(Circular) (Six-base) MAPSORT of: pFN21AB8323.seq Check: 2996 from: 1 to: 5464 pFN21AB8323 5464 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3274 278 Size: 53 83 186 936 1738 2468 Fragments arranged by size: 2468 1738 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2807 1937 Size: 870 4594 Acc65I G'GTAC_C Cuts at: 2785 2785 Size: 5464 AclI AA'CG_TT Cuts at: 1563 3591 3964 1563 Size: 2028 373 3063 Fragments arranged by size: 3063 2028 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2054 3527 4911 5179 1632 Size: 422 1473 1384 268 1917 Fragments arranged by size: 1917 1473 1384 422 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5267 AflIII A'CryG_T Cuts at: 1203 2528 4363 5340 1203 Size: 1325 1835 977 1327 Fragments arranged by size: 1835 1327 1325 977 AgeI A'CCGG_T Cuts at: 4295 4295 Size: 5464 AhdI GACnn_n'nnGTC Cuts at: 4193 4193 Size: 5464 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5464 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2594 1184 Size: 1410 4054 AlwNI CAG_nnn'CTG Cuts at: 2455 3207 4779 2455 Size: 752 1572 3140 Fragments arranged by size: 3140 1572 752 ApaLI G'TGCA_C Cuts at: 3521 4677 3521 Size: 1156 4308 ApoI r'AATT_y Cuts at: 1493 2282 2375 2395 2470 2534 2773 2999 3151 Size: 789 93 20 75 64 239 226 152 Cuts at: 3151 5260 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 789 239 226 152 93 75 64 20 AseI AT'TA_AT Cuts at: 160 1032 4018 160 Size: 872 2986 1606 Fragments arranged by size: 2986 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5464 AvaI C'yCGr_G Cuts at: 1870 1943 2216 2789 1870 Size: 73 273 573 4545 Fragments arranged by size: 4545 573 273 73 BamHI G'GATC_C Cuts at: 2794 3161 2794 Size: 367 5097 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2785 4240 618 Size: 325 217 627 998 1455 1842 Fragments arranged by size: 1842 1455 998 627 325 217 BanII G_rGCy'C Cuts at: 1391 2055 2067 2783 1391 Size: 664 12 716 4072 Fragments arranged by size: 4072 716 664 12 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4493 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3655 3689 1903 Size: 34 1718 34 3678 Fragments arranged by size: 3678 1718 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3356 4566 3356 Size: 1210 4254 BclI T'GATC_A Cuts at: 1548 1548 Size: 5464 BfrBI ATG'CAT Cuts at: 2675 2675 Size: 5464 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4075 136 Size: 107 122 71 631 605 2403 1525 Fragments arranged by size: 2403 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5459 5459 Size: 5464 BlpI GC'TnA_GC Cuts at: 2881 2881 Size: 5464 Bme1580I G_kGCm'C Cuts at: 1790 3525 4681 1790 Size: 1735 1156 2573 Fragments arranged by size: 2573 1735 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5464 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2703 3195 4153 Size: 416 53 83 399 24 1286 492 958 Cuts at: 4153 442 Size: 1753 Fragments arranged by size: 1753 1286 958 492 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5464 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2444 2677 4124 1239 Size: 1205 233 1447 2579 Fragments arranged by size: 2579 1447 1205 233 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3589 4454 4752 3589 Size: 865 298 4301 Fragments arranged by size: 4301 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2438 4127 882 Size: 617 939 1689 2219 Fragments arranged by size: 2219 1689 939 617 BsaAI yAC'GTr Cuts at: 493 1204 2531 3255 493 Size: 711 1327 724 2702 Fragments arranged by size: 2702 1327 724 711 BsaBI GATnn'nnATC Cuts at: 2463 5458 2463 Size: 2995 2469 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3271 3653 4288 275 Size: 53 83 186 936 1738 382 635 1451 Fragments arranged by size: 1738 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3897 4295 4569 4716 5127 3897 Size: 398 274 147 411 4234 Fragments arranged by size: 4234 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5239 Fragments arranged by size: 5239 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2054 2466 1186 Size: 868 412 4184 