG CC^[ACGT]GG
212 CCGGG
SfaNI 12 GATGC GCATC(5/9)
647 GCATC
SfcI 196 CTGCAG C^T[GA][CT]AG
442 CTGCAG
TaqI 10 TCGA T^CGA
56 TCGA
TfiI 129 GATTC G^A[AT]TC
337 GAATC
TthHB8I 10 TCGA T^CGA
56 TCGA
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# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AcsI AluI ApoI BsmFI BsrI
BstF5I EarI FokI HinfI HphI
Ksp632I MboII MnlI MwoI NlaIV
Tsp509I TspRI