Restriction map for
pFN21AB8323
(Linear) (Six-base) MAPSORT of: KIBB8323.seq Check: 5934 from: 1 to: 759
KIBB8323.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 759
Size: 49 710
AflIII A'CryG_T
Cuts at: 0 523 759
Size: 523 236
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 589 759
Size: 589 170
AlwNI CAG_nnn'CTG
Cuts at: 0 450 759
Size: 450 309
ApoI r'AATT_y
Cuts at: 0 277 370 390 465 529 759
Size: 277 93 20 75 64 230
Fragments arranged by size:
277 230 93 75 64 20
AvaI C'yCGr_G
Cuts at: 0 211 759
Size: 211 548
BanII G_rGCy'C
Cuts at: 0 50 62 759
Size: 50 12 697
Fragments arranged by size:
697 50 12
BfrBI ATG'CAT
Cuts at: 0 670 759
Size: 670 89
BmrI ACTGGGnnnn_n'
Cuts at: 0 698 759
Size: 698 61
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 672 759
Size: 439 233 87
Fragments arranged by size:
439 233 87
BsaI GGTCTCn'nnnn_
Cuts at: 0 433 759
Size: 433 326
BsaAI yAC'GTr
Cuts at: 0 526 759
Size: 526 233
BsaBI GATnn'nnATC
Cuts at: 0 458 759
Size: 458 301
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 461 759
Size: 49 412 298
Fragments arranged by size:
412 298 49
BsiHKAI G_wGCw'C
Cuts at: 0 62 759
Size: 62 697
Bsp1286I G_dGCh'C
Cuts at: 0 50 62 759
Size: 50 12 697
Fragments arranged by size:
697 50 12
BspHI T'CATG_A
Cuts at: 0 125 759
Size: 125 634
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 203 578 759
Size: 203 375 181
Fragments arranged by size:
375 203 181
BsrDI GCAATG_nn'
Cuts at: 0 549 759
Size: 549 210
BtsI GCAGTG_nn'
Cuts at: 0 84 759
Size: 84 675
EaeI y'GGCC_r
Cuts at: 0 602 759
Size: 602 157
EarI CTCTTCn'nnn_
Cuts at: 0 67 127 281 759
Size: 67 60 154 478
Fragments arranged by size:
478 154 67 60
EcoICRI GAG'CTC
Cuts at: 0 60 759
Size: 60 699
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 439 672 759
Size: 49 390 233 87
Fragments arranged by size:
390 233 87 49
EcoO109I rG'GnC_Cy
Cuts at: 0 215 759
Size: 215 544
EcoRI G'AATT_C
Cuts at: 0 390 465 759
Size: 390 75 294
Fragments arranged by size:
390 294 75
HincII GTy'rAC
Cuts at: 0 171 759
Size: 171 588
HindIII A'AGCT_T
Cuts at: 0 316 759
Size: 316 443
MscI TGG'CCA
Cuts at: 0 604 759
Size: 604 155
MspA1I CmG'CkG
Cuts at: 0 18 759
Size: 18 741
NsiI A_TGCA'T
Cuts at: 0 672 759
Size: 672 87
PpuMI rG'GwC_Cy
Cuts at: 0 215 759
Size: 215 544
PsiI TTA'TAA
Cuts at: 0 232 759
Size: 232 527
PstI C_TGCA'G
Cuts at: 0 200 446 759
Size: 200 246 313
Fragments arranged by size:
313 246 200
SacI G_AGCT'C
Cuts at: 0 62 759
Size: 62 697
SanDI GG'GwC_CC
Cuts at: 0 215 759
Size: 215 544
SapI GCTCTTCn'nnn_
Cuts at: 0 67 127 759
Size: 67 60 632
Fragments arranged by size:
632 67 60
ScaI AGT'ACT
Cuts at: 0 310 759
Size: 310 449
SfcI C'TryA_G
Cuts at: 0 196 442 759
Size: 196 246 317
Fragments arranged by size:
317 246 196
SmaI CCC'GGG
Cuts at: 0 213 759
Size: 213 546
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 236 347 759
Size: 236 111 412
Fragments arranged by size:
412 236 111
TatI w'GTAC_w
Cuts at: 0 308 759
Size: 308 451
XmaI C'CCGG_G
Cuts at: 0 211 759
Size: 211 548
Enzymes that do cut:
AcuI AflIII AlfI AlwNI ApoI AvaI BanII BfrBI
BmrI BpmI BsaI BsaAI BsaBI BseRI BsiHKAI Bsp1286I
BspHI BspMI BsrDI BtsI EaeI EarI EcoICRI Eco57MI
EcoO109I EcoRI HincII HindIII MscI MspA1I NsiI PpuMI
PsiI PstI SacI SanDI SapI ScaI SfcI SmaI
TaqII TatI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmtI
BplI Bpu10I BpuEI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoNI EcoRV FalI FseI FspI FspAI
HaeII Hin4I HpaI KasI KpnI MfeI MluI MmeI
MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PspOMI PspXI PsrI PvuI PvuII
RsrII SacII SalI SbfI SexAI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TstI Tth111I XbaI XcmI XhoI XmnI
ZraI