Fragments arranged by size: 4184 868 412 BseYI C'CCAG_C Cuts at: 4667 5163 4667 Size: 496 4968 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4891 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2914 3675 4703 1540 Size: 275 186 913 761 1028 2301 Fragments arranged by size: 2301 1028 913 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2067 3525 3610 4681 2067 Size: 1458 85 1071 2850 Fragments arranged by size: 2850 1458 1071 85 BsmI GAATG_Cn' Cuts at: 2975 3068 2975 Size: 93 5371 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 2067 2783 3525 3610 4681 1391 Size: 399 265 12 716 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 716 399 265 85 12 BspEI T'CCGG_A Cuts at: 5127 5127 Size: 5464 BspHI T'CATG_A Cuts at: 2130 3353 5083 2130 Size: 1223 1730 2511 Fragments arranged by size: 2511 1730 1223 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2208 2583 2819 844 Size: 1364 375 236 3489 Fragments arranged by size: 3489 1364 375 236 BsrBI CCG'CTC Cuts at: 1394 1823 3351 1394 Size: 429 1528 3507 Fragments arranged by size: 3507 1528 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2554 3959 4133 65 Size: 1183 1306 1405 174 1396 Fragments arranged by size: 1405 1396 1306 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4108 4295 1700 Size: 110 2298 187 2869 Fragments arranged by size: 2869 2298 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5464 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5464 BssSI C'ACGA_G Cuts at: 3524 4536 3524 Size: 1012 4452 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5464 BstBI TT'CG_AA Cuts at: 4268 4268 Size: 5464 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5464 BstYI r'GATC_y Cuts at: 1972 2794 3161 3546 3563 5015 5101 5113 5459 Size: 822 367 385 17 1452 86 12 346 Cuts at: 5459 1972 Size: 1977 Fragments arranged by size: 1977 1452 822 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3973 Fragments arranged by size: 3973 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4725 Fragments arranged by size: 4725 683 56 BtsI GCAGTG_nn' Cuts at: 2089 2980 3774 3794 2089 Size: 891 794 20 3759 Fragments arranged by size: 3759 891 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5464 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5429 DraI TTT'AAA Cuts at: 2768 2768 Size: 5464 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4471 809 Size: 507 3155 1802 Fragments arranged by size: 3155 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2607 2911 3800 Size: 54 1425 50 275 18 777 304 889 Cuts at: 3800 5429 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 777 304 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2911 1537 Size: 275 1099 4090 Fragments arranged by size: 4090 1099 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2072 2132 2286 3394 1353 Size: 719 60 154 1108 3423 Fragments arranged by size: 3423 1108 719 154 60 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4051 4425 4571 387 Size: 1064 138 2462 374 146 1280 Fragments arranged by size: 2462 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2065 2065 Size: 5464 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2444 2677 3527 4124 4911 5179 Size: 393 422 390 233 850 597 787 268 Cuts at: 5179 1239 Size: 1524 Fragments arranged by size: 1524 850 787 597 422 393 390 268 233 EcoO109I rG'GnC_Cy Cuts at: 2220 2220 Size: 5464 EcoRI G'AATT_C Cuts at: 2395 2470 2773 3151 2395 Size: 75 303 378 4708 Fragments arranged by size: 4708 378 303 75 FspI TGC'GCA Cuts at: 3970 3970 Size: 5464 HaeII r_GCGC'y Cuts at: 1678 4611 1678 Size: 2933 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4105 4137 4179 4211 1373 Size: 32 135 32 2533 32 42 32 2626 Fragments arranged by size: 2626 2533 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2176 2808 3054 669 Size: 988 281 238 632 246 3079 Fragments arranged by size: 3079 988 632 281 246 238 HindIII A'AGCT_T Cuts at: 748 2321 748 Size: 1573 3891 HpaI GTT'AAC Cuts at: 3054 3054 Size: 5464 KpnI G_GTAC'C Cuts at: 2789 2789 Size: 5464 