Restriction map for
pFN21AB8322
(Circular) (Six-base) MAPSORT of: pFN21AB8322.seq Check: 9159 from: 1 to: 6709
pFN21AB8322 6709 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4519 278
Size: 53 83 186 936 2983 2468
Fragments arranged by size:
2983 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3428 4052 1937
Size: 1491 624 4594
Fragments arranged by size:
4594 1491 624
Acc65I G'GTAC_C
Cuts at: 4030 4030
Size: 6709
AclI AA'CG_TT
Cuts at: 1563 4836 5209 1563
Size: 3273 373 3063
Fragments arranged by size:
3273 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2026 3346 4772 6156 6424 1632
Size: 394 1320 1426 1384 268 1917
Fragments arranged by size:
1917 1426 1384 1320 394 268
AfeI AGC'GCT
Cuts at: 2561 2666 2684 3710 2561
Size: 105 18 1026 5560
Fragments arranged by size:
5560 1026 105 18
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6512
AflIII A'CryG_T
Cuts at: 1203 2529 3174 3573 3823 5608 6585 1203
Size: 1326 645 399 250 1785 977 1327
Fragments arranged by size:
1785 1327 1326 977 645 399 250
AgeI A'CCGG_T
Cuts at: 5540 5540
Size: 6709
AhdI GACnn_n'nnGTC
Cuts at: 2467 5438 2467
Size: 2971 3738
AleI CACnn'nnGTG
Cuts at: 1166 2094 3588 3674 1166
Size: 928 1494 86 4201
Fragments arranged by size:
4201 1494 928 86
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2069 2158 1184
Size: 885 89 5735
Fragments arranged by size:
5735 885 89
AlwNI CAG_nnn'CTG
Cuts at: 2827 3379 3463 3615 4452 6024 2827
Size: 552 84 152 837 1572 3512
Fragments arranged by size:
3512 1572 837 552 152 84
ApaLI G'TGCA_C
Cuts at: 2350 2851 3523 3737 4766 5922 2350
Size: 501 672 214 1029 1156 3137
Fragments arranged by size:
3137 1156 1029 672 501 214
ApoI r'AATT_y
Cuts at: 1493 4018 4244 4396 6505 1493
Size: 2525 226 152 2109 1697
Fragments arranged by size:
2525 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5263 160
Size: 872 4231 1606
Fragments arranged by size:
4231 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6709
AvaI C'yCGr_G
Cuts at: 1870 1943 2057 2389 2404 2432 2702 2914 3011
Size: 73 114 332 15 28 270 212 97
Cuts at: 3011 4034 1870
Size: 1023 4545
Fragments arranged by size:
4545 1023 332 270 212 114 97 73
28 15
BamHI G'GATC_C
Cuts at: 2394 4039 4406 2394
Size: 1645 367 4697
Fragments arranged by size:
4697 1645 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2879 2936 3878 4030 5485
Size: 325 217 627 1092 57 942 152 1455
Cuts at: 5485 618
Size: 1842
Fragments arranged by size:
1842 1455 1092 942 627 325 217 152
57
BanII G_rGCy'C
Cuts at: 1391 2895 3728 4028 1391
Size: 1504 833 300 4072
Fragments arranged by size:
4072 1504 833 300
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2640 2865 928
Size: 971 741 225 4772
Fragments arranged by size:
4772 971 741 225
BbvCI CC'TCA_GC
Cuts at: 3036 3036
Size: 6709
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2341 2375 3002 3036 4900 4934 1903
Size: 34 404 34 627 34 1864 34 3678
Fragments arranged by size:
3678 1864 627 404 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4601 5811 4601
Size: 1210 5499
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6709
BfrBI ATG'CAT
Cuts at: 3994 3994
Size: 6709
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3897 5320 136
Size: 107 122 71 631 605 2225 1423 1525
Fragments arranged by size:
2225 1525 1423 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6704 6704
Size: 6709
BlpI GC'TnA_GC
Cuts at: 4126 4126
Size: 6709
Bme1580I G_kGCm'C
Cuts at: 1790 2354 2429 2855 2882 3527 3741 3883 4770
Size: 564 75 426 27 645 214 142 887
Cuts at: 4770 5926 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 887 645 564 426 214 142
75 27
BmgBI CAC'GTC
Cuts at: 1318 2445 3177 1318
Size: 1127 732 4850
Fragments arranged by size:
4850 1127 732
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3391 4440 5398
Size: 416 53 83 399 24 1974 1049 958
Cuts at: 5398 442
Size: 1753
Fragments arranged by size:
1974 1753 1049 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6709
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2965 3270 2965
Size: 305 6404
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3836 5369 1239
Size: 2597 1533 2579
Fragments arranged by size:
2597 2579 1533
Bpu10I CC'TnA_GC
Cuts at: 3036 3036
Size: 6709
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4834 5699 5997 4834
Size: 865 298 5546
Fragments arranged by size:
5546 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2054 2284 2699 5372 882
Size: 617 555 230 415 2673 2219
Fragments arranged by size:
2673 2219 617 555 415 230
BsaAI yAC'GTr
Cuts at: 493 1204 3826 4500 493
Size: 711 2622 674 2702
Fragments arranged by size:
2702 2622 711 674
BsaBI GATnn'nnATC
Cuts at: 6703 6703
Size: 6709
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2919 4516 4898 5533
Size: 53 83 186 936 1386 1597 382 635
Cuts at: 5533 275
Size: 1451
Fragments arranged by size:
1597 1451 1386 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3082 5142 5540 5814 5961 6372 3082
Size: 2060 398 274 147 411 3419
Fragments arranged by size:
3419 2060 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2519 2549 2948 2978 3920
Size: 30 165 30 1116 30 399 30 942
Cuts at: 3920 3950 1178
Size: 30 3937
Fragments arranged by size:
3937 1116 942 399 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2861 2917 1186
Size: 1675 56 4978
Fragments arranged by size:
4978 1675 56
BseYI C'CCAG_C
Cuts at: 2639 3204 3327 3414 3946 5912 6408 2639
Size: 565 123 87 532 1966 496 2940
Fragments arranged by size:
2940 1966 565 532 496 123 87
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2249 2840 3423 3818 3895 1163
Size: 573 513 591 583 395 77 3977
Fragments arranged by size:
3977 591 583 573 513 395 77
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2313 4159 4920 5948 1540
Size: 275 186 312 1846 761 1028 2301
Fragments arranged by size:
2301 1846 1028 761 312 275 186
BsiHKAI G_wGCw'C
Cuts at: 2058 2354 2537 2855 3527 3728 3741 4770 4855
Size: 296 183 318 672 201 13 1029 85
Cuts at: 4855 5926 2058
Size: 1071 2841
Fragments arranged by size:
2841 1071 1029 672 318 296 201 183
85 13
BsmI GAATG_Cn'
Cuts at: 2016 4220 4313 2016
Size: 2204 93 4412
Fragments arranged by size:
4412 2204 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2058 2354 2429 2537 2855 2882 2895
Size: 399 268 296 75 108 318 27 13
Cuts at: 2895 3527 3728 3741 3883 4028 4770 4855 5926
Size: 632 201 13 142 145 742 85 1071
Cuts at: 5926 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 632 399 318 296 268
201 145 142 108 85 75 27 13
13
BspEI T'CCGG_A
Cuts at: 3082 6372 3082
Size: 3290 3419
BspHI T'CATG_A
Cuts at: 4598 6328 4598
Size: 1730 4979
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2487 3268 3315 4064 844
Size: 1643 781 47 749 3489
Fragments arranged by size:
3489 1643 781 749 47
BsrBI CCG'CTC
Cuts at: 1394 1823 4596 1394
Size: 429 2773 3507
Fragments arranged by size:
3507 2773 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3100 3191 3853 5204 5378 65
Size: 1183 1852 91 662 1351 174 1396
Fragments arranged by size:
1852 1396 1351 1183 662 174 91
BsrFI r'CCGG_y
Cuts at: 1700 1810 2156 5353 5540 1700
Size: 110 346 3197 187 2869
Fragments arranged by size:
3197 2869 346 187 110
BsrGI T'GTAC_A
Cuts at: 96 2259 96
Size: 2163 4546
BssHII G'CGCG_C
Cuts at: 1925 2131 1925
Size: 206 6503
BssSI C'ACGA_G
Cuts at: 2594 4769 5781 2594
Size: 2175 1012 3522
Fragments arranged by size:
3522 2175 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2236 2818 3193 3531 794
Size: 1442 582 375 338 3972
Fragments arranged by size:
3972 1442 582 375 338
BstBI TT'CG_AA
Cuts at: 5513 5513
Size: 6709
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6709
BstYI r'GATC_y
Cuts at: 1972 2208 2301 2394 2511 2823 4039 4406 4791
Size: 236 93 93 117 312 1216 367 385
Cuts at: 4791 4808 6260 6346 6358 6704 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1216 385 367 346 312 236
117 93 93 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3245 3245
Size: 6709
BtgI C'CryG_G
Cuts at: 513 1065 2083 2116 2420 3333 513
Size: 552 1018 33 304 913 3889
Fragments arranged by size:
3889 1018 913 552 304 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2394 487
Size: 683 56 1168 4802
Fragments arranged by size:
4802 1168 683 56
BtsI GCAGTG_nn'
Cuts at: 2586 2713 3346 4225 5019 5039 2586
Size: 127 633 879 794 20 4256
Fragments arranged by size:
4256 879 794 633 127 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6709
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6674
DraI TTT'AAA
Cuts at: 4013 4013
Size: 6709
DraIII CAC_nnn'GTG
Cuts at: 2503 3661 2503
Size: 1158 5551
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2918 3149 5716 809
Size: 507 1602 231 2567 1802
Fragments arranged by size:
2567 1802 1602 507 231
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2086 2310 4156
Size: 54 1425 50 275 18 256 224 1846
Cuts at: 4156 5045 6674 8
Size: 889 1629 43
Fragments arranged by size:
1846 1629 1425 889 275 256 224 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2310 4156 1537
Size: 275 498 1846 4090
Fragments arranged by size:
4090 1846 498 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3323 3365 3716 4639 1353
Size: 1970 42 351 923 3423
Fragments arranged by size:
3423 1970 923 351 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5296 5670 5816 387
Size: 1064 138 3707 374 146 1280
Fragments arranged by size:
3707 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3726 3726
Size: 6709
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2026 3346 3836 4772 5369 6156 6424
Size: 393 394 1320 490 936 597 787 268
Cuts at: 6424 1239
Size: 1524
Fragments arranged by size:
1524 1320 936 787 597 490 394 393
268
EcoO109I rG'GnC_Cy
Cuts at: 3852 3852
Size: 6709
EcoRI G'AATT_C
Cuts at: 4018 4396 4018
Size: 378 6331
FspI TGC'GCA
Cuts at: 2111 2741 5215 2111
Size: 630 2474 3605
Fragments arranged by size:
3605 2474 630
FspAI rTGC'GCAy
Cuts at: 2111 2741 2111
Size: 630 6079
HaeII r_GCGC'y
Cuts at: 1678 2563 2668 2686 3455 3625 3712 5856 1678
Size: 885 105 18 769 170 87 2144 2531
Fragments arranged by size:
2531 2144 885 769 170 105 87 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2198 2230 2453 2485 5350
Size: 32 135 32 626 32 223 32 2865
Cuts at: 5350 5382 5424 5456 1373
Size: 32 42 32 2626
Fragments arranged by size:
2865 2626 626 223 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3172 3429 4053 4299 669
Size: 988 281 1234 257 624 246 3079
Fragments arranged by size:
3079 1234 988 624 281 257 246
HindIII A'AGCT_T
Cuts at: 748 2905 748
Size: 2157 4552
HpaI GTT'AAC
Cuts at: 4299 4299
Size: 6709
KpnI G_GTAC'C
Cuts at: 4034 4034
Size: 6709
MfeI C'AATT_G
Cuts at: 4308 4308
Size: 6709
MluI A'CGCG_T
Cuts at: 2529 6585 2529
Size: 4056 2653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2268 3165 5823 6007 1305
Size: 963 897 2658 184 2007
Fragments arranged by size:
2658 2007 963 897 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2088 10
Size: 54 1425 343 256 4631
Fragments arranged by size:
4631 1425 343 256 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2094 3588 3674 3688 3989 4667 5026
Size: 648 928 1494 86 14 301 678 359
Cuts at: 5026 5185 518
Size: 159 2042
Fragments arranged by size:
2042 1494 928 678 648 359 301 159
86 14
MspA1I CmG'CkG
Cuts at: 1606 2142 2265 2422 2553 2574 2643 3035 3293
Size: 536 123 157 131 21 69 392 258
Cuts at: 3293 3335 3445 4125 4802 5950 6195 1606
Size: 42 110 680 677 1148 245 2120
Fragments arranged by size:
2120 1148 680 677 536 392 258 245
157 131 123 110 69 42 21
NaeI GCC'GGC
Cuts at: 2158 2158
Size: 6709
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6157
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6709
NgoMIV G'CCGG_C
Cuts at: 2156 2156
Size: 6709
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6709
NotI GC'GGCC_GC
Cuts at: 4156 4156
Size: 6709
NsiI A_TGCA'T
Cuts at: 3996 3996
Size: 6709
NspI r_CATG'y
Cuts at: 1132 1229 2128 3577 3745 3998 4067 5612 1132
Size: 97 899 1449 168 253 69 1545 2229
Fragments arranged by size:
2229 1545 1449 899 253 168 97 69
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6709
PciI A'CATG_T
Cuts at: 3573 5608 3573
Size: 2035 4674
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2629 1108
Size: 1521 5188
PfoI T'CCnGG_A
Cuts at: 2172 2397 2826 3813 2172
Size: 225 429 987 5068
Fragments arranged by size:
5068 987 429 225
PmeI GTTT'AAAC
Cuts at: 4013 4013
Size: 6709
PmlI CAC'GTG
Cuts at: 3826 3826
Size: 6709
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4778 4810 6291 6323 4778
Size: 32 1481 32 5164
Fragments arranged by size:
5164 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3852 3852
Size: 6709
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6709
PsiI TTA'TAA
Cuts at: 4279 4279
Size: 6709
PstI C_TGCA'G
Cuts at: 830 2301 2328 2484 3025 3865 4061 830
Size: 1471 27 156 541 840 196 3478
Fragments arranged by size:
3478 1471 840 541 196 156 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6709
PvuII CAG'CTG
Cuts at: 2643 3293 3445 2643
Size: 650 152 5907
Fragments arranged by size:
5907 650 152
SacI G_AGCT'C
Cuts at: 3728 3728
Size: 6709
SacII CC_GC'GG
Cuts at: 2423 3336 2423
Size: 913 5796
SalI G'TCGA_C
Cuts at: 1936 3427 4051 1936
Size: 1491 624 4594
Fragments arranged by size:
4594 1491 624
SanDI GG'GwC_CC
Cuts at: 3852 3852
Size: 6709
SapI GCTCTTCn'nnn_
Cuts at: 3716 3716
Size: 6709
SbfI CC_TGCA'GG
Cuts at: 3865 4061 3865
Size: 196 6513
ScaI AGT'ACT
Cuts at: 4957 6669 4957
Size: 1712 4997
SfcI C'TryA_G
Cuts at: 826 1045 2194 2297 2324 2480 3021 3861 4057
Size: 219 1149 103 27 156 541 840 196
Cuts at: 4057 5192 5873 6064 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1149 1135 840 681 541 219 196
191 156 103 27
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6709
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6709
SmaI CCC'GGG
Cuts at: 1872 2391 4036 1872
Size: 519 1645 4545
Fragments arranged by size:
4545 1645 519
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2057 4813 5714 5976 820
Size: 197 926 114 2756 901 262 1553
Fragments arranged by size:
2756 1553 926 901 262 197 114
SnaBI TAC'GTA
Cuts at: 493 4500 493
Size: 4007 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6709
SphI G_CATG'C
Cuts at: 1132 2128 4067 1132
Size: 996 1939 3774
Fragments arranged by size:
3774 1939 996
SspI AAT'ATT
Cuts at: 5 52 4633 5
Size: 47 4581 2081
Fragments arranged by size:
4581 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6709
StyI C'CwwG_G
Cuts at: 513 1065 4148 513
Size: 552 3083 3074
Fragments arranged by size:
3083 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2024 2505 3020 3157 3731 4736 4753
Size: 1255 444 481 515 137 574 1005 17
Cuts at: 4753 4906 5091 5536 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1005 574 515 481 445 444
185 153 137 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2259 4955 6667
Size: 274 80 33 51 1443 282 2696 1712
Cuts at: 6667 96
Size: 138
Fragments arranged by size:
2696 1712 1443 282 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6669 6701 6669
Size: 32 6677
Tth111I GACn'n_nGTC
Cuts at: 1316 3409 1316
Size: 2093 4616
XbaI T'CTAG_A
Cuts at: 4045 4045
Size: 6709
XhoI C'TCGA_G
Cuts at: 1943 2057 1943
Size: 114 6595
XmaI C'CCGG_G
Cuts at: 1870 2389 4034 1870
Size: 519 1645 4545
Fragments arranged by size:
4545 1645 519
XmnI GAAnn'nnTTC
Cuts at: 4838 4838
Size: 6709
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4517 276
Size: 53 83 186 936 2983 2468
Fragments arranged by size:
2983 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BsiWI
BsmBI BstXI BstZ17I EcoNI EcoRV FalI FseI KasI
NarI NruI PacI PspOMI PspXI PsrI RsrII SexAI
SfiI SfoI SrfI SwaI XcmI