MfeI C'AATT_G Cuts at: 3063 3063 Size: 5464 MluI A'CGCG_T Cuts at: 5340 5340 Size: 5464 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4578 4762 1305 Size: 3273 184 2007 Fragments arranged by size: 3273 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2609 10 Size: 54 1425 343 777 2865 Fragments arranged by size: 2865 1425 777 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3422 3781 3940 518 Size: 648 2256 359 159 2042 Fragments arranged by size: 2256 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2023 2880 3557 4705 4950 1606 Size: 417 857 677 1148 245 2120 Fragments arranged by size: 2120 1148 857 677 417 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3973 Fragments arranged by size: 3973 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5464 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5464 NotI GC'GGCC_GC Cuts at: 2911 2911 Size: 5464 NsiI A_TGCA'T Cuts at: 2677 2677 Size: 5464 NspI r_CATG'y Cuts at: 1132 1229 2822 4367 1132 Size: 97 1593 1545 2229 Fragments arranged by size: 2229 1593 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5464 PciI A'CATG_T Cuts at: 4363 4363 Size: 5464 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5464 PmeI GTTT'AAAC Cuts at: 2768 2768 Size: 5464 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3533 3565 5046 5078 3533 Size: 32 1481 32 3919 Fragments arranged by size: 3919 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2220 2220 Size: 5464 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5464 PsiI TTA'TAA Cuts at: 2237 3034 2237 Size: 797 4667 PstI C_TGCA'G Cuts at: 830 2205 2451 2816 830 Size: 1375 246 365 3478 Fragments arranged by size: 3478 1375 365 246 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5464 SacI G_AGCT'C Cuts at: 2067 2067 Size: 5464 SalI G'TCGA_C Cuts at: 1936 2806 1936 Size: 870 4594 SanDI GG'GwC_CC Cuts at: 2220 2220 Size: 5464 SapI GCTCTTCn'nnn_ Cuts at: 2072 2132 2072 Size: 60 5404 SbfI CC_TGCA'GG Cuts at: 2816 2816 Size: 5464 ScaI AGT'ACT Cuts at: 2315 3712 5424 2315 Size: 1397 1712 2355 Fragments arranged by size: 2355 1712 1397 SfcI C'TryA_G Cuts at: 826 1045 2201 2447 2812 3947 4628 4819 826 Size: 219 1156 246 365 1135 681 191 1471 Fragments arranged by size: 1471 1156 1135 681 365 246 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5464 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5464 SmaI CCC'GGG Cuts at: 1872 2218 2791 1872 Size: 346 573 4545 Fragments arranged by size: 4545 573 346 SmlI C'TyrA_G Cuts at: 820 1017 1943 3568 4469 4731 820 Size: 197 926 1625 901 262 1553 Fragments arranged by size: 1625 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3255 493 Size: 2762 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5464 SphI G_CATG'C Cuts at: 1132 2822 1132 Size: 1690 3774 SspI AAT'ATT Cuts at: 5 52 3388 5 Size: 47 3336 2081 Fragments arranged by size: 3336 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5464 StyI C'CwwG_G Cuts at: 513 1065 2004 2760 2903 513 Size: 552 939 756 143 3074 Fragments arranged by size: 3074 939 756 552 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2241 2352 3491 3508 3661 3846 4291 Size: 1255 661 111 1139 17 153 185 445 Cuts at: 4291 325 Size: 1498 Fragments arranged by size: 1498 1255 1139 661 445 185 153 111 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2313 3710 5422 Size: 274 80 33 51 1443 336 1397 1712 Cuts at: 5422 96 Size: 138 Fragments arranged by size: 1712 1443 1397 336 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5424 5456 5424 Size: 32 5432 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5464 XbaI T'CTAG_A Cuts at: 2800 2800 Size: 5464 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5464 XmaI C'CCGG_G Cuts at: 1870 2216 2789 1870 Size: 346 573 4545 Fragments arranged by size: 4545 573 346 XmnI GAAnn'nnTTC Cuts at: 3593 3593 Size: 5464 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3272 276 Size: 53 83 186 936 1738 2468 Fragments arranged by size: 2468 1738 